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parental thp1  (ATCC)


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    ATCC parental thp1
    Parental Thp1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20342 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/parental+thp1/THP-1/pm41720222-68-0-9
    Average 99 stars, based on 20342 article reviews
    parental thp1 - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Detection of Cell Impurities and NF-κB activation Using SEAP Reporter Cell Lines in Vaccines.
    Article Snippet: Introduction: During the 2010 influenza season, the Therapeutic Goods Administration (TGA) reviewed adverse event (AE) signals of febrile convulsions in children following the administration of the seasonal Fluvax trivalent vaccine.. These AEs were attributed to nuclear factor kappa-beta (NF-κB) activation.. In response, the TGA developed a method to detect NF-κB activation by vaccine samples.



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    parent thp1 cells  (ATCC)
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    Human MAIT cells are activated by Legionella infection via MR1 in vitro. a Jurkat.MAIT and C1R.MR1 cells were co-incubated for 16 h with lysates of L. pneumophila (L. pn.) or L. longbeachae or 5-OP-RU, acetyl-6-formylpterin (Ac-6-FP) or PBS. Activation, detected by staining with anti-CD69, is enhanced by bacterial lysate or by the activating ligand 5-OP-RU, but not by acetyl-6-FP. Activation was blocked by anti-MR1 antibody (26.5) but not by isotype control (W6/32) 2 h prior to co-incubation. Experiment performed in triplicate wells on two separate occasions with similar results. Data show mean fluorescence intensity, MFI (±SEM). Statistical tests: one-way ANOVA and post hoc Dunnett’s comparing all columns with the first column (black). Unpaired t -test (blue), with *** P < 0.001; **** P < 0.0001. b , c <t>THP1</t> cells (WT) or THP1 cells overexpressing MR1 (THP1.MR1+, purple) or deficient in expression of MR1 (THP1.MR1−, blue) were infected for 27 h with live or heat-killed (HK) L. longbeachae (MOI: 100) or 10 nM 5-OP-RU, then co-cultured for 16 h with sorted CD3 + Vα7.2 + CD161 + human peripheral blood MAIT cells, or MAIT-depleted conventional T cells. MR1-5-OP-RU-tetramer+ MAIT cell activation was measured by intracellular cytokine staining for b TNF or c IFN-γ. b , c Percentage cytokine-positive cells as mean (±SEM) data from three independent donors performed on two separate occasions are shown. Statistical tests: unpaired t- tests with Bonferroni corrections, each comparing against MOI 0 for the specific cell line. Statistics with * P < 0.05; ** P < 0.01. d Immunofluorescence micrographs showing CD3+TCRVα7.2+ MAIT cell (white arrow) within healthy human lung tissue (top panel) and 24 h post infection (bottom panels) ex vivo with L. longbeachae . Yellow arrow: intracellular L. longbechae bacilli. Red, TCRVα7.2; green, CD3; magenta, polyclonal rabbit anti -L. longbeachae ; blue, nuclei (Hoechst)
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    Human MAIT cells are activated by Legionella infection via MR1 in vitro. a Jurkat.MAIT and C1R.MR1 cells were co-incubated for 16 h with lysates of L. pneumophila (L. pn.) or L. longbeachae or 5-OP-RU, acetyl-6-formylpterin (Ac-6-FP) or PBS. Activation, detected by staining with anti-CD69, is enhanced by bacterial lysate or by the activating ligand 5-OP-RU, but not by acetyl-6-FP. Activation was blocked by anti-MR1 antibody (26.5) but not by isotype control (W6/32) 2 h prior to co-incubation. Experiment performed in triplicate wells on two separate occasions with similar results. Data show mean fluorescence intensity, MFI (±SEM). Statistical tests: one-way ANOVA and post hoc Dunnett’s comparing all columns with the first column (black). Unpaired t -test (blue), with *** P < 0.001; **** P < 0.0001. b , c THP1 cells (WT) or THP1 cells overexpressing MR1 (THP1.MR1+, purple) or deficient in expression of MR1 (THP1.MR1−, blue) were infected for 27 h with live or heat-killed (HK) L. longbeachae (MOI: 100) or 10 nM 5-OP-RU, then co-cultured for 16 h with sorted CD3 + Vα7.2 + CD161 + human peripheral blood MAIT cells, or MAIT-depleted conventional T cells. MR1-5-OP-RU-tetramer+ MAIT cell activation was measured by intracellular cytokine staining for b TNF or c IFN-γ. b , c Percentage cytokine-positive cells as mean (±SEM) data from three independent donors performed on two separate occasions are shown. Statistical tests: unpaired t- tests with Bonferroni corrections, each comparing against MOI 0 for the specific cell line. Statistics with * P < 0.05; ** P < 0.01. d Immunofluorescence micrographs showing CD3+TCRVα7.2+ MAIT cell (white arrow) within healthy human lung tissue (top panel) and 24 h post infection (bottom panels) ex vivo with L. longbeachae . Yellow arrow: intracellular L. longbechae bacilli. Red, TCRVα7.2; green, CD3; magenta, polyclonal rabbit anti -L. longbeachae ; blue, nuclei (Hoechst)

    Journal: Nature Communications

    Article Title: MAIT cells protect against pulmonary Legionella longbeachae infection

    doi: 10.1038/s41467-018-05202-8

    Figure Lengend Snippet: Human MAIT cells are activated by Legionella infection via MR1 in vitro. a Jurkat.MAIT and C1R.MR1 cells were co-incubated for 16 h with lysates of L. pneumophila (L. pn.) or L. longbeachae or 5-OP-RU, acetyl-6-formylpterin (Ac-6-FP) or PBS. Activation, detected by staining with anti-CD69, is enhanced by bacterial lysate or by the activating ligand 5-OP-RU, but not by acetyl-6-FP. Activation was blocked by anti-MR1 antibody (26.5) but not by isotype control (W6/32) 2 h prior to co-incubation. Experiment performed in triplicate wells on two separate occasions with similar results. Data show mean fluorescence intensity, MFI (±SEM). Statistical tests: one-way ANOVA and post hoc Dunnett’s comparing all columns with the first column (black). Unpaired t -test (blue), with *** P < 0.001; **** P < 0.0001. b , c THP1 cells (WT) or THP1 cells overexpressing MR1 (THP1.MR1+, purple) or deficient in expression of MR1 (THP1.MR1−, blue) were infected for 27 h with live or heat-killed (HK) L. longbeachae (MOI: 100) or 10 nM 5-OP-RU, then co-cultured for 16 h with sorted CD3 + Vα7.2 + CD161 + human peripheral blood MAIT cells, or MAIT-depleted conventional T cells. MR1-5-OP-RU-tetramer+ MAIT cell activation was measured by intracellular cytokine staining for b TNF or c IFN-γ. b , c Percentage cytokine-positive cells as mean (±SEM) data from three independent donors performed on two separate occasions are shown. Statistical tests: unpaired t- tests with Bonferroni corrections, each comparing against MOI 0 for the specific cell line. Statistics with * P < 0.05; ** P < 0.01. d Immunofluorescence micrographs showing CD3+TCRVα7.2+ MAIT cell (white arrow) within healthy human lung tissue (top panel) and 24 h post infection (bottom panels) ex vivo with L. longbeachae . Yellow arrow: intracellular L. longbechae bacilli. Red, TCRVα7.2; green, CD3; magenta, polyclonal rabbit anti -L. longbeachae ; blue, nuclei (Hoechst)

    Article Snippet: Supernatants were used to transduce parent THP1 cells (ATCC TIB-202, ATCC) and cells were selected using puromycin resistance and single-cell sorting using anti-MR1 antibody (8F2.F9) after upregulation of MR1 using acetyl-6-FP.

    Techniques: Infection, In Vitro, Incubation, Activation Assay, Staining, Control, Fluorescence, Expressing, Cell Culture, Immunofluorescence, Ex Vivo