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pbad24 plasmid  (ATCC)


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    Structured Review

    ATCC pbad24 plasmid
    Pbad24 Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20064 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbad24+plasmid/Plasmid/pm41688823-401-51-54
    Average 99 stars, based on 20064 article reviews
    pbad24 plasmid - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.
    Article Snippet: .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions
    Article Snippet: After ethanol precipitation, the total RNA preparation was converted into sequencing libraries with the NEBNext Ultra II directional RNA library prep kit for Illumina. .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Polymerase Chain Reaction:

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.
    Article Snippet: .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions
    Article Snippet: After ethanol precipitation, the total RNA preparation was converted into sequencing libraries with the NEBNext Ultra II directional RNA library prep kit for Illumina. .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Amplification:

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.
    Article Snippet: .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions
    Article Snippet: After ethanol precipitation, the total RNA preparation was converted into sequencing libraries with the NEBNext Ultra II directional RNA library prep kit for Illumina. .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Plasmid Preparation:

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.
    Article Snippet: .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions
    Article Snippet: After ethanol precipitation, the total RNA preparation was converted into sequencing libraries with the NEBNext Ultra II directional RNA library prep kit for Illumina. .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Clone Assay:

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.
    Article Snippet: .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions
    Article Snippet: After ethanol precipitation, the total RNA preparation was converted into sequencing libraries with the NEBNext Ultra II directional RNA library prep kit for Illumina. .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Control:

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions.
    Article Snippet: .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..

    Article Title: Sequence and chemical specificity define the functional landscape of intrinsically disordered regions
    Article Snippet: After ethanol precipitation, the total RNA preparation was converted into sequencing libraries with the NEBNext Ultra II directional RNA library prep kit for Illumina. .. The DNA sequence coding for the M.SssI methyltransferase, where the TGA stop codons coding for tryptophan in the original organism were replaced by TGG codons, was PCR amplified from the plasmid pCMV-FLAG-4azf-M.SssI (a gift from K. Ford at Kings College London) and cloned into the NcoI and SalI sites of the pBAD24 plasmid (87399; American Type Culture Collection) to achieve tight transcriptional control by the E. coli arabinose-regulated araBAD promoter. ..



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    Image Search Results


    (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a T4 RNA Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.

    Journal: bioRxiv

    Article Title: RNA-RNA Interactome Approaches Provide in vivo Evidence for a Critical Role of the Hfq Rim Face in sRNA-mRNA Pairing

    doi: 10.1101/2025.06.26.660752

    Figure Lengend Snippet: (A) Schematic overview of Hi-GRIL-seq and iRIL-seq workflows. WT (XL2001) and the hfq R16A mutant (FDM1050) were transformed with either an empty vector (pBAD24) or a T4 RNA Ligase I-expressing vector (pBAD24- t4rnl1 ). Biological duplicates of each strain were cultured in LB (Lennox) with ampicillin at 37°C until reaching an OD600 of ∼0.5, at which point arabinose was added to induce enzyme expression. Cells were then grown to early stationary phase (OD600 ∼1.0), harvested, and processed for either Hfq IP followed by RNA isolation (iRIL-seq) or direct RNA extraction for total RNA sequencing (Hi-GRIL-seq). sRNAs are shown in orange, mRNAs in black, tRNA in green and rRNA in blue. This figure was created in BioRender ( https://BioRender.com/jqqlsju ). (B) Circos plots illustrating RNA interactomes in WT and R16A Hfq with either pBAD24 or pBAD24- t4rnl1 , as detected by iRIL-seq. (C) Quantification of S-chimeras under different conditions (Supplementary Table S11). Table displaying the number of RNA pairs with significant chimeric fragments identified by iRIL-seq, with values shown for two biological replicates and their calculated mean. (D) Chimera levels of specific RNA pairs identified by RIL-seq (anti-Hfq) and iRIL-seq. The RNA pairs shown here include the six pairs analyzed in previous experiments as well as four additional pairs identified as having significant numbers of chimeras in the iRIL-seq R16A experiment. Chimera counts were extracted from Supplementary Table S6 (RIL-seq, anti-Hfq) and Supplementary Table S11 (iRIL-seq), using the same method described in . For each RNA pair, the values represent the mean from two biological replicates.

    Article Snippet: The T4 RNA Ligase I-expressing plasmid pBAD24- t4rnl1 was synthesized by the GenScript company.

    Techniques: Mutagenesis, Transformation Assay, Plasmid Preparation, Expressing, Cell Culture, Isolation, RNA Extraction, RNA Sequencing