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    Bio-Rad nitrocellulose membrane sheets
    Nitrocellulose Membrane Sheets, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 32538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nitrocellulose+membrane+sheets/Nitrocellulose+Membrane/us12544413-175-7-10
    Average 99 stars, based on 32538 article reviews
    nitrocellulose membrane sheets - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Efficacy of an Innovative Poly-Component Formulation in Counteracting Human Dermal Fibroblast Aging by Influencing Oxidative and Inflammatory Pathways
    Article Snippet: Then, the supernatant was collected after centrifugation at 17,949× g , eliminating cell debris, and the total protein content was assessed using DC Protein Assay (BioRad, Hercules, CA, USA). .. Equal amounts of protein solutions (25 μg/well) were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 °C. .. After blocking of the specific sites with 5% nonfat dry milk, the membranes were incubated overnight at 4 °C with mouse monoclonal antibody anti-p21 1:1000 (Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal antibody anti-COL1A1 1:1000 (Boster Biological Technology, Pleasanton, CA, USA), rabbit monoclonal antibody anti-phospho-Nrf2 (phospho-S40) 1:2000 (Abcam, Cambridge, UK), rabbit monoclonal antibody anti-phospho-NF-κB (phospho-S536) 1:1000 (Cell Signaling Technology), rabbit monoclonal antibody anti-NF-κB 1:1000 (Cell Signaling Technology), and mouse monoclonal antibody anti-GAPDH 1:2000 (Immunological Sciences, Rome, Italy).

    Article Title: Efficacy of an Innovative Poly-Component Formulation in Counteracting Human Dermal Fibroblast Aging by Influencing Oxidative and Inflammatory Pathways
    Article Snippet: Then, the supernatant was collected after centrifugation at 17,949× g, eliminating cell debris, and the total protein content was assessed using DC Protein Assay (BioRad, Hercules, CA, USA). .. Equal amounts of protein solutions (25 μg/well) were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 ◦C. .. After blocking of the specific sites with 5% nonfat dry milk, the membranes were incubated overnight at 4 ◦C with mouse monoclonal antibody anti-p21 1:1000 (Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal antibody anti-COL1A1 1:1000 (Boster Biological Technology, Pleasanton, CA, USA), rabbit monoclonal antibody anti-phospho-Nrf2 (phospho-S40) 1:2000 (Abcam, Cambridge, UK), rabbit monoclonal antibody anti-phospho-NF-κB (phospho-S536) 1:1000 (Cell Signaling Technology), rabbit monoclonal antibody anti-NF-κB 1:1000 (Cell Signaling Technology), and mouse monoclonal antibody anti-GAPDH 1:2000 (Immunological Sciences, Rome, Italy).

    Article Title: Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts
    Article Snippet: Then, to eliminate cell debris, the samples were centrifuged at 17,949× g , and the total protein concentration was determined by DC Protein Assay (BioRad, Hercules, CA, USA). .. A total of 25 μg of proteins was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 °C. ..

    Article Title: Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts.
    Article Snippet: Then, to eliminate cell debris, the samples were centrifuged at 17,949× g, and the total protein concentration was determined by DC Protein Assay (BioRad, Hercules, CA, USA). .. A total of 25 μg of proteins was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 ◦C. ..

    Membrane:

    Article Title: Efficacy of an Innovative Poly-Component Formulation in Counteracting Human Dermal Fibroblast Aging by Influencing Oxidative and Inflammatory Pathways
    Article Snippet: Then, the supernatant was collected after centrifugation at 17,949× g , eliminating cell debris, and the total protein content was assessed using DC Protein Assay (BioRad, Hercules, CA, USA). .. Equal amounts of protein solutions (25 μg/well) were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 °C. .. After blocking of the specific sites with 5% nonfat dry milk, the membranes were incubated overnight at 4 °C with mouse monoclonal antibody anti-p21 1:1000 (Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal antibody anti-COL1A1 1:1000 (Boster Biological Technology, Pleasanton, CA, USA), rabbit monoclonal antibody anti-phospho-Nrf2 (phospho-S40) 1:2000 (Abcam, Cambridge, UK), rabbit monoclonal antibody anti-phospho-NF-κB (phospho-S536) 1:1000 (Cell Signaling Technology), rabbit monoclonal antibody anti-NF-κB 1:1000 (Cell Signaling Technology), and mouse monoclonal antibody anti-GAPDH 1:2000 (Immunological Sciences, Rome, Italy).

    Article Title: Efficacy of an Innovative Poly-Component Formulation in Counteracting Human Dermal Fibroblast Aging by Influencing Oxidative and Inflammatory Pathways
    Article Snippet: Then, the supernatant was collected after centrifugation at 17,949× g, eliminating cell debris, and the total protein content was assessed using DC Protein Assay (BioRad, Hercules, CA, USA). .. Equal amounts of protein solutions (25 μg/well) were separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 ◦C. .. After blocking of the specific sites with 5% nonfat dry milk, the membranes were incubated overnight at 4 ◦C with mouse monoclonal antibody anti-p21 1:1000 (Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal antibody anti-COL1A1 1:1000 (Boster Biological Technology, Pleasanton, CA, USA), rabbit monoclonal antibody anti-phospho-Nrf2 (phospho-S40) 1:2000 (Abcam, Cambridge, UK), rabbit monoclonal antibody anti-phospho-NF-κB (phospho-S536) 1:1000 (Cell Signaling Technology), rabbit monoclonal antibody anti-NF-κB 1:1000 (Cell Signaling Technology), and mouse monoclonal antibody anti-GAPDH 1:2000 (Immunological Sciences, Rome, Italy).

    Article Title: Composition with antibacterial and re-epithelializing action including probiotics
    Article Snippet: .. The proteins were transferred on 0.45 μm nitrocellulose membrane sheets (BioRad, Hercules, CA) for 1 hour at 4° C. at 70 V using Mini Trans-Blot Cell (BioRad, Hercules, CA) equipment. .. The membranes were blocked with 5% of fatty acid-free milk for 1 hour at room temperature and then incubated overnight at 4° C. with 1:500 polyclonal rabbit anti-iNOS antibody (Cell Signaling Technology, CA) or 1:1000 anti-β-actin antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

    Article Title: Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts
    Article Snippet: Then, to eliminate cell debris, the samples were centrifuged at 17,949× g , and the total protein concentration was determined by DC Protein Assay (BioRad, Hercules, CA, USA). .. A total of 25 μg of proteins was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 °C. ..

    Article Title: Total protein concentration and stability of Amb a 1 in glycerinated ragweed sublingual immunotherapy stored at room temperature and refrigerated cold temperature.
    Article Snippet: Funding information Stallergenes Greer Abstract Background: Few studies have investigated optimal storage conditions or expiration dates for sublingual immunotherapy (SLIT) formulations prepared from glycerinated allergen extracts.. Hypothesis/Objectives: The objective of this study was to compare concentrations of short ragweed major allergen (Amb a 1) and total protein in SLIT formulations stored at two different temperatures.. It was hypothesised that protein concentrations would show greater decline over time in a formulation stored at room temperature (RT) than in one stored under refrigeration.

    Article Title: Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts.
    Article Snippet: Then, to eliminate cell debris, the samples were centrifuged at 17,949× g, and the total protein concentration was determined by DC Protein Assay (BioRad, Hercules, CA, USA). .. A total of 25 μg of proteins was separated by 10% SDS-polyacrylamide gel electrophoresis and transferred onto 0.45 μm nitrocellulose membrane sheets (BioRad) for 1 h at 4 ◦C. ..

    Article Title: Up-Regulation of Cyclooxygenase-2 (COX-2) Expression by Temozolomide (TMZ) in Human Glioblastoma (GBM) Cell Lines.
    Article Snippet: .. Proteins were transferred onto 0.45 μm nitrocellulose membrane sheets (Bio-Rad) for 1 h at 4 ◦C at 70 V using a Mini Trans-Blot Cell apparatus (Bio-Rad). .. Non-specific binding sites were blocked with 5% non-fat dry milk for 1 h at room temperature and then incubated overnight at 4 ◦C with primary antibodies: rabbit monoclonal anti-COX-2 (Cell Signaling Technology, Danvers, MA, USA; dilution 1:1000), mouse monoclonal antibody anti-MGMT (BD Biosciences, San José, CA, USA; dilution 1:500), mouse monoclonal antibody anti-SOX2 (Origene, 9620 Medical Center Drive Suite 200 Rockville, MD, USA; dilution 1:1000), rabbit polyclonal antibody anti-β-catenin (Cell Signaling Technology, Danvers, MA, USA; dilution 1:1000), and mouse monoclonal antibody for anti-β-actin antibody (Bio-Rad, Hercules, CA, USA; dilution 1:1000).

    Article Title: Primary digestive cathepsins L of Tribolium castaneum larvae: Proteomic identification, properties, comparison with human lysosomal cathepsin L.
    Article Snippet: Sephadex G-75 was from GE Healthcare (Chicago, IL, USA). .. Nitrocellulose membrane sheets with a 0.45-mm pore size were from Bio-Rad (Hercules, CA, USA). .. Сentrifugal filters Amicon Ultra 3K were from Millipore-Sigma (Burlington, MA, USA).

    Electrophoresis:

    Article Title: Total protein concentration and stability of Amb a 1 in glycerinated ragweed sublingual immunotherapy stored at room temperature and refrigerated cold temperature.
    Article Snippet: Funding information Stallergenes Greer Abstract Background: Few studies have investigated optimal storage conditions or expiration dates for sublingual immunotherapy (SLIT) formulations prepared from glycerinated allergen extracts.. Hypothesis/Objectives: The objective of this study was to compare concentrations of short ragweed major allergen (Amb a 1) and total protein in SLIT formulations stored at two different temperatures.. It was hypothesised that protein concentrations would show greater decline over time in a formulation stored at room temperature (RT) than in one stored under refrigeration.

    Diffusion-based Assay:

    Article Title: Total protein concentration and stability of Amb a 1 in glycerinated ragweed sublingual immunotherapy stored at room temperature and refrigerated cold temperature.
    Article Snippet: Funding information Stallergenes Greer Abstract Background: Few studies have investigated optimal storage conditions or expiration dates for sublingual immunotherapy (SLIT) formulations prepared from glycerinated allergen extracts.. Hypothesis/Objectives: The objective of this study was to compare concentrations of short ragweed major allergen (Amb a 1) and total protein in SLIT formulations stored at two different temperatures.. It was hypothesised that protein concentrations would show greater decline over time in a formulation stored at room temperature (RT) than in one stored under refrigeration.

    Pore Size:

    Article Title: Primary digestive cathepsins L of Tribolium castaneum larvae: Proteomic identification, properties, comparison with human lysosomal cathepsin L.
    Article Snippet: Sephadex G-75 was from GE Healthcare (Chicago, IL, USA). .. Nitrocellulose membrane sheets with a 0.45-mm pore size were from Bio-Rad (Hercules, CA, USA). .. Сentrifugal filters Amicon Ultra 3K were from Millipore-Sigma (Burlington, MA, USA).



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    Effect of central leptin on PPARβ/δ protein levels in the heart. Western-Blot analysis of PPARβ/δ in 50 µg of total extracts from cardiac ventricles after central saline or leptin infusion. The values are expressed relative to the SS group. Data are the mean ± SEM (n = 5) per group of animals. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. Different letters indicate significant differences ( p ≤ 0.05). Groups: SS: saline-infused rats fed ad libitum ; PF: saline-infused pair-fed rats; Lep: leptin-infused rats; Lep+GSK0660: leptin-infused rats and treated with GSK0660. A representative Red Ponceau staining of the <t>nitrocellulose</t> membrane for total protein normalization prior to immunodetection and the quantitative densitometry readings of PPARβ/δ protein from the Western-Blot analysis are depicted in .
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    Effect of central leptin on PPARβ/δ protein levels in the heart. Western-Blot analysis of PPARβ/δ in 50 µg of total extracts from cardiac ventricles after central saline or leptin infusion. The values are expressed relative to the SS group. Data are the mean ± SEM (n = 5) per group of animals. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. Different letters indicate significant differences ( p ≤ 0.05). Groups: SS: saline-infused rats fed ad libitum ; PF: saline-infused pair-fed rats; Lep: leptin-infused rats; Lep+GSK0660: leptin-infused rats and treated with GSK0660. A representative Red Ponceau staining of the nitrocellulose membrane for total protein normalization prior to immunodetection and the quantitative densitometry readings of PPARβ/δ protein from the Western-Blot analysis are depicted in .

    Journal: Biomolecules

    Article Title: Central Actions of Leptin Induce an Atrophic Pattern and Improves Heart Function in Lean Normoleptinemic Rats via PPARβ/δ Activation

    doi: 10.3390/biom14081028

    Figure Lengend Snippet: Effect of central leptin on PPARβ/δ protein levels in the heart. Western-Blot analysis of PPARβ/δ in 50 µg of total extracts from cardiac ventricles after central saline or leptin infusion. The values are expressed relative to the SS group. Data are the mean ± SEM (n = 5) per group of animals. Statistical analysis was performed using one-way ANOVA followed by Tukey’s test. Different letters indicate significant differences ( p ≤ 0.05). Groups: SS: saline-infused rats fed ad libitum ; PF: saline-infused pair-fed rats; Lep: leptin-infused rats; Lep+GSK0660: leptin-infused rats and treated with GSK0660. A representative Red Ponceau staining of the nitrocellulose membrane for total protein normalization prior to immunodetection and the quantitative densitometry readings of PPARβ/δ protein from the Western-Blot analysis are depicted in .

    Article Snippet: Samples were previously mixed with SDS sample buffer and boiled at 95 °C for 10 min. Proteins were transferred to nitrocellulose sheets (0.2 μm, Bio-Rad, Hercules, CA, USA) and incubated overnight (12–16 h) at 4 °C with the appropriate primary antibodies, followed by incubation at room temperature for 2 h with corresponding secondary antibody conjugated with horseradish peroxidase.

    Techniques: Western Blot, Saline, Staining, Membrane, Immunodetection