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beas 2b immortalized normal lung epithelial cells  (ATCC)


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    ATCC beas 2b immortalized normal lung epithelial cells
    Beas 2b Immortalized Normal Lung Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+lung+epithelial+cells/BEAS-2B/pm42210336-53-13-22
    Average 99 stars, based on 4041 article reviews
    beas 2b immortalized normal lung epithelial cells - by Bioz Stars, 2026-10
    99/100 stars

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    In Vitro:

    Article Title: BamA Targeted Design of Antimicrobial Peptides with High Efficacy and Low Toxicity
    Article Snippet: .. Page 12/25 Peptide MIC (μg/mL) 0% 5% 10% 11pep 64 128 128 D-11pep 16 16 32 3.11 Cell viability of antimicrobial peptides Cytotoxicity of 11pep and D-11pep on human normal lung epithelial cells (BEAS-2B cells ATCC CRL-9609) were assessed in vitro. ..

    Cell Culture:

    Article Title: Inhibition of Lung Cancer Stem Cell Migration and Growth Through Nano-Photodynamic Therapy
    Article Snippet: .. Normal lung epithelial cells (HBEC3-KT (ATCC® CRL-4051TM)) were cultured in Airway Epithelial Cell Basal Medium (ATCC PCS-300-030) and Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040). .. Cancer cells were cultured in Rosewell Park Memorial Institute 1640 medium (RPMI-1640) (Sigma-Aldrich, R8758) supplemented with 5% fetal bovine serum (FBS) (Biochrom, S0615) and 0.5% penicillin/ streptomycin (Sigma-Aldrich, P4333) and 0.5% amphotericin B (Sigma-Aldrich, A2942).

    Article Title: CDC6 may serve as an indicator of lung adenocarcinoma prognosis and progression based on TCGA and GEO data mining and experimental analyses
    Article Snippet: .. Cells, including human normal lung epithelial cells [BEAS-2B; American Type Culture Collection (ATCC)], human lung cancer cells (A549; ATCC), human LUAD cells (NCI-H1975; ATCC), mouse lung cancer cells (Lewis) and 293T cells (Procell Life Technology Co., Ltd.), were cultured in RPMI-1640 medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (all Gibco; Thermo Fisher Scientific, Inc.). ..

    Article Title: CDC6 may serve as an indicator of lung adenocarcinoma prognosis and progression based on TCGA and GEO data mining and experimental analyses.
    Article Snippet: .. Cells, including human normal lung epithelial cells [BEAS‐2B; American Type Culture Collection (ATCC)], human lung cancer cells (A549; ATCC), human LUAD cells (NCI‐H1975; ATCC), mouse lung cancer cells (Lewis) and 293T cells (Procell Life Technology Co., Ltd.), were cultured in RPMI‐1640 medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (all Gibco; Thermo Fisher Scientific, Inc.). ..

    Derivative Assay:

    Article Title: Roles for Spatiotemporal Intracellular pH Dynamics in Single-Cell Phenotypes and Behaviors
    Article Snippet: 2.3 Methods 2.3.1 Cell culture and conditions Complete media for metastatic lung cancer cells (H1299 (ATCC CRL-5803)]: RPMI 1640 (Corning, 10-040-CV) supplemented with 10% Fetal Bovine Serum (FBS, 7 .. Peak Serum, PS-FB2); primary-site derived lung cancer cells [A549 ATCC CCL-185)] and metastatic breast cancer cells [MDA-MB-231 (ATCC HTB-26)]: DMEM (Corning, MT10013CVV) supplemented with 10% FBS; and normal lung epithelial cells [NL20 (ATCC CRL-2503)]: Ham’s F12 (Lonza, 12001-578) supplemented with 4% FBS, 1.5 g/L sodium bicarbonate (Sigma, $6014), 2.7 g/L glucose (VWR, BDH9230), 2.0 mM GlutaMAX (Gibco, 35050079), 0.1 mM nonessential amino acids (Lonza, BW13114E), 0.005 mg/mL insulin (Sigma, 11882), 10 ng/mL EGF (Peprotech, AF-100-15), 0.001 mg/mL transferrin (BioVision, 10835-642), 0.5 ng/mL hydrocortisone (Sigma, H0888). .. Normal breast epithelial cells [MCF10A (ATCC CRL-10317)]: DMEM/50% F12 w/ GlutaMAX (Invitrogen, 10565-018) supplemented with 5% Horse Serum (Invitrogen, 16050-122), 0.02 ug/mL EGF (Peprotech, AF-100-15), 5 g/mL Hydrocortisone (Sigma, H-0888), 10 mg/mL Insulin (Sigma, I-1882), 0.1 ug/mL Cholera toxin (Sigma, C-8052), and 1% Penicillin- Streptomycin (Corning, 30-001-Cl).

    Multiple Displacement Amplification:

    Article Title: Roles for Spatiotemporal Intracellular pH Dynamics in Single-Cell Phenotypes and Behaviors
    Article Snippet: 2.3 Methods 2.3.1 Cell culture and conditions Complete media for metastatic lung cancer cells (H1299 (ATCC CRL-5803)]: RPMI 1640 (Corning, 10-040-CV) supplemented with 10% Fetal Bovine Serum (FBS, 7 .. Peak Serum, PS-FB2); primary-site derived lung cancer cells [A549 ATCC CCL-185)] and metastatic breast cancer cells [MDA-MB-231 (ATCC HTB-26)]: DMEM (Corning, MT10013CVV) supplemented with 10% FBS; and normal lung epithelial cells [NL20 (ATCC CRL-2503)]: Ham’s F12 (Lonza, 12001-578) supplemented with 4% FBS, 1.5 g/L sodium bicarbonate (Sigma, $6014), 2.7 g/L glucose (VWR, BDH9230), 2.0 mM GlutaMAX (Gibco, 35050079), 0.1 mM nonessential amino acids (Lonza, BW13114E), 0.005 mg/mL insulin (Sigma, 11882), 10 ng/mL EGF (Peprotech, AF-100-15), 0.001 mg/mL transferrin (BioVision, 10835-642), 0.5 ng/mL hydrocortisone (Sigma, H0888). .. Normal breast epithelial cells [MCF10A (ATCC CRL-10317)]: DMEM/50% F12 w/ GlutaMAX (Invitrogen, 10565-018) supplemented with 5% Horse Serum (Invitrogen, 16050-122), 0.02 ug/mL EGF (Peprotech, AF-100-15), 5 g/mL Hydrocortisone (Sigma, H-0888), 10 mg/mL Insulin (Sigma, I-1882), 0.1 ug/mL Cholera toxin (Sigma, C-8052), and 1% Penicillin- Streptomycin (Corning, 30-001-Cl).



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    THOC3 expression is upregulated in LC and is associated with unfavorable prognoses. (A, B) The mRNA expression of THOC3 in LUAD and LUSC tissues, as well as normal lung tissues, was obtained from the GEPIA database. (C) Real-time PCR analysis of THOC3 mRNA expression in cancerous and adjacent non-cancerous tissues from LC patients. (D) Western blot analysis of THOC3 protein levels in cancerous and adjacent tissues from LC patients. (E) Real-time PCR analysis of THOC3 mRNA expression in LC cell lines (H1975, A549, H1299, and PC9) and the normal bronchial epithelial cell line BEAS-2B (F) Western blot analysis of THOC3 protein expression in LC cell lines (H1975, A549, H1299, and PC9) and BEAS-2B cells. (G) Real-time PCR analysis of THOC3 mRNA expression in normal lung tissues and LC samples from different clinical grades. (H) Western blot analysis of THOC3 protein levels in normal lung tissues and LC samples from various clinical stages. (I, J, K) Kaplan-Meier survival curves illustrating the overall survival (OS) of LC patients with high or low THOC3 expression from the TCGA database. (L) Kaplan-Meier survival curves depicting disease-free survival (DFS) in high vs. low THOC3-expressing LC patients from the TCGA database. Each experiment was performed in triplicate. Data are expressed as mean ± SEM; * P < 0.05.

    Journal: Translational Oncology

    Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway

    doi: 10.1016/j.tranon.2026.102741

    Figure Lengend Snippet: THOC3 expression is upregulated in LC and is associated with unfavorable prognoses. (A, B) The mRNA expression of THOC3 in LUAD and LUSC tissues, as well as normal lung tissues, was obtained from the GEPIA database. (C) Real-time PCR analysis of THOC3 mRNA expression in cancerous and adjacent non-cancerous tissues from LC patients. (D) Western blot analysis of THOC3 protein levels in cancerous and adjacent tissues from LC patients. (E) Real-time PCR analysis of THOC3 mRNA expression in LC cell lines (H1975, A549, H1299, and PC9) and the normal bronchial epithelial cell line BEAS-2B (F) Western blot analysis of THOC3 protein expression in LC cell lines (H1975, A549, H1299, and PC9) and BEAS-2B cells. (G) Real-time PCR analysis of THOC3 mRNA expression in normal lung tissues and LC samples from different clinical grades. (H) Western blot analysis of THOC3 protein levels in normal lung tissues and LC samples from various clinical stages. (I, J, K) Kaplan-Meier survival curves illustrating the overall survival (OS) of LC patients with high or low THOC3 expression from the TCGA database. (L) Kaplan-Meier survival curves depicting disease-free survival (DFS) in high vs. low THOC3-expressing LC patients from the TCGA database. Each experiment was performed in triplicate. Data are expressed as mean ± SEM; * P < 0.05.

    Article Snippet: The human normal lung epithelial cell line BEAS-2B was obtained from the American Type Culture Collection.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Acidic microenvironment condition screening and LAMC2 expression validation. (A) Comparison of relative LAMC2 expression levels in different cell lines (BEAS-2B, HCC827, and A549), showing a significantly higher expression in HCC827 and A549 cells compared to BEAS-2B cells. (B) Changes in LAMC2 expression in HCC827 and A549 cells under different lactate (LA) concentrations (10, 20, and 30 mM), showing significant increases in LAMC2 expression at 20 and 30 mM concentrations. (C) Changes in LAMC2 expression over time (0, 6, 12, and 24 h) in HCC827 and A549 cells treated with 20 mM LA, showing a significant increase in expression with longer treatment times. (D) Changes in LAMC2 expression under different acidic environment conditions (high LA, hydrochloric-acid-induced acidosis, and control groups) in HCC827 and A549 cells, with a significantly higher expression in the high LA and hydrochloric-acid-induced acidosis groups. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Oncology

    Article Title: Acidic microenvironment-induced LAMC2 expression promotes proliferation, migration, and pathway regulation in non-small cell lung cancer cells

    doi: 10.3389/fonc.2026.1747868

    Figure Lengend Snippet: Acidic microenvironment condition screening and LAMC2 expression validation. (A) Comparison of relative LAMC2 expression levels in different cell lines (BEAS-2B, HCC827, and A549), showing a significantly higher expression in HCC827 and A549 cells compared to BEAS-2B cells. (B) Changes in LAMC2 expression in HCC827 and A549 cells under different lactate (LA) concentrations (10, 20, and 30 mM), showing significant increases in LAMC2 expression at 20 and 30 mM concentrations. (C) Changes in LAMC2 expression over time (0, 6, 12, and 24 h) in HCC827 and A549 cells treated with 20 mM LA, showing a significant increase in expression with longer treatment times. (D) Changes in LAMC2 expression under different acidic environment conditions (high LA, hydrochloric-acid-induced acidosis, and control groups) in HCC827 and A549 cells, with a significantly higher expression in the high LA and hydrochloric-acid-induced acidosis groups. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Human normal lung epithelial BEAS-2B cells (ATCC ® CRL-9609TM) and lung cancer cell lines A549 (Procell, CL-0016, lung adenocarcinoma) and HCC827 (Procell, CL-0094, lung adenocarcinoma) were obtained from the indicated suppliers.

    Techniques: Expressing, Biomarker Discovery, Comparison, Control