Review



pas2 1 vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    TaKaRa pas2 1 vector
    Pas2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pas2+1+vector/pmCherry-1+Vector/pmc02644286-154-22-24
    Average 96 stars, based on 484 article reviews
    pas2 1 vector - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Construct:

    Article Title: Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
    Article Snippet: The Matchmaker two-hybrid system 2 was used according to the protocol recommended by the manufacturer (Clontech Laboratories, Palo Alto, CA, USA). .. The full-length Haspin cDNA was constructed into a pAS2-1 vector (Clontech Laboratories, Palo Alto, CA, USA) and was transformed into competent yeast Y190 cells. ..

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. Mouse Evc in pAS2-1 vector was transformed into yeast strain AH109 and used as a bait to screen approximately 1.25 × 10 6 clones from a mouse 11-day embryo cDNA library constructed in the pACT2 vector and pre-transformed into yeast strain Y187 (Clontech, Mountain View, CA, USA). ..

    Plasmid Preparation:

    Article Title: Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
    Article Snippet: The Matchmaker two-hybrid system 2 was used according to the protocol recommended by the manufacturer (Clontech Laboratories, Palo Alto, CA, USA). .. The full-length Haspin cDNA was constructed into a pAS2-1 vector (Clontech Laboratories, Palo Alto, CA, USA) and was transformed into competent yeast Y190 cells. ..

    Article Title: Characterization of a novel transcriptionally active domain in the transforming growth factor β-regulated Smad3 protein
    Article Snippet: .. For the construction of the yeast expression vectors, the wild-type Smad3 and the Smad3 143–248 mutant were cloned into the pAS2-1 vector (Clontech) in frame with the DBD of GAL4. ..

    Article Title: MCRS2 represses the transactivation activities of Nrf1
    Article Snippet: CMV- LacZ was constructed by inserting the LacZ gene between the SmaI and BamHI sites of pRK5F (BD PharMingen). .. The DNA fragments encoding the C-terminal of Nrf1 (276–447), containing CNC and bZIP domain, was generated by PCR and subsequently inserted into pAS2-1 vector (Clontech) to produce GAL4DB-Nrf1 (276–447) bait. .. The GAL4DB-Nrf1 (276–447) bait was used to screen against human HeLa MATCHMAKER cDNA library (MATCHMAKER two-hybrid system; Clontech).

    Article Title: Heterogeneous Nuclear Ribonucleoprotein K Interacts with Abi-1 at Postsynaptic Sites and Modulates Dendritic Spine Morphology
    Article Snippet: .. The YTH screen was carried out with the full coding sequence of Abi-1 as bait that was fused in frame to the GAL4 DNA binding domain in the pAS2-1 vector (Clontech) and transformed to screen against a human fetal brain cDNA-library cloned into the pACT2 vector (Clontech). ..

    Article Title: GSK3beta-Mediated Drp1 Phosphorylation Induced Elongated Mitochondrial Morphology against Oxidative Stress
    Article Snippet: .. These amplified fragments were digested by BamHI and XhoI, and they were also introduced into the BamHI and XhoI sites of the pACT2 or pAS2-1 vector (MATCHMAKER Two-Hybrid System 2, Clontech). ..

    Transformation Assay:

    Article Title: Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids
    Article Snippet: The Matchmaker two-hybrid system 2 was used according to the protocol recommended by the manufacturer (Clontech Laboratories, Palo Alto, CA, USA). .. The full-length Haspin cDNA was constructed into a pAS2-1 vector (Clontech Laboratories, Palo Alto, CA, USA) and was transformed into competent yeast Y190 cells. ..

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. Mouse Evc in pAS2-1 vector was transformed into yeast strain AH109 and used as a bait to screen approximately 1.25 × 10 6 clones from a mouse 11-day embryo cDNA library constructed in the pACT2 vector and pre-transformed into yeast strain Y187 (Clontech, Mountain View, CA, USA). ..

    Article Title: Heterogeneous Nuclear Ribonucleoprotein K Interacts with Abi-1 at Postsynaptic Sites and Modulates Dendritic Spine Morphology
    Article Snippet: .. The YTH screen was carried out with the full coding sequence of Abi-1 as bait that was fused in frame to the GAL4 DNA binding domain in the pAS2-1 vector (Clontech) and transformed to screen against a human fetal brain cDNA-library cloned into the pACT2 vector (Clontech). ..

    Library Screening:

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. For yeast two-hybrid library screening, mouse Evc sequence encoding amino acids 49 - 1005 which does not include the transmembrane domain was cloned into pAS2-1 vector (Clontech, Mountain View, CA, USA). .. For directed yeast two-hybrid analysis, mouse Evc fragments were cloned into pGBKT7 vector (Clontech, Mountain View, CA, USA) and mouse Evc2 fragments were cloned into pGADT7 vector (Clontech, Mountain View, CA, USA).

    Sequencing:

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. For yeast two-hybrid library screening, mouse Evc sequence encoding amino acids 49 - 1005 which does not include the transmembrane domain was cloned into pAS2-1 vector (Clontech, Mountain View, CA, USA). .. For directed yeast two-hybrid analysis, mouse Evc fragments were cloned into pGBKT7 vector (Clontech, Mountain View, CA, USA) and mouse Evc2 fragments were cloned into pGADT7 vector (Clontech, Mountain View, CA, USA).

    Article Title: Heterogeneous Nuclear Ribonucleoprotein K Interacts with Abi-1 at Postsynaptic Sites and Modulates Dendritic Spine Morphology
    Article Snippet: .. The YTH screen was carried out with the full coding sequence of Abi-1 as bait that was fused in frame to the GAL4 DNA binding domain in the pAS2-1 vector (Clontech) and transformed to screen against a human fetal brain cDNA-library cloned into the pACT2 vector (Clontech). ..

    Clone Assay:

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. For yeast two-hybrid library screening, mouse Evc sequence encoding amino acids 49 - 1005 which does not include the transmembrane domain was cloned into pAS2-1 vector (Clontech, Mountain View, CA, USA). .. For directed yeast two-hybrid analysis, mouse Evc fragments were cloned into pGBKT7 vector (Clontech, Mountain View, CA, USA) and mouse Evc2 fragments were cloned into pGADT7 vector (Clontech, Mountain View, CA, USA).

    Article Title: Characterization of a novel transcriptionally active domain in the transforming growth factor β-regulated Smad3 protein
    Article Snippet: .. For the construction of the yeast expression vectors, the wild-type Smad3 and the Smad3 143–248 mutant were cloned into the pAS2-1 vector (Clontech) in frame with the DBD of GAL4. ..

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. Mouse Evc in pAS2-1 vector was transformed into yeast strain AH109 and used as a bait to screen approximately 1.25 × 10 6 clones from a mouse 11-day embryo cDNA library constructed in the pACT2 vector and pre-transformed into yeast strain Y187 (Clontech, Mountain View, CA, USA). ..

    Article Title: Heterogeneous Nuclear Ribonucleoprotein K Interacts with Abi-1 at Postsynaptic Sites and Modulates Dendritic Spine Morphology
    Article Snippet: .. The YTH screen was carried out with the full coding sequence of Abi-1 as bait that was fused in frame to the GAL4 DNA binding domain in the pAS2-1 vector (Clontech) and transformed to screen against a human fetal brain cDNA-library cloned into the pACT2 vector (Clontech). ..

    Expressing:

    Article Title: Characterization of a novel transcriptionally active domain in the transforming growth factor β-regulated Smad3 protein
    Article Snippet: .. For the construction of the yeast expression vectors, the wild-type Smad3 and the Smad3 143–248 mutant were cloned into the pAS2-1 vector (Clontech) in frame with the DBD of GAL4. ..

    Mutagenesis:

    Article Title: Characterization of a novel transcriptionally active domain in the transforming growth factor β-regulated Smad3 protein
    Article Snippet: .. For the construction of the yeast expression vectors, the wild-type Smad3 and the Smad3 143–248 mutant were cloned into the pAS2-1 vector (Clontech) in frame with the DBD of GAL4. ..

    cDNA Library Assay:

    Article Title: Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus
    Article Snippet: .. Mouse Evc in pAS2-1 vector was transformed into yeast strain AH109 and used as a bait to screen approximately 1.25 × 10 6 clones from a mouse 11-day embryo cDNA library constructed in the pACT2 vector and pre-transformed into yeast strain Y187 (Clontech, Mountain View, CA, USA). ..

    Generated:

    Article Title: MCRS2 represses the transactivation activities of Nrf1
    Article Snippet: CMV- LacZ was constructed by inserting the LacZ gene between the SmaI and BamHI sites of pRK5F (BD PharMingen). .. The DNA fragments encoding the C-terminal of Nrf1 (276–447), containing CNC and bZIP domain, was generated by PCR and subsequently inserted into pAS2-1 vector (Clontech) to produce GAL4DB-Nrf1 (276–447) bait. .. The GAL4DB-Nrf1 (276–447) bait was used to screen against human HeLa MATCHMAKER cDNA library (MATCHMAKER two-hybrid system; Clontech).

    Polymerase Chain Reaction:

    Article Title: MCRS2 represses the transactivation activities of Nrf1
    Article Snippet: CMV- LacZ was constructed by inserting the LacZ gene between the SmaI and BamHI sites of pRK5F (BD PharMingen). .. The DNA fragments encoding the C-terminal of Nrf1 (276–447), containing CNC and bZIP domain, was generated by PCR and subsequently inserted into pAS2-1 vector (Clontech) to produce GAL4DB-Nrf1 (276–447) bait. .. The GAL4DB-Nrf1 (276–447) bait was used to screen against human HeLa MATCHMAKER cDNA library (MATCHMAKER two-hybrid system; Clontech).

    Binding Assay:

    Article Title: Heterogeneous Nuclear Ribonucleoprotein K Interacts with Abi-1 at Postsynaptic Sites and Modulates Dendritic Spine Morphology
    Article Snippet: .. The YTH screen was carried out with the full coding sequence of Abi-1 as bait that was fused in frame to the GAL4 DNA binding domain in the pAS2-1 vector (Clontech) and transformed to screen against a human fetal brain cDNA-library cloned into the pACT2 vector (Clontech). ..

    Amplification:

    Article Title: GSK3beta-Mediated Drp1 Phosphorylation Induced Elongated Mitochondrial Morphology against Oxidative Stress
    Article Snippet: .. These amplified fragments were digested by BamHI and XhoI, and they were also introduced into the BamHI and XhoI sites of the pACT2 or pAS2-1 vector (MATCHMAKER Two-Hybrid System 2, Clontech). ..



    Similar Products

    96
    TaKaRa pas2 1 vector
    Pas2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pas2+1+vector/pmCherry-1+Vector/pmc02644286-154-22-24
    Average 96 stars, based on 1 article reviews
    pas2 1 vector - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    TaKaRa yeast gal4 dna binding domain expression vector pas2 1
    Yeast Gal4 Dna Binding Domain Expression Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pas2+1+vector/pmCherry+Vector/pmc03695902-56-6-14
    Average 96 stars, based on 1 article reviews
    yeast gal4 dna binding domain expression vector pas2 1 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    TaKaRa vector pas2 1
    Identification of LMO2 as an interaction partner for hBex2
    Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pas2+1+vector/pmCherry-1+Vector/pmc01298925-46-32-34
    Average 96 stars, based on 1 article reviews
    vector pas2 1 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    TaKaRa domain expression vector pas2 1
    Identification of LMO2 as an interaction partner for hBex2
    Domain Expression Vector Pas2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pas2+1+vector/pmCherry+Vector/pmc02679751-225-11-15
    Average 96 stars, based on 1 article reviews
    domain expression vector pas2 1 - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    Identification of LMO2 as an interaction partner for hBex2

    Journal: Nucleic Acids Research

    Article Title: Human Bex2 interacts with LMO2 and regulates the transcriptional activity of a novel DNA-binding complex

    doi: 10.1093/nar/gki964

    Figure Lengend Snippet: Identification of LMO2 as an interaction partner for hBex2

    Article Snippet: The full-length cDNAs for hBex1, hBex2, hBex3(NADE), mBex1, mBex2, mBex3 and various deletion mutants of hBex2 used in the yeast two-hybrid assay were cloned in frame with the GAL4 DNA-binding domain into vector pAS2-1 (BD_Clontech, CA).

    Techniques: Plasmid Preparation