palter-1 vector (Promega)
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palter-1 vector
Palter 1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/palter-1+vector/palter+vector/pmc03126765-70-20-22
Average 90 stars, based on 1 article reviews
Palter 1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/palter-1+vector/palter+vector/pmc03126765-70-20-22
Average 90 stars, based on 1 article reviews
palter-1 vector - by Bioz Stars,
2026-09
90/100 stars
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Mutagenesis:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Plasmid Preparation:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Sequencing:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Binding Assay:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Clone Assay:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Polymerase Chain Reaction:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Generated:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the Construct:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the In Vitro:Article Title: Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter. Article Snippet: Morimoto K, Gosselink J, Kartono A, Hogg JC, Hayashi S, Ogawa E. Adenovirus E1A regulates lung epithelial ICAM-1 expression by interacting with transcriptional regulators at its promoter.. Am J Physiol Lung Cell Mol Physiol 296: L361–L371, 2009.. First published December 26, 2008; doi:10.1152/ajplung.90331.2008.—We focused on the regulation of inflammatory mediator expression by adenovirus E1A in lung epithelial cells and the role of this viral protein in the pathogenesis of chronic obstructive pulmonary disease (COPD). Article Title: Proposed structural mechanism of Escherichia coli cAMP receptor protein cAMP-dependent proteolytic cleavage protection and selective and nonselective DNA binding. Article Snippet: The Escherichia coli cAMP receptor protein (CRP) displays biphasic characteristics in protease and â-galactosidase induction assays at increasing cAMP concentrations in response to ligand binding at the secondary binding site located between the primary binding site and the DNA binding domain.. Two mutants were created to determine the mechanistic reason for the CRP biphasic response by inhibiting binding of cAMP to the secondary site via interference with the Arg 181 interaction with the ligand’s phosphate.. The S179A/R180D/E181H mutant binds two cAMP molecules per dimer, does not exhibit a biphasic response, lacks selective DNA binding, and has inhibited nonselective DNA binding. Article Title: Cell viability and secretion of active proteins in Schizosaccharomyces pombe do not require the chaperone function of calnexin Article Snippet: Plasmid pSPCA3262 (∆gly cnx1) was constructed by site- directed mutagenesis using the Altered Sites II in vitro Mutagenesis System and the Article Title: Molecular determinants of cAMP-mediated regulation of the Na+-Ca2+ exchanger expressed in human cell lines Article Snippet: The sarcolemmal Na–Ca family of proteins (NCX) appear to have evolved throughout the biosphere, from cyanobacteria and yeasts to humans, with the function of removing Ca from the cytosol in exchange for Na (Reuter & Seitz, 1968; Baker et al. 1969).. This electrogenic transport process is driven exclusively by the electrochemical gradients of Na and Ca, which also appear to allosterically regulate intracellular Ca-sensing sites for activation (DiPolo, 1979), and Na sites for inactivation of the exchanger (Hilgemann, 1990).. While this regulatory framework is generally conserved among species, other aspects of regulation of NCX appear to vary significantly from species to species or within an individual species, where functional diversity of NCX is produced by multiple genes (NCX1, NCX2, NCX3), each of which is expressed as tissue-specific splice variants (Quednau et al. 1997). Article Title: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Article Snippet: A mutation creating E 70 K as well as a Dra I restriction enzyme cleavage site was introduced into a Article Title: Role of Nuclear Factor Y in Stress-Induced Activation of the Herpes Simplex Virus Type 1 ICP0 Promoter Article Snippet: To clone the fragment containing the ICP0 promoter into the Article Title: Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex Article Snippet: The MED6 gene harboring own promoter and terminator regions was obtained from pRS316-MED6 by EcoRI-XbaI digestion and subcloned into the |
