cell lines hek293t cells atcc crl 11268 primary neuronal cultures (ATCC)
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Cell Lines Hek293t Cells Atcc Crl 11268 Primary Neuronal Cultures, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neuronal+cell+line/293T%2F17/pm41780526-220-102-106
Average 99 stars, based on 12205 article reviews
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Cell Culture:Article Title: Time-Dependent Addition of Neuronal and Schwann Cells Increase Myotube Viability and Length in an In Vitro Tri-culture Model of the Neuromuscular Junction Article Snippet: The neuromuscular junction (NMJ) is a specialized chemical synapse between motor neurons and muscle fibers that enables voluntary movement of somatic muscle.. Study of NMJ physiology and pathology has used various animal models and cell lines to recapitulate critical features of neuromuscular disease and increase our understanding of NMJ disruption.. In vitro co-culture platforms have also been used to evaluate NMJ development using different combinations of motor neurons (MNs), glial Schwann Cells (SCs), skeletal muscle (SKM) cells, and biomaterials to advance translation of regenerative therapies. Article Title: Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments. Article Snippet: .. 50 000 cells of a Article Title: Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth. Article Snippet: .. The Article Title: Implantable Multi-Cross-Linked Membrane-Ionogel Assembly for Reversible Non-Faradaic Neurostimulation. Article Snippet: Neural interfaces play a major role in modulating neural signals for therapeutic purposes.. To meet the demand of conformable neural interfaces for developing bioelectronic medicine, recent studies have focused on the performance of electrical neurostimulators employing soft conductors such as conducting polymers and electronic or ionic conductive hydrogels.. However, faradaic charge injection at the interface of the electrode and nerve tissue causes irreversible gas evolution, oxidation of electrodes, and reduction of biological ions, thus causing undesired tissue damage and electrode degradation. Incubation:Article Title: Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments. Article Snippet: .. 50 000 cells of a Modification:Article Title: Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth. Article Snippet: .. The other:Article Title: Hexavalent Chromium Induces Apoptosis and Autophagy in Human Neurons and Astrocytes via MAPK Pathway Activation. Article Snippet: A human neuronal cell line (SK-N-SH) and a |
![PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated <t>NSC34</t> neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820<x <134.3874] and 7 days [t(2) = −3.390, p = 0.07708, 95 % CI -66.21<x<104.0] post-transfection. (C – F) Transfection of PTEN -siRNA nanoparticles does not affect neuronal morphology. Control and PTEN -siRNA nanoparticle treated neurons have a similar distribution, density, and morphology compared to control neurons (C, D). Higher magnification images showed that all neurons (control and treated) possessed a typical neuronal morphology with several neurites extending from each cell body (E, F). All cells were stained for F-actin (green = phalloidin) and nuclei (blue = DAPI). Scale Bar in (C, D): 100 μm; Scale Bar in (E, F): 50 μm. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8063/pmc12908063/pmc12908063__gr1.jpg)
