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pact2 prey vector  (TaKaRa)


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    Structured Review

    TaKaRa pact2 prey vector
    Pact2 Prey Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pact2+prey+vector/pm38763272-45-12-15
    Average 86 stars, based on 1 article reviews
    pact2 prey vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Construct:

    Article Title: Regulation of gene expression during M-G1-phase in fission yeast through Plo1p and forkhead transcription factors.
    Article Snippet: .. To create the other two-hybrid constructs, cDNAs or genomic clones of fkh2+, mbx1+ and sep1+ were ligated into the pACT2 prey vector (Clontech). .. All constructs were confirmed by sequencing (MWG Biotech).

    Article Title: G protein β subunits regulate Ca v 3.3 T-type channel activity and current kinetics via interaction with the Ca v 3.3 C-terminus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: DYC-1, a Protein Functionally Linked to Dystrophin in Caenorhabditis elegans Is Associated with the Dense Body, Where It Interacts with the Muscle LIM Domain Protein ZYX-1
    Article Snippet: The resulting plasmid called pKG24 therefore encodes a fusion protein of gal4-BD and DYC-1S (aa 9-258 Δaa 54-81). .. Three constructs encoding fusion proteins composed of the gal4-AD-domain and different parts of the ZYX-1 protein were obtained by subcloning the corresponding zyx-1 cDNA fragments (cDNA clone yk247e1) into pACT2 prey vector (Clontech). ..

    Clone Assay:

    Article Title: Regulation of gene expression during M-G1-phase in fission yeast through Plo1p and forkhead transcription factors.
    Article Snippet: .. To create the other two-hybrid constructs, cDNAs or genomic clones of fkh2+, mbx1+ and sep1+ were ligated into the pACT2 prey vector (Clontech). .. All constructs were confirmed by sequencing (MWG Biotech).

    Article Title: Elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8.
    Article Snippet: The formation and function of cilia involves the movement of intraflagellar transport (IFT) particles underneath the ciliary membrane, along axonemal microtubules1,2.. Although this process has been studied extensively, its molecular basis remains incompletely understood.. For example, it is unknown how the IFT particle interacts with transmembrane proteins.

    Article Title: RUTBC2 Protein, a Rab9A Effector and GTPase-activating Protein for Rab36
    Article Snippet: Briefly, 56 mutant Rab proteins deficient for GTP hydrolysis (Gln to Ala) were cloned into pGBT9 bait vector (Clontech). .. RUTBC2 was amplified from cDNA libraries and was cloned into pACT2 prey vector (Clontech); growth on selective medium indicated an interaction between a Rab and RUTBC2. .. Plasmids For mammalian expression, N-terminally 3×Myc-tagged RUTBC2 was obtained by amplification from a cDNA library and ligation into a modified version of pCDNA3.1(+) (Invitrogen) ( 14 ).

    Plasmid Preparation:

    Article Title: Regulation of gene expression during M-G1-phase in fission yeast through Plo1p and forkhead transcription factors.
    Article Snippet: .. To create the other two-hybrid constructs, cDNAs or genomic clones of fkh2+, mbx1+ and sep1+ were ligated into the pACT2 prey vector (Clontech). .. All constructs were confirmed by sequencing (MWG Biotech).

    Article Title: Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity
    Article Snippet: .. For yeast two-hybrid screen and assays, the PDZ2 domain of PSD-95 (aa 89-299) in the pBHA bait vector was used to screen a human brain yeast two-hybrid cDNA library contained in the pACT2 prey vector (Clontech). ..

    Article Title: G protein β subunits regulate Ca v 3.3 T-type channel activity and current kinetics via interaction with the Ca v 3.3 C-terminus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8.
    Article Snippet: The formation and function of cilia involves the movement of intraflagellar transport (IFT) particles underneath the ciliary membrane, along axonemal microtubules1,2.. Although this process has been studied extensively, its molecular basis remains incompletely understood.. For example, it is unknown how the IFT particle interacts with transmembrane proteins.

    Article Title: RUTBC2 Protein, a Rab9A Effector and GTPase-activating Protein for Rab36
    Article Snippet: Briefly, 56 mutant Rab proteins deficient for GTP hydrolysis (Gln to Ala) were cloned into pGBT9 bait vector (Clontech). .. RUTBC2 was amplified from cDNA libraries and was cloned into pACT2 prey vector (Clontech); growth on selective medium indicated an interaction between a Rab and RUTBC2. .. Plasmids For mammalian expression, N-terminally 3×Myc-tagged RUTBC2 was obtained by amplification from a cDNA library and ligation into a modified version of pCDNA3.1(+) (Invitrogen) ( 14 ).

    Article Title: DYC-1, a Protein Functionally Linked to Dystrophin in Caenorhabditis elegans Is Associated with the Dense Body, Where It Interacts with the Muscle LIM Domain Protein ZYX-1
    Article Snippet: The resulting plasmid called pKG24 therefore encodes a fusion protein of gal4-BD and DYC-1S (aa 9-258 Δaa 54-81). .. Three constructs encoding fusion proteins composed of the gal4-AD-domain and different parts of the ZYX-1 protein were obtained by subcloning the corresponding zyx-1 cDNA fragments (cDNA clone yk247e1) into pACT2 prey vector (Clontech). ..

    Two Hybrid Screening:

    Article Title: Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity
    Article Snippet: .. For yeast two-hybrid screen and assays, the PDZ2 domain of PSD-95 (aa 89-299) in the pBHA bait vector was used to screen a human brain yeast two-hybrid cDNA library contained in the pACT2 prey vector (Clontech). ..

    cDNA Library Assay:

    Article Title: Synaptic adhesion molecule IgSF11 regulates synaptic transmission and plasticity
    Article Snippet: .. For yeast two-hybrid screen and assays, the PDZ2 domain of PSD-95 (aa 89-299) in the pBHA bait vector was used to screen a human brain yeast two-hybrid cDNA library contained in the pACT2 prey vector (Clontech). ..

    Article Title: G protein β subunits regulate Ca v 3.3 T-type channel activity and current kinetics via interaction with the Ca v 3.3 C-terminus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Sequencing:

    Article Title: Idiosyncratic Helix-Turn-Helix Motif in Methanosarcina barkeri Seryl-tRNA Synthetase Has a Critical Architectural Role
    Article Snippet: .. Yeast Two-hybrid Analysis—The N-terminal domain was recloned from the pET15b-mMbSerRS-NTD vector as an NcoI/ XhoI cassette in-frame to the 3 -end of the coding sequence of the transcription factor Gal4 activation domain of the pACT2 prey vector (Clontech,Mountain View, CA). .. C-terminal domains with or without the HTH motif were recloned from pET28b- mMbSerRS-CTDandpET28b-mMbSerRS-CTD HTHplasmids in-frame to the 3 -end of the coding sequence of the transcription factor LexA DNA binding domain (LexA BD) of bait plasmid pAB151 (14)asNdeI/SmaIcassettes.

    Article Title: Idiosyncratic Helix-Turn-Helix Motif in Methanosarcina barkeri Seryl-tRNA Synthetase Has a Critical Architectural Role
    Article Snippet: .. Yeast Two-hybrid Analysis —The N-terminal domain was recloned from the pET15b-mMbSerRS-NTD vector as an NcoI/XhoI cassette in-frame to the 3′-end of the coding sequence of the transcription factor Gal4 activation domain of the pACT2 prey vector (Clontech, Mountain View, CA). .. C-terminal domains with or without the HTH motif were recloned from pET28b-mMbSerRS-CTD and pET28b-mMbSerRS-CTDΔHTH plasmids in-frame to the 3′-end of the coding sequence of the transcription factor LexA DNA binding domain (LexA BD) of bait plasmid pAB151 ( 14 ) as NdeI/SmaI cassettes.

    Activation Assay:

    Article Title: Idiosyncratic Helix-Turn-Helix Motif in Methanosarcina barkeri Seryl-tRNA Synthetase Has a Critical Architectural Role
    Article Snippet: .. Yeast Two-hybrid Analysis—The N-terminal domain was recloned from the pET15b-mMbSerRS-NTD vector as an NcoI/ XhoI cassette in-frame to the 3 -end of the coding sequence of the transcription factor Gal4 activation domain of the pACT2 prey vector (Clontech,Mountain View, CA). .. C-terminal domains with or without the HTH motif were recloned from pET28b- mMbSerRS-CTDandpET28b-mMbSerRS-CTD HTHplasmids in-frame to the 3 -end of the coding sequence of the transcription factor LexA DNA binding domain (LexA BD) of bait plasmid pAB151 (14)asNdeI/SmaIcassettes.

    Article Title: Idiosyncratic Helix-Turn-Helix Motif in Methanosarcina barkeri Seryl-tRNA Synthetase Has a Critical Architectural Role
    Article Snippet: .. Yeast Two-hybrid Analysis —The N-terminal domain was recloned from the pET15b-mMbSerRS-NTD vector as an NcoI/XhoI cassette in-frame to the 3′-end of the coding sequence of the transcription factor Gal4 activation domain of the pACT2 prey vector (Clontech, Mountain View, CA). .. C-terminal domains with or without the HTH motif were recloned from pET28b-mMbSerRS-CTD and pET28b-mMbSerRS-CTDΔHTH plasmids in-frame to the 3′-end of the coding sequence of the transcription factor LexA DNA binding domain (LexA BD) of bait plasmid pAB151 ( 14 ) as NdeI/SmaI cassettes.

    Yeast:

    Article Title: G protein β subunits regulate Ca v 3.3 T-type channel activity and current kinetics via interaction with the Ca v 3.3 C-terminus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Amplification:

    Article Title: RUTBC2 Protein, a Rab9A Effector and GTPase-activating Protein for Rab36
    Article Snippet: Briefly, 56 mutant Rab proteins deficient for GTP hydrolysis (Gln to Ala) were cloned into pGBT9 bait vector (Clontech). .. RUTBC2 was amplified from cDNA libraries and was cloned into pACT2 prey vector (Clontech); growth on selective medium indicated an interaction between a Rab and RUTBC2. .. Plasmids For mammalian expression, N-terminally 3×Myc-tagged RUTBC2 was obtained by amplification from a cDNA library and ligation into a modified version of pCDNA3.1(+) (Invitrogen) ( 14 ).

    Subcloning:

    Article Title: DYC-1, a Protein Functionally Linked to Dystrophin in Caenorhabditis elegans Is Associated with the Dense Body, Where It Interacts with the Muscle LIM Domain Protein ZYX-1
    Article Snippet: The resulting plasmid called pKG24 therefore encodes a fusion protein of gal4-BD and DYC-1S (aa 9-258 Δaa 54-81). .. Three constructs encoding fusion proteins composed of the gal4-AD-domain and different parts of the ZYX-1 protein were obtained by subcloning the corresponding zyx-1 cDNA fragments (cDNA clone yk247e1) into pACT2 prey vector (Clontech). ..



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    Image Search Results


    Plasmids used in this study

    Journal: The Journal of Biological Chemistry

    Article Title: N-terminal methionine excision of proteins creates tertiary destabilizing N-degrons of the Arg/N-end rule pathway

    doi: 10.1074/jbc.RA118.006913

    Figure Lengend Snippet: Plasmids used in this study

    Article Snippet: Y2H screening and binding assays To screen human UBR1-binding proteins, a human pancreas cDNA library cloned into the prey pACT2 vector (638820, Clontech) was transformed into S. cerevisiae strain pJ69 l4A expressing a bait pCH1591 (expressing human UBR1 1–1031 ) using the lithium-acetate protocol ( 61 ).

    Techniques: Plasmid Preparation, Marker