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    ATCC non type strain
    Non Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+type+strains/Ruminococcus+torques+Holdeman+and+Moore/pm42126677-113-30-34
    Average 94 stars, based on 5 article reviews
    non type strain - by Bioz Stars, 2026-09
    94/100 stars

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    Derivative Assay:

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no. a : 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 77.4 100 3 A. niger ATCC 1015 85.0 79.5 100 4 A. terreus ATCC 1012 84.0 78.0 87.8 100 5 A. flavipes ATCC 24487 79.5 75.3 82.3 86.8 100 6 A. candidus NRRL 303 84.0 82.3 82.4 79.8 78.7 100 7 A. restrictus NRRL 148 83.1 75.0 83.0 80.4 77.4 78.9 100 8 A. chevalieri ATCC 16443 63.8 60.0 62.6 60.4 57.8 61.5 71.6 100 9 A. granulosus NRRL 1932 58.9 61.2 58.2 58.5 60.6 60.1 57.4 63.8 100 10 A. ustus NRRL 275 59.0 60.4 59.2 60.5 58.7 58.7 59.2 60.1 92.5 100 11 A. sydowii NRRL 254 62.6 61.8 60.7 59.5 59.5 63.2 61.7 68.5 73.9 72.6 100 12 A. versicolor NRRL 238 62.6 61.3 62.3 60.5 60.1 62.3 61.7 69.8 73.3 70.7 98.1 100 13 A. nidulans ATCC 10074 63.7 60.4 61.3 60.0 60.4 62.1 61.1 67.5 74.8 75.0 94.2 94.8 100 Open in a separate window a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table ) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains ( A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data ( 15 ). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no. a : 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 94.3 100 3 A. niger ATCC 1015 94.6 96.7 100 4 A. terreus ATCC 1012 95.6 95.2 95.8 100 5 A. flavipes ATCC 24487 95.9 96.5 95.8 96.5 100 6 A. candidus NRRL 303 95.6 96.9 96.1 95.6 96.7 100 7 A. restrictus NRRL 148 93.4 92.8 93.4 93.5 93.4 93.2 100 8 A. chevalieri ATCC 16443 94.5 94.5 94.5 94.6 95.0 94.6 95.8 100 9 A. granulosus NRRL 1932 93.9 94.5 95.2 93.4 95.0 93.9 93.0 94.3 100 10 A. ustus NRRL 275 93.5 94.1 94.8 93.0 94.6 93.5 92.6 93.9 99.6 100 11 A. sydowii NRRL 254 93.2 93.5 94.5 93.0 94.1 93.2 91.9 93.4 98.3 98.0 100 12 A. versicolor NRRL 238 93.4 93.7 94.6 92.8 94.3 93.4 92.1 93.5 98.5 98.5 99.1 100 13 A. nidulans ATCC 10074 94.5 94.7 95.4 93.6 94.8 94.1 92.8 93.4 98.7 98.7 97.4 97.6 100 Open in a separate window a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table ) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains ( A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data ( 35 ). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no.a: 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 87.7 100 3 A. niger ATCC 1015 87.1 87.2 100 4 A. terreus ATCC 1012 85.9 83.3 83.6 100 5 A. flavipes ATCC 24487 87.5 85.9 83.4 83.6 100 6 A. candidus NRRL 303 89.4 86.0 85.9 84.2 86.3 100 7 A. restrictus NRRL 148 81.5 80.0 79.5 75.6 78.0 83.3 100 8 A. chevalieri ATCC 16443 80.1 80.9 75.9 76.4 76.6 82.5 83.2 100 9 A. granulosus NRRL 1932 84.6 83.4 84.7 83.5 86.6 85.2 78.1 79.2 100 10 A. ustus NRRL 275 85.3 83.6 84.1 82.3 85.5 84.2 78.8 78.7 98.3 100 11 A. sydowii NRRL 254 85.1 83.8 85.5 82.2 87.0 86.1 78.5 78.8 94.2 91.9 100 12 A. versicolor NRRL 238 86.7 84.9 85.6 81.8 88.2 86.2 79.0 78.8 93.6 91.9 97.0 100 13 A. nidulans ATCC 10074 87.2 82.1 85.0 82.7 86.9 85.0 79.5 79.4 95.3 94.8 95.9 95.3 100 a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table 1) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains (A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data (15). on N ovem ber 5, 2012 by U N IV O F S U S S E X http://jcm .asm .org/ D ow nloaded from occurred over 209 aligned positions) (Fig. 1). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no.a: 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 77.4 100 3 A. niger ATCC 1015 85.0 79.5 100 4 A. terreus ATCC 1012 84.0 78.0 87.8 100 5 A. flavipes ATCC 24487 79.5 75.3 82.3 86.8 100 6 A. candidus NRRL 303 84.0 82.3 82.4 79.8 78.7 100 7 A. restrictus NRRL 148 83.1 75.0 83.0 80.4 77.4 78.9 100 8 A. chevalieri ATCC 16443 63.8 60.0 62.6 60.4 57.8 61.5 71.6 100 9 A. granulosus NRRL 1932 58.9 61.2 58.2 58.5 60.6 60.1 57.4 63.8 100 10 A. ustus NRRL 275 59.0 60.4 59.2 60.5 58.7 58.7 59.2 60.1 92.5 100 11 A. sydowii NRRL 254 62.6 61.8 60.7 59.5 59.5 63.2 61.7 68.5 73.9 72.6 100 12 A. versicolor NRRL 238 62.6 61.3 62.3 60.5 60.1 62.3 61.7 69.8 73.3 70.7 98.1 100 13 A. nidulans ATCC 10074 63.7 60.4 61.3 60.0 60.4 62.1 61.1 67.5 74.8 75.0 94.2 94.8 100 a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table 1) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains (A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data (15). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no.a: 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 94.3 100 3 A. niger ATCC 1015 94.6 96.7 100 4 A. terreus ATCC 1012 95.6 95.2 95.8 100 5 A. flavipes ATCC 24487 95.9 96.5 95.8 96.5 100 6 A. candidus NRRL 303 95.6 96.9 96.1 95.6 96.7 100 7 A. restrictus NRRL 148 93.4 92.8 93.4 93.5 93.4 93.2 100 8 A. chevalieri ATCC 16443 94.5 94.5 94.5 94.6 95.0 94.6 95.8 100 9 A. granulosus NRRL 1932 93.9 94.5 95.2 93.4 95.0 93.9 93.0 94.3 100 10 A. ustus NRRL 275 93.5 94.1 94.8 93.0 94.6 93.5 92.6 93.9 99.6 100 11 A. sydowii NRRL 254 93.2 93.5 94.5 93.0 94.1 93.2 91.9 93.4 98.3 98.0 100 12 A. versicolor NRRL 238 93.4 93.7 94.6 92.8 94.3 93.4 92.1 93.5 98.5 98.5 99.1 100 13 A. nidulans ATCC 10074 94.5 94.7 95.4 93.6 94.8 94.1 92.8 93.4 98.7 98.7 97.4 97.6 100 a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table 1) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains (A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data (35). on N ovem ber 5, 2012 by U N IV O F S U S S E X http://jcm .asm .org/ D ow nloaded from (Table 3). ..

    Sequencing:

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no. a : 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 77.4 100 3 A. niger ATCC 1015 85.0 79.5 100 4 A. terreus ATCC 1012 84.0 78.0 87.8 100 5 A. flavipes ATCC 24487 79.5 75.3 82.3 86.8 100 6 A. candidus NRRL 303 84.0 82.3 82.4 79.8 78.7 100 7 A. restrictus NRRL 148 83.1 75.0 83.0 80.4 77.4 78.9 100 8 A. chevalieri ATCC 16443 63.8 60.0 62.6 60.4 57.8 61.5 71.6 100 9 A. granulosus NRRL 1932 58.9 61.2 58.2 58.5 60.6 60.1 57.4 63.8 100 10 A. ustus NRRL 275 59.0 60.4 59.2 60.5 58.7 58.7 59.2 60.1 92.5 100 11 A. sydowii NRRL 254 62.6 61.8 60.7 59.5 59.5 63.2 61.7 68.5 73.9 72.6 100 12 A. versicolor NRRL 238 62.6 61.3 62.3 60.5 60.1 62.3 61.7 69.8 73.3 70.7 98.1 100 13 A. nidulans ATCC 10074 63.7 60.4 61.3 60.0 60.4 62.1 61.1 67.5 74.8 75.0 94.2 94.8 100 Open in a separate window a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table ) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains ( A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data ( 15 ). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no. a : 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 94.3 100 3 A. niger ATCC 1015 94.6 96.7 100 4 A. terreus ATCC 1012 95.6 95.2 95.8 100 5 A. flavipes ATCC 24487 95.9 96.5 95.8 96.5 100 6 A. candidus NRRL 303 95.6 96.9 96.1 95.6 96.7 100 7 A. restrictus NRRL 148 93.4 92.8 93.4 93.5 93.4 93.2 100 8 A. chevalieri ATCC 16443 94.5 94.5 94.5 94.6 95.0 94.6 95.8 100 9 A. granulosus NRRL 1932 93.9 94.5 95.2 93.4 95.0 93.9 93.0 94.3 100 10 A. ustus NRRL 275 93.5 94.1 94.8 93.0 94.6 93.5 92.6 93.9 99.6 100 11 A. sydowii NRRL 254 93.2 93.5 94.5 93.0 94.1 93.2 91.9 93.4 98.3 98.0 100 12 A. versicolor NRRL 238 93.4 93.7 94.6 92.8 94.3 93.4 92.1 93.5 98.5 98.5 99.1 100 13 A. nidulans ATCC 10074 94.5 94.7 95.4 93.6 94.8 94.1 92.8 93.4 98.7 98.7 97.4 97.6 100 Open in a separate window a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table ) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains ( A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data ( 35 ). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no.a: 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 87.7 100 3 A. niger ATCC 1015 87.1 87.2 100 4 A. terreus ATCC 1012 85.9 83.3 83.6 100 5 A. flavipes ATCC 24487 87.5 85.9 83.4 83.6 100 6 A. candidus NRRL 303 89.4 86.0 85.9 84.2 86.3 100 7 A. restrictus NRRL 148 81.5 80.0 79.5 75.6 78.0 83.3 100 8 A. chevalieri ATCC 16443 80.1 80.9 75.9 76.4 76.6 82.5 83.2 100 9 A. granulosus NRRL 1932 84.6 83.4 84.7 83.5 86.6 85.2 78.1 79.2 100 10 A. ustus NRRL 275 85.3 83.6 84.1 82.3 85.5 84.2 78.8 78.7 98.3 100 11 A. sydowii NRRL 254 85.1 83.8 85.5 82.2 87.0 86.1 78.5 78.8 94.2 91.9 100 12 A. versicolor NRRL 238 86.7 84.9 85.6 81.8 88.2 86.2 79.0 78.8 93.6 91.9 97.0 100 13 A. nidulans ATCC 10074 87.2 82.1 85.0 82.7 86.9 85.0 79.5 79.4 95.3 94.8 95.9 95.3 100 a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table 1) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains (A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data (15). on N ovem ber 5, 2012 by U N IV O F S U S S E X http://jcm .asm .org/ D ow nloaded from occurred over 209 aligned positions) (Fig. 1). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no.a: 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 77.4 100 3 A. niger ATCC 1015 85.0 79.5 100 4 A. terreus ATCC 1012 84.0 78.0 87.8 100 5 A. flavipes ATCC 24487 79.5 75.3 82.3 86.8 100 6 A. candidus NRRL 303 84.0 82.3 82.4 79.8 78.7 100 7 A. restrictus NRRL 148 83.1 75.0 83.0 80.4 77.4 78.9 100 8 A. chevalieri ATCC 16443 63.8 60.0 62.6 60.4 57.8 61.5 71.6 100 9 A. granulosus NRRL 1932 58.9 61.2 58.2 58.5 60.6 60.1 57.4 63.8 100 10 A. ustus NRRL 275 59.0 60.4 59.2 60.5 58.7 58.7 59.2 60.1 92.5 100 11 A. sydowii NRRL 254 62.6 61.8 60.7 59.5 59.5 63.2 61.7 68.5 73.9 72.6 100 12 A. versicolor NRRL 238 62.6 61.3 62.3 60.5 60.1 62.3 61.7 69.8 73.3 70.7 98.1 100 13 A. nidulans ATCC 10074 63.7 60.4 61.3 60.0 60.4 62.1 61.1 67.5 74.8 75.0 94.2 94.8 100 a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table 1) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains (A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data (15). ..

    Article Title: Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
    Article Snippet: Species and strain b % Sequence identity with species no.a: 1 2 3 4 5 6 7 8 9 10 11 12 13 1 A. fumigatus ATCC 1022 100 2 A. flavus ATCC 11497 94.3 100 3 A. niger ATCC 1015 94.6 96.7 100 4 A. terreus ATCC 1012 95.6 95.2 95.8 100 5 A. flavipes ATCC 24487 95.9 96.5 95.8 96.5 100 6 A. candidus NRRL 303 95.6 96.9 96.1 95.6 96.7 100 7 A. restrictus NRRL 148 93.4 92.8 93.4 93.5 93.4 93.2 100 8 A. chevalieri ATCC 16443 94.5 94.5 94.5 94.6 95.0 94.6 95.8 100 9 A. granulosus NRRL 1932 93.9 94.5 95.2 93.4 95.0 93.9 93.0 94.3 100 10 A. ustus NRRL 275 93.5 94.1 94.8 93.0 94.6 93.5 92.6 93.9 99.6 100 11 A. sydowii NRRL 254 93.2 93.5 94.5 93.0 94.1 93.2 91.9 93.4 98.3 98.0 100 12 A. versicolor NRRL 238 93.4 93.7 94.6 92.8 94.3 93.4 92.1 93.5 98.5 98.5 99.1 100 13 A. nidulans ATCC 10074 94.5 94.7 95.4 93.6 94.8 94.1 92.8 93.4 98.7 98.7 97.4 97.6 100 a Values were calculated from pairwise alignments using sequence data determined in this study (for GenBank accession numbers, see Table 1) and the STRETCHER algorithm. .. Species numbers were assigned arbitrarily. b Sequences were derived from type strains or from non-type strains (A. flavus ATCC 11497, A. niger ATCC 1015, and A. restrictus NRRL 148) that exhibited perfect sequence identity with corresponding type strain reference data (35). on N ovem ber 5, 2012 by U N IV O F S U S S E X http://jcm .asm .org/ D ow nloaded from (Table 3). ..

    Article Title: Genome of Bacillus velezensis HT_B8 unravels endophytic strategies and biocontrol potential for grapevine sustainability
    Article Snippet: Grapevine trunk diseases (GTDs), including Botryosphaeria dieback, severely reduce grapevine yield and vineyard longevity worldwide.. Bacillus velezensis HT_B8, an endophytic bacterium isolated from Vitis vinifera cv.. Muscat of Italy in Bouargoub (Nabeul, Tunisia), efficiently colonizes grapevine tissues and strongly inhibits major GTD pathogens.



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    Biosynthetic pathways and genomic context of genes involved in the synthesis of fucose and rhamnose in <t>Synechocystis</t> sp. PCC 6803. (A) Schematic representation of the putative biosynthetic pathways of guanosine diphosphate-L-fucose (GDP-Fuc) and deoxythymidine diphosphate-L-rhamnose (dTDP-Rha). The red line indicates feed-back inhibition. (B) Locus of the genes encoding enzymes putatively involved in the biosynthetic pathways of GDP-Fuc and dTDP-Rha, with the genes knockout in this study highlighted in red, blue and green. Both the locus tag and gene name/symbol(s) are provided when available. Unk./Hyp. - genes encoding unknown or hypothetical proteins.
    Atcc Vr, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    atcc vr - by Bioz Stars, 2026-09
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    non encapsulated atcc 33277tm wild type strain  (ATCC)
    99
    ATCC non encapsulated atcc 33277tm wild type strain
    Biosynthetic pathways and genomic context of genes involved in the synthesis of fucose and rhamnose in <t>Synechocystis</t> sp. PCC 6803. (A) Schematic representation of the putative biosynthetic pathways of guanosine diphosphate-L-fucose (GDP-Fuc) and deoxythymidine diphosphate-L-rhamnose (dTDP-Rha). The red line indicates feed-back inhibition. (B) Locus of the genes encoding enzymes putatively involved in the biosynthetic pathways of GDP-Fuc and dTDP-Rha, with the genes knockout in this study highlighted in red, blue and green. Both the locus tag and gene name/symbol(s) are provided when available. Unk./Hyp. - genes encoding unknown or hypothetical proteins.
    Non Encapsulated Atcc 33277tm Wild Type Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+type+strains/Porphyromonas+gingivalis+(Coykendall+et+al%2E)+Shah+and+Collins/pm38674095-178-13-14
    Average 99 stars, based on 1 article reviews
    non encapsulated atcc 33277tm wild type strain - by Bioz Stars, 2026-09
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    Image Search Results


    Biosynthetic pathways and genomic context of genes involved in the synthesis of fucose and rhamnose in Synechocystis sp. PCC 6803. (A) Schematic representation of the putative biosynthetic pathways of guanosine diphosphate-L-fucose (GDP-Fuc) and deoxythymidine diphosphate-L-rhamnose (dTDP-Rha). The red line indicates feed-back inhibition. (B) Locus of the genes encoding enzymes putatively involved in the biosynthetic pathways of GDP-Fuc and dTDP-Rha, with the genes knockout in this study highlighted in red, blue and green. Both the locus tag and gene name/symbol(s) are provided when available. Unk./Hyp. - genes encoding unknown or hypothetical proteins.

    Journal: bioRxiv

    Article Title: Rhamnose biosynthesis is not impaired by the deletion of putative rfbC genes , slr0985 and slr1933 , in Synechocystis sp. PCC 6803

    doi: 10.1101/2025.03.27.645739

    Figure Lengend Snippet: Biosynthetic pathways and genomic context of genes involved in the synthesis of fucose and rhamnose in Synechocystis sp. PCC 6803. (A) Schematic representation of the putative biosynthetic pathways of guanosine diphosphate-L-fucose (GDP-Fuc) and deoxythymidine diphosphate-L-rhamnose (dTDP-Rha). The red line indicates feed-back inhibition. (B) Locus of the genes encoding enzymes putatively involved in the biosynthetic pathways of GDP-Fuc and dTDP-Rha, with the genes knockout in this study highlighted in red, blue and green. Both the locus tag and gene name/symbol(s) are provided when available. Unk./Hyp. - genes encoding unknown or hypothetical proteins.

    Article Snippet: The cyanobacterium Synechocystis sp. PCC 6803 wild type (sub-strain GT-Kazusa; glucose tolerant, with S-layer and non-motile) and strains were cultivated in BG11 medium ( ) at 30 °C under a 12 h light (25 μmol photons m −2 s −1 )/12 h dark regimen with orbital shaking (150 rpm).

    Techniques: Inhibition, Knock-Out

    Aggregation/sedimentation and cell wall ultrastructure of Synechocystis sp. PCC 6803 wild type (WT), and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains. (A) Micrographs of the strains at low cell densities highlighting the clumping phenotype of Δ fucS , scale bar: 20 μm. Inserts highlight differences in sedimentation. (B) Sedimentation index (%) at 0, 24 and 48 h. Data represent means ± SD ( n = 3). (C) Ultrastructure of the cell wall with the S-layer highlighted (arrow) and insert with the putative detached S-layer in Δ fucS also highlighted (asterisk). Scale bars: 200 nm. Cells were grown in BG11 medium at 30 °C under a 12 h light (25 μE m −2 s −1 )/12 h dark regimen, with orbital shaking at 150 rpm.

    Journal: bioRxiv

    Article Title: Rhamnose biosynthesis is not impaired by the deletion of putative rfbC genes , slr0985 and slr1933 , in Synechocystis sp. PCC 6803

    doi: 10.1101/2025.03.27.645739

    Figure Lengend Snippet: Aggregation/sedimentation and cell wall ultrastructure of Synechocystis sp. PCC 6803 wild type (WT), and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains. (A) Micrographs of the strains at low cell densities highlighting the clumping phenotype of Δ fucS , scale bar: 20 μm. Inserts highlight differences in sedimentation. (B) Sedimentation index (%) at 0, 24 and 48 h. Data represent means ± SD ( n = 3). (C) Ultrastructure of the cell wall with the S-layer highlighted (arrow) and insert with the putative detached S-layer in Δ fucS also highlighted (asterisk). Scale bars: 200 nm. Cells were grown in BG11 medium at 30 °C under a 12 h light (25 μE m −2 s −1 )/12 h dark regimen, with orbital shaking at 150 rpm.

    Article Snippet: The cyanobacterium Synechocystis sp. PCC 6803 wild type (sub-strain GT-Kazusa; glucose tolerant, with S-layer and non-motile) and strains were cultivated in BG11 medium ( ) at 30 °C under a 12 h light (25 μmol photons m −2 s −1 )/12 h dark regimen with orbital shaking (150 rpm).

    Techniques: Sedimentation

    Growth, total carbohydrates, released polysaccharides and capsular polysaccharides of Synechocystis sp. PCC 6803 wild type (WT), and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains. (A) Growth (optical density at 730 nm) and μg of chlorophyll a per mL of culture, (B) production of total carbohydrates (C), released polysaccharides, and (D) capsular polysaccharides expressed as μg of carbohydrates per μg of chlorophyll a . Data represent means ± SD ( n ≥ 3) and individual measurements are shown. Statistical analysis performed using one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparisons, is shown for the last time point. Significant differences are identified: *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.001), ****( p ≤ 0.0001).

    Journal: bioRxiv

    Article Title: Rhamnose biosynthesis is not impaired by the deletion of putative rfbC genes , slr0985 and slr1933 , in Synechocystis sp. PCC 6803

    doi: 10.1101/2025.03.27.645739

    Figure Lengend Snippet: Growth, total carbohydrates, released polysaccharides and capsular polysaccharides of Synechocystis sp. PCC 6803 wild type (WT), and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains. (A) Growth (optical density at 730 nm) and μg of chlorophyll a per mL of culture, (B) production of total carbohydrates (C), released polysaccharides, and (D) capsular polysaccharides expressed as μg of carbohydrates per μg of chlorophyll a . Data represent means ± SD ( n ≥ 3) and individual measurements are shown. Statistical analysis performed using one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparisons, is shown for the last time point. Significant differences are identified: *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.001), ****( p ≤ 0.0001).

    Article Snippet: The cyanobacterium Synechocystis sp. PCC 6803 wild type (sub-strain GT-Kazusa; glucose tolerant, with S-layer and non-motile) and strains were cultivated in BG11 medium ( ) at 30 °C under a 12 h light (25 μmol photons m −2 s −1 )/12 h dark regimen with orbital shaking (150 rpm).

    Techniques:

    Journal: bioRxiv

    Article Title: Rhamnose biosynthesis is not impaired by the deletion of putative rfbC genes , slr0985 and slr1933 , in Synechocystis sp. PCC 6803

    doi: 10.1101/2025.03.27.645739

    Figure Lengend Snippet:

    Article Snippet: The cyanobacterium Synechocystis sp. PCC 6803 wild type (sub-strain GT-Kazusa; glucose tolerant, with S-layer and non-motile) and strains were cultivated in BG11 medium ( ) at 30 °C under a 12 h light (25 μmol photons m −2 s −1 )/12 h dark regimen with orbital shaking (150 rpm).

    Techniques:

    Detection of rhamnose on the cells surface of Synechocystis sp. PCC 6803 wild type (WT), and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains using a rhamnose binding protein tagged with GFP (gp17-GFP). (A) Micrographs depicting the red autofluorescence signal and the green signal from the gp17-GFP. The right panels show the merging of the other two micrographs. Scale bar: 10 μm. (B) Quantification of fluorescence associated with gp17-GFP bound to rhamnose at the surface of the cells. Data represents means ± SD ( n = 3) and individual measurements are shown. Statistical analysis consists of one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparisons. Significant differences are identified: **( p ≤ 0.01) and ****( p ≤ 0.0001).

    Journal: bioRxiv

    Article Title: Rhamnose biosynthesis is not impaired by the deletion of putative rfbC genes , slr0985 and slr1933 , in Synechocystis sp. PCC 6803

    doi: 10.1101/2025.03.27.645739

    Figure Lengend Snippet: Detection of rhamnose on the cells surface of Synechocystis sp. PCC 6803 wild type (WT), and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains using a rhamnose binding protein tagged with GFP (gp17-GFP). (A) Micrographs depicting the red autofluorescence signal and the green signal from the gp17-GFP. The right panels show the merging of the other two micrographs. Scale bar: 10 μm. (B) Quantification of fluorescence associated with gp17-GFP bound to rhamnose at the surface of the cells. Data represents means ± SD ( n = 3) and individual measurements are shown. Statistical analysis consists of one-way analysis of variance (ANOVA), followed by Dunnett’s multiple comparisons. Significant differences are identified: **( p ≤ 0.01) and ****( p ≤ 0.0001).

    Article Snippet: The cyanobacterium Synechocystis sp. PCC 6803 wild type (sub-strain GT-Kazusa; glucose tolerant, with S-layer and non-motile) and strains were cultivated in BG11 medium ( ) at 30 °C under a 12 h light (25 μmol photons m −2 s −1 )/12 h dark regimen with orbital shaking (150 rpm).

    Techniques: Binding Assay, Fluorescence

    Analysis of the relative normalised expression of genes putatively related to the biosynthesis of deoxyhexoses and EPS in Synechocystis sp. PCC 6803 wild type (WT) and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains. RNA was extracted from cells of the different strains collected at three time points: 0, 7 and 21 days. RT-qPCR analysis of (A) gmd ( slr1072 ), rfbC1 ( slr0985 ) and rfbC2 ( slr1933 ) expression in Synechocystis sp. PCC 6803 wild type and Δ fucS ; (B) rfbB ( slr0982 ), rfbF ( slr0983 ), slr1610 (putative methyltransferase), gmd ( slr1072 ), fucS ( sll1213 ) and slr1933 ( rfbC2 ) expression in wild type and Δ rfbC1 ; and (C) rfbB ( slr0982 ), rfbF ( slr0983 ), slr1610 (putative methyltransferase), gmd ( slr1072 ) and fucS ( sll1213 ) expression in wild type and Δ rfbC1 Δ rfbC2 . The normalised fold expression of the target genes relative to wild type is represented at each time point. Data from three biological and three technical replicates were normalised against two reference genes ( sll1212 and sll1395 ), and error bars represent the standard deviations. Statistical analysis was performed using t -test, and significant differences are identified: *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.001), ****( p ≤ 0.0001).

    Journal: bioRxiv

    Article Title: Rhamnose biosynthesis is not impaired by the deletion of putative rfbC genes , slr0985 and slr1933 , in Synechocystis sp. PCC 6803

    doi: 10.1101/2025.03.27.645739

    Figure Lengend Snippet: Analysis of the relative normalised expression of genes putatively related to the biosynthesis of deoxyhexoses and EPS in Synechocystis sp. PCC 6803 wild type (WT) and Δ fucS , Δ rfbC1 and Δ rfbC1 Δ rfbC2 strains. RNA was extracted from cells of the different strains collected at three time points: 0, 7 and 21 days. RT-qPCR analysis of (A) gmd ( slr1072 ), rfbC1 ( slr0985 ) and rfbC2 ( slr1933 ) expression in Synechocystis sp. PCC 6803 wild type and Δ fucS ; (B) rfbB ( slr0982 ), rfbF ( slr0983 ), slr1610 (putative methyltransferase), gmd ( slr1072 ), fucS ( sll1213 ) and slr1933 ( rfbC2 ) expression in wild type and Δ rfbC1 ; and (C) rfbB ( slr0982 ), rfbF ( slr0983 ), slr1610 (putative methyltransferase), gmd ( slr1072 ) and fucS ( sll1213 ) expression in wild type and Δ rfbC1 Δ rfbC2 . The normalised fold expression of the target genes relative to wild type is represented at each time point. Data from three biological and three technical replicates were normalised against two reference genes ( sll1212 and sll1395 ), and error bars represent the standard deviations. Statistical analysis was performed using t -test, and significant differences are identified: *( p ≤ 0.05), **( p ≤ 0.01), ***( p ≤ 0.001), ****( p ≤ 0.0001).

    Article Snippet: The cyanobacterium Synechocystis sp. PCC 6803 wild type (sub-strain GT-Kazusa; glucose tolerant, with S-layer and non-motile) and strains were cultivated in BG11 medium ( ) at 30 °C under a 12 h light (25 μmol photons m −2 s −1 )/12 h dark regimen with orbital shaking (150 rpm).

    Techniques: Expressing, Quantitative RT-PCR