noncontact microarray printer (SCIENION)

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SCIENION noncontact microarray printer

<t>Microarray</t> analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

Noncontact Microarray Printer, supplied by SCIENION, used in various techniques. Bioz Stars score: 96/100, based on 317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/noncontact+microarray+printer/sciFLEXARRAYER+S3/pmc12175471-139-7-12
Average 96 stars, based on 317 article reviews

noncontact microarray printer - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection"

Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection

Journal: Journal of Neuroinflammation

doi: 10.1186/s12974-025-03482-8

Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

Figure Legend Snippet: Microarray analysis reveals the effects of GFAP mAb treatment on key inflammatory mediators in glaucomatous retina. ( A - C ) The expression of TLR4 and S100A8 was significantly downregulated by 25–50 µg GFAP mAb treatment compared to the vehicle. The marker for microglial activation, CD68, was significantly decreased in the 25 µg GFAP mAb treatment group. ( D - F ) Proteins of the inflammasome pathway, NLRP3, GSDMD and Caspase-1 were significantly downregulated in the 25 µg GFAP mAb treatment group. With the 50 µg dose, these protein expressions showed a similar downward trend, but without statistical significance. ( G ) Expression profiling of inflammation-associated mediators showed that 25 µg GFAP mAb significantly decreased pro-inflammatory factors (TNF-α, IL-1β, IL-8, MMP9, and IFN-γ), and increased the anti-inflammatory cytokine IL-10 compared to the vehicle group. In contrast, the 50 µg dose significantly reduced IFN-γ only. ( H ) Representative images of spots showing IL-1β levels in the subarrays for each group. Image analysis was conducted with Imagene software, using the median intensity from each spot to calculate the mean of the triplicate spots for each marker. For CD68 and TLR4, due to significant heterogeneity in SDs, statistical analysis was performed using Welch’s ANOVA followed by the Tamhane T2 post hoc test. All other data were analyzed using one-way ANOVA with Tukey’s post hoc test. Data are presented as the mean ± SD; n = 4 per group; * p < 0.05, ** p < 0.01, *** p < 0.001; ns = not significant. TLR4 – Toll-Like Receptor 4. S100A8 – S100 Calcium Binding Protein A8. NLRP3 – NLR Family Pyrin Domain Containing 3. GSDMD – Gasdermin D

Techniques Used: Microarray, Expressing, Marker, Activation Assay, Software, Binding Assay

Microarray:

Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection.
Article Snippet: .. Briefly, the microarray was prepared using a noncontact microarray printer (SciFLEXARRAYER S3; Scienion, Berlin, Germany). .. Antibodies (supplementary Table 2) were spotted in triplicate onto nitrocellulosecoated slides (AVID Oncyte, NC 16 Pad slides; Grace Bio-Labs, Bend, OR, USA).

Article Title: Neuroretinal Cell Culture Model as a Tool for the Development of New Therapeutic Approaches for Oxidative Stress-Induced Ocular Diseases, with a Focus on Glaucoma
Article Snippet: In addition, the functional annotation and the signalling pathway analyses were carried out using Ingenuity Pathway Analysis software v. 1-04 (IPA, Ingenuity QIAGEN; Redwood City, CA, USA) [ , ]. .. Microarray manufacturing was performed in our lab using a noncontact microarray printer (SciFLEXARRAYER S3; Scienion, Berlin, Germany). .. The HTRA2 antibodies (Proteintech GmbH; Manchester, UK) were spotted onto nitrocellulose-coated microarray slides (AVID Oncyte, NC 16 Pad slides; Grace Bio-Labs, Bend, OR, USA) in triplicate.

Article Title: Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection
Article Snippet: .. Briefly, the microarray was prepared using a noncontact microarray printer (SciFLEXARRAYER S3; Scienion, Berlin, Germany). .. Antibodies (supplementary Table ) were spotted in triplicate onto nitrocellulose-coated slides (AVID Oncyte, NC 16 Pad slides; Grace Bio-Labs, Bend, OR, USA).

Article Title: Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties
Article Snippet: .. Microarray-based indirect sandwich immunoassays (ISIAs) against anti-HIV p24 Abs on POEGMA. (A) anti-APA ISIA: Microarrays of PEGylated BSA (Life Diagnostics PBSA-00) were prepared by printing onto surfaces with a noncontact microarray printer (Scienion sciFlEXARRAYER S11) at a concentration of 1 mg/mL as microspots and then placed in a vacuum dessicator overnight. .. Next, these assays were run against serial dilutions of pAPA1 (ThermoFisher PA5–32247) spiked into calf serum and incubated for 1 h. The surfaces were then washed 3 times and then exposed to a 1 μg/mL solution of Cy5-donkey-anti-rabbit antibody (R&D Systems) for 30 min.

Article Title: Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties
Article Snippet: .. (A) anti-APA ISIA: Microarrays of PEGylated BSA (Life Diagnostics PBSA-00) were prepared by printing onto surfaces with a noncontact microarray printer (Scienion sciFlEXARRAYER S11) at a concentration of 1 mg/mL as microspots and then placed in a vacuum dessicator overnight. .. Next, these assays were run against serial dilutions of pAPA1 (ThermoFisher PA5–32247) spiked into calf serum and incubated for 1 h. The surfaces were then washed 3 times and then exposed to a 1 μg/mL solution of Cy5-donkey-anti-rabbit antibody (R&D Systems) for 30 min.

Concentration Assay:

Article Title: Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties
Article Snippet: .. (A) anti-APA ISIA: Microarrays of PEGylated BSA (Life Diagnostics PBSA-00) were prepared by printing onto surfaces with a noncontact microarray printer (Scienion sciFlEXARRAYER S11) at a concentration of 1 mg/mL as microspots and then placed in a vacuum dessicator overnight. .. Next, these assays were run against serial dilutions of pAPA1 (ThermoFisher PA5–32247) spiked into calf serum and incubated for 1 h. The surfaces were then washed 3 times and then exposed to a 1 μg/mL solution of Cy5-donkey-anti-rabbit antibody (R&D Systems) for 30 min.

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noncontact microarray printer (SCIENION)

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1) Product Images from "Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection"

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