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receptors tlrs are pattern recognition receptors prrs  (Takeda)

 
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    Structured Review

    Takeda receptors tlrs are pattern recognition receptors prrs
    Receptors Tlrs Are Pattern Recognition Receptors Prrs, supplied by Takeda, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pattern+recognition+receptors+(prrs)/2004+k+like+receptor+signalling+toll/pm41552968-21-1-32
    Average 86 stars, based on 1 article reviews
    receptors tlrs are pattern recognition receptors prrs - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Bacteria:

    Article Title: Organic dust exposure induced pulmonary damage among livestock workers.
    Article Snippet: .. Recognition of micro-organisms is an important function of the innate and adaptive immunity and pattern recognition receptors (PRRs) recognize a variety of pathogen-associated molecular patterns (PAMPs) from viruses, bacteria, fungi and parasites (Takeda et al. 2003). ..

    Fungus:

    Article Title: Toll signalling controls intestinal regeneration in Drosophila.
    Article Snippet: .. Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) that recognize conserved microbial molecules like lipopolysaccharide (LPS), bacterial DNA, bacterial peptidoglycans, zymosan and betaglucan from fungi, and single-stranded and double-stranded RNA from viruses (Takeda andAkira, 2015). ..

    other:

    Article Title: Development of molecular and cellular tools to decipher the type I IFN pathway of the common vampire bat.
    Article Snippet: In mammals, innate immune recognition depends on the detection of constitutive and conserved patterns in microbes, the pathogenassociated molecular patterns (PAMPs), by a variety of pattern recognition receptors (PRRs) (Takeda et al., 2003).

    Article Title: Deciphering the tripartite interaction of urbanized environment, gut microbiome and cardio-metabolic disease.
    Article Snippet: The world is experiencing a sudden surge in urban population, especially in developing Asian and African countries.. Consequently, the global burden of cardio-metabolic disease (CMD) is also rising owing to gut microbiome dysbiosis due to urbanization factors such as mode of birth, breastfeeding, diet, environmental pollutants, and soil exposure.. Dysbiotic gut microbiome indicated by altered Firmicutes to Bacteroides ratio and loss of beneficial short-chain fatty acids-producing bacteria such as Prevotella, and Ruminococcus may disrupt host-intestinal homeostasis by altering host immune response, gut barrier integrity, and microbial metabolism through altered T-regulatory cells/T-helper cells balance, activation of pattern recognition receptors and toll-like receptors, decreased mucus production, elevated level of trimethylamine-oxide and primary bile acids.

    Article Title: Anti-colitis effect of Lactobacillus sakei K040706 via suppression of inflammatory responses in the dextran sulfate sodium-induced colitis mice model
    Article Snippet: Lactobacillus strains are used in the functional food industry as promising probiotics with purported beneficial effects.. We recently reported on the immunostimulatory effects of Lactobacillus sakei K040706 (K040706) in macrophages and in a cyclophosphamide-induced immunosuppression model. And this study assessed the anti-inflammatory effects and molecular mechanisms involving K040706 in a mouse model of colitis with dextran sodium sulfate (DSS).. K040706 significantly attenuated the clinical signs and histological characteristics in DSS-induced colitic mice, which are well-correlated with the restoration of tight junction protein expression and the significant reduction of immune cell infiltration and expression of ICAM-1 and VCAM-1.



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    Investigating the sexual dimorphism in the TLR7-, TLR9- and STING-mediated innate cytokine responses with aging . (a) demographic data of the study cohort, age and sex distribution. (b) Schematic summary of study design: Blood samples were obtained from 310 healthy community-dwelling individuals (ages 18–97), samples were separated in two for whole blood stimulation with the indicated PRR ligands, and the other part was used to isolate PBMCs for FACS analysis as indicated. (c, d, e) Levels of cytokine release after whole blood stimulation with the three studied PRR ligands: R848 (c), ODN M362 (d) or cGAMP (e). Concentrations of indicated cytokines were quantified at 24 h in plasma. The results are given in log 10 of the concentrations (pg/ml) with background (without ligands) subtracted. (c, d, e, f-h) Sex-differences in IFN-α release in response to R848 (f), ODN M362 (g) and cGAMP (h), before and after the age of 60. Statistical differences between groups were analyzed using the unpaired Student's t -test (c-d) or by a one-way ANOVA test followed by Sidak's multiple comparisons test (f–h).

    Journal: eBioMedicine

    Article Title: Monocytes are the main source of STING-mediated IFN-α production

    doi: 10.1016/j.ebiom.2022.104047

    Figure Lengend Snippet: Investigating the sexual dimorphism in the TLR7-, TLR9- and STING-mediated innate cytokine responses with aging . (a) demographic data of the study cohort, age and sex distribution. (b) Schematic summary of study design: Blood samples were obtained from 310 healthy community-dwelling individuals (ages 18–97), samples were separated in two for whole blood stimulation with the indicated PRR ligands, and the other part was used to isolate PBMCs for FACS analysis as indicated. (c, d, e) Levels of cytokine release after whole blood stimulation with the three studied PRR ligands: R848 (c), ODN M362 (d) or cGAMP (e). Concentrations of indicated cytokines were quantified at 24 h in plasma. The results are given in log 10 of the concentrations (pg/ml) with background (without ligands) subtracted. (c, d, e, f-h) Sex-differences in IFN-α release in response to R848 (f), ODN M362 (g) and cGAMP (h), before and after the age of 60. Statistical differences between groups were analyzed using the unpaired Student's t -test (c-d) or by a one-way ANOVA test followed by Sidak's multiple comparisons test (f–h).

    Article Snippet: Stock solutions of pattern recognition receptor (PRR) ligands (Resiquimod (R848) (Cat# tlrl-r848), ODN M362 (a class C CpG ODN, (Cat# tlrl-m362-5) and Cyclic [G(2’,5’)pA(3’,5’)p] cGAMP, (Cat# tlrl-nacga23-1) all purchased from InvivoGen, Toulouse, France) were prepared in RPMI.

    Techniques: Blood/Plasma/Serum Samples, Analysis, Clinical Proteomics, DNA Extraction, Flow Cytometry, Whole Blood Assay, Enzyme-linked Immunosorbent Assay, FACS

    The female predominance in TLR7-driven production of IFN-α is robustly maintained in elderly subjects above 80 . (a–c) From the source groups depicted in a (age range >80), the production of IFN-α is shown in response to R848 (a), ODN M362 (b) and cGAMP (c) by sex, female (F), male (M). Statistical differences between groups were analyzed using the unpaired Student's t -test .

    Journal: eBioMedicine

    Article Title: Monocytes are the main source of STING-mediated IFN-α production

    doi: 10.1016/j.ebiom.2022.104047

    Figure Lengend Snippet: The female predominance in TLR7-driven production of IFN-α is robustly maintained in elderly subjects above 80 . (a–c) From the source groups depicted in a (age range >80), the production of IFN-α is shown in response to R848 (a), ODN M362 (b) and cGAMP (c) by sex, female (F), male (M). Statistical differences between groups were analyzed using the unpaired Student's t -test .

    Article Snippet: Stock solutions of pattern recognition receptor (PRR) ligands (Resiquimod (R848) (Cat# tlrl-r848), ODN M362 (a class C CpG ODN, (Cat# tlrl-m362-5) and Cyclic [G(2’,5’)pA(3’,5’)p] cGAMP, (Cat# tlrl-nacga23-1) all purchased from InvivoGen, Toulouse, France) were prepared in RPMI.

    Techniques:

    Impact of TLR7 and CXorf21 SNPs on the IFN-α response to the three PPR ligands studied . Individuals were genotyped for two TLR7 SNPs (rs179008 (Gln11Leu) and rs3853839 (in 3′ UTR) (a, b) and the rs887369 CXorf21 SNP (c). For each SNP, the IFN-α responses observed for female homozygous for the most frequent alleles were compared to the responses of males having the corresponding alleles. The results are given in log 10 of the concentrations (pg/ml) with background (without ligands) subtracted. The unpaired Student's t -test was used to compare observations between male and female groups.

    Journal: eBioMedicine

    Article Title: Monocytes are the main source of STING-mediated IFN-α production

    doi: 10.1016/j.ebiom.2022.104047

    Figure Lengend Snippet: Impact of TLR7 and CXorf21 SNPs on the IFN-α response to the three PPR ligands studied . Individuals were genotyped for two TLR7 SNPs (rs179008 (Gln11Leu) and rs3853839 (in 3′ UTR) (a, b) and the rs887369 CXorf21 SNP (c). For each SNP, the IFN-α responses observed for female homozygous for the most frequent alleles were compared to the responses of males having the corresponding alleles. The results are given in log 10 of the concentrations (pg/ml) with background (without ligands) subtracted. The unpaired Student's t -test was used to compare observations between male and female groups.

    Article Snippet: Stock solutions of pattern recognition receptor (PRR) ligands (Resiquimod (R848) (Cat# tlrl-r848), ODN M362 (a class C CpG ODN, (Cat# tlrl-m362-5) and Cyclic [G(2’,5’)pA(3’,5’)p] cGAMP, (Cat# tlrl-nacga23-1) all purchased from InvivoGen, Toulouse, France) were prepared in RPMI.

    Techniques:

    Influence of CMV serological status on the decrease of IFN-α release with aging . IFN-α release expressed as in in response to stimulation with R848 is shown according to the CMV status: CMV positive (CMV pos) and CMV negative (CMV neg) individuals (a) or as a function of age (b). The Pearson's R coefficient and correlation probabilities were calculated by means of XLSTAT. Panel (c) shows the IFN-α production in CMV neg and CMV pos individuals by sex. (d) Production of IFN-α in CMV pos elderly subjects above 80. Differences between groups were analyzed using the Student's t-test (a, d) or using a one-way ANOVA test followed by Sidak's multiple comparisons test (c).

    Journal: eBioMedicine

    Article Title: Monocytes are the main source of STING-mediated IFN-α production

    doi: 10.1016/j.ebiom.2022.104047

    Figure Lengend Snippet: Influence of CMV serological status on the decrease of IFN-α release with aging . IFN-α release expressed as in in response to stimulation with R848 is shown according to the CMV status: CMV positive (CMV pos) and CMV negative (CMV neg) individuals (a) or as a function of age (b). The Pearson's R coefficient and correlation probabilities were calculated by means of XLSTAT. Panel (c) shows the IFN-α production in CMV neg and CMV pos individuals by sex. (d) Production of IFN-α in CMV pos elderly subjects above 80. Differences between groups were analyzed using the Student's t-test (a, d) or using a one-way ANOVA test followed by Sidak's multiple comparisons test (c).

    Article Snippet: Stock solutions of pattern recognition receptor (PRR) ligands (Resiquimod (R848) (Cat# tlrl-r848), ODN M362 (a class C CpG ODN, (Cat# tlrl-m362-5) and Cyclic [G(2’,5’)pA(3’,5’)p] cGAMP, (Cat# tlrl-nacga23-1) all purchased from InvivoGen, Toulouse, France) were prepared in RPMI.

    Techniques:

    Aging-related changes in pDCs and monocytes differ between sexes . (a) Absolute cell counts of pDC, mDC and monocytes per mm 3 were calculated from FCM data as described in materials and methods. Absolute counts per mm 3 (given in log 10 ) are compared in males and females for individuals under or above 60. (b, c) Correlation between log 10 IFN-α release in response to R848 (b) or ODN M362 (c) stimulation and log 10 absolute numbers per mm 3 of plasmacytoid dendritic cells. Pearson's correlation coefficients “ R” and probabilities of correlation were calculated by means of Statview software. (d, e) Residual values (difference between the observed IFN-α concentration and the predicted value) were calculated from the linear regression of IFN-α response as a function of pDC number shown in (b) and (c), respectively. Differences between groups were analyzed using a one-way ANOVA test followed by Sidak's multiple comparisons test (a, d, e).

    Journal: eBioMedicine

    Article Title: Monocytes are the main source of STING-mediated IFN-α production

    doi: 10.1016/j.ebiom.2022.104047

    Figure Lengend Snippet: Aging-related changes in pDCs and monocytes differ between sexes . (a) Absolute cell counts of pDC, mDC and monocytes per mm 3 were calculated from FCM data as described in materials and methods. Absolute counts per mm 3 (given in log 10 ) are compared in males and females for individuals under or above 60. (b, c) Correlation between log 10 IFN-α release in response to R848 (b) or ODN M362 (c) stimulation and log 10 absolute numbers per mm 3 of plasmacytoid dendritic cells. Pearson's correlation coefficients “ R” and probabilities of correlation were calculated by means of Statview software. (d, e) Residual values (difference between the observed IFN-α concentration and the predicted value) were calculated from the linear regression of IFN-α response as a function of pDC number shown in (b) and (c), respectively. Differences between groups were analyzed using a one-way ANOVA test followed by Sidak's multiple comparisons test (a, d, e).

    Article Snippet: Stock solutions of pattern recognition receptor (PRR) ligands (Resiquimod (R848) (Cat# tlrl-r848), ODN M362 (a class C CpG ODN, (Cat# tlrl-m362-5) and Cyclic [G(2’,5’)pA(3’,5’)p] cGAMP, (Cat# tlrl-nacga23-1) all purchased from InvivoGen, Toulouse, France) were prepared in RPMI.

    Techniques: Software, Concentration Assay

    Monocytes are the main source of IFN-α in response to cGAMP . Impact of aging on monocyte counts (a) and IFN-α response (b) after cGAMP stimulation for males and females. Correlation coefficients “R” and probabilities of correlation were calculated using Pearson test. (a) Correlation between the absolute number of monocytes/mm 3 (log 10 ) and age (years). (b) Correlation between the cGAMP-stimulated release of IFN-α (log 10 ) and the absolute number of monocytes/mm 3 (log 10 ). (c) PBMC from 5 donors were depleted of CD14 + cells (> 98% depletion) using magnetic particles or mock-depleted and stimulated with cGAMP (20 µg/ml) or R848 (1 µg/ml) for 24 h. IFN-α production was measured by ELISA. Paired Student's t -test was used for statistical analysis. (d, e) Total PBMCs (5 × 10 5 cells/well) or monocyte-enriched cells (% CD14 + cells > 77% at 3 × 10 5 cells/well) were stimulated with cGAMP or left untreated (NS). The production of IFN-α (d) or IP-10/CXCL-10 (e) was then assessed in 24 h-supernatants. Median values in pg/ml for [IFN-α] or [IP-10] concentrations normalized to 3 × 10 5 cells/well are shown in parentheses. (f, g) Intracellular analysis of IFN-α synthesis in 18 h-stimulated monocytes, gated as CD11c pos HLA-DR pos cells, from male or female donors. Brefeldin A was added for the last 3 h of culture. Frequencies of IFN-α pos monocytes are shown from individual female or male donors stimulated or not with cGAMP. Statistical differences between groups were assessed using the Kruskal-Wallis's test corrected for multiple comparisons.

    Journal: eBioMedicine

    Article Title: Monocytes are the main source of STING-mediated IFN-α production

    doi: 10.1016/j.ebiom.2022.104047

    Figure Lengend Snippet: Monocytes are the main source of IFN-α in response to cGAMP . Impact of aging on monocyte counts (a) and IFN-α response (b) after cGAMP stimulation for males and females. Correlation coefficients “R” and probabilities of correlation were calculated using Pearson test. (a) Correlation between the absolute number of monocytes/mm 3 (log 10 ) and age (years). (b) Correlation between the cGAMP-stimulated release of IFN-α (log 10 ) and the absolute number of monocytes/mm 3 (log 10 ). (c) PBMC from 5 donors were depleted of CD14 + cells (> 98% depletion) using magnetic particles or mock-depleted and stimulated with cGAMP (20 µg/ml) or R848 (1 µg/ml) for 24 h. IFN-α production was measured by ELISA. Paired Student's t -test was used for statistical analysis. (d, e) Total PBMCs (5 × 10 5 cells/well) or monocyte-enriched cells (% CD14 + cells > 77% at 3 × 10 5 cells/well) were stimulated with cGAMP or left untreated (NS). The production of IFN-α (d) or IP-10/CXCL-10 (e) was then assessed in 24 h-supernatants. Median values in pg/ml for [IFN-α] or [IP-10] concentrations normalized to 3 × 10 5 cells/well are shown in parentheses. (f, g) Intracellular analysis of IFN-α synthesis in 18 h-stimulated monocytes, gated as CD11c pos HLA-DR pos cells, from male or female donors. Brefeldin A was added for the last 3 h of culture. Frequencies of IFN-α pos monocytes are shown from individual female or male donors stimulated or not with cGAMP. Statistical differences between groups were assessed using the Kruskal-Wallis's test corrected for multiple comparisons.

    Article Snippet: Stock solutions of pattern recognition receptor (PRR) ligands (Resiquimod (R848) (Cat# tlrl-r848), ODN M362 (a class C CpG ODN, (Cat# tlrl-m362-5) and Cyclic [G(2’,5’)pA(3’,5’)p] cGAMP, (Cat# tlrl-nacga23-1) all purchased from InvivoGen, Toulouse, France) were prepared in RPMI.

    Techniques: Enzyme-linked Immunosorbent Assay, Analysis, FACS

    Detection of critical pattern recognition receptors (PRRs) in MLE-12 cells and mTECs by flow cytometry. ( A ) Strategy used to detect TLR2, TLR4, NOD1, and NOD2 expression in MLE-12 cells. ( B ) Quantitative analysis of PRR expression as % live cells. ( C ) Strategy used to detect the expression of TLR2, TLR4, NOD1, and NOD2 in CD326 + mTECs. ( D ) Quantitative analysis of PRR expression as % live CD326 + cells.

    Journal: Viruses

    Article Title: Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation

    doi: 10.3390/v13010002

    Figure Lengend Snippet: Detection of critical pattern recognition receptors (PRRs) in MLE-12 cells and mTECs by flow cytometry. ( A ) Strategy used to detect TLR2, TLR4, NOD1, and NOD2 expression in MLE-12 cells. ( B ) Quantitative analysis of PRR expression as % live cells. ( C ) Strategy used to detect the expression of TLR2, TLR4, NOD1, and NOD2 in CD326 + mTECs. ( D ) Quantitative analysis of PRR expression as % live CD326 + cells.

    Article Snippet: In similar experiments, MLE-12 cells were treated for 2 h with pattern recognition receptor (PRR) agonists (final concentration, 10 ng/mL; Invivogen), including Pam2CSK4 (TLR2/6), Pam3CSK4 (TLR1/2), MDP (NOD2), and L18-MDP (NOD2), or CL-429 (Pam2-MDP, dual TLR2/NOD2) or diluent control.

    Techniques: Flow Cytometry, Expressing