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nuclear dye hoechst 33342  (Beyotime)


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    Beyotime nuclear dye hoechst 33342
    Nuclear Dye Hoechst 33342, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 7421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+dyes/Hoechst+33342/pm41999902-253-73-81
    Average 99 stars, based on 7421 article reviews
    nuclear dye hoechst 33342 - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Double Staining:

    Article Title: A dissolvable microneedle patch co-delivering Zerumbone and ICG for combined chemo-photothermal therapy of melanoma.
    Article Snippet: Kits for apoptosis analysis (Annexin V-FITC/PI) were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). .. Calcein-AM/PI double-staining reagents, nuclear dyes (Hoechst 33342, DAPI), and organelle trackers (LysoTracker Red, MitoTracker Red CMXRos) were provided by Beyotime Biotechnology (Shanghai, China). .. Cell Culture Mouse-derived melanoma B16F10 and human melanoma A375 cell lines were provided by Wuhan Servicebio Technology Co., Ltd. (Wuhan, China).

    Article Title: A dissolvable microneedle patch co-delivering Zerumbone and ICG for combined chemo-photothermal therapy of melanoma
    Article Snippet: Kits for apoptosis analysis (Annexin V-FITC/PI) were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China). .. Calcein-AM/PI double-staining reagents, nuclear dyes (Hoechst 33342, DAPI), and organelle trackers (LysoTracker Red, MitoTracker Red CMXRos) were provided by Beyotime Biotechnology (Shanghai, China). .. Mouse-derived melanoma B16F10 and human melanoma A375 cell lines were provided by Wuhan Servicebio Technology Co., Ltd. (Wuhan, China).



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    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, <t>4′,6-diamidino-2-phenylindole.</t>
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    Image Search Results


    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    doi: 10.1126/sciadv.aed1296

    Figure Lengend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight followed by incubation with fluorophore-conjugated secondary antibodies (table S11) and nuclear dye 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific) at room temperature for 2 hours.

    Techniques: Staining, Microarray