panc 1  (ATCC)

Journal: Materials Today Bio

Article Title: Stromal homeostasis-restoring “rocket-like” nanomedicine inhibited pancreatic tumor growth in vivo

doi: 10.1016/j.mtbio.2026.103014

Figure Lengend Snippet: In vitro evaluation of Panc-1 and Pan02 cells after different treatments. (A) Colony formation assay of Panc-1 and Pan02 cells after different treatments. (B) Quantification of colony numbers of Panc-1 and Pan02 cells under the indicated treatments. (C) Representative images of cell migration of Panc-1 and Pan02 cells after different treatments. (D) Quantification of residual area of Panc-1 and Pan02 cells in each group. (E) ROS fluorescence intensity of Panc-1 cells after different treatments. (F) ROS fluorescence intensity of Pan02 cells after different treatments. (G) Viability of Panc-1 cells co-cultured with L929 cells in a transwell system after different treatments. (H) Viability of Pan02 cells co-cultured with L929 cells in a transwell system after different treatments. (I) Representative CLSM images of Panc-1 cells co-stained with Calcein-AM (green) and PI (red) after treatment with different groups (scale bar: 200 μm). (J) Representative CLSM images of Pan02 cells co-stained with Calcein-AM (green) and PI (red) after treatment with different groups. (K) Immunofluorescence staining of uPA in Panc-1 cells after different treatments.(scale bar:100 μm). (L) Immunofluorescence staining of uPA in Pan02 cells after different treatments. Data are presented as mean ± standard deviation (SD), n = 3. Statistical significance was analyzed by one-way ANOVA with t -test; ns, not significant; ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Triethylamine (TEA, Sigma-Aldrich); Cetyltrimethylammonium bromide (CTAB, Xinyanbomei); Sodium salicylate (NaSal, Sigma-Aldrich); Tetraethyl orthosilicate (TEOS, CATO); 1,2-Bis(triethoxysilyl)ethane (BTES, Xinhengyan); Ethanol; Hydrochloric acid; Methanol; Gemcitabine (MedChemExpress); Ammonium bicarbonate (Coolaber); Urokinase-type plasminogen activator (Solarbio); CCK-8 kit (CWBIO); ROS staining kit (Poolyue); Calcein-AM/PI kit (DOJINDO); anti-uPA antibody (HUABIO); Calcium chloride (Supelco); Indocyanine green (zrbiorise); Dulbecco's modified Eagle medium (DMEM, Sigma-Aldrich); Panc-1, Pan02, and L929 cells (ATCC); Fluorescein isothiocyanate (FITC, Qisong); 4′,6-diamidino-2-phenylindole (DAPI, Solarbio); DUTP (Roche); IPR-803 (MCE).

Techniques: In Vitro, Colony Assay, Migration, Fluorescence, Cell Culture, Staining, Immunofluorescence, Standard Deviation

Journal: bioRxiv

Article Title: The abscopal effect of IRE combined with anti–PD-1 achieves local ablation and systemic control of PDAC

doi: 10.64898/2026.05.04.722535

Figure Lengend Snippet: ( A ) Mitochondrial oxygen consumption rate (OCR) and ( B ) extracellular acidification rate (ECAR) in KRAS* cells 4 h after 10p IRE or 20p IRE (400 V). ( C ) Effect of IRE on mitochondrial ATP production. Data are normalized to cell number at the end of the assay (pmol/min/1000 cells). Data are expressed as mean ± SD (n=6/group). *p<0.05, **p<0.01, ***p<0.001 (one-way ANOVA). ( D ) Representative confocal fluorescence microscopic images of cytoplasmic dsDNA and corresponding quantification of fluorescence (FL) signal intensity in cytosol and nuclei. PANC-1 cells were stained with PicoGreen for dsDNA 24 h after IRE (400 V, 20 pulses). Untreated cells were used as a control. The negative control was cells not stained with PicoGreen. Data are expressed as mean ± SD (n=4). *p<0.05, *p<0.01, (Student’s t test). ( E ) Analysis of dsDNA from IRE-treated KRAS* tumors using the Qubit dsDNA HS assay kits. KRAS* tumor tissues were collected on day 1 and day 4 after IRE (1200 V, 99 pulses) and processed for dsDNA assays. *p<0.05, ****p<0.0001 (Student’s t test). ( F ) Real-time PCR analysis of the interferon-stimulated genes Irf1 , Cxcl10 , and Isg15 downstream of the cGAS-STING pathway. KRAS* cells were collected 4 h after IRE (400 V, 20 pulses). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (one-way ANOVA). Data are expressed as mean ± SD (n=3/group).

Article Snippet: The human pancreatic cancer cell line PANC-1 was purchased from American Type Culture Collection.

Techniques: Fluorescence, Staining, Control, Negative Control, Real-time Polymerase Chain Reaction