nbp1 32440 antibody  (Novus Biologicals)

Journal: bioRxiv

Article Title: Systems serology of responses against tumor antigens in ovarian cancer reveal disrupted Fc-mediated immunity

doi: 10.64898/2026.04.25.720834

Figure Lengend Snippet: A) Anti-human IgG (left) immunohistochemistry (IHC) staining of an HGSOC primary solid tumor specimen from patient 9. Anti-PAX8 staining (right) is included as a tumor-specific lineage marker. Scale bars represent 50 µm or 100 µm, where noted. B) Anti-human IgG (top) IHC staining of an HGSOC ascites specimen from patient 56. Anti-PAX8 staining (bottom) of the same ascites specimen from patient 56. Scale bars represent 50 µm or 100 µm, where noted. C) Quantification of the staining across patients with available samples in the cohort (n=29). Samples with heterogeneous staining were assigned a range instead of a single score. D) Visual depiction of our hypothesis. While TBAs bind to tumor cells, their Fc properties may prevent interaction with the FcγRs on immune cells. E) The ratio of FcγRIIIa to FcγRIIa binding across patients, separated by Ab antigenic target (n=61). Fluorescence is indicated by relative fluorescence units (RFU). In E), significance was determined between the TBAs and anti-viral Abs using a Kruskal-Wallis H Test followed by Dunn’s post-hoc test with Bonferroni corrections. Here, “*” and “**” represent p-values of less than 0.05 and 0.0005, respectively. See also Figure S1 and Table S1.

Article Snippet: Sections were incubated in blocking buffer and stained with the following antibodies: PAX8 (Cell Marque #363M-15) and IgG (Dako #GA512).

Techniques: Immunohistochemistry, Staining, Marker, Binding Assay, Fluorescence