selective nmda receptor antagonist  (Tocris)

Journal: Methods and Protocols

Article Title: Comparison of the Utility of Amplitude–Spectral and Coherence Features of Psychotropic Drugs’ Action on ECoG Signal for Pharmaco-EEG Based Drug Screening in Rats

doi: 10.3390/mps9050123

Figure Lengend Snippet: Heat maps of median changes in coherence for δ, θ, α, and β frequency bands across lead pairs after administration of saline (NaCl), caffeine (CAF), sulpiride (SLP), haloperidol (HAL), dizocilpine (DIZ), galantamine (GAL), tropicamide (TRO), hydroxyzine (HXZ), aminophenylbutyric acid (PHB), phenazepam (PHE), atipamezole (ATI), dexmedetomidine (DEX), and 5-ethyl-5-phenyl-2,4,6(1H,3H,5H)-pyrimidinetrione (PhBT). Initial data were differences between mean frequency band coherence ~20 min after administration and values before administration (~30 min of baseline recording). For each group, 6–10 recordings were made.

Article Snippet: The training set, against which test-set pharmacological effects were classified, comprised previously published matrices of drug effects [ , , , , ]: the NMDA antagonist dizocilpine (Targetmol Chemicals Inc., Wellesley Hills, MA, USA); D2/D3 dopamine antagonists haloperidol (OOO Velpharm, Russia) and sulpiride (AO Organica, Novokuznetsk, Russia); the M-anticholinergic tropicamide (Tocris Bioscience, Bristol, UK); the H1/5-HT2A receptor blocker hydroxyzine (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany); the acetylcholinesterase inhibitor galantamine (Sopharma AD, Sofia, Bulgaria); the α2-adrenergic agonist dexmedetomidine (Orion Corporation, Espoo, Finland); and GABA-mimetics aminophenylbutyric acid (phenibut) (Tocris Bioscience, Bristol, UK) and bromdihydrochlorophenylbenzodiazepine (phenazepam) (AO Novosibkhimpharm, Novosibirsk, Russia) ( C).

Techniques: Saline

Journal: bioRxiv

Article Title: Compound- and sex-specific medial prefrontal cortex rewiring after prenatal THC and CBD exposure

doi: 10.64898/2026.02.13.705680

Figure Lengend Snippet: (A, B) Averaged fEPSP amplitudes plotted as a function of stimulus intensity. Synaptic strength remains stable across treatments, indicating that baseline recruitment does not account for the observed plasticity differences. (C) CBD-exposed males show a significant increase in the AMPA/NMDA ratio in layer V pyramidal neurons of the mPFC. (D) Representative traces of NMDA-EPSCs (red) evoked at +30 mV in the presence of 50 µM NBQX, and AMPA-EPSCs (blue) obtained by digital subtraction of the NMDA component from the dual-component current at +30 mV. (E, F) CBD alters the kinetics of evoked NMDA currents. Male CBD-exposed offspring exhibit slower rise times, whereas CBD-exposed females display faster decay kinetics. (A, B) Each point represents the group mean at the corresponding stimulus intensity; data are shown as mean ± SEM in XY plots and analyzed using a multiple-measures Mann–Whitney test. (C, E, F) Each point represents a single neuron; data are presented as violin plots (median and 25th–75th percentiles) and analyzed using two-way ANOVA followed by Šídák’s multiple-comparison test. Only statistically significant differences (p < 0.05) are indicated.

Article Snippet: GABA-sIPSCs were recorded at − 70 mV in presence of 20 μM CNQX (6-Cyano-7-nitroquinoxaline-2,3-dione disodium, an AMPA receptor antagonist, Tocris) and L-APV 50 μM (DL-2-Amino-5-phosphonopentanoic acid, a selective NMDA receptor antagonist, Tocris).

Techniques: MANN-WHITNEY, Comparison