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micro bio spin p6 gel columns  (Bio-Rad)


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    Bio-Rad micro bio spin p6 gel columns
    Micro Bio Spin P6 Gel Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-6+columns/Micro+Bio-Spin+P-6+Gel+Columns/pm42104933-197-11-16
    Average 96 stars, based on 642 article reviews
    micro bio spin p6 gel columns - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation.
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc)2) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Article Title: Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes.
    Article Snippet: Journal of Controlled Release 149 (2011) 146–158 Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l G E N E D E L IV E R Y Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes Sabrina Höbel a, Andrea Loos b, Dietmar Appelhans b, Simona Schwarz b, Jürgen Seidel c, Brigitte Voit b, Achim Aigner a,⁎ a Pharmacological Institute, Philipps-University, Faculty of Medicine, Karl-v.-Frisch-Strasse 1, D-35033 Marburg, Germany b Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069 Dresden, Germany c Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Strasse 29, D-09596 Freiberg, Germany Abbreviations: PEI, polyethylenimine; OM-PEI, oligo small interfering RNA.. ⁎ Corresponding author.. Tel.

    Article Title: Modified tumor necrosis factor-alpha converting enzyme and methods of use thereof
    Article Snippet: Stability of TACE and TACE mutants: To test for stability under different storage conditions, 20 ul of TACE protein, at 15 mg/ml, was desalted over BIO-RAD P-6 columns equilibrated at pH 7.5 in: (a) 25 mM Tris, 0.15M NaCl; (b) 25 mM Tris+1 mM N-{D,L-2-(hydroxyaminocarbonyl)methyl-4-methylpentanoyl}-L-3-amino-2-dimethylbutanoyl-L-alanine, 2-(amino)ethyl amide; or (c) 25 mM Tris.

    Article Title: A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.
    Article Snippet: In total, 100 mL of 10 mM oligonucleotide solution in nuclease-free water was treated with 4 mL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (BioRad).

    Article Title: Therapeutic Monoclonal Antibody Intact Mass Analysis by Capillary Electrophoresis–Mass Spectrometry
    Article Snippet: The characterization of monoclonal antibody (mAb) therapeutics via mass spectroscopy is of important value in determining sequence integrity and identifying post-translational modifications.. The monoclonal antibodies are commonly either reduced to generate heavy chain and light chain, or enzymatically cleaved to produce characteristic domains for subunit intact mass analysis.. Toward this end, liquid chromatography coupled with mass spectrometry (LC-MS) is usually applied for the separation of these antibody subunits followed by online mass analysis [1–3].

    Article Title: A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus
    Article Snippet: In total, 100 μL of 10 μM oligonucleotide solution in nuclease-free water was treated with 4 μL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (Bio-Rad).

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc) 2 ) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Purification:

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation.
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc)2) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Article Title: Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes.
    Article Snippet: Journal of Controlled Release 149 (2011) 146–158 Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l G E N E D E L IV E R Y Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes Sabrina Höbel a, Andrea Loos b, Dietmar Appelhans b, Simona Schwarz b, Jürgen Seidel c, Brigitte Voit b, Achim Aigner a,⁎ a Pharmacological Institute, Philipps-University, Faculty of Medicine, Karl-v.-Frisch-Strasse 1, D-35033 Marburg, Germany b Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069 Dresden, Germany c Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Strasse 29, D-09596 Freiberg, Germany Abbreviations: PEI, polyethylenimine; OM-PEI, oligo small interfering RNA.. ⁎ Corresponding author.. Tel.

    Article Title: Modified tumor necrosis factor-alpha converting enzyme and methods of use thereof
    Article Snippet: Stability of TACE and TACE mutants: To test for stability under different storage conditions, 20 ul of TACE protein, at 15 mg/ml, was desalted over BIO-RAD P-6 columns equilibrated at pH 7.5 in: (a) 25 mM Tris, 0.15M NaCl; (b) 25 mM Tris+1 mM N-{D,L-2-(hydroxyaminocarbonyl)methyl-4-methylpentanoyl}-L-3-amino-2-dimethylbutanoyl-L-alanine, 2-(amino)ethyl amide; or (c) 25 mM Tris.

    Article Title: A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.
    Article Snippet: In total, 100 mL of 10 mM oligonucleotide solution in nuclease-free water was treated with 4 mL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (BioRad).

    Article Title: Therapeutic Monoclonal Antibody Intact Mass Analysis by Capillary Electrophoresis–Mass Spectrometry
    Article Snippet: The characterization of monoclonal antibody (mAb) therapeutics via mass spectroscopy is of important value in determining sequence integrity and identifying post-translational modifications.. The monoclonal antibodies are commonly either reduced to generate heavy chain and light chain, or enzymatically cleaved to produce characteristic domains for subunit intact mass analysis.. Toward this end, liquid chromatography coupled with mass spectrometry (LC-MS) is usually applied for the separation of these antibody subunits followed by online mass analysis [1–3].

    Article Title: A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus
    Article Snippet: In total, 100 μL of 10 μM oligonucleotide solution in nuclease-free water was treated with 4 μL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (Bio-Rad).

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc) 2 ) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Hybridization:

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation.
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc)2) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Article Title: Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes.
    Article Snippet: Journal of Controlled Release 149 (2011) 146–158 Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l G E N E D E L IV E R Y Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes Sabrina Höbel a, Andrea Loos b, Dietmar Appelhans b, Simona Schwarz b, Jürgen Seidel c, Brigitte Voit b, Achim Aigner a,⁎ a Pharmacological Institute, Philipps-University, Faculty of Medicine, Karl-v.-Frisch-Strasse 1, D-35033 Marburg, Germany b Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069 Dresden, Germany c Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Strasse 29, D-09596 Freiberg, Germany Abbreviations: PEI, polyethylenimine; OM-PEI, oligo small interfering RNA.. ⁎ Corresponding author.. Tel.

    Article Title: Modified tumor necrosis factor-alpha converting enzyme and methods of use thereof
    Article Snippet: Stability of TACE and TACE mutants: To test for stability under different storage conditions, 20 ul of TACE protein, at 15 mg/ml, was desalted over BIO-RAD P-6 columns equilibrated at pH 7.5 in: (a) 25 mM Tris, 0.15M NaCl; (b) 25 mM Tris+1 mM N-{D,L-2-(hydroxyaminocarbonyl)methyl-4-methylpentanoyl}-L-3-amino-2-dimethylbutanoyl-L-alanine, 2-(amino)ethyl amide; or (c) 25 mM Tris.

    Article Title: A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.
    Article Snippet: In total, 100 mL of 10 mM oligonucleotide solution in nuclease-free water was treated with 4 mL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (BioRad).

    Article Title: Therapeutic Monoclonal Antibody Intact Mass Analysis by Capillary Electrophoresis–Mass Spectrometry
    Article Snippet: The characterization of monoclonal antibody (mAb) therapeutics via mass spectroscopy is of important value in determining sequence integrity and identifying post-translational modifications.. The monoclonal antibodies are commonly either reduced to generate heavy chain and light chain, or enzymatically cleaved to produce characteristic domains for subunit intact mass analysis.. Toward this end, liquid chromatography coupled with mass spectrometry (LC-MS) is usually applied for the separation of these antibody subunits followed by online mass analysis [1–3].

    Article Title: A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus
    Article Snippet: In total, 100 μL of 10 μM oligonucleotide solution in nuclease-free water was treated with 4 μL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (Bio-Rad).

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc) 2 ) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Chromatography:

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation.
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc)2) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Article Title: Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes.
    Article Snippet: Journal of Controlled Release 149 (2011) 146–158 Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l G E N E D E L IV E R Y Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes Sabrina Höbel a, Andrea Loos b, Dietmar Appelhans b, Simona Schwarz b, Jürgen Seidel c, Brigitte Voit b, Achim Aigner a,⁎ a Pharmacological Institute, Philipps-University, Faculty of Medicine, Karl-v.-Frisch-Strasse 1, D-35033 Marburg, Germany b Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069 Dresden, Germany c Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Strasse 29, D-09596 Freiberg, Germany Abbreviations: PEI, polyethylenimine; OM-PEI, oligo small interfering RNA.. ⁎ Corresponding author.. Tel.

    Article Title: Modified tumor necrosis factor-alpha converting enzyme and methods of use thereof
    Article Snippet: Stability of TACE and TACE mutants: To test for stability under different storage conditions, 20 ul of TACE protein, at 15 mg/ml, was desalted over BIO-RAD P-6 columns equilibrated at pH 7.5 in: (a) 25 mM Tris, 0.15M NaCl; (b) 25 mM Tris+1 mM N-{D,L-2-(hydroxyaminocarbonyl)methyl-4-methylpentanoyl}-L-3-amino-2-dimethylbutanoyl-L-alanine, 2-(amino)ethyl amide; or (c) 25 mM Tris.

    Article Title: A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.
    Article Snippet: In total, 100 mL of 10 mM oligonucleotide solution in nuclease-free water was treated with 4 mL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (BioRad).

    Article Title: Therapeutic Monoclonal Antibody Intact Mass Analysis by Capillary Electrophoresis–Mass Spectrometry
    Article Snippet: The characterization of monoclonal antibody (mAb) therapeutics via mass spectroscopy is of important value in determining sequence integrity and identifying post-translational modifications.. The monoclonal antibodies are commonly either reduced to generate heavy chain and light chain, or enzymatically cleaved to produce characteristic domains for subunit intact mass analysis.. Toward this end, liquid chromatography coupled with mass spectrometry (LC-MS) is usually applied for the separation of these antibody subunits followed by online mass analysis [1–3].

    Article Title: A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus
    Article Snippet: In total, 100 μL of 10 μM oligonucleotide solution in nuclease-free water was treated with 4 μL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (Bio-Rad).

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc) 2 ) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Centrifugation:

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation.
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc)2) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.

    Article Title: Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes.
    Article Snippet: Journal of Controlled Release 149 (2011) 146–158 Contents lists available at ScienceDirect Journal of Controlled Release j ourna l homepage: www.e lsev ie r.com/ locate / jconre l G E N E D E L IV E R Y Maltose- and maltotriose-modified, hyperbranched poly(ethylene imine)s (OM-PEIs): Physicochemical and biological properties of DNA and siRNA complexes Sabrina Höbel a, Andrea Loos b, Dietmar Appelhans b, Simona Schwarz b, Jürgen Seidel c, Brigitte Voit b, Achim Aigner a,⁎ a Pharmacological Institute, Philipps-University, Faculty of Medicine, Karl-v.-Frisch-Strasse 1, D-35033 Marburg, Germany b Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069 Dresden, Germany c Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Strasse 29, D-09596 Freiberg, Germany Abbreviations: PEI, polyethylenimine; OM-PEI, oligo small interfering RNA.. ⁎ Corresponding author.. Tel.

    Article Title: Modified tumor necrosis factor-alpha converting enzyme and methods of use thereof
    Article Snippet: Stability of TACE and TACE mutants: To test for stability under different storage conditions, 20 ul of TACE protein, at 15 mg/ml, was desalted over BIO-RAD P-6 columns equilibrated at pH 7.5 in: (a) 25 mM Tris, 0.15M NaCl; (b) 25 mM Tris+1 mM N-{D,L-2-(hydroxyaminocarbonyl)methyl-4-methylpentanoyl}-L-3-amino-2-dimethylbutanoyl-L-alanine, 2-(amino)ethyl amide; or (c) 25 mM Tris.

    Article Title: A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus.
    Article Snippet: In total, 100 mL of 10 mM oligonucleotide solution in nuclease-free water was treated with 4 mL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (BioRad).

    Article Title: Therapeutic Monoclonal Antibody Intact Mass Analysis by Capillary Electrophoresis–Mass Spectrometry
    Article Snippet: The characterization of monoclonal antibody (mAb) therapeutics via mass spectroscopy is of important value in determining sequence integrity and identifying post-translational modifications.. The monoclonal antibodies are commonly either reduced to generate heavy chain and light chain, or enzymatically cleaved to produce characteristic domains for subunit intact mass analysis.. Toward this end, liquid chromatography coupled with mass spectrometry (LC-MS) is usually applied for the separation of these antibody subunits followed by online mass analysis [1–3].

    Article Title: A New Restriction Endonuclease-Based Method for Highly-Specific Detection of DNA Targets from Methicillin-Resistant Staphylococcus aureus
    Article Snippet: In total, 100 μL of 10 μM oligonucleotide solution in nuclease-free water was treated with 4 μL of 500 mM DTT solution in water, and then incubated at room temperature for 3 h. The reaction mixture was purified from the excess of DTT by applying twice to P-6 columns (Bio-Rad).

    Article Title: Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation
    Article Snippet: The pellet was resuspended with tRNA storage buffer (10 mM NaOAc pH 5.2, 50 mM Mg(OAc) 2 ) and further purified by passing through BioRad P-6 columns that were equilibrated with tRNA storage buffer.



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