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optiprep step gradient  (Axis-Shield Diagnostics)

 
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    Axis-Shield Diagnostics optiprep step gradient
    Optiprep Step Gradient, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optiprep+step+gradient/optiprep+gradient/pm38904846-46-11-14
    Average 90 stars, based on 1 article reviews
    optiprep step gradient - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Calycosin promotes axon growth by inhibiting PTPRS and alleviates spinal cord injury.
    Article Snippet: Our former studies have identified the alleviating effect of Calycosin (CA) on spinal cord injury (SCI).. In this study, our purpose is to explore the influence of CA on SCI from the perspective of promoting axon growth.. The SCI animal model was constructed by spinal cord compression, wherein rat primary cortex neuronal isolation was performed, and the axonal growth restriction cell model was established via chondroitin sulfate proteoglycan (CSPG) treatment.

    Positive Control:

    Article Title: Olfactory Ensheathing Cell Transplantation after a Complete Spinal Cord Transection Mediates Neuroprotective and Immunomodulatory Mechanisms to Facilitate Regeneration
    Article Snippet: Cortices were chopped and digested with warm papain (2 mg/ml; Worthington) in Hibernate-A medium with P/S and l -glu. .. Neurons were enriched with an OptiPrep step gradient (Axis-Shield), resuspended in Neurobasal medium (Invitrogen), and counted before they were added to the positive control, pre-coated laminin + PLL (Laminin 10 μg/ml; Invitrogen), the neutral control, PLL only (0.05 mg/ml), or either wild-type or transgenic OECs + PLL wells at a concentration of 100,000 cells per well. .. After plating the cortical neurons, cultures were maintained for an additional 24 h. Cultures were fixed in 4% paraformaldehyde for 15 min, washed with buffer, and neurons were visualized with anti-β-3-tubulin (1:1500; Covance).

    Control:

    Article Title: Olfactory Ensheathing Cell Transplantation after a Complete Spinal Cord Transection Mediates Neuroprotective and Immunomodulatory Mechanisms to Facilitate Regeneration
    Article Snippet: Cortices were chopped and digested with warm papain (2 mg/ml; Worthington) in Hibernate-A medium with P/S and l -glu. .. Neurons were enriched with an OptiPrep step gradient (Axis-Shield), resuspended in Neurobasal medium (Invitrogen), and counted before they were added to the positive control, pre-coated laminin + PLL (Laminin 10 μg/ml; Invitrogen), the neutral control, PLL only (0.05 mg/ml), or either wild-type or transgenic OECs + PLL wells at a concentration of 100,000 cells per well. .. After plating the cortical neurons, cultures were maintained for an additional 24 h. Cultures were fixed in 4% paraformaldehyde for 15 min, washed with buffer, and neurons were visualized with anti-β-3-tubulin (1:1500; Covance).

    Transgenic Assay:

    Article Title: Olfactory Ensheathing Cell Transplantation after a Complete Spinal Cord Transection Mediates Neuroprotective and Immunomodulatory Mechanisms to Facilitate Regeneration
    Article Snippet: Cortices were chopped and digested with warm papain (2 mg/ml; Worthington) in Hibernate-A medium with P/S and l -glu. .. Neurons were enriched with an OptiPrep step gradient (Axis-Shield), resuspended in Neurobasal medium (Invitrogen), and counted before they were added to the positive control, pre-coated laminin + PLL (Laminin 10 μg/ml; Invitrogen), the neutral control, PLL only (0.05 mg/ml), or either wild-type or transgenic OECs + PLL wells at a concentration of 100,000 cells per well. .. After plating the cortical neurons, cultures were maintained for an additional 24 h. Cultures were fixed in 4% paraformaldehyde for 15 min, washed with buffer, and neurons were visualized with anti-β-3-tubulin (1:1500; Covance).

    Concentration Assay:

    Article Title: Olfactory Ensheathing Cell Transplantation after a Complete Spinal Cord Transection Mediates Neuroprotective and Immunomodulatory Mechanisms to Facilitate Regeneration
    Article Snippet: Cortices were chopped and digested with warm papain (2 mg/ml; Worthington) in Hibernate-A medium with P/S and l -glu. .. Neurons were enriched with an OptiPrep step gradient (Axis-Shield), resuspended in Neurobasal medium (Invitrogen), and counted before they were added to the positive control, pre-coated laminin + PLL (Laminin 10 μg/ml; Invitrogen), the neutral control, PLL only (0.05 mg/ml), or either wild-type or transgenic OECs + PLL wells at a concentration of 100,000 cells per well. .. After plating the cortical neurons, cultures were maintained for an additional 24 h. Cultures were fixed in 4% paraformaldehyde for 15 min, washed with buffer, and neurons were visualized with anti-β-3-tubulin (1:1500; Covance).



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    Image Search Results


    Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Journal: BMC Neuroscience

    Article Title: Down-regulation of microglial activity attenuates axotomized nigral dopaminergic neuronal cell loss

    doi: 10.1186/1471-2202-14-112

    Figure Lengend Snippet: Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Article Snippet: The collected supernatant was loaded upon the top of the prepared OptiPrep step gradient (Sigma-Aldrich Co., St. Louis, USA) solution.

    Techniques: Isolation, Fractionation, Labeling, Cell Culture