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human normal small intestinal epithelial cells  (ATCC)


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    ATCC human normal small intestinal epithelial cells
    Human Normal Small Intestinal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+intestinal/HIEC-6/pmc12989582-78-0-7
    Average 96 stars, based on 235 article reviews
    human normal small intestinal epithelial cells - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: New Compounds with Enhanced Biological Activity Through the Strategic Introduction of Silylated Groups into Hydroxystearic Acids
    Article Snippet: The human colorectal adenocarcinoma (Caco-2), human colorectal adenocarcinoma (HT29), normal human intestinal (I407), human cervical cancer (HeLa), human breast cancer (MCF7), human bone osteosarcoma (U2OS), and human acute T-cell leukemia (Jurkat J6) cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).

    Cell Culture:

    Article Title: New Compounds with Enhanced Biological Activity Through the Strategic Introduction of Silylated Groups into Hydroxystearic Acids
    Article Snippet: .. Cell Culture and Treatments The human colorectal adenocarcinoma (Caco-2), human colorectal adenocarcinoma (HT29), normal human intestinal (I407), human cervical cancer (HeLa), human breast cancer (MCF7), human bone osteosarcoma (U2OS), and human acute T-cell leukemia (Jurkat J6) cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). .. Cells were cultured in RPMI 1640 medium (Labtek Eurobio, Milan, Italy), supplemented with 10% FCS (Euroclone, Milano, Italy) and 2 mM L-glutamine (Sigma-Aldrich, Milano, Italy), at 37 ◦C and in a 5% CO2 atmosphere.



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    Experimental validation of the eight-gene panel and PLK1 manipulation in colorectal cell models. A qRT–PCR of ASPM, AURKA, CDCA8, CENPF, KIF23, NUF2, PLK1, TOP2A in SW620, Caco-2, and <t>HIEC-6.</t> B qRT–PCR measurement of PLK1 after transfection with si-PLK1#1/#2 or siNC in SW620 and Caco-2; si-PLK1#1 used for subsequent assays. C Wound-healing assay (0 and 48 h) in SW620 and Caco-2; representative images and quantification of wound width. D Matrigel transwell invasion of SW620 and Caco-2 (48 h); representative micrographs and quantification (cells/field). E CCK-8 time-course curves at 0, 24, 48, 72 h for SW620 and Caco-2; OD 450 values plotted. All procedures were performed in triplicate ( n = 3). F Flow cytometry was used to assess the effect of PLK1 silencing on the apoptotic capacity of cancer cells. *Statistical significance is indicated as follows: * P < 0.05, *** P < 0.001, **** P < 0.0001
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    Experimental validation of the eight-gene panel and PLK1 manipulation in colorectal cell models. A qRT–PCR of ASPM, AURKA, CDCA8, CENPF, KIF23, NUF2, PLK1, TOP2A in SW620, Caco-2, and <t>HIEC-6.</t> B qRT–PCR measurement of PLK1 after transfection with si-PLK1#1/#2 or siNC in SW620 and Caco-2; si-PLK1#1 used for subsequent assays. C Wound-healing assay (0 and 48 h) in SW620 and Caco-2; representative images and quantification of wound width. D Matrigel transwell invasion of SW620 and Caco-2 (48 h); representative micrographs and quantification (cells/field). E CCK-8 time-course curves at 0, 24, 48, 72 h for SW620 and Caco-2; OD 450 values plotted. All procedures were performed in triplicate ( n = 3). F Flow cytometry was used to assess the effect of PLK1 silencing on the apoptotic capacity of cancer cells. *Statistical significance is indicated as follows: * P < 0.05, *** P < 0.001, **** P < 0.0001
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    Experimental validation of the eight-gene panel and PLK1 manipulation in colorectal cell models. A qRT–PCR of ASPM, AURKA, CDCA8, CENPF, KIF23, NUF2, PLK1, TOP2A in SW620, Caco-2, and <t>HIEC-6.</t> B qRT–PCR measurement of PLK1 after transfection with si-PLK1#1/#2 or siNC in SW620 and Caco-2; si-PLK1#1 used for subsequent assays. C Wound-healing assay (0 and 48 h) in SW620 and Caco-2; representative images and quantification of wound width. D Matrigel transwell invasion of SW620 and Caco-2 (48 h); representative micrographs and quantification (cells/field). E CCK-8 time-course curves at 0, 24, 48, 72 h for SW620 and Caco-2; OD 450 values plotted. All procedures were performed in triplicate ( n = 3). F Flow cytometry was used to assess the effect of PLK1 silencing on the apoptotic capacity of cancer cells. *Statistical significance is indicated as follows: * P < 0.05, *** P < 0.001, **** P < 0.0001
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    Experimental validation of the eight-gene panel and PLK1 manipulation in colorectal cell models. A qRT–PCR of ASPM, AURKA, CDCA8, CENPF, KIF23, NUF2, PLK1, TOP2A in SW620, Caco-2, and HIEC-6. B qRT–PCR measurement of PLK1 after transfection with si-PLK1#1/#2 or siNC in SW620 and Caco-2; si-PLK1#1 used for subsequent assays. C Wound-healing assay (0 and 48 h) in SW620 and Caco-2; representative images and quantification of wound width. D Matrigel transwell invasion of SW620 and Caco-2 (48 h); representative micrographs and quantification (cells/field). E CCK-8 time-course curves at 0, 24, 48, 72 h for SW620 and Caco-2; OD 450 values plotted. All procedures were performed in triplicate ( n = 3). F Flow cytometry was used to assess the effect of PLK1 silencing on the apoptotic capacity of cancer cells. *Statistical significance is indicated as follows: * P < 0.05, *** P < 0.001, **** P < 0.0001

    Journal: European Journal of Medical Research

    Article Title: A core stemness-associated module reveals PLK1, NUF2, KIF23, CDCA8, TOP2A, CENPF, AURKA, and ASPM as key genes in rectal cancer

    doi: 10.1186/s40001-025-03649-2

    Figure Lengend Snippet: Experimental validation of the eight-gene panel and PLK1 manipulation in colorectal cell models. A qRT–PCR of ASPM, AURKA, CDCA8, CENPF, KIF23, NUF2, PLK1, TOP2A in SW620, Caco-2, and HIEC-6. B qRT–PCR measurement of PLK1 after transfection with si-PLK1#1/#2 or siNC in SW620 and Caco-2; si-PLK1#1 used for subsequent assays. C Wound-healing assay (0 and 48 h) in SW620 and Caco-2; representative images and quantification of wound width. D Matrigel transwell invasion of SW620 and Caco-2 (48 h); representative micrographs and quantification (cells/field). E CCK-8 time-course curves at 0, 24, 48, 72 h for SW620 and Caco-2; OD 450 values plotted. All procedures were performed in triplicate ( n = 3). F Flow cytometry was used to assess the effect of PLK1 silencing on the apoptotic capacity of cancer cells. *Statistical significance is indicated as follows: * P < 0.05, *** P < 0.001, **** P < 0.0001

    Article Snippet: Human normal intestinal epithelial cells HIEC-6 (CRL-3266, ATCC, Manassas, VA, USA) and human colorectal cancer cell lines SW620 (TCHu101, National Collection of Authenticated Cell Cultures/Shanghai Cell Bank, Shanghai, China) and Caco-2 (SCSP-5027, Shanghai Cell Bank, Shanghai, China) were used.

    Techniques: Biomarker Discovery, Quantitative RT-PCR, Transfection, Wound Healing Assay, CCK-8 Assay, Flow Cytometry