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nikon nis elements software  (Nikon)


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    Nikon nikon nis elements software
    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Nikon Nis Elements Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 39515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+elements+software/NIS-Elements/pmc12811640-111-7-7
    Average 99 stars, based on 39515 article reviews
    nikon nis elements software - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software

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    Article Snippet: Finally, slides were cleaned with 70% ethanol, dried, and examined under a light microscope (Nikon, Melville, NY). .. Mirroring the previously published methodologies [ , ], the downgrowth and GT area were measured using the Nikon Elements software (NIS; Nikon, Melville, NY) with the line measurement and area measurement tools, respectively. ..

    Article Title: tRNA synthetase activity is required for stress granule and P-body assembly.
    Article Snippet: For live-cell microscopy, media was exchanged to FluoroBrite (Gibco A1896701) supplemented with FBE and GlutaMAX 1 h before treatment. .. Imaging was performed using Nikon Elements software and the 40× objective of a Nikon Eclipse Ti2 microscope equipped with a Lumencor Spectra III light engine, Andor Life 888 EMCCD camera, and a Tokai Hit stage-top incubator system set to 37°C and 5% CO2. .. To assess whether stress granules formed over time upon halofuginone treatment, images were collected every 2 h for 16 h. To determine whether stress granules formed in halofuginonetreated cells in the presence or absence of arsenite or rocaglamide A, images were collected every 30 min for 3 h. The percentage of cells with either stress granules and/or P-bodies was quantified manually using the Cell Counter plugin in ImageJ (Schindelin et al. 2012).

    Article Title: A Novel Cell-Cell Communication Structure: Tanycyte and Cilia Hypothalamic Unifying Glia-cilia Structure (HUGS)
    Article Snippet: The secondary antibodies include donkey conjugated Alexa Fluor 488, and 647 (1:1000; Invitrogen, Carlsbad, CA, USA and Jackson Immuno Research, West Grove, PA, USA) against appropriate species according to the corresponding primary.  The slides were then washed in PBS and counterstained with Hoechst nuclear stain (1:1000; catalog no. H3570, Thermo Fisher Scientific) for 5 min at room temperature. .. Coverslips were mounted using SlowFade Diamond Antifade Mountant (catalog no. S36972 , Thermo Fisher Scientific). All primary and secondary solutions were made in the blocking solution described above.  Imaging and Analysis Images were captured using a Nikon Ax confocal microscope (40X water lens objective) and cilia HUGS were identified and analyzed using Nikon Elements Software (NIS Elements). ..

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    Article Title: tRNA synthetase activity is required for stress granule and P-body assembly
    Article Snippet: For live-cell microscopy, media was exchanged to FluoroBrite (Gibco A1896701) supplemented with FBE and GlutaMAX 1 h before treatment. .. Imaging was performed using Nikon Elements software and the 40× objective of a Nikon Eclipse Ti2 microscope equipped with a Lumencor Spectra III light engine, Andor Life 888 EMCCD camera, and a Tokai Hit stage-top incubator system set to 37°C and 5% CO 2 . .. To assess whether stress granules formed over time upon halofuginone treatment, images were collected every 2 h for 16 h. To determine whether stress granules formed in halofuginone-treated cells in the presence or absence of arsenite or rocaglamide A, images were collected every 30 min for 3 h. The percentage of cells with either stress granules and/or P-bodies was quantified manually using the Cell Counter plugin in ImageJ ( ).

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    Article Title: Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.
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    Imaging:

    Article Title: tRNA synthetase activity is required for stress granule and P-body assembly.
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    Article Title: A Novel Cell-Cell Communication Structure: Tanycyte and Cilia Hypothalamic Unifying Glia-cilia Structure (HUGS)
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    Article Title: tRNA synthetase activity is required for stress granule and P-body assembly
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    Microscopy:

    Article Title: tRNA synthetase activity is required for stress granule and P-body assembly.
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    Article Title: A Novel Cell-Cell Communication Structure: Tanycyte and Cilia Hypothalamic Unifying Glia-cilia Structure (HUGS)
    Article Snippet: The secondary antibodies include donkey conjugated Alexa Fluor 488, and 647 (1:1000; Invitrogen, Carlsbad, CA, USA and Jackson Immuno Research, West Grove, PA, USA) against appropriate species according to the corresponding primary.  The slides were then washed in PBS and counterstained with Hoechst nuclear stain (1:1000; catalog no. H3570, Thermo Fisher Scientific) for 5 min at room temperature. .. Coverslips were mounted using SlowFade Diamond Antifade Mountant (catalog no. S36972 , Thermo Fisher Scientific). All primary and secondary solutions were made in the blocking solution described above.  Imaging and Analysis Images were captured using a Nikon Ax confocal microscope (40X water lens objective) and cilia HUGS were identified and analyzed using Nikon Elements Software (NIS Elements). ..

    Article Title: Müller glial and microglial responses coupled to recovery of cone photoreceptors following limited cone ablation in zebrafish retina
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    Article Title: tRNA synthetase activity is required for stress granule and P-body assembly
    Article Snippet: For live-cell microscopy, media was exchanged to FluoroBrite (Gibco A1896701) supplemented with FBE and GlutaMAX 1 h before treatment. .. Imaging was performed using Nikon Elements software and the 40× objective of a Nikon Eclipse Ti2 microscope equipped with a Lumencor Spectra III light engine, Andor Life 888 EMCCD camera, and a Tokai Hit stage-top incubator system set to 37°C and 5% CO 2 . .. To assess whether stress granules formed over time upon halofuginone treatment, images were collected every 2 h for 16 h. To determine whether stress granules formed in halofuginone-treated cells in the presence or absence of arsenite or rocaglamide A, images were collected every 30 min for 3 h. The percentage of cells with either stress granules and/or P-bodies was quantified manually using the Cell Counter plugin in ImageJ ( ).

    Article Title: Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.
    Article Snippet: .. Zeiss Definite Focus system was used and cells were imaged for 4–8 h. For observing pyrenoid phase behaviours of WT and key1-1 mutant cells with RBCS1–Venus during cell division, cell samples were imaged at room temperature every 20 min for 2 h and then every 5 min for 6 h on a Nikon A1R scanning confocal microscope in resonant scanning mode using Nikon Elements software, a ×100 1.49 NA objective and pinhole size 49.81 μm. ..

    Article Title: Müller glial and microglial responses coupled to recovery of cone photoreceptors following limited cone ablation in zebrafish retina.
    Article Snippet: .. Confocal Microscopy, Image Viewing, and Image Analysis All imaging was performed on a Nikon Crest Optics X-Light spinning disc confocal microscope running Nikon Elements software, using 20X dry objective (Plan Apo λ 20X Air 0.75 NA DIC), in some cases using 1.5X magnification boost, and in some cases using image stitching to obtain images of entire retinal cryosections or images of whole flattened retinas. ..

    Blocking Assay:

    Article Title: A Novel Cell-Cell Communication Structure: Tanycyte and Cilia Hypothalamic Unifying Glia-cilia Structure (HUGS)
    Article Snippet: The secondary antibodies include donkey conjugated Alexa Fluor 488, and 647 (1:1000; Invitrogen, Carlsbad, CA, USA and Jackson Immuno Research, West Grove, PA, USA) against appropriate species according to the corresponding primary.  The slides were then washed in PBS and counterstained with Hoechst nuclear stain (1:1000; catalog no. H3570, Thermo Fisher Scientific) for 5 min at room temperature. .. Coverslips were mounted using SlowFade Diamond Antifade Mountant (catalog no. S36972 , Thermo Fisher Scientific). All primary and secondary solutions were made in the blocking solution described above.  Imaging and Analysis Images were captured using a Nikon Ax confocal microscope (40X water lens objective) and cilia HUGS were identified and analyzed using Nikon Elements Software (NIS Elements). ..

    Staining:

    Article Title: Obesity-induced pyroptotic adipocyte death leads to TREM2-dependent macrophage dysfunction and adipose tissue inflammation
    Article Snippet: IWAT and GWAT were fixed in 10% formalin (Sigma, HT50128), paraffin-embedded, and sectioned at 5 μm thickness. .. Sections were stained with hematoxylin (BBC Biochemical, MA010081 ) and eosin (BBC Biochemical, 3610) and visualized using Nikon Elements software (NIS BR 5.10.00). ..

    Mutagenesis:

    Article Title: Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.
    Article Snippet: .. Zeiss Definite Focus system was used and cells were imaged for 4–8 h. For observing pyrenoid phase behaviours of WT and key1-1 mutant cells with RBCS1–Venus during cell division, cell samples were imaged at room temperature every 20 min for 2 h and then every 5 min for 6 h on a Nikon A1R scanning confocal microscope in resonant scanning mode using Nikon Elements software, a ×100 1.49 NA objective and pinhole size 49.81 μm. ..

    Confocal Microscopy:

    Article Title: Müller glial and microglial responses coupled to recovery of cone photoreceptors following limited cone ablation in zebrafish retina.
    Article Snippet: .. Confocal Microscopy, Image Viewing, and Image Analysis All imaging was performed on a Nikon Crest Optics X-Light spinning disc confocal microscope running Nikon Elements software, using 20X dry objective (Plan Apo λ 20X Air 0.75 NA DIC), in some cases using 1.5X magnification boost, and in some cases using image stitching to obtain images of entire retinal cryosections or images of whole flattened retinas. ..



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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon <t>NIS</t> <t>Elements</t> <t>software.</t> All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: A. brasilense AR mutant exhibits elevated levels of intracellular c-di-GMP. Colony morphology of wild-type A. brasilense Sp7 ( A ) and the AR mutant ( B ) expressing a c-di-GMP biosensor (pFY4535). Cultures were grown for five days at 30 °C on Nfb∗ agar supplemented with KNO 3 . The intracellular c-di-GMP concentration is proportional to the expression of TurboRFP (red), resulting in a red colony color. The AR mutant's more intense coloration indicates higher c-di-GMP accumulation than in the WT. Representative fluorescence microscopy of individual WT and AR mutant cells. Images were captured on a Nikon Eclipse TE2000-U microscope using the following excitation/emission wavelengths: 489/519 nm for AmCyan (green) and 553/574 nm for TurboRFP (red). Gray images are intensity surface plots corresponding to the fluorescence emitted by TurboRFP. Images were processed using Nikon NIS Elements software. All images shown are representative of three independent experiments. Scale bars, 10 mm (colonies); 10 μm (individual cells). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Obtained images were processed and analyzed using Nikon NIS Elements software.

    Techniques: Mutagenesis, Expressing, Concentration Assay, Fluorescence, Microscopy, Software