p akt  (Bioss)

Journal: Journal of Inflammation Research

Article Title: Yin-Hua Li-Shi Formula Exerts Anti-Atopic Dermatitis Effects Through Luteolin Targeting AKT/MAPK-IL-6/IL-17 Axis

doi: 10.2147/JIR.S594212

Figure Lengend Snippet: ( A ) Venn diagram depicting the overlap between active compound targets and AD-associated targets. ( B ) “Drug-Component-Target” interaction network, representing active YHLS constituents and their potential AD-related targets. ( C–E ) GO functional enrichment plots presenting the top 10 significantly enriched terms in the BP, CC, and MF categories, respectively. ( F ) Bar graph illustrating the 16 most significantly enriched KEGG pathways. ( G ) KEGG-Target pathway network, where crimson dots indicate the core targets in the K-T network. ( H ) PPI network of YHLS targets in AD treatment, along with the derivation of the core subnetwork via two rounds of scoring and filtering. In this network, gene importance is reflected by color intensity: darker shades correspond to higher functional significance. ( I ) Visualization of the key targets (MAPK1, AKT1) and the five YHLS-derived chemical components that interact with these targets.

Article Snippet: Membranes were blocked with 5% skim milk in TBST for 30 minutes, then incubated overnight at 4°C with primary antibodies: AKT1 (1:2000, ET1609-47), p-AKT1 (1:1000, ET1612-73), p-MAPK1 (1:1000, ER1903-01), GAPDH (1:5000, ET1601-4; all HUABIO) and MAPK1 (1:5000, 51,068-1-AP, PTG).

Techniques: Functional Assay, Derivative Assay

Journal: Journal of Inflammation Research

Article Title: Yin-Hua Li-Shi Formula Exerts Anti-Atopic Dermatitis Effects Through Luteolin Targeting AKT/MAPK-IL-6/IL-17 Axis

doi: 10.2147/JIR.S594212

Figure Lengend Snippet: Schematic illustrations of molecular docking between core components of YHLS and key target proteins. Specifically, the docking complexes of diosgenin-AKT1, beta-carotene-AKT1, kaempferol-AKT1, luteolin-AKT1, quercetin-AKT1, luteolin-MAPK1, and quercetin-MAPK1 are corresponding to subpanels ( A–G ) in sequence.

Article Snippet: Membranes were blocked with 5% skim milk in TBST for 30 minutes, then incubated overnight at 4°C with primary antibodies: AKT1 (1:2000, ET1609-47), p-AKT1 (1:1000, ET1612-73), p-MAPK1 (1:1000, ER1903-01), GAPDH (1:5000, ET1601-4; all HUABIO) and MAPK1 (1:5000, 51,068-1-AP, PTG).

Techniques: Beta-Carotene, Sequencing

Journal: Journal of Inflammation Research

Article Title: Yin-Hua Li-Shi Formula Exerts Anti-Atopic Dermatitis Effects Through Luteolin Targeting AKT/MAPK-IL-6/IL-17 Axis

doi: 10.2147/JIR.S594212

Figure Lengend Snippet: ( A ) Impact of YHLS on serum IgE levels: high-dose YHLS reduced serum IgE in MC903-induced mice (n=3). ( B–D ) YHLS-mediated changes in mRNA expression of IL-6, IL-17, and TNF-α in ear tissues. ( E and F ) Representative Western blot bands of p-AKT1, AKT1, p-MAPK1, MAPK1. ( G and H ) Quantitative analysis of p-AKT1/AKT1 and p-MAPK1/MAPK1 phosphorylation ratios. Data are expressed as mean ± SEM (n=3), * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

Article Snippet: Membranes were blocked with 5% skim milk in TBST for 30 minutes, then incubated overnight at 4°C with primary antibodies: AKT1 (1:2000, ET1609-47), p-AKT1 (1:1000, ET1612-73), p-MAPK1 (1:1000, ER1903-01), GAPDH (1:5000, ET1601-4; all HUABIO) and MAPK1 (1:5000, 51,068-1-AP, PTG).

Techniques: Expressing, Western Blot, Phospho-proteomics