eft 3 p (Addgene inc)
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eft 3 p
Eft 3 P, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eft/pRS316-GFP-COF1+(Plasmid+%2331843)/pmc12227745-69-16-32
Average 93 stars, based on 2 article reviews
Eft 3 P, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+eft/pRS316-GFP-COF1+(Plasmid+%2331843)/pmc12227745-69-16-32
Average 93 stars, based on 2 article reviews
eft 3 p - by Bioz Stars,
2026-10
93/100 stars
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Related Articles
Plasmid Preparation:Article Title: AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor Article Snippet: The guide RNA sequence (5′-GTGGTAAAACGGACAATCAG-3′) targeting the first exon of aipr-1 was inserted into P u6::unc-119 sgRNA (Addgene plasmid 46169) to replace unc-119 sgRNA . .. The P u6::aipr-1 sgRNA plasmid thus produced ( Article Title: Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans Article Snippet: .. Prepare a 10 μL microinjection mixture with the final concentration of each component as follows: Article Title: Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo Article Snippet: .. The injection mix to generate the GFP for the creation of the swsn-1 :: egfp LoxP insertion contained: 100 ng/μl U6::gRNA target construct, 20 ng/μl pDD268 eGFP SEC vector with 150 bp swsn-1 Left Homology arm and swsn-1 600 bp Right Homology arm, 50 ng/μl Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: PCR-confirmed edited genomic loci were further validated using DNA sequencing (Macrogen Europe). loxP sites were integrated in the endogenous locus of pop-1 via co-conversion in a pha-1(e2123 ts ) background. .. Injection mixes contained a combination of 30-50 ng/μl Article Title: In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9 Article Snippet: .. pDD162 , Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: .. Injection mixes with a total volume of 50 μl were prepared in milliQ H 2 O and contained a combination of 30-50 ng/μl Produced:Article Title: AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor Article Snippet: The guide RNA sequence (5′-GTGGTAAAACGGACAATCAG-3′) targeting the first exon of aipr-1 was inserted into P u6::unc-119 sgRNA (Addgene plasmid 46169) to replace unc-119 sgRNA . .. The P u6::aipr-1 sgRNA plasmid thus produced ( Selection:Article Title: Tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division Article Snippet: For ebp-2 ::mKate2, 10 ng/μL pDD162 P eft-3 ::Cas9 with sgRNA targeting the C terminus of the ebp-2 locus (Addgene 47549) ( 73 ), 10 ng/μL repair template, and 65 ng/μL selection marker pRF4 were used. .. For lin-5 4A, 50 ng/μL Marker:Article Title: Tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division Article Snippet: For ebp-2 ::mKate2, 10 ng/μL pDD162 P eft-3 ::Cas9 with sgRNA targeting the C terminus of the ebp-2 locus (Addgene 47549) ( 73 ), 10 ng/μL repair template, and 65 ng/μL selection marker pRF4 were used. .. For lin-5 4A, 50 ng/μL Article Title: Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans Article Snippet: .. Prepare a 10 μL microinjection mixture with the final concentration of each component as follows: Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: PCR-confirmed edited genomic loci were further validated using DNA sequencing (Macrogen Europe). loxP sites were integrated in the endogenous locus of pop-1 via co-conversion in a pha-1(e2123 ts ) background. .. Injection mixes contained a combination of 30-50 ng/μl Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: .. Injection mixes with a total volume of 50 μl were prepared in milliQ H 2 O and contained a combination of 30-50 ng/μl Injection:Article Title: Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo Article Snippet: .. The injection mix contained the following: U6::gRNA target construct (100 ng/μl), pDD268 eGFP SEC vector (20 ng/μl) with 150-bp swsn-1 left homology arm and swsn-1 600-bp right homology arm, Article Title: Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo Article Snippet: .. The injection mix to generate the GFP for the creation of the swsn-1 :: egfp LoxP insertion contained: 100 ng/μl U6::gRNA target construct, 20 ng/μl pDD268 eGFP SEC vector with 150 bp swsn-1 Left Homology arm and swsn-1 600 bp Right Homology arm, 50 ng/μl Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: PCR-confirmed edited genomic loci were further validated using DNA sequencing (Macrogen Europe). loxP sites were integrated in the endogenous locus of pop-1 via co-conversion in a pha-1(e2123 ts ) background. .. Injection mixes contained a combination of 30-50 ng/μl Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: .. Injection mixes with a total volume of 50 μl were prepared in milliQ H 2 O and contained a combination of 30-50 ng/μl Construct:Article Title: Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo Article Snippet: .. The injection mix contained the following: U6::gRNA target construct (100 ng/μl), pDD268 eGFP SEC vector (20 ng/μl) with 150-bp swsn-1 left homology arm and swsn-1 600-bp right homology arm, Article Title: Dose-dependent functions of SWI/SNF BAF in permitting and inhibiting cell proliferation in vivo Article Snippet: .. The injection mix to generate the GFP for the creation of the swsn-1 :: egfp LoxP insertion contained: 100 ng/μl U6::gRNA target construct, 20 ng/μl pDD268 eGFP SEC vector with 150 bp swsn-1 Left Homology arm and swsn-1 600 bp Right Homology arm, 50 ng/μl Microinjection:Article Title: Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans Article Snippet: .. Prepare a 10 μL microinjection mixture with the final concentration of each component as follows: Concentration Assay:Article Title: Targeting endogenous proteins for spatial and temporal knockdown using auxin-inducible degron in Caenorhabditis elegans Article Snippet: .. Prepare a 10 μL microinjection mixture with the final concentration of each component as follows: Polyacrylamide Gel Electrophoresis:Article Title: C. elegans Runx/CBFβ suppresses POP-1 TCF to convert asymmetric to proliferative division of stem cell-like seam cells Article Snippet: PCR-confirmed edited genomic loci were further validated using DNA sequencing (Macrogen Europe). loxP sites were integrated in the endogenous locus of pop-1 via co-conversion in a pha-1(e2123 ts ) background. .. Injection mixes contained a combination of 30-50 ng/μl |
