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omp25 mcherry  (Addgene inc)


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    Structured Review

    Addgene inc omp25 mcherry
    HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization <t>with</t> <t>Omp25-mCherry</t> ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.
    Omp25 Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omp25+mcherry/pmCherry+C1+Omp25+(Plasmid+%23157758)/pmc12810550-287-13-14
    Average 94 stars, based on 3 article reviews
    omp25 mcherry - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism"

    Article Title: Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2025.111009

    HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization with Omp25-mCherry ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.
    Figure Legend Snippet: HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization with Omp25-mCherry ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.

    Techniques Used: Knock-Out

    Related Articles

    Transfection:

    Article Title: VDAC isoforms play distinct roles in modulating metabolism and maintaining mitochondrial function
    Article Snippet: .. Cells were transfected with HK1-GFP (Addgene, 21917) or HK2-GFP (Addgene, 21920) along with Omp25-mCherry (Addgene, 157758) using Lipofectamine TM Stem transfection reagent (ThermoFisher Scientific, STEM00008) according to the manufacturer’s protocol. .. The cells were washed and imaged in Fluorobrite DMEM (Gibco, A18967) with a 20x/0.75 N.A. objective in Leica TCS SP8 microscope.

    Article Title: Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism.
    Article Snippet: .. Cells were transfected with HK1-GFP (Addgene, Jo urn al Pr e-p roo f 17 21917) or HK2-GFP (Addgene, 21920) along with Omp25-mCherry (Addgene, 157758) using LipofectamineTM Stem transfection reagent (ThermoFisher Scientific, STEM00008) according to the manufacturer's protocol. .. The cells were washed and imaged in Fluorobrite DMEM (Gibco, A18967) with a 20x/0.75 N.A. objective in Leica TCS SP8 microscope.

    Article Title: Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism
    Article Snippet: .. Cells were transfected with HK1-GFP (Addgene, 21917) or HK2-GFP (Addgene, 21920) along with Omp25-mCherry (Addgene, 157758) using Lipofectamine Stem transfection reagent (Thermo Fisher Scientific, STEM00008) according to the manufacturer's protocol. .. The cells were washed and imaged in Fluorobrite DMEM (Gibco, A18967) with a 20x/0.75 N. A. objective in Leica TCS SP8 microscope.



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    HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization <t>with</t> <t>Omp25-mCherry</t> ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.
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    HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization <t>with</t> <t>Omp25-mCherry</t> ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.
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    Image Search Results


    HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization with Omp25-mCherry ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.

    Journal: The Journal of Biological Chemistry

    Article Title: Three mammalian VDAC isoforms distinctly regulate mitochondrial function and proteome to maintain cell metabolism

    doi: 10.1016/j.jbc.2025.111009

    Figure Lengend Snippet: HK1 mitochondrial localization is dependent on VDAC1 isoform . A , representative images of HK1-GFP ( green ) colocalization with Omp25-mCherry ( red ) shown in the merged image ( yellow ) for HeLa cells with WT, VDAC1 KO, VDAC2 KO, and VDAC3 KO. (Scale bar represents 50 μm). B , colocalization analysis of HK1 with Omp25 measured by Pearson’s correlation coefficient (PCC) shows a significant decrease in HK1 mitochondrial localization in VDAC1 KO. Data from three independent experiments are represented. The symbols represent PCC for each cell, and error bars indicate the SD from the mean. Significance was tested using one-way ANOVA followed by Dunnett's post hoc test (∗∗∗∗ p < 0.0001). KO, knockout; VDAC, voltage-dependent anion channel.

    Article Snippet: Cells were transfected with HK1-GFP (Addgene, 21917) or HK2-GFP (Addgene, 21920) along with Omp25-mCherry (Addgene, 157758) using Lipofectamine Stem transfection reagent (Thermo Fisher Scientific, STEM00008) according to the manufacturer's protocol.

    Techniques: Knock-Out