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osteogenic induction medium with shikonin  (MedChemExpress)


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    MedChemExpress osteogenic induction medium with shikonin
    Osteogenic Induction Medium With Shikonin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteogenic+induction+medium/Shikonin/pmc13000938-85-57-64
    Average 96 stars, based on 89 article reviews
    osteogenic induction medium with shikonin - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Control:

    Article Title: Implantation awakens peri-implant osteogenic potential via Snx5-EGFR axis-mediated mechanical transduction.
    Article Snippet: .. In the Baf-A1 treatment group, 1 nmol/L Baf-A1 (HY-100558, MCE) was incorporated into the osteogenic induction medium, while an equivalent volume of DMSO (D103274, Aladin) was administered to the control cells. .. The EGF treatment group received supplementation with 0.01 nmol/L EGF recombinant protein (HY-P7067, MCE) solubilized in non-enzymatic water within the osteogenic induction medium, and an equal volume of PBS buffer was added to the control cells.

    Article Title: Implantation awakens peri-implant osteogenic potential via Snx5-EGFR axis-mediated mechanical transduction
    Article Snippet: .. In the Baf-A1 treatment group, 1 nmol/L Baf-A1 (HY-100558, MCE) was incorporated into the osteogenic induction medium, while an equivalent volume of DMSO (D103274, Aladin) was administered to the control cells. .. The EGF treatment group received supplementation with 0.01 nmol/L EGF recombinant protein (HY-P7067, MCE) solubilized in non-enzymatic water within the osteogenic induction medium, and an equal volume of PBS buffer was added to the control cells.

    Article Title: Osteogenic effect of crocin in human periodontal ligament stem cells via Wnt/β-catenin signaling.
    Article Snippet: Periodontitis is an inflammatory disease and the most common cause of tooth loss in adults.. Reconstruction of the complex tooth support system (i.e., periodontal ligament, alveolar bone, and gingiva) after periodontitisinduced periodontal tissue damage faces enormous challenges (Michaud et al., 2017).. Although conventional therapies focused on periodontal inflammation control can halt or delay disease progression, periodontal regeneration remains an elusive goal (Kinane et al., 2017).

    Cell Culture:

    Article Title: RNA-sequencing-based elucidation of the mechanism underlying aFGF mediated regulation of BMSCs via the PI3K-AKT pathway and its implications for rotator cuff injury repair.
    Article Snippet: .. For osteogenic induction: BMSCs were seeded in 6-well plates at 5 × 104 cells/cm2 and cultured in osteogenic induction medium supplemented with dexamethasone (MCE, Monmouth Junction, NJ, Fig. 1 Flow chart of this study USA), β-glycerophosphate (Sigma-Aldrich, St. Louis, MO, USA), and ascorbic acid (MCE) for 21 days. ..

    Article Title: Functional mechanism and clinical implications of miR-455-5p in delayed fracture healing
    Article Snippet: The cells were cultured in α-MEM (12571063,Thermo Fishier Scientific) supplemented with 10% fetal bovine serum (A5670801, Thermo Fisher Scientific) and 1 × 10 5 U/L penicillin-streptomycin under standard conditions of 37 °C and 5% CO 2 in a humidified incubator. .. When the cells reached approximately 80% confluence, the inoculation density was set at 2 × 10 4 cells/cm 2 , and the culture medium was replaced with osteogenic induction medium containing 5 mmol/L L-glycerophosphate (HY-P2733, MedChemExpress), 100 nmol/L dexamethasone (HY-14648, MedChemExpress), and 50 μg/L ascorbic acid (HY-B0166, MedChemExpress).Cells were then cultured for 0, 7, and 14 days to simulate different stages of the bone regeneration process. ..

    Article Title: RNA-sequencing-based elucidation of the mechanism underlying aFGF mediated regulation of BMSCs via the PI3K-AKT pathway and its implications for rotator cuff injury repair
    Article Snippet: .. For osteogenic induction: BMSCs were seeded in 6-well plates at 5 × 10 4 cells/cm 2 and cultured in osteogenic induction medium supplemented with dexamethasone (MCE, Monmouth Junction, NJ, USA), β-glycerophosphate (Sigma-Aldrich, St. Louis, MO, USA), and ascorbic acid (MCE) for 21 days. ..

    Article Title: Functional mechanism and clinical implications of miR-455-5p in delayed fracture healing.
    Article Snippet: The cells were cultured in α-MEM (12571063,Thermo Fishier Scientific) supplemented with 10% fetal bovine serum (A5670801, Thermo Fisher Scientific) and 1 × 105 U/L penicillin-streptomycin under standard conditions of 37 °C and 5% CO2 in a humidified incubator. .. When the cells reached approximately 80% confluence, the inoculation density was set at 2 × 104 cells/cm2, and the culture medium was replaced with osteogenic induction medium containing 5 mmol/L L-glycerophosphate (HY-P2733, MedChemExpress), 100 nmol/L dexamethasone (HY-14648, MedChemExpress), and 50 μg/L ascorbic acid (HY-B0166, MedChemExpress).Cells were then cultured for 0, 7, and 14 days to simulate different stages of the bone regeneration process. ..

    Article Title: Osteogenic effect of crocin in human periodontal ligament stem cells via Wnt/β-catenin signaling.
    Article Snippet: Periodontitis is an inflammatory disease and the most common cause of tooth loss in adults.. Reconstruction of the complex tooth support system (i.e., periodontal ligament, alveolar bone, and gingiva) after periodontitisinduced periodontal tissue damage faces enormous challenges (Michaud et al., 2017).. Although conventional therapies focused on periodontal inflammation control can halt or delay disease progression, periodontal regeneration remains an elusive goal (Kinane et al., 2017).



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    Co-culture with SSCs rescues the function of irradiated <t>osteogenic</t> precursor cells. (A, B) Cell apoptosis was analyzed by flow cytometry with Annexin V-PE/7AAD double staining. (A) Representative flow cytometry plots. (B) Quantitative analysis of the apoptotic rate. (C, D) ALP activity was assessed. (C) Representative ALP staining images (Scale bar: 50 μm). (D) Quantitative analysis of the relative ALP activity. (E, F) Mineralization capacity was evaluated using Alizarin Red S staining. (E) Representative staining images of mineralized nodules (Scale bar: 100 μm). (F) Quantitative analysis of the relative mineralization level. (G, H) Cell migration was determined by a migration assay. (G) Representative images of migrated cells (Scale bar: 50 μm). (H) Quantitative analysis of the relative cell migration level. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01).
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    MedChemExpress osteogenic induction medium with shikonin
    Co-culture with SSCs rescues the function of irradiated <t>osteogenic</t> precursor cells. (A, B) Cell apoptosis was analyzed by flow cytometry with Annexin V-PE/7AAD double staining. (A) Representative flow cytometry plots. (B) Quantitative analysis of the apoptotic rate. (C, D) ALP activity was assessed. (C) Representative ALP staining images (Scale bar: 50 μm). (D) Quantitative analysis of the relative ALP activity. (E, F) Mineralization capacity was evaluated using Alizarin Red S staining. (E) Representative staining images of mineralized nodules (Scale bar: 100 μm). (F) Quantitative analysis of the relative mineralization level. (G, H) Cell migration was determined by a migration assay. (G) Representative images of migrated cells (Scale bar: 50 μm). (H) Quantitative analysis of the relative cell migration level. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01).
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    Image Search Results


    In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Journal: Bioactive Materials

    Article Title: A motion-activated skeletal “Pacemaker” for systemic bone remodeling via oral piezoelectric microspheres

    doi: 10.1016/j.bioactmat.2026.05.051

    Figure Lengend Snippet: In vitro evaluation of the osteogenic-promoting capability of BTO@ALN. (A–D) Immunofluorescence staining of osteoblasts differentiated from primary mouse bone marrow–derived mesenchymal stem cells (BMSCs) (red: actin; green: RUNX2 and ATF4) and corresponding quantitative analysis of fluorescence intensity. (E–H) Alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining of osteoblasts derived from primary mouse BMSCs and quantitative analysis of staining intensity.

    Article Snippet: Osteogenic differentiation was induced by culturing BMSCs in osteogenic induction medium (Procell, Wuhan, China).

    Techniques: In Vitro, Immunofluorescence, Staining, Derivative Assay, Fluorescence

    Co-culture with SSCs rescues the function of irradiated osteogenic precursor cells. (A, B) Cell apoptosis was analyzed by flow cytometry with Annexin V-PE/7AAD double staining. (A) Representative flow cytometry plots. (B) Quantitative analysis of the apoptotic rate. (C, D) ALP activity was assessed. (C) Representative ALP staining images (Scale bar: 50 μm). (D) Quantitative analysis of the relative ALP activity. (E, F) Mineralization capacity was evaluated using Alizarin Red S staining. (E) Representative staining images of mineralized nodules (Scale bar: 100 μm). (F) Quantitative analysis of the relative mineralization level. (G, H) Cell migration was determined by a migration assay. (G) Representative images of migrated cells (Scale bar: 50 μm). (H) Quantitative analysis of the relative cell migration level. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01).

    Journal: Dose-Response

    Article Title: Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling Pathway

    doi: 10.1177/15593258261440983

    Figure Lengend Snippet: Co-culture with SSCs rescues the function of irradiated osteogenic precursor cells. (A, B) Cell apoptosis was analyzed by flow cytometry with Annexin V-PE/7AAD double staining. (A) Representative flow cytometry plots. (B) Quantitative analysis of the apoptotic rate. (C, D) ALP activity was assessed. (C) Representative ALP staining images (Scale bar: 50 μm). (D) Quantitative analysis of the relative ALP activity. (E, F) Mineralization capacity was evaluated using Alizarin Red S staining. (E) Representative staining images of mineralized nodules (Scale bar: 100 μm). (F) Quantitative analysis of the relative mineralization level. (G, H) Cell migration was determined by a migration assay. (G) Representative images of migrated cells (Scale bar: 50 μm). (H) Quantitative analysis of the relative cell migration level. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01).

    Article Snippet: After irradiation and corresponding interventions, cells were cultured in osteogenic induction medium (iXCells Biotechnologies, San Diego, CA, Cat. No. MD-0006) for 7 days.

    Techniques: Co-Culture Assay, Irradiation, Flow Cytometry, Double Staining, Activity Assay, Staining, Migration, Two Tailed Test

    SSCs exert rescue effects via the Wnt/β-catenin signaling pathway. (A) Representative ALP staining images of cells in each group (Scale bar: 50 μm). (B) Quantitative analysis of ALP activity in each group. (C) Representative Alizarin Red S staining images of cells in each group (Scale bar: 100 μm). (D) Quantitative analysis of Alizarin Red S staining in each group. (E) Relative mRNA expression levels of osteogenic marker genes ( Runx2 , Col1a1 , and OCN ) detected by qRT-PCR. GAPDH was used as an internal reference gene. (F) Representative Western blot images showing the expression levels of RUNX2, COL1A1, OCN, and β-catenin in each group. GAPDH was used as a loading control. (G) Quantitative analysis of Western blot results (gray value ratio of target protein to GAPDH) in each group. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).

    Journal: Dose-Response

    Article Title: Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling Pathway

    doi: 10.1177/15593258261440983

    Figure Lengend Snippet: SSCs exert rescue effects via the Wnt/β-catenin signaling pathway. (A) Representative ALP staining images of cells in each group (Scale bar: 50 μm). (B) Quantitative analysis of ALP activity in each group. (C) Representative Alizarin Red S staining images of cells in each group (Scale bar: 100 μm). (D) Quantitative analysis of Alizarin Red S staining in each group. (E) Relative mRNA expression levels of osteogenic marker genes ( Runx2 , Col1a1 , and OCN ) detected by qRT-PCR. GAPDH was used as an internal reference gene. (F) Representative Western blot images showing the expression levels of RUNX2, COL1A1, OCN, and β-catenin in each group. GAPDH was used as a loading control. (G) Quantitative analysis of Western blot results (gray value ratio of target protein to GAPDH) in each group. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001).

    Article Snippet: After irradiation and corresponding interventions, cells were cultured in osteogenic induction medium (iXCells Biotechnologies, San Diego, CA, Cat. No. MD-0006) for 7 days.

    Techniques: Staining, Activity Assay, Expressing, Marker, Quantitative RT-PCR, Western Blot, Control, Two Tailed Test

    SSCs alleviate the radiation-induced bone injury in mice. (A–G) Micro-CT analysis of bone microstructure. (A) Representative micro-CT images of femurs. Quantitative analysis of (B) bone mineral density (BMD), (C) bone volume fraction (BV/TV), (D) trabecular thickness (Tb.Th), (E) trabecular number (Tb.N), (F) connectivity density (Conn.D), and (G) trabecular separation (Tb.Sp) at 2- and 4-weeks post irradiation. (H–K) Histological analysis (Scale bar: 100 μm). (H) H&E staining showing steatosis (arrows) and (I) quantitative analysis of steatotic lesions per field. (J) TRAP staining showing osteoclasts (arrows) and (K) quantitative analysis of osteoclast number per field. (L–O) Immunohistochemical staining of osteogenic markers (Scale bar: 100 μm). (L) Osterix staining and (M) quantitative analysis of Osterix-positive area. (N) β-catenin staining and (O) quantitative analysis of β-catenin-positive area. All experiments were conducted in three groups: Control, irradiation (IR), and IR plus SSC (IR+SSC) at 2- and 4-weeks post-irradiation. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Journal: Dose-Response

    Article Title: Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling Pathway

    doi: 10.1177/15593258261440983

    Figure Lengend Snippet: SSCs alleviate the radiation-induced bone injury in mice. (A–G) Micro-CT analysis of bone microstructure. (A) Representative micro-CT images of femurs. Quantitative analysis of (B) bone mineral density (BMD), (C) bone volume fraction (BV/TV), (D) trabecular thickness (Tb.Th), (E) trabecular number (Tb.N), (F) connectivity density (Conn.D), and (G) trabecular separation (Tb.Sp) at 2- and 4-weeks post irradiation. (H–K) Histological analysis (Scale bar: 100 μm). (H) H&E staining showing steatosis (arrows) and (I) quantitative analysis of steatotic lesions per field. (J) TRAP staining showing osteoclasts (arrows) and (K) quantitative analysis of osteoclast number per field. (L–O) Immunohistochemical staining of osteogenic markers (Scale bar: 100 μm). (L) Osterix staining and (M) quantitative analysis of Osterix-positive area. (N) β-catenin staining and (O) quantitative analysis of β-catenin-positive area. All experiments were conducted in three groups: Control, irradiation (IR), and IR plus SSC (IR+SSC) at 2- and 4-weeks post-irradiation. All data are presented as mean ± SD, with statistical significance determined by unpaired two-tailed Student’s t-test (* p < 0.05; ** p < 0.01; *** p < 0.001)

    Article Snippet: After irradiation and corresponding interventions, cells were cultured in osteogenic induction medium (iXCells Biotechnologies, San Diego, CA, Cat. No. MD-0006) for 7 days.

    Techniques: Micro-CT, Irradiation, Staining, Immunohistochemical staining, Control, Two Tailed Test