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neuron isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec neuron isolation kit
    Neuron Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neurons/Neuron+Isolation+Kit%2C+mouse/pmc13673058-339-12-16
    Average 96 stars, based on 164 article reviews
    neuron isolation kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Neuronal activity-dependent mechanisms of small cell lung cancer pathogenesis
    Article Snippet: .. Neurons were isolated from the brains of CD1 mice using the Neural Tissue Dissociation Kit - Postnatal Neurons (Miltenyi), followed by the Neuron Isolation Kit, Mouse (Miltenyi) per the manufacturer’s instructions. .. After isolation, 300,000 neurons were plated onto circular glass coverslips (Electron Microscopy Services) pre-treated for 20 min at 37 °C with poly- l -lysine (Sigma) and then 3 h at 37 °C with 5 μg ml −1 mouse laminin (Thermo Fisher).

    Article Title: Caspase-8 expression in CD8 + T cells promotes pathogen restriction in the brain during Toxoplasma gondii infection
    Article Snippet: Serum cytokine levels were detected according to the manufacturer’s instructions using Quantikine enzyme-linked immunosorbent assay (ELISA) (Mouse IL-12 p40 allele specific, R&D Systems, catalog no. M1240) and DuoSet ELISA (Mouse IFN-gamma, R&D Systems, catalog no. DY485). .. Purified cell populations isolated to measure excision of Casp8 were collected according to the manufacturer’s instructions: neurons (Miltenyi Biotech, catalog no. 130-115-390), ACSA2 + astrocytes (Miltenyi Biotech, catalog no. 130-097-678), CD11b + microglia (Miltenyi Biotech, catalog no. 130-093-634), monocytes (Miltenyi Biotech, catalog no. 130-100-629), and CD8α + T cells (Miltenyi Biotech, catalog no. 130-104-075). .. Brain homogenate was homogenized in TRIzol (Thermo Fisher Scientific, catalog no. 15-596-026), and RNA was extracted according to the manufacturer’s protocol. cDNA was then generated using a High-Capacity Reverse Transcription Kit (Applied Biosystems, catalog no. 4374967). qPCR was performed using 2× Taq-based Master Mix (Bioline, catalog no. 21105) and Taq Man gene expression assays (Thermo Fisher Scientific, catalog no. 4331182).

    Article Title: Sex-specific systemic and brain metabolic responses to a standardized ketogenic diet in mice.
    Article Snippet: Then, brains were collected and placed in ice-cold phosphate-buffered saline (PBS) before being dissociated into single-cell suspensions using the Adult Brain Dissociation Kit (130-107- 677, Miltenyi). .. After dissociation, astrocytes were labeled using the ACSA-2 antibody (1:10; 130-097-678, Miltenyi)96, and neurons were isolated via negative selection (130-115-389, Miltenyi). .. RNA extraction from isolated cells was performed using RNeasy Mini Kit (74104, Qiagen).

    Article Title: Neuronal activity-dependent mechanisms of small cell lung cancer pathogenesis.
    Article Snippet: .. Co-culture of SCLC cells with primary mouse neurons Neurons were isolated from the brains of CD1 mice using the Neural Tissue Dissociation Kit - Postnatal Neurons (Miltenyi), followed by the Neuron Isolation Kit, Mouse (Miltenyi) per the manufacturer’s instructions. .. After isolation, 300,000 neurons were plated onto circular glass coverslips (Electron Microscopy Services) pre-treated for 20 min at 37 °C with poly-l-lysine (Sigma) and then 3 h at 37 °C with 5 μg ml−1 mouse laminin (Thermo Fisher).

    Purification:

    Article Title: Caspase-8 expression in CD8 + T cells promotes pathogen restriction in the brain during Toxoplasma gondii infection
    Article Snippet: Serum cytokine levels were detected according to the manufacturer’s instructions using Quantikine enzyme-linked immunosorbent assay (ELISA) (Mouse IL-12 p40 allele specific, R&D Systems, catalog no. M1240) and DuoSet ELISA (Mouse IFN-gamma, R&D Systems, catalog no. DY485). .. Purified cell populations isolated to measure excision of Casp8 were collected according to the manufacturer’s instructions: neurons (Miltenyi Biotech, catalog no. 130-115-390), ACSA2 + astrocytes (Miltenyi Biotech, catalog no. 130-097-678), CD11b + microglia (Miltenyi Biotech, catalog no. 130-093-634), monocytes (Miltenyi Biotech, catalog no. 130-100-629), and CD8α + T cells (Miltenyi Biotech, catalog no. 130-104-075). .. Brain homogenate was homogenized in TRIzol (Thermo Fisher Scientific, catalog no. 15-596-026), and RNA was extracted according to the manufacturer’s protocol. cDNA was then generated using a High-Capacity Reverse Transcription Kit (Applied Biosystems, catalog no. 4374967). qPCR was performed using 2× Taq-based Master Mix (Bioline, catalog no. 21105) and Taq Man gene expression assays (Thermo Fisher Scientific, catalog no. 4331182).

    Saline:

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis
    Article Snippet: .. After rinsing in Dulbecco’s Phosphate Buffered Saline (DPBS), tissues were incubated with the Neural Tissue Dissociation Kit-Postnatal Neurons (Miltenyi Biotec, Bergisch Gladbach, DE, catalog n 130-092-628) according to the manufacturer instruction. ..

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis.
    Article Snippet: .. After rinsing in Dulbecco’s Phosphate Buffered Saline (DPBS), tissues were incubated with the Neural Tissue Dissociation Kit-Postnatal Neurons (Miltenyi Biotec, Bergisch Gladbach, DE, catalog n 130-092-628) according to the manufacturer instruction. ..

    Incubation:

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis
    Article Snippet: .. After rinsing in Dulbecco’s Phosphate Buffered Saline (DPBS), tissues were incubated with the Neural Tissue Dissociation Kit-Postnatal Neurons (Miltenyi Biotec, Bergisch Gladbach, DE, catalog n 130-092-628) according to the manufacturer instruction. ..

    Article Title: Exploiting porphyrin metabolism to inhibit angiogenesis.
    Article Snippet: .. After rinsing in Dulbecco’s Phosphate Buffered Saline (DPBS), tissues were incubated with the Neural Tissue Dissociation Kit-Postnatal Neurons (Miltenyi Biotec, Bergisch Gladbach, DE, catalog n 130-092-628) according to the manufacturer instruction. ..

    RNA Extraction:

    Article Title: Single Cell RNAseq Analysis of Thyroid Hormone Effects on Retinal Glial Cells.
    Article Snippet: Retinal extraction and cell dissociation Retinas were isolated from the eyes of both control and T3-treated mice and placed in Dulbecco’s Modified Eagle Medium (ThermoFisher, Richardson, TX, USA). .. One retina was snap-frozen in liquid nitrogen and kept at −80 °C for RNA extraction, while the other retina underwent cellular dissociation using the Neural Tissue Dissociation Kit-Postnatal Neurons (Miltenyi Biotec, cat# 130-094- 802). .. Retinal samples were rinsed twice in DPBS containing calcium, magnesium (Sigma-Aldrich, cat# D8662), and 0.5% BSA before adding 1960 μL of enzyme mix 1.

    Labeling:

    Article Title: Sex-specific systemic and brain metabolic responses to a standardized ketogenic diet in mice.
    Article Snippet: Then, brains were collected and placed in ice-cold phosphate-buffered saline (PBS) before being dissociated into single-cell suspensions using the Adult Brain Dissociation Kit (130-107- 677, Miltenyi). .. After dissociation, astrocytes were labeled using the ACSA-2 antibody (1:10; 130-097-678, Miltenyi)96, and neurons were isolated via negative selection (130-115-389, Miltenyi). .. RNA extraction from isolated cells was performed using RNeasy Mini Kit (74104, Qiagen).

    Selection:

    Article Title: Sex-specific systemic and brain metabolic responses to a standardized ketogenic diet in mice.
    Article Snippet: Then, brains were collected and placed in ice-cold phosphate-buffered saline (PBS) before being dissociated into single-cell suspensions using the Adult Brain Dissociation Kit (130-107- 677, Miltenyi). .. After dissociation, astrocytes were labeled using the ACSA-2 antibody (1:10; 130-097-678, Miltenyi)96, and neurons were isolated via negative selection (130-115-389, Miltenyi). .. RNA extraction from isolated cells was performed using RNeasy Mini Kit (74104, Qiagen).



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    Image Search Results


    A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and IBA1 (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.

    Journal: bioRxiv

    Article Title: The mitochondrial transaminase GOT2 supports Japanese encephalitis virus replication in neuronal cells

    doi: 10.64898/2026.10.12.758629

    Figure Lengend Snippet: A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and IBA1 (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.

    Article Snippet: The first cocktail included primary neuron antibody MAP2 (catalog #17490-1-AP, Proteintech) conjugated with CoraLite Plus 647 (catalog #KFA003, Proteintech) at 1:100 dilution, activated microglia antibody IBA1 (catalog #10904-1-AP, Proteintech) conjugated with CoraLite Plus 555 (catalog #KFA002, Proteintech) at 1:200 dilution, and JEV NS1 antibody (catalog #GTX633820, GneTex) conjugated with CoraLite Plus 488 (catalog #KFA521, Proteintech) at 1:500 dilution.

    Techniques: Infection, Analysis, Labeling, Quantitative Proteomics, Western Blot, Control, Super Resolution, Microscopy, Tissue, Staining