human notch3 extracellular domain  (R&D Systems)

Journal: Signal Transduction and Targeted Therapy

Article Title: Alpha hemolysin enhances the immune response by modulating dendritic cell differentiation via ADAM10-Notch signaling

doi: 10.1038/s41392-025-02432-3

Figure Lengend Snippet: Hla H35A activates Notch signaling via ADAM10 in Fl-BMDCs. a Representative western blot (left) and quantification (right) of N1ICD, N2ICD, Notch3, or Notch4 in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). b Quantification of Hey1 , Hes1 , or Hes5 mRNA expression by qPCR in Fl-BMDCs after 6 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). c Quantification of Adam10 , Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs after 4.5 h of treatment with PBS, PA0833, HPF, or HPF with GI254023X ( n = 3 per group). d TPMs of the Notch1 , Notch2 , Notch3 , or Notch4 genes in unstimulated Fl-BMDCs from bulk RNA-seq ( n = 3 per group) . e Quantification of Notch1 , Notch2 , Notch3 , or Notch4 mRNA expression by qPCR in Fl-BMDCs under homeostatic conditions ( n = 3 per group). Each data point indicates a biological replicate in ( a ) and ( d ). Each data point indicates a biological replicate derived from the average of three technical replicates in ( b , c and e ). The data are presented as the means ± s.e.m.s. Statistical significance was tested via one-way ANOVA followed by Tukey’s multiple comparisons test in ( a – e )

Article Snippet: The membranes were blocked with 5% nonfat milk in TBST (0.1% Tween-20 in TBS) for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against N1ICD (1:1000, CST, Cat# 8216S), N2ICD (1:1000, CST, Cat# 4970T), β-actin (1:1000, CST, Cat# 5732T), Notch3 (1:1000, Santa Cruz, Cat# sc-515825), or Notch4 (1:1000, Santa Cruz, Cat# sc-393893).

Techniques: Western Blot, Expressing, RNA Sequencing, Derivative Assay

Journal: PLOS ONE

Article Title: Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma

doi: 10.1371/journal.pone.0317709

Figure Lengend Snippet: List of primers for detection of Notch pathway components by qRT-PCR.

Article Snippet: DLL4 , Delta like canonical Notch ligand 4 , Ms , Thermo Scientific, Dreieich, Germany , MA5-17069 , 1:200.

Techniques: Marker, Sequencing

Journal: PLOS ONE

Article Title: Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma

doi: 10.1371/journal.pone.0317709

Figure Lengend Snippet: Overview on antibodies used for detection of Notch pathway components by immunofluorescence and PLA.

Article Snippet: DLL4 , Delta like canonical Notch ligand 4 , Ms , Thermo Scientific, Dreieich, Germany , MA5-17069 , 1:200.

Techniques: Immunofluorescence