packagene lambda dna packaging system (Promega)
90
Structured Review
Promega
packagene lambda dna packaging system
Packagene Lambda Dna Packaging System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/packagene%C2%AE+lambda+dna+packaging+system/packagene+lambda+dna+packaging+system/pmc05854184-315-16-22
Average 90 stars, based on 1 article reviews
Packagene Lambda Dna Packaging System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/packagene%C2%AE+lambda+dna+packaging+system/packagene+lambda+dna+packaging+system/pmc05854184-315-16-22
Average 90 stars, based on 1 article reviews
packagene lambda dna packaging system - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases"
Article Title: Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases
Journal: Methods in enzymology
doi: 10.1016/bs.mie.2016.09.010
Figure Legend Snippet: Visualizing unwinding of individual DNA molecules using fluorescent SSB protein and TIRF microscopy. (A) Top: Diagram of phage λ DNA molecule with the indicated number of biotin groups incorporated in each 12-nt cos overhang. Middle: Illustration of biotinylated λ DNA attached at both ends via biotin–streptavidin linkage. Bottom: Image of an actual λ DNA molecule attached to the glass surface, stained with YO-PRO-1 (100 nM), and illuminated with a 488 nm laser. The image is false colored in green and the attachment points to the glass surface are indicated. (B) The process required to construct a flow cell containing three separate single-channels. The steps highlighted are equivalent to those described in Section 4.1. (C) The flow cell from (B) mounted onto the objective; biotinylated lambda DNA was injected under buffer flow, permitting attachment of both ends to the surface. Unwinding tracks are visualized by binding of AF488-SSBG26C (green) to ssDNA regions. (D) Schematic representation of a TIRF microscope capable of visualizing DNA unwinding by RecQ by monitoring signal from both DNA and fluorescent SSB simultaneously. As shown in (C) the flow cell is mounted onto a 100× oil-immersion objective. The fluorescent SSB and DNA are excited by two lasers; 488 and 561 nm, respectively, and emission measured, via dichroic mirrors (M1 and M2). The deconvoluted emission is then directed onto different areas of a CCD camera generating a signal corresponding to either SSB or DNA. The lasers are operated using a custom LABview VI program to coordinate excitation with image acquisition so that the sample is illuminated only during the exposure times. Panels (A) and (D): From Rad, B., Forget, A. L., Baskin, R. J., & Kowalczykowski, S. C. (2015). Single-molecule visualization of RecQ helicase reveals DNA melting, nucleation, and assembly are required for processive DNA unwinding. Proceedings of the National Academy of Sciences of the United States of America, 112(50), E6852–E6861. http://dx.doi.org/10.1073/pnas.1518028112.
Techniques Used: Microscopy, Staining, Construct, Lambda DNA Preparation, Injection, Binding Assay
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Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the cDNA Library Assay:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Construct:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the In Vitro:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Cloning:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Isolation:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Reverse Transcription:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Expressing:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Polymerase Chain Reaction:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the Amplification:Article Title: Cloning and structural analysis of two highly divergent IgA isotypes, IgA1 and IgA2 from the duck billed platypus, Ornithorhynchus anatinus. Article Snippet: To trace the emergence of modern IgA isotypes during vertebrate evolution we have studied the immunoglobulin repertoire of a model monotreme, the platypus.. Two highly divergent IgA-like isotypes (IgA1 and IgA2) were identified and their primary structures were determined from full-length cDNAs.. A comparative analysis of the amino acid sequences for IgA from various animal species showed that the two platypus IgA isotypes form a branch clearly separated from their eutherian (placental) counterparts. Article Title: Methods and compositions for recombination a gene-deficient strains of Article Snippet: The ligation mixture is processed using Promega's Article Title: COD URACIL-DNA GLYCOSYLASE, GENE CODING THEREFORE, RECOMBINANT DNA CONTAINING SAID GENE OR OPERATIVE PARTS THEREOF, A METHOD FOR PREPARING SAID PROTEIN AND THE USE OF SAID PROTEIN OR SAID OPERATIVE PARTS THEREOF IN MONITORING OR CONTROLLING PCR Article Snippet: Isolation of the Uracil-DNA Glycosylase Gene from Gadus morhua The following materials were used; SuperScriptTM II Rnase H− Reverse Transcriptase (Gibco BRL), Article Title: pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Article Snippet: A cosmid cloning vector has been constructed that demonstrates high levels of segregational stability in Escherichia coli K12. pPSX is a 14-kilobase vector derived from the IncW plasmid pR388. pPSX is highly stable in E. coli in the absence of antibiotic selection, even while expressing the toxic indolocarbazole antitumor antibiotic violacein.. The incorporation of the cos sequence enables construction of cosmid libraries with inserts ranging from 24 to 36 kb.. The inclusion of a lacZ multiple cloning site (MCS) allows blue/white screening. pPSX cosmids can be extracted from the host cell with commercial plasmid extraction kits facilitating downstream analysis, sequencing and sub-cloning. pPSX can be transferred to a variety of heterologous hosts by either electroporation or mobilization from E. coli S17-1. Article Title: Human Cord Blood Derived Immature Basophils Show Dual Characteristics, Expressing Both Basophil and Eosinophil Associated Proteins Article Snippet: Subsequently, double stranded cDNA was synthesized using the TimeSaver cDNA Synthesis Kit (Amersham Pharmacia Biotech, Uppsala, Sweden). Article Title: Apple FLOWERING LOCUS T proteins interact with transcription factors implicated in cell growth and organ development. Article Snippet: Screening an apple cDNA library To isolate the full-length cDNAs of apple TCP and VOZ1 homologues MdTCP2, MdTCP4 and MdVOZ1, we screened an apple cDNA library that was constructed using a ZAP-cDNA Synthesis Kit (Stratagene) and a Article Title: Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof Article Snippet: The ligation mixture was then encapsidated in vitro in lambda phage particles using the Article Title: Mycobacterial MazG Is a Novel NTP Pyrophosphohydrolase Involved in Oxidative Stress Response Article Snippet: The resulting cosmid was digested by PacI and inserted into the genome of mycobacteriophage phAE87 and was subsequently packaged by the |