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eclipse ni e upright fluorescence microscope  (Nikon)


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    Nikon eclipse ni e upright fluorescence microscope
    Eclipse Ni E Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ni-e+fluorescence+microscope/Objectives/pmc12863670-72-9-8
    Average 99 stars, based on 59633 article reviews
    eclipse ni e upright fluorescence microscope - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Protein corona as a mediator in antibiotic adsorption onto microplastics: Mechanisms and implications.
    Article Snippet: Microplastics are emerging pollutants capable of adsorbing antibiotics in the environment through interactions mediated by biological molecules such as proteins, ultimately posing risks to human health.. However, direct evidence demonstrating that microplastics and antibiotics form chemical-adsorption products has not been explored.. One key mechanism contributing to their co-exposure risks during their transmission is biofilm formation, particularly the development of a protein corona, which may also serve as a potential virulence mechanism.

    Article Title: Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.
    Article Snippet: Institute of Science Education and Research, Mohali, Punjab, India Correspondence Rajesh Ramachandran, Room 3F10, Academic Block-1, Indian Institute of Science Education and Research, Mohali, Knowledge city, Sector 81, SAS Nagar, Manauli PO, PIN-140306, Mohali, Punjab, India.. Email: rajeshra@iisermohali.ac.in Present address Simran Kaur, Applied Stem Cell, Milpitas, CA, USA.. Vijithkumar Vijayan, Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.

    Article Title: Mechanistic Insights into Dibasic Iminosugars as pH-Selective Pharmacological Chaperones to Stabilize Human α-Galactosidase
    Article Snippet: Images were acquired by using Nikon Ni-E fluorescence microscope and quantified by using ImageJ software.

    Article Title: Trichoderma species from rhizosphere of Oxalis corymbosa release volatile organic compounds inhibiting the seed germination and growth of Echinochloa colona
    Article Snippet: For microscopic morphology, photographs were taken with a Nikon Ni-E fluorescence microscope (Tokyo, Japan) connected to a DP controller digital camera.

    Article Title: A sustainable emulsion for separation and Raman identification of microplastics and nanoplastics
    Article Snippet: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become an issue of increasing concern worldwide.. The currently established approaches and tools for the elimination of the emerging contaminants, in particular microand nano-scale plastic particles, from environmental and food matrices have been often limited to the use of poorly sustainable materials and complicated preparation/operation.. Here we show a conceptually new strategy for the removal of microplastics and nanoplastics using an olive oil-based emulsion.

    Article Title: β-Cyclodextrin nanosponges for the ocular delivery of therapeutic Micro-RNA in a Mouse model of retinitis Pigmentosa: A proof of concept study.
    Article Snippet: The exploitation of micro-RNA (miR) sequences as therapeutics has become highly attractive for the treatment of several diseases, including those still lacking effective cures such as retinitis pigmentosa (RP).. Interestingly, miR155-5p plays a role in photo-oxidative inflammation in wild-type mice and is up-regulated in rd10 mice showing peak rod degeneration, suggesting its inhibition by the corresponding anti-miR as a viable therapeutic strategy for RP.. However, biomedical application of (anti-)miRs is limited by their oligonucleotide nature, suffering from low solubility and bioavailability along with a very low half-life in vivo due to enzymatic degradation.

    Article Title: Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
    Article Snippet: Slices were manually analyzed under a Nikon Ni-E fluorescence microscope equipped with Nikon DS-Ri2 camera and NIS-Elements C 4.20 (Nikon, Italy).

    Article Title: Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV).
    Article Snippet: The Stimulator of Interferon Genes (STING, also known as MITA/ERYS/MPYS) is an adaptor molecule that plays a crucial role in the RLR pathway and responds to DNA and RNA viruses.. In the present study, we have identified two novel isoforms of STING (the canonical form named as LcSTINGa and its alternative splicing isoform named as LcSTINGb) from teleost Lates calcarifer.. LcSTINGa has an ORF of 1230 bp, encoding a 409 amino acid protein, while its alternative splicing variant, LcSTINGb, features an ORF of 987 bp, encoding 328 amino acids.

    Microscopy:

    Article Title: Protein corona as a mediator in antibiotic adsorption onto microplastics: Mechanisms and implications.
    Article Snippet: Microplastics are emerging pollutants capable of adsorbing antibiotics in the environment through interactions mediated by biological molecules such as proteins, ultimately posing risks to human health.. However, direct evidence demonstrating that microplastics and antibiotics form chemical-adsorption products has not been explored.. One key mechanism contributing to their co-exposure risks during their transmission is biofilm formation, particularly the development of a protein corona, which may also serve as a potential virulence mechanism.

    Article Title: Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.
    Article Snippet: Institute of Science Education and Research, Mohali, Punjab, India Correspondence Rajesh Ramachandran, Room 3F10, Academic Block-1, Indian Institute of Science Education and Research, Mohali, Knowledge city, Sector 81, SAS Nagar, Manauli PO, PIN-140306, Mohali, Punjab, India.. Email: rajeshra@iisermohali.ac.in Present address Simran Kaur, Applied Stem Cell, Milpitas, CA, USA.. Vijithkumar Vijayan, Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.

    Article Title: Mechanistic Insights into Dibasic Iminosugars as pH-Selective Pharmacological Chaperones to Stabilize Human α-Galactosidase
    Article Snippet: Images were acquired by using Nikon Ni-E fluorescence microscope and quantified by using ImageJ software.

    Article Title: Trichoderma species from rhizosphere of Oxalis corymbosa release volatile organic compounds inhibiting the seed germination and growth of Echinochloa colona
    Article Snippet: For microscopic morphology, photographs were taken with a Nikon Ni-E fluorescence microscope (Tokyo, Japan) connected to a DP controller digital camera.

    Article Title: A sustainable emulsion for separation and Raman identification of microplastics and nanoplastics
    Article Snippet: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become an issue of increasing concern worldwide.. The currently established approaches and tools for the elimination of the emerging contaminants, in particular microand nano-scale plastic particles, from environmental and food matrices have been often limited to the use of poorly sustainable materials and complicated preparation/operation.. Here we show a conceptually new strategy for the removal of microplastics and nanoplastics using an olive oil-based emulsion.

    Article Title: β-Cyclodextrin nanosponges for the ocular delivery of therapeutic Micro-RNA in a Mouse model of retinitis Pigmentosa: A proof of concept study.
    Article Snippet: The exploitation of micro-RNA (miR) sequences as therapeutics has become highly attractive for the treatment of several diseases, including those still lacking effective cures such as retinitis pigmentosa (RP).. Interestingly, miR155-5p plays a role in photo-oxidative inflammation in wild-type mice and is up-regulated in rd10 mice showing peak rod degeneration, suggesting its inhibition by the corresponding anti-miR as a viable therapeutic strategy for RP.. However, biomedical application of (anti-)miRs is limited by their oligonucleotide nature, suffering from low solubility and bioavailability along with a very low half-life in vivo due to enzymatic degradation.

    Article Title: Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
    Article Snippet: Slices were manually analyzed under a Nikon Ni-E fluorescence microscope equipped with Nikon DS-Ri2 camera and NIS-Elements C 4.20 (Nikon, Italy).

    Article Title: Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV).
    Article Snippet: The Stimulator of Interferon Genes (STING, also known as MITA/ERYS/MPYS) is an adaptor molecule that plays a crucial role in the RLR pathway and responds to DNA and RNA viruses.. In the present study, we have identified two novel isoforms of STING (the canonical form named as LcSTINGa and its alternative splicing isoform named as LcSTINGb) from teleost Lates calcarifer.. LcSTINGa has an ORF of 1230 bp, encoding a 409 amino acid protein, while its alternative splicing variant, LcSTINGb, features an ORF of 987 bp, encoding 328 amino acids.

    Software:

    Article Title: Protein corona as a mediator in antibiotic adsorption onto microplastics: Mechanisms and implications.
    Article Snippet: Microplastics are emerging pollutants capable of adsorbing antibiotics in the environment through interactions mediated by biological molecules such as proteins, ultimately posing risks to human health.. However, direct evidence demonstrating that microplastics and antibiotics form chemical-adsorption products has not been explored.. One key mechanism contributing to their co-exposure risks during their transmission is biofilm formation, particularly the development of a protein corona, which may also serve as a potential virulence mechanism.

    Article Title: Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.
    Article Snippet: Institute of Science Education and Research, Mohali, Punjab, India Correspondence Rajesh Ramachandran, Room 3F10, Academic Block-1, Indian Institute of Science Education and Research, Mohali, Knowledge city, Sector 81, SAS Nagar, Manauli PO, PIN-140306, Mohali, Punjab, India.. Email: rajeshra@iisermohali.ac.in Present address Simran Kaur, Applied Stem Cell, Milpitas, CA, USA.. Vijithkumar Vijayan, Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.

    Article Title: Mechanistic Insights into Dibasic Iminosugars as pH-Selective Pharmacological Chaperones to Stabilize Human α-Galactosidase
    Article Snippet: Images were acquired by using Nikon Ni-E fluorescence microscope and quantified by using ImageJ software.

    Article Title: Trichoderma species from rhizosphere of Oxalis corymbosa release volatile organic compounds inhibiting the seed germination and growth of Echinochloa colona
    Article Snippet: For microscopic morphology, photographs were taken with a Nikon Ni-E fluorescence microscope (Tokyo, Japan) connected to a DP controller digital camera.

    Article Title: A sustainable emulsion for separation and Raman identification of microplastics and nanoplastics
    Article Snippet: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become an issue of increasing concern worldwide.. The currently established approaches and tools for the elimination of the emerging contaminants, in particular microand nano-scale plastic particles, from environmental and food matrices have been often limited to the use of poorly sustainable materials and complicated preparation/operation.. Here we show a conceptually new strategy for the removal of microplastics and nanoplastics using an olive oil-based emulsion.

    Article Title: β-Cyclodextrin nanosponges for the ocular delivery of therapeutic Micro-RNA in a Mouse model of retinitis Pigmentosa: A proof of concept study.
    Article Snippet: The exploitation of micro-RNA (miR) sequences as therapeutics has become highly attractive for the treatment of several diseases, including those still lacking effective cures such as retinitis pigmentosa (RP).. Interestingly, miR155-5p plays a role in photo-oxidative inflammation in wild-type mice and is up-regulated in rd10 mice showing peak rod degeneration, suggesting its inhibition by the corresponding anti-miR as a viable therapeutic strategy for RP.. However, biomedical application of (anti-)miRs is limited by their oligonucleotide nature, suffering from low solubility and bioavailability along with a very low half-life in vivo due to enzymatic degradation.

    Article Title: Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
    Article Snippet: Slices were manually analyzed under a Nikon Ni-E fluorescence microscope equipped with Nikon DS-Ri2 camera and NIS-Elements C 4.20 (Nikon, Italy).

    Article Title: Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV).
    Article Snippet: The Stimulator of Interferon Genes (STING, also known as MITA/ERYS/MPYS) is an adaptor molecule that plays a crucial role in the RLR pathway and responds to DNA and RNA viruses.. In the present study, we have identified two novel isoforms of STING (the canonical form named as LcSTINGa and its alternative splicing isoform named as LcSTINGb) from teleost Lates calcarifer.. LcSTINGa has an ORF of 1230 bp, encoding a 409 amino acid protein, while its alternative splicing variant, LcSTINGb, features an ORF of 987 bp, encoding 328 amino acids.

    Imaging:

    Article Title: Protein corona as a mediator in antibiotic adsorption onto microplastics: Mechanisms and implications.
    Article Snippet: Microplastics are emerging pollutants capable of adsorbing antibiotics in the environment through interactions mediated by biological molecules such as proteins, ultimately posing risks to human health.. However, direct evidence demonstrating that microplastics and antibiotics form chemical-adsorption products has not been explored.. One key mechanism contributing to their co-exposure risks during their transmission is biofilm formation, particularly the development of a protein corona, which may also serve as a potential virulence mechanism.

    Article Title: Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.
    Article Snippet: Institute of Science Education and Research, Mohali, Punjab, India Correspondence Rajesh Ramachandran, Room 3F10, Academic Block-1, Indian Institute of Science Education and Research, Mohali, Knowledge city, Sector 81, SAS Nagar, Manauli PO, PIN-140306, Mohali, Punjab, India.. Email: rajeshra@iisermohali.ac.in Present address Simran Kaur, Applied Stem Cell, Milpitas, CA, USA.. Vijithkumar Vijayan, Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.

    Article Title: Mechanistic Insights into Dibasic Iminosugars as pH-Selective Pharmacological Chaperones to Stabilize Human α-Galactosidase
    Article Snippet: Images were acquired by using Nikon Ni-E fluorescence microscope and quantified by using ImageJ software.

    Article Title: Trichoderma species from rhizosphere of Oxalis corymbosa release volatile organic compounds inhibiting the seed germination and growth of Echinochloa colona
    Article Snippet: For microscopic morphology, photographs were taken with a Nikon Ni-E fluorescence microscope (Tokyo, Japan) connected to a DP controller digital camera.

    Article Title: A sustainable emulsion for separation and Raman identification of microplastics and nanoplastics
    Article Snippet: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become an issue of increasing concern worldwide.. The currently established approaches and tools for the elimination of the emerging contaminants, in particular microand nano-scale plastic particles, from environmental and food matrices have been often limited to the use of poorly sustainable materials and complicated preparation/operation.. Here we show a conceptually new strategy for the removal of microplastics and nanoplastics using an olive oil-based emulsion.

    Article Title: β-Cyclodextrin nanosponges for the ocular delivery of therapeutic Micro-RNA in a Mouse model of retinitis Pigmentosa: A proof of concept study.
    Article Snippet: The exploitation of micro-RNA (miR) sequences as therapeutics has become highly attractive for the treatment of several diseases, including those still lacking effective cures such as retinitis pigmentosa (RP).. Interestingly, miR155-5p plays a role in photo-oxidative inflammation in wild-type mice and is up-regulated in rd10 mice showing peak rod degeneration, suggesting its inhibition by the corresponding anti-miR as a viable therapeutic strategy for RP.. However, biomedical application of (anti-)miRs is limited by their oligonucleotide nature, suffering from low solubility and bioavailability along with a very low half-life in vivo due to enzymatic degradation.

    Article Title: Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
    Article Snippet: Slices were manually analyzed under a Nikon Ni-E fluorescence microscope equipped with Nikon DS-Ri2 camera and NIS-Elements C 4.20 (Nikon, Italy).

    Article Title: Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV).
    Article Snippet: The Stimulator of Interferon Genes (STING, also known as MITA/ERYS/MPYS) is an adaptor molecule that plays a crucial role in the RLR pathway and responds to DNA and RNA viruses.. In the present study, we have identified two novel isoforms of STING (the canonical form named as LcSTINGa and its alternative splicing isoform named as LcSTINGb) from teleost Lates calcarifer.. LcSTINGa has an ORF of 1230 bp, encoding a 409 amino acid protein, while its alternative splicing variant, LcSTINGb, features an ORF of 987 bp, encoding 328 amino acids.

    Spectrophotometry:

    Article Title: Protein corona as a mediator in antibiotic adsorption onto microplastics: Mechanisms and implications.
    Article Snippet: Microplastics are emerging pollutants capable of adsorbing antibiotics in the environment through interactions mediated by biological molecules such as proteins, ultimately posing risks to human health.. However, direct evidence demonstrating that microplastics and antibiotics form chemical-adsorption products has not been explored.. One key mechanism contributing to their co-exposure risks during their transmission is biofilm formation, particularly the development of a protein corona, which may also serve as a potential virulence mechanism.

    Article Title: Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.
    Article Snippet: Institute of Science Education and Research, Mohali, Punjab, India Correspondence Rajesh Ramachandran, Room 3F10, Academic Block-1, Indian Institute of Science Education and Research, Mohali, Knowledge city, Sector 81, SAS Nagar, Manauli PO, PIN-140306, Mohali, Punjab, India.. Email: rajeshra@iisermohali.ac.in Present address Simran Kaur, Applied Stem Cell, Milpitas, CA, USA.. Vijithkumar Vijayan, Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.

    Article Title: Mechanistic Insights into Dibasic Iminosugars as pH-Selective Pharmacological Chaperones to Stabilize Human α-Galactosidase
    Article Snippet: Images were acquired by using Nikon Ni-E fluorescence microscope and quantified by using ImageJ software.

    Article Title: Trichoderma species from rhizosphere of Oxalis corymbosa release volatile organic compounds inhibiting the seed germination and growth of Echinochloa colona
    Article Snippet: For microscopic morphology, photographs were taken with a Nikon Ni-E fluorescence microscope (Tokyo, Japan) connected to a DP controller digital camera.

    Article Title: A sustainable emulsion for separation and Raman identification of microplastics and nanoplastics
    Article Snippet: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become an issue of increasing concern worldwide.. The currently established approaches and tools for the elimination of the emerging contaminants, in particular microand nano-scale plastic particles, from environmental and food matrices have been often limited to the use of poorly sustainable materials and complicated preparation/operation.. Here we show a conceptually new strategy for the removal of microplastics and nanoplastics using an olive oil-based emulsion.

    Article Title: β-Cyclodextrin nanosponges for the ocular delivery of therapeutic Micro-RNA in a Mouse model of retinitis Pigmentosa: A proof of concept study.
    Article Snippet: The exploitation of micro-RNA (miR) sequences as therapeutics has become highly attractive for the treatment of several diseases, including those still lacking effective cures such as retinitis pigmentosa (RP).. Interestingly, miR155-5p plays a role in photo-oxidative inflammation in wild-type mice and is up-regulated in rd10 mice showing peak rod degeneration, suggesting its inhibition by the corresponding anti-miR as a viable therapeutic strategy for RP.. However, biomedical application of (anti-)miRs is limited by their oligonucleotide nature, suffering from low solubility and bioavailability along with a very low half-life in vivo due to enzymatic degradation.

    Article Title: Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
    Article Snippet: Slices were manually analyzed under a Nikon Ni-E fluorescence microscope equipped with Nikon DS-Ri2 camera and NIS-Elements C 4.20 (Nikon, Italy).

    Article Title: Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV).
    Article Snippet: The Stimulator of Interferon Genes (STING, also known as MITA/ERYS/MPYS) is an adaptor molecule that plays a crucial role in the RLR pathway and responds to DNA and RNA viruses.. In the present study, we have identified two novel isoforms of STING (the canonical form named as LcSTINGa and its alternative splicing isoform named as LcSTINGb) from teleost Lates calcarifer.. LcSTINGa has an ORF of 1230 bp, encoding a 409 amino acid protein, while its alternative splicing variant, LcSTINGb, features an ORF of 987 bp, encoding 328 amino acids.

    Immunostaining:

    Article Title: Protein corona as a mediator in antibiotic adsorption onto microplastics: Mechanisms and implications.
    Article Snippet: Microplastics are emerging pollutants capable of adsorbing antibiotics in the environment through interactions mediated by biological molecules such as proteins, ultimately posing risks to human health.. However, direct evidence demonstrating that microplastics and antibiotics form chemical-adsorption products has not been explored.. One key mechanism contributing to their co-exposure risks during their transmission is biofilm formation, particularly the development of a protein corona, which may also serve as a potential virulence mechanism.

    Article Title: Pten associates with important gene regulatory network to fine-tune Müller glia-mediated zebrafish retina regeneration.
    Article Snippet: Institute of Science Education and Research, Mohali, Punjab, India Correspondence Rajesh Ramachandran, Room 3F10, Academic Block-1, Indian Institute of Science Education and Research, Mohali, Knowledge city, Sector 81, SAS Nagar, Manauli PO, PIN-140306, Mohali, Punjab, India.. Email: rajeshra@iisermohali.ac.in Present address Simran Kaur, Applied Stem Cell, Milpitas, CA, USA.. Vijithkumar Vijayan, Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India.

    Article Title: Mechanistic Insights into Dibasic Iminosugars as pH-Selective Pharmacological Chaperones to Stabilize Human α-Galactosidase
    Article Snippet: Images were acquired by using Nikon Ni-E fluorescence microscope and quantified by using ImageJ software.

    Article Title: Trichoderma species from rhizosphere of Oxalis corymbosa release volatile organic compounds inhibiting the seed germination and growth of Echinochloa colona
    Article Snippet: For microscopic morphology, photographs were taken with a Nikon Ni-E fluorescence microscope (Tokyo, Japan) connected to a DP controller digital camera.

    Article Title: A sustainable emulsion for separation and Raman identification of microplastics and nanoplastics
    Article Snippet: The extensive occurrence of small plastic particles in the environment and our foods, and the resulting hazardous effects to nature and people have become an issue of increasing concern worldwide.. The currently established approaches and tools for the elimination of the emerging contaminants, in particular microand nano-scale plastic particles, from environmental and food matrices have been often limited to the use of poorly sustainable materials and complicated preparation/operation.. Here we show a conceptually new strategy for the removal of microplastics and nanoplastics using an olive oil-based emulsion.

    Article Title: β-Cyclodextrin nanosponges for the ocular delivery of therapeutic Micro-RNA in a Mouse model of retinitis Pigmentosa: A proof of concept study.
    Article Snippet: The exploitation of micro-RNA (miR) sequences as therapeutics has become highly attractive for the treatment of several diseases, including those still lacking effective cures such as retinitis pigmentosa (RP).. Interestingly, miR155-5p plays a role in photo-oxidative inflammation in wild-type mice and is up-regulated in rd10 mice showing peak rod degeneration, suggesting its inhibition by the corresponding anti-miR as a viable therapeutic strategy for RP.. However, biomedical application of (anti-)miRs is limited by their oligonucleotide nature, suffering from low solubility and bioavailability along with a very low half-life in vivo due to enzymatic degradation.

    Article Title: Thalamo-hippocampal pathway regulates incidental memory capacity in mice.
    Article Snippet: Slices were manually analyzed under a Nikon Ni-E fluorescence microscope equipped with Nikon DS-Ri2 camera and NIS-Elements C 4.20 (Nikon, Italy).

    Article Title: Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV).
    Article Snippet: The Stimulator of Interferon Genes (STING, also known as MITA/ERYS/MPYS) is an adaptor molecule that plays a crucial role in the RLR pathway and responds to DNA and RNA viruses.. In the present study, we have identified two novel isoforms of STING (the canonical form named as LcSTINGa and its alternative splicing isoform named as LcSTINGb) from teleost Lates calcarifer.. LcSTINGa has an ORF of 1230 bp, encoding a 409 amino acid protein, while its alternative splicing variant, LcSTINGb, features an ORF of 987 bp, encoding 328 amino acids.



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    96
    Nikon eclipse ni e fluorescence microscope with109
    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.
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    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: Staining, Immunostaining, Immunofluorescence, Imaging, Two Tailed Test

    a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: In Vitro, Inhibition, Immunostaining, Staining, Immunofluorescence, Two Tailed Test