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normal tissue expression data  (Human Protein Atlas)
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The <t>expression</t> of GDF6 in various human <t>normal</t> <t>tissues</t> and tumor tissues. ( A ) The mRNA expression of GDF6 in normal human tissues. ( B ) GDF6 expression in tumors and healthy tissues (TCGA database). Tumor types with significantly downregulated GDF6 expression ( C ) and those with significantly upregulated expression ( D ) in the TCGA + GTEx databases. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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brain protein expression normal tissue data  (Human Protein Atlas)
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The <t>expression</t> of GDF6 in various human <t>normal</t> <t>tissues</t> and tumor tissues. ( A ) The mRNA expression of GDF6 in normal human tissues. ( B ) GDF6 expression in tumors and healthy tissues (TCGA database). Tumor types with significantly downregulated GDF6 expression ( C ) and those with significantly upregulated expression ( D ) in the TCGA + GTEx databases. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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The expression level of <t>TGIF2</t> in different human cancers and its relationship to glioma prognosis. (A) TGIF2 mRNA expression across various human organs and tissues based on consensus dataset in HPA database. (B) Comparison of TGIF2 expression between normal and tumor tissues across 33 cancers in TCGA and the GTEx database. (C) Subcellular localization of TGIF2 in SH-SY5Y cells from HPA datasets. (D) The volcano plot of DEGs in GSE14805. Red points represent upregulated, blue points represents downregulated genes. (E) ROC curve of TGIF2 and other established prognostic genes (IDH1, IDH2, EGFR, TP53, CDC6, CDC14B, CHD5) in glioma. (F–H) Survival curves for OS (F) , DSS (G) and PFI (H) in gliomas using TCGA database. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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The expression level of <t>TGIF2</t> in different human cancers and its relationship to glioma prognosis. (A) TGIF2 mRNA expression across various human organs and tissues based on consensus dataset in HPA database. (B) Comparison of TGIF2 expression between normal and tumor tissues across 33 cancers in TCGA and the GTEx database. (C) Subcellular localization of TGIF2 in SH-SY5Y cells from HPA datasets. (D) The volcano plot of DEGs in GSE14805. Red points represent upregulated, blue points represents downregulated genes. (E) ROC curve of TGIF2 and other established prognostic genes (IDH1, IDH2, EGFR, TP53, CDC6, CDC14B, CHD5) in glioma. (F–H) Survival curves for OS (F) , DSS (G) and PFI (H) in gliomas using TCGA database. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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The expression level of <t>TGIF2</t> in different human cancers and its relationship to glioma prognosis. (A) TGIF2 mRNA expression across various human organs and tissues based on consensus dataset in HPA database. (B) Comparison of TGIF2 expression between normal and tumor tissues across 33 cancers in TCGA and the GTEx database. (C) Subcellular localization of TGIF2 in SH-SY5Y cells from HPA datasets. (D) The volcano plot of DEGs in GSE14805. Red points represent upregulated, blue points represents downregulated genes. (E) ROC curve of TGIF2 and other established prognostic genes (IDH1, IDH2, EGFR, TP53, CDC6, CDC14B, CHD5) in glioma. (F–H) Survival curves for OS (F) , DSS (G) and PFI (H) in gliomas using TCGA database. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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The expression of GDF6 in various human normal tissues and tumor tissues. ( A ) The mRNA expression of GDF6 in normal human tissues. ( B ) GDF6 expression in tumors and healthy tissues (TCGA database). Tumor types with significantly downregulated GDF6 expression ( C ) and those with significantly upregulated expression ( D ) in the TCGA + GTEx databases. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Journal: Current Issues in Molecular Biology

Article Title: Dual-Faced Role of GDF6 in Cancer: Mechanistic Insights into Its Context-Dependent Regulation of Metastasis and Immune Evasion Across Human Malignancies

doi: 10.3390/cimb47040249

Figure Lengend Snippet: The expression of GDF6 in various human normal tissues and tumor tissues. ( A ) The mRNA expression of GDF6 in normal human tissues. ( B ) GDF6 expression in tumors and healthy tissues (TCGA database). Tumor types with significantly downregulated GDF6 expression ( C ) and those with significantly upregulated expression ( D ) in the TCGA + GTEx databases. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Normal tissue expression data were sourced from the Human Protein Atlas (HPA; https://www.proteinatlas.org , accessed on 15 February 2025).

Techniques: Expressing

The expression level of TGIF2 in different human cancers and its relationship to glioma prognosis. (A) TGIF2 mRNA expression across various human organs and tissues based on consensus dataset in HPA database. (B) Comparison of TGIF2 expression between normal and tumor tissues across 33 cancers in TCGA and the GTEx database. (C) Subcellular localization of TGIF2 in SH-SY5Y cells from HPA datasets. (D) The volcano plot of DEGs in GSE14805. Red points represent upregulated, blue points represents downregulated genes. (E) ROC curve of TGIF2 and other established prognostic genes (IDH1, IDH2, EGFR, TP53, CDC6, CDC14B, CHD5) in glioma. (F–H) Survival curves for OS (F) , DSS (G) and PFI (H) in gliomas using TCGA database. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: The expression level of TGIF2 in different human cancers and its relationship to glioma prognosis. (A) TGIF2 mRNA expression across various human organs and tissues based on consensus dataset in HPA database. (B) Comparison of TGIF2 expression between normal and tumor tissues across 33 cancers in TCGA and the GTEx database. (C) Subcellular localization of TGIF2 in SH-SY5Y cells from HPA datasets. (D) The volcano plot of DEGs in GSE14805. Red points represent upregulated, blue points represents downregulated genes. (E) ROC curve of TGIF2 and other established prognostic genes (IDH1, IDH2, EGFR, TP53, CDC6, CDC14B, CHD5) in glioma. (F–H) Survival curves for OS (F) , DSS (G) and PFI (H) in gliomas using TCGA database. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing, Comparison

Clinical characteristics of glioma patients.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Clinical characteristics of glioma patients.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques:

Logistic analysis of the association between TGIF2 expression and clinical characteristics.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Logistic analysis of the association between TGIF2 expression and clinical characteristics.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Associations between TGIF2 expression and various clinicopathologic characteristics in glioma. (A) Age. (B) IDH status. (C) 1p/19q codeletion status. (D) Primary therapy outcome. (E) OS events. (F) DSS events. (G) PFI events. (H) WHO grade. (I) Histological type. (J) Gender. (K) Race. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns, not significant.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Associations between TGIF2 expression and various clinicopathologic characteristics in glioma. (A) Age. (B) IDH status. (C) 1p/19q codeletion status. (D) Primary therapy outcome. (E) OS events. (F) DSS events. (G) PFI events. (H) WHO grade. (I) Histological type. (J) Gender. (K) Race. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns, not significant.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Prognostic value of TGIF2 expression level in glioma. (A) TGIF2 expression distribution and survival status. (B) Time-dependent ROC curves for TGIF2 expression in glioma. (C) Forest plot of OS by multivariate Cox regression analysis in glioma from TCGA database. (D) The nomogram for predicting 1-, 3-, or 5-year OS rates in patients with glioma. (E) The calibration curves for the nomogram.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Prognostic value of TGIF2 expression level in glioma. (A) TGIF2 expression distribution and survival status. (B) Time-dependent ROC curves for TGIF2 expression in glioma. (C) Forest plot of OS by multivariate Cox regression analysis in glioma from TCGA database. (D) The nomogram for predicting 1-, 3-, or 5-year OS rates in patients with glioma. (E) The calibration curves for the nomogram.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Univariate and multivariate cox regression analyses of clinical characteristics associated with overall survival.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Univariate and multivariate cox regression analyses of clinical characteristics associated with overall survival.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques:

Correlations between TGIF2 expression level and OS in different clinicopathologic subgroups of glioma by Kaplan-Meier survival curve analysis. (A) Age ≤ 60. (B) Age > 60. (C) Gender: Male. (D) Gender: Female. (E) Race: White. (F) WHO grade: G3. (G) 1p/19q codeletion: non-codeletion. (H) Histological type: Astrocytoma. (I) Primary therapy outcome: PD.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Correlations between TGIF2 expression level and OS in different clinicopathologic subgroups of glioma by Kaplan-Meier survival curve analysis. (A) Age ≤ 60. (B) Age > 60. (C) Gender: Male. (D) Gender: Female. (E) Race: White. (F) WHO grade: G3. (G) 1p/19q codeletion: non-codeletion. (H) Histological type: Astrocytoma. (I) Primary therapy outcome: PD.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Functional enrichment analysis of DEGs between TGIF2 high and low expression groups. (A) The volcano plot of DEGs. Red represents upregulated, blue represents downregulated genes. (B) GO and KEGG pathway enrichment analysis of DEGs. (C–H) GSEA functional enrichment analysis.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Functional enrichment analysis of DEGs between TGIF2 high and low expression groups. (A) The volcano plot of DEGs. Red represents upregulated, blue represents downregulated genes. (B) GO and KEGG pathway enrichment analysis of DEGs. (C–H) GSEA functional enrichment analysis.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Functional Assay, Expressing

Correlations between TGIF2 expression and immune cell infiltration in glioma. (A–C) Comparison of StromalScore, ImmuneScore, and EstimateScore between TGIF2 high and low expression groups. (D, E) Comparison of immune cell enrichment scores in high and low TGIF2 expression groups. (F) The lollipop chart showing the correlations between the relative abundances of 24 immune cells and TGIF2 expression levels. (G–N) Scatterplots demonstrating the positive correlation of TGIF2 expression with Th2 cells, macrophages, eosinophils, and neutrophils, and the negative correlation with mast cells, NK CD56bright cells, pDC cells, and TFH cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns, not significant.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Correlations between TGIF2 expression and immune cell infiltration in glioma. (A–C) Comparison of StromalScore, ImmuneScore, and EstimateScore between TGIF2 high and low expression groups. (D, E) Comparison of immune cell enrichment scores in high and low TGIF2 expression groups. (F) The lollipop chart showing the correlations between the relative abundances of 24 immune cells and TGIF2 expression levels. (G–N) Scatterplots demonstrating the positive correlation of TGIF2 expression with Th2 cells, macrophages, eosinophils, and neutrophils, and the negative correlation with mast cells, NK CD56bright cells, pDC cells, and TFH cells. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ns, not significant.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing, Comparison

Analysis of genes coexpressed with TGIF2 in glioma. (A) Heatmap showing the 30 genes in glioma that were 15 positively and 15 negatively related to TGIF2. (B) PPI network of top 50 protein-coding genes positively and negatively correlated with TGIF2. (C) The top 10 hub genes of the PPI network in (B) . (D) The expression levels of the top 10 hub genes in normal and glioma tissues in TCGA and GTEx database. ∗∗∗p < 0.001.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Analysis of genes coexpressed with TGIF2 in glioma. (A) Heatmap showing the 30 genes in glioma that were 15 positively and 15 negatively related to TGIF2. (B) PPI network of top 50 protein-coding genes positively and negatively correlated with TGIF2. (C) The top 10 hub genes of the PPI network in (B) . (D) The expression levels of the top 10 hub genes in normal and glioma tissues in TCGA and GTEx database. ∗∗∗p < 0.001.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Knockdown of TGIF2 inhibits glioma cell invasion, migration and EMT in vitro . (A) Bar graph demonstrating the efficiency of siRNA knockdown of TGIF2 mRNA in U251 cells by RT-qPCR. (B) Western blot assay showing that siRNA effectively knocked down TGIF2 protein, and siTGIF2 downregulated N-cadherin protein expression in U251 cells. (C) Transwell assay demonstrating changes in the number of cells invaded after knockdown of TGIF2. Scale bar, 500 μm. (D) Scratch wound-healing assays were utilized to compare the distance of cell migration between TGIF2-inhibited group and control group at 0h and 24h after scratching. Scale bar, 500 μm. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Knockdown of TGIF2 inhibits glioma cell invasion, migration and EMT in vitro . (A) Bar graph demonstrating the efficiency of siRNA knockdown of TGIF2 mRNA in U251 cells by RT-qPCR. (B) Western blot assay showing that siRNA effectively knocked down TGIF2 protein, and siTGIF2 downregulated N-cadherin protein expression in U251 cells. (C) Transwell assay demonstrating changes in the number of cells invaded after knockdown of TGIF2. Scale bar, 500 μm. (D) Scratch wound-healing assays were utilized to compare the distance of cell migration between TGIF2-inhibited group and control group at 0h and 24h after scratching. Scale bar, 500 μm. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Knockdown, Migration, In Vitro, Quantitative RT-PCR, Western Blot, Expressing, Transwell Assay, Control

Knockout of TGIF2 suppresses glioma cell invasion, migration and EMT. (A) The gRNA sequences designed targeting exon 2 of TGIF2. (B) The editing efficiency of gRNA33 and gRNA140 on TGIF2 was verified in 293T cells utilizing Sanger sequencing. (C) TGIF2-gRNA33 in the UCSC browser. (D) Western blot assay showing the knockout efficiency of TGIF2 protein. WT, Wild type; KO, Knockout. (E) Transwell assays showing changes in the number of cells invaded after knockout of TGIF2. Scale bar, 500 μm. (F) Scratch wound-healing assays were utilized to compare the distance of cell migration between TGIF2-knockout group and control group at 0h and 24h after scratching. Scale bar, 250 μm. ∗p < 0.05.

Journal: Frontiers in Immunology

Article Title: TGIF2 is a potential biomarker for diagnosis and prognosis of glioma

doi: 10.3389/fimmu.2024.1356833

Figure Lengend Snippet: Knockout of TGIF2 suppresses glioma cell invasion, migration and EMT. (A) The gRNA sequences designed targeting exon 2 of TGIF2. (B) The editing efficiency of gRNA33 and gRNA140 on TGIF2 was verified in 293T cells utilizing Sanger sequencing. (C) TGIF2-gRNA33 in the UCSC browser. (D) Western blot assay showing the knockout efficiency of TGIF2 protein. WT, Wild type; KO, Knockout. (E) Transwell assays showing changes in the number of cells invaded after knockout of TGIF2. Scale bar, 500 μm. (F) Scratch wound-healing assays were utilized to compare the distance of cell migration between TGIF2-knockout group and control group at 0h and 24h after scratching. Scale bar, 250 μm. ∗p < 0.05.

Article Snippet: The mRNA expression data of TGIF2 in normal tissues and subcellular localization of TGIF2 protein in SH-SY5Y cell line were sourced from Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Knock-Out, Migration, Sequencing, Western Blot, Control