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p rb1  (Proteintech)


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    Proteintech p rb1
    P Rb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+rb1/RB1+Antibody/pm41202397-139-42-43
    Average 93 stars, based on 65 article reviews
    p rb1 - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Mechanisms underlying the therapeutic effects of Amygdalin in treating Cervical Cancer based on multi-omics analysis.
    Article Snippet: Protein samples were separated by 10 % SDSpolyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (EMD Millipore, Billerica, MA, USA). .. The membranes were incubated overnight at 4 °C with primary antibodies against human CA9 (Proteintech Group, Inc., Rosemont, IL, USA), HK2 (Proteintech Group, Inc., Rosemont, IL, USA), Cyclin D1 (Proteintech Group, Inc., Rosemont, IL, USA), RB1 (Proteintech Group, Inc., Rosemont, IL, USA), p-RB1(Ser780) (Proteintech Group, Inc., Rosemont, IL, USA), p-CDK1(Tyr15) (Proteintech Group, Inc., Rosemont, IL, USA), P21 (Proteintech Group, Inc., Rosemont, IL, USA), and GAPDH (Cell Signaling Technology, Danvers, MA, USA), followed by incubation with secondary antibodies (LI-COR, Inc., Lincoln, NE, USA) for 2 h at room temperature. .. Band visualization and subsequent quantification of protein density were performed using the Odyssey Classic infrared imaging system (LI-COR, Inc., Lincoln, NE, USA).



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    p rb1  (Proteintech)
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    In vivo functions of miR-182-5p and R-2HG in mice model. ( A-B ) Xenograft tumor model was established in nude mice and R-2HG and/or antagomir-182-5p injection was administered as described. Tumor appearance, weight, and volume were examined. Scale bar = 1 cm. ( C ) The miR-182-5p levels in tumor tissues were examined using qRT-PCR. ( D ) Pathological alterations of tumor tissues were evaluated using H&E staining. Scale bar = 100 μm. ( E ) The protein levels of CDKN2C, CDK4, Cyclin D1, <t>p-RB1,</t> <t>RB1,</t> Bax, and Bcl-2 in tumor tissues were examined using Immunoblotting. * p < 0.05, ** p < 0.01 compared with PBS + antagomir-NC; ## p < 0.01, compared with PBS + antagomir-182-5p
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    anti p rb1 ser807 811 antibody  (Cell Signaling Technology Inc)
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    ( A ) Heatmap analysis of a high-throughput cell cycle protein ELISA of ECs isolated from livers of P6 BMP9/10ib mice vs. PBS control littermates. Data represent n=2 independent analyses (n represents one litter of pups combined for each condition; PBS, n=6 mice; BMP9/10ib, n=7 mice). ( B ) qPCR analysis of ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=5/condition). Data represent mean ± SEM, Mann-Whitney test ( Cdk2 ), and unpaired t-test ( Cdk4 and Cdk6 ). ( C ) Flow cytometry quantification of <t>p-RB1</t> fluorescence intensity in ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=3/condition). Data represent mean ± SEM, unpaired t-test. *P < 0.05. ( D and E ) Representative IF staining of <t>p-RB1</t> (green) and IB4 (red) in the peri-optic nerve and mid-plexus regions ( D ) and corresponding quantification of the peri-optic nerve region ( E ) of retinas from vehicle-treated PBS (n=6), palbociclib-treated PBS (n=5), vehicle-treated BMP9/10ib (n=6), and palbociclib-treated BMP9/10ib (n=6) mice. Data in ( E ) represent individual retinas and mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test. ns, not significant; *P < 0.05, **P < 0.01. Scale bars in ( D ), 50 μm. ( F ) Representative H&E and IHC staining of p-RB1 in 4 μm skin sections of HHT2 patients. Te, telangiectasia; N, normal vessel; Ba, basilar cells.
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    anti p rb1 ser807 811  (Cell Signaling Technology Inc)
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    ( A ) Heatmap analysis of a high-throughput cell cycle protein ELISA of ECs isolated from livers of P6 BMP9/10ib mice vs. PBS control littermates. Data represent n=2 independent analyses (n represents one litter of pups combined for each condition; PBS, n=6 mice; BMP9/10ib, n=7 mice). ( B ) qPCR analysis of ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=5/condition). Data represent mean ± SEM, Mann-Whitney test ( Cdk2 ), and unpaired t-test ( Cdk4 and Cdk6 ). ( C ) Flow cytometry quantification of <t>p-RB1</t> fluorescence intensity in ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=3/condition). Data represent mean ± SEM, unpaired t-test. *P < 0.05. ( D and E ) Representative IF staining of <t>p-RB1</t> (green) and IB4 (red) in the peri-optic nerve and mid-plexus regions ( D ) and corresponding quantification of the peri-optic nerve region ( E ) of retinas from vehicle-treated PBS (n=6), palbociclib-treated PBS (n=5), vehicle-treated BMP9/10ib (n=6), and palbociclib-treated BMP9/10ib (n=6) mice. Data in ( E ) represent individual retinas and mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test. ns, not significant; *P < 0.05, **P < 0.01. Scale bars in ( D ), 50 μm. ( F ) Representative H&E and IHC staining of p-RB1 in 4 μm skin sections of HHT2 patients. Te, telangiectasia; N, normal vessel; Ba, basilar cells.
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    In vivo functions of miR-182-5p and R-2HG in mice model. ( A-B ) Xenograft tumor model was established in nude mice and R-2HG and/or antagomir-182-5p injection was administered as described. Tumor appearance, weight, and volume were examined. Scale bar = 1 cm. ( C ) The miR-182-5p levels in tumor tissues were examined using qRT-PCR. ( D ) Pathological alterations of tumor tissues were evaluated using H&E staining. Scale bar = 100 μm. ( E ) The protein levels of CDKN2C, CDK4, Cyclin D1, p-RB1, RB1, Bax, and Bcl-2 in tumor tissues were examined using Immunoblotting. * p < 0.05, ** p < 0.01 compared with PBS + antagomir-NC; ## p < 0.01, compared with PBS + antagomir-182-5p

    Journal: Biological Research

    Article Title: IDH1 mutation produces R-2-hydroxyglutarate (R-2HG) and induces mir-182-5p expression to regulate cell cycle and tumor formation in glioma

    doi: 10.1186/s40659-024-00512-2

    Figure Lengend Snippet: In vivo functions of miR-182-5p and R-2HG in mice model. ( A-B ) Xenograft tumor model was established in nude mice and R-2HG and/or antagomir-182-5p injection was administered as described. Tumor appearance, weight, and volume were examined. Scale bar = 1 cm. ( C ) The miR-182-5p levels in tumor tissues were examined using qRT-PCR. ( D ) Pathological alterations of tumor tissues were evaluated using H&E staining. Scale bar = 100 μm. ( E ) The protein levels of CDKN2C, CDK4, Cyclin D1, p-RB1, RB1, Bax, and Bcl-2 in tumor tissues were examined using Immunoblotting. * p < 0.05, ** p < 0.01 compared with PBS + antagomir-NC; ## p < 0.01, compared with PBS + antagomir-182-5p

    Article Snippet: After that, following electrophoresis by SDS-PAGE, the separated proteins (50 µg) were electroblotted from the gel on PVDF membranes (Sigma-Aldrich). followed by an overnight treatment at 4 °C with primary antibodies: CDKN2C (ab192239, Abcam, Cambridge, USA), Cyclin D1 (60186-1-Ig, Proteintech, Wuhan, China), RB1 (ab181616, Abcam), p-RB1 (ab184796, Abcam), CDK4 (11026-1-AP, Proteintech), Bcl-2 (12789-1-AP, Proteintech), BAX (50599-2-Ig, Proteintech), and then 1.5-h interaction with secondary antibody (Abcam) at room temperature (RT).

    Techniques: In Vivo, Injection, Quantitative RT-PCR, Staining, Western Blot

    Dynamic effects of the miR-182-5p/CDKN2C axis on R-2HG-induced malignant cell phenotypes. BT142 and U251-MG cells were co-transduced with antagomir-182-5p and small interfering RNA targeting CDKN2C (si-CDKN2C), treated with R-2HG, and examined for the protein levels of CDKN2C using Immunoblotting ( A ); cell viability using CCK-8 assay ( B ); cell apoptosis and cell cycle distribution using Flow cytometry ( C-D ); the protein levels of cyclin D1, p-RB1, RB1, Bax, and Bcl-2 using Immunoblotting ( E ). * p < 0.05, ** p < 0.01 compared with antagomir-NC + si-NC; ## p < 0.01, compared with antagomir-NC + si- CDKN2C

    Journal: Biological Research

    Article Title: IDH1 mutation produces R-2-hydroxyglutarate (R-2HG) and induces mir-182-5p expression to regulate cell cycle and tumor formation in glioma

    doi: 10.1186/s40659-024-00512-2

    Figure Lengend Snippet: Dynamic effects of the miR-182-5p/CDKN2C axis on R-2HG-induced malignant cell phenotypes. BT142 and U251-MG cells were co-transduced with antagomir-182-5p and small interfering RNA targeting CDKN2C (si-CDKN2C), treated with R-2HG, and examined for the protein levels of CDKN2C using Immunoblotting ( A ); cell viability using CCK-8 assay ( B ); cell apoptosis and cell cycle distribution using Flow cytometry ( C-D ); the protein levels of cyclin D1, p-RB1, RB1, Bax, and Bcl-2 using Immunoblotting ( E ). * p < 0.05, ** p < 0.01 compared with antagomir-NC + si-NC; ## p < 0.01, compared with antagomir-NC + si- CDKN2C

    Article Snippet: After that, following electrophoresis by SDS-PAGE, the separated proteins (50 µg) were electroblotted from the gel on PVDF membranes (Sigma-Aldrich). followed by an overnight treatment at 4 °C with primary antibodies: CDKN2C (ab192239, Abcam, Cambridge, USA), Cyclin D1 (60186-1-Ig, Proteintech, Wuhan, China), RB1 (ab181616, Abcam), p-RB1 (ab184796, Abcam), CDK4 (11026-1-AP, Proteintech), Bcl-2 (12789-1-AP, Proteintech), BAX (50599-2-Ig, Proteintech), and then 1.5-h interaction with secondary antibody (Abcam) at room temperature (RT).

    Techniques: Transduction, Small Interfering RNA, Western Blot, CCK-8 Assay, Flow Cytometry

    ( A ) Heatmap analysis of a high-throughput cell cycle protein ELISA of ECs isolated from livers of P6 BMP9/10ib mice vs. PBS control littermates. Data represent n=2 independent analyses (n represents one litter of pups combined for each condition; PBS, n=6 mice; BMP9/10ib, n=7 mice). ( B ) qPCR analysis of ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=5/condition). Data represent mean ± SEM, Mann-Whitney test ( Cdk2 ), and unpaired t-test ( Cdk4 and Cdk6 ). ( C ) Flow cytometry quantification of p-RB1 fluorescence intensity in ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=3/condition). Data represent mean ± SEM, unpaired t-test. *P < 0.05. ( D and E ) Representative IF staining of p-RB1 (green) and IB4 (red) in the peri-optic nerve and mid-plexus regions ( D ) and corresponding quantification of the peri-optic nerve region ( E ) of retinas from vehicle-treated PBS (n=6), palbociclib-treated PBS (n=5), vehicle-treated BMP9/10ib (n=6), and palbociclib-treated BMP9/10ib (n=6) mice. Data in ( E ) represent individual retinas and mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test. ns, not significant; *P < 0.05, **P < 0.01. Scale bars in ( D ), 50 μm. ( F ) Representative H&E and IHC staining of p-RB1 in 4 μm skin sections of HHT2 patients. Te, telangiectasia; N, normal vessel; Ba, basilar cells.

    Journal: bioRxiv

    Article Title: CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia

    doi: 10.1101/2023.09.15.554413

    Figure Lengend Snippet: ( A ) Heatmap analysis of a high-throughput cell cycle protein ELISA of ECs isolated from livers of P6 BMP9/10ib mice vs. PBS control littermates. Data represent n=2 independent analyses (n represents one litter of pups combined for each condition; PBS, n=6 mice; BMP9/10ib, n=7 mice). ( B ) qPCR analysis of ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=5/condition). Data represent mean ± SEM, Mann-Whitney test ( Cdk2 ), and unpaired t-test ( Cdk4 and Cdk6 ). ( C ) Flow cytometry quantification of p-RB1 fluorescence intensity in ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=3/condition). Data represent mean ± SEM, unpaired t-test. *P < 0.05. ( D and E ) Representative IF staining of p-RB1 (green) and IB4 (red) in the peri-optic nerve and mid-plexus regions ( D ) and corresponding quantification of the peri-optic nerve region ( E ) of retinas from vehicle-treated PBS (n=6), palbociclib-treated PBS (n=5), vehicle-treated BMP9/10ib (n=6), and palbociclib-treated BMP9/10ib (n=6) mice. Data in ( E ) represent individual retinas and mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test. ns, not significant; *P < 0.05, **P < 0.01. Scale bars in ( D ), 50 μm. ( F ) Representative H&E and IHC staining of p-RB1 in 4 μm skin sections of HHT2 patients. Te, telangiectasia; N, normal vessel; Ba, basilar cells.

    Article Snippet: Biopsies were processed, sectioned (4 μm), and stained with hematoxylin/eosin (H&E) and anti-p-RB1 (Ser807/811) antibody (Cell Signaling Technology, # 4277) at HistoWiz.

    Techniques: High Throughput Screening Assay, Enzyme-linked Immunosorbent Assay, Isolation, Control, MANN-WHITNEY, Flow Cytometry, Fluorescence, Staining, Immunohistochemistry