osteologic multitest slides (Biocoat)

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Biocoat osteologic multitest slides

Matairesinol did not affect survival or anti-resorptive activity of mature osteoclasts. (A) Mature osteoclasts were plated on BioCoat slides and treated for 24 h with the indicated concentrations of matairesinol. Cells were fixed, permeabilized, stained with TRAP, and photographed under a light microscope. TRAP-positive multinucleated cells were counted. (B) Attached cells on BioCoat Osteologic <t>MultiTest</t> slides were removed and photographed under a light microscope. Pit areas were quantified using the ImageJ program.

Osteologic Multitest Slides, supplied by Biocoat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/osteologic+multitest+slides/osteologic+multitest+slides/pmc03903021-69-11-10
Average 90 stars, based on 1 article reviews

osteologic multitest slides - by Bioz Stars, 2026-10

90/100 stars

Images

1) Product Images from "Anti-osteoclastogenic activity of matairesinol via suppression of p38/ERK-NFATc1 signaling axis"

Article Title: Anti-osteoclastogenic activity of matairesinol via suppression of p38/ERK-NFATc1 signaling axis

Journal: BMC Complementary and Alternative Medicine

doi: 10.1186/1472-6882-14-35

Figure Legend Snippet: Matairesinol did not affect survival or anti-resorptive activity of mature osteoclasts. (A) Mature osteoclasts were plated on BioCoat slides and treated for 24 h with the indicated concentrations of matairesinol. Cells were fixed, permeabilized, stained with TRAP, and photographed under a light microscope. TRAP-positive multinucleated cells were counted. (B) Attached cells on BioCoat Osteologic MultiTest slides were removed and photographed under a light microscope. Pit areas were quantified using the ImageJ program.

Techniques Used: Activity Assay, Staining, Light Microscopy

Cell Culture:

Article Title: Lysophosphatidic acid stimulates osteoclast fusion through OC-STAMP and P2X7 receptor signaling.
Article Snippet: Bone is continuously remodeled by bone formation and resorption, and cooperative bone metabolism is precisely regulated to maintain homeostasis.. Osteoclasts, which are responsible for bone resorption, are differentiated through multiple steps that include cell fusion at the last step of differentiation, yielding multinuclear cells.. However, the factors involved in and the precise mechanism of cell fusion are still unknown.

Activity Assay:

Article Title: Anti-osteoclastogenic activity of matairesinol via suppression of p38/ERK-NFATc1 signaling axis
Article Snippet: .. For measuring the resorptive activity of mature osteoclasts, osteoclasts and osteoblasts were co-cultured on BioCoat Osteologic MultiTest slides, and treated with matairesinol for 24 h. ..

Light Microscopy:

Article Title: Repositioning Potential of PAK4 to Osteoclastic Bone Resorption.
Article Snippet: .. BMMs that were differentiated into pre-osteoclasts on BioCoat Osteologic MultiTest slides in the presence of M-CSF (30 ng/ml) and RANKL (10 ng/ml) were treated with PF-3758309 for 24 h. TRAP-stained cells on the slides were photographed under a light microscope at 40× magnification. ..

Article Title: Anti-osteoclastogenic activity of matairesinol via suppression of p38/ERK-NFATc1 signaling axis
Article Snippet: .. Co-cultured cells were detached from the collagen-coated dishes, re-plated on BioCoat Osteologic MultiTest slides in a 96-well plate, and treated with matairesinol for 24 h. Cells on these slides were stained for TRAP and photographed under a light microscope at 40× magnification. ..

Article Title: Fisetin Inhibits Osteoclast Differentiation via Downregulation of p38 and c-Fos-NFATc1 Signaling Pathways
Article Snippet: .. Co-cultured cells were detached from the collagen-coated dishes, replated on BioCoat Osteologic MultiTest slides in 96-well plates, and treated with fisetin for 24 h. The cells on the BioCoat slides were stained for TRAP, and photographs were taken under a light microscope at 40x magnification. .. To observe resorption pits, cells on the BioCoat slides were washed with PBS and treated with 5% sodium hypochlorite for 5 min. After washing the plate with PBS buffer and drying, it was photographed under a light microscope.

Staining:

In Vitro:

Article Title: Osteoprotective Effects of Polysaccharide-Enriched Hizikia fusiforme Processing Byproduct In Vitro and In Vivo Models.
Article Snippet: The traditional manufacturing method used to produce goods from Hizikia fusiforme, utilizes extraction steps with hot water.. The byproduct (of hot water extraction) is rich in polysaccharide and is considered a waste.. To evaluate the osteogenic effects of the byproduct of H. fusiforme (HFB), osteogenic cells and animal models were used to test it effects on osteogenesis.

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