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LTDR changed expression of the genes involved in lipid metabolism in the liver. To assess the effects of LTDR on gene expression, pieces of the livers from the adult, aged and LTDR groups were collected, total RNA was extracted, and qPCR was performed. Target gene expression (%) was determined by the comparative 2 −ΔΔCT quantification method with Gapdh as the reference gene. Time- and LTDR-induced changes in expression profile of Hmgcr , Srebf1 , Soat1 , Lxra , ApoB , Ldlr , ApoA , Scarb1 , <t>Npc1</t> , Lrp , Abca1 are presented at panels A–K . The bars shown are mean ± SEM values; for all the analysis n ≥ 5; * indicates significant differences compared to adults; # indicates significant differences between aged animals and the LTDR group; p < 0.05.
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LTDR changed expression of the genes involved in lipid metabolism in the liver. To assess the effects of LTDR on gene expression, pieces of the livers from the adult, aged and LTDR groups were collected, total RNA was extracted, and qPCR was performed. Target gene expression (%) was determined by the comparative 2 −ΔΔCT quantification method with Gapdh as the reference gene. Time- and LTDR-induced changes in expression profile of Hmgcr , Srebf1 , Soat1 , Lxra , ApoB , Ldlr , ApoA , Scarb1 , <t>Npc1</t> , Lrp , Abca1 are presented at panels A–K . The bars shown are mean ± SEM values; for all the analysis n ≥ 5; * indicates significant differences compared to adults; # indicates significant differences between aged animals and the LTDR group; p < 0.05.
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LTDR changed expression of the genes involved in lipid metabolism in the liver. To assess the effects of LTDR on gene expression, pieces of the livers from the adult, aged and LTDR groups were collected, total RNA was extracted, and qPCR was performed. Target gene expression (%) was determined by the comparative 2 −ΔΔCT quantification method with Gapdh as the reference gene. Time- and LTDR-induced changes in expression profile of Hmgcr , Srebf1 , Soat1 , Lxra , ApoB , Ldlr , ApoA , Scarb1 , <t>Npc1</t> , Lrp , Abca1 are presented at panels A–K . The bars shown are mean ± SEM values; for all the analysis n ≥ 5; * indicates significant differences compared to adults; # indicates significant differences between aged animals and the LTDR group; p < 0.05.
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LTDR changed expression of the genes involved in lipid metabolism in the liver. To assess the effects of LTDR on gene expression, pieces of the livers from the adult, aged and LTDR groups were collected, total RNA was extracted, and qPCR was performed. Target gene expression (%) was determined by the comparative 2 −ΔΔCT quantification method with Gapdh as the reference gene. Time- and LTDR-induced changes in expression profile of Hmgcr , Srebf1 , Soat1 , Lxra , ApoB , Ldlr , ApoA , Scarb1 , <t>Npc1</t> , Lrp , Abca1 are presented at panels A–K . The bars shown are mean ± SEM values; for all the analysis n ≥ 5; * indicates significant differences compared to adults; # indicates significant differences between aged animals and the LTDR group; p < 0.05.
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LTDR changed expression of the genes involved in lipid metabolism in the liver. To assess the effects of LTDR on gene expression, pieces of the livers from the adult, aged and LTDR groups were collected, total RNA was extracted, and qPCR was performed. Target gene expression (%) was determined by the comparative 2 −ΔΔCT quantification method with Gapdh as the reference gene. Time- and LTDR-induced changes in expression profile of Hmgcr , Srebf1 , Soat1 , Lxra , ApoB , Ldlr , ApoA , Scarb1 , Npc1 , Lrp , Abca1 are presented at panels A–K . The bars shown are mean ± SEM values; for all the analysis n ≥ 5; * indicates significant differences compared to adults; # indicates significant differences between aged animals and the LTDR group; p < 0.05.

Journal: Frontiers in Nutrition

Article Title: Long-term dietary restriction changes lipid homeostasis and consequently impairs testosterone production in aged Wistar rats

doi: 10.3389/fnut.2025.1665682

Figure Lengend Snippet: LTDR changed expression of the genes involved in lipid metabolism in the liver. To assess the effects of LTDR on gene expression, pieces of the livers from the adult, aged and LTDR groups were collected, total RNA was extracted, and qPCR was performed. Target gene expression (%) was determined by the comparative 2 −ΔΔCT quantification method with Gapdh as the reference gene. Time- and LTDR-induced changes in expression profile of Hmgcr , Srebf1 , Soat1 , Lxra , ApoB , Ldlr , ApoA , Scarb1 , Npc1 , Lrp , Abca1 are presented at panels A–K . The bars shown are mean ± SEM values; for all the analysis n ≥ 5; * indicates significant differences compared to adults; # indicates significant differences between aged animals and the LTDR group; p < 0.05.

Article Snippet: Used Applied Biosystems predesigned TaqMan Gene Expression Assays: Abca1 (Rn00710172_m1), Apob (01499054_m1), Cyp46a1 (Rn01430187_g1), Gapdh (Rn01462662_g1), Hmgc (Rn 00565598_m1), Lrp1 (Rn01503901), Ldlr (Rn00598442_m1), Npc1 (Rn01531821_m1), Soat1 (Rn_00579605_m1), Srebf1 (Rn01495769_m1); sequences of used forward and reverse primers are listed in .

Techniques: Expressing, Gene Expression, Targeted Gene Expression