npc1 Search Results


npc1  (R&D Systems)
92
R&D Systems npc1
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Npc1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/npc1/product/R&D Systems
Average 92 stars, based on 1 article reviews
npc1 - by Bioz Stars, 2026-05
92/100 stars
  Buy from Supplier
port a patch automated patch clamp system  (Nanion Technologies GmbH)
94
Nanion Technologies GmbH port a patch automated patch clamp system
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Port A Patch Automated Patch Clamp System, supplied by Nanion Technologies GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/port a patch automated patch clamp system/product/Nanion Technologies GmbH
Average 94 stars, based on 1 article reviews
port a patch automated patch clamp system - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
npc1 antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology npc1 antibody
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Npc1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/npc1 antibody/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
npc1 antibody - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
rabbit polyclonal anti npc1 antibody  (Rockland Immunochemicals)
90
Rockland Immunochemicals rabbit polyclonal anti npc1 antibody
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Rabbit Polyclonal Anti Npc1 Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti npc1 antibody/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
rabbit polyclonal anti npc1 antibody - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
anti npc1  (Atlas Antibodies)
85
Atlas Antibodies anti npc1
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Anti Npc1, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti npc1/product/Atlas Antibodies
Average 85 stars, based on 1 article reviews
anti npc1 - by Bioz Stars, 2026-05
85/100 stars
  Buy from Supplier
expressing npc1 gfp  (Addgene inc)
93
Addgene inc expressing npc1 gfp
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Expressing Npc1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expressing npc1 gfp/product/Addgene inc
Average 93 stars, based on 1 article reviews
expressing npc1 gfp - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
rabbit anti npc1  (Proteintech)
93
Proteintech rabbit anti npc1
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Rabbit Anti Npc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti npc1/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti npc1 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
single use planar patch clamp chips  (Nanion Technologies GmbH)
94
Nanion Technologies GmbH single use planar patch clamp chips
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Single Use Planar Patch Clamp Chips, supplied by Nanion Technologies GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/single use planar patch clamp chips/product/Nanion Technologies GmbH
Average 94 stars, based on 1 article reviews
single use planar patch clamp chips - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
mouse npc1 his6 eyfp expression plasmid  (Addgene inc)
86
Addgene inc mouse npc1 his6 eyfp expression plasmid
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Mouse Npc1 His6 Eyfp Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse npc1 his6 eyfp expression plasmid/product/Addgene inc
Average 86 stars, based on 1 article reviews
mouse npc1 his6 eyfp expression plasmid - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
conditional npc1 knockout mice  (Cyagen Biosciences)
93
Cyagen Biosciences conditional npc1 knockout mice
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Conditional Npc1 Knockout Mice, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conditional npc1 knockout mice/product/Cyagen Biosciences
Average 93 stars, based on 1 article reviews
conditional npc1 knockout mice - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
gene exp npc1 hs00264835 m1  (Thermo Fisher)
92
Thermo Fisher gene exp npc1 hs00264835 m1
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
Gene Exp Npc1 Hs00264835 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp npc1 hs00264835 m1/product/Thermo Fisher
Average 92 stars, based on 1 article reviews
gene exp npc1 hs00264835 m1 - by Bioz Stars, 2026-05
92/100 stars
  Buy from Supplier
a37573 recombinant npc1 protein  (Creative BioMart)
88
Creative BioMart a37573 recombinant npc1 protein
Fig. 6. Hindered phenol compound increase <t>NPC1</t> levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.
A37573 Recombinant Npc1 Protein, supplied by Creative BioMart, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a37573 recombinant npc1 protein/product/Creative BioMart
Average 88 stars, based on 1 article reviews
a37573 recombinant npc1 protein - by Bioz Stars, 2026-05
88/100 stars
  Buy from Supplier

Image Search Results


Fig. 6. Hindered phenol compound increase NPC1 levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: Free radical biology & medicine

Article Title: Discovery of sterically-hindered phenol compounds with potent cytoprotective activities against ox-LDL-induced retinal pigment epithelial cell death as a potential pharmacotherapy.

doi: 10.1016/j.freeradbiomed.2021.11.026

Figure Lengend Snippet: Fig. 6. Hindered phenol compound increase NPC1 levels and reduce intracellular ox-LDL caused cholesterol accumulation. (A) Serum starved ARPE-19 cells were treated with indicated doses of PMC for 18 h, cell lysates were collected and NPC1 protein levels were examined by western blot and imaged Li-COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine fold change. (B) ARPE-19 cells were treated with ox-LDL in the presence or absence of PMC, cell lysates were collected 18 h post treatment and western blot was performed to determine the relative protein levels of NPC-1 using Li- COR® infrared imaging system. The fluorescent intensity of NPC1 levels were normalized to the fluorescent intensity of α-tubulin to determine the relative expression levels of NPC1. (C) ARPE-19 cells were treated with ox-LDL for 8hr, cells were washed and then incubated with or without PMC. Cell lysates were collected and the total cellular cholesterol content was determined by normalizing to total cellular protein content. Serum starved untreated cells served as control. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Primary antibodies: PPARγ and vinculin (catalog # 2443S and 13901S, Cell Signaling, Danvers, MA), NPC1 (Catalog # MAB10105, R&D systems, Inc. Minneapolis, MN), alphatubulin (catalog # CP06, Calbiochem, MilliporeSigma, Burlington, G. Gnanaguru et al. Free Radical Biology and Medicine 178 (2022) 360–368 MA).

Techniques: Western Blot, Imaging, Expressing, Incubation, Control