Review



noti digested pcmv vector  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    TaKaRa noti digested pcmv vector
    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the <t>pCMV</t> control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Noti Digested Pcmv Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/noti+digested+pcmv+vector/pCMV-LacZ+Vector/pmc12769681-100-21-24
    Average 93 stars, based on 138 article reviews
    noti digested pcmv vector - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes"

    Article Title: Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes

    Journal: Human Genetics

    doi: 10.1007/s00439-025-02800-7

    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Figure Legend Snippet: Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.

    Techniques Used: Activity Assay, Luciferase, Plasmid Preparation, Cotransfection, Control

    Related Articles

    Plasmid Preparation:

    Article Title: Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes
    Article Snippet: .. The SLC6A14 insert was ligated to the EcoRI/BamHI-digested pTetOne vector (Clontech, 634310), and IRF1 , IRF2 inserts were ligated to the NotI-digested pCMV vector (Clontech, 631719), replacing LacZ . .. To generate the luciferase reporter plasmids with the SLC6A14 proximal promoter, the 2442 bp (2.4 kb) fragment was first generated from human genomic DNA from individuals with CF with appropriate haplotypes by PCR, using SLC6A14-Prom 2442 primers (Table S2) for insertion into the luciferase reporter plasmid pGL4.10 (Promega, E6651) via Acc65I and HindIII sites.



    Similar Products

    noti digested pcmv vector  (TaKaRa)
    93
    TaKaRa noti digested pcmv vector
    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the <t>pCMV</t> control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Noti Digested Pcmv Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/noti+digested+pcmv+vector/pCMV-LacZ+Vector/pmc12769681-100-21-24
    Average 93 stars, based on 1 article reviews
    noti digested pcmv vector - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    ecori noti digested pcmv myc mammalian expression vector  (TaKaRa)
    99
    TaKaRa ecori noti digested pcmv myc mammalian expression vector
    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the <t>pCMV</t> control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Ecori Noti Digested Pcmv Myc Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/noti+digested+pcmv+vector/EcoR+I/10__1074_slash_jbc__m111__308122-62-11-16
    Average 99 stars, based on 1 article reviews
    ecori noti digested pcmv myc mammalian expression vector - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.

    Journal: Human Genetics

    Article Title: Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes

    doi: 10.1007/s00439-025-02800-7

    Figure Lengend Snippet: Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.

    Article Snippet: The SLC6A14 insert was ligated to the EcoRI/BamHI-digested pTetOne vector (Clontech, 634310), and IRF1 , IRF2 inserts were ligated to the NotI-digested pCMV vector (Clontech, 631719), replacing LacZ .

    Techniques: Activity Assay, Luciferase, Plasmid Preparation, Cotransfection, Control