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cell lines hek293t cells atcc crl 11268 primary neuronal cultures  (ATCC)


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    ATCC cell lines hek293t cells atcc crl 11268 primary neuronal cultures
    Cell Lines Hek293t Cells Atcc Crl 11268 Primary Neuronal Cultures, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neuronal+cell+line/293T%2F17/pm41780526-220-102-106
    Average 99 stars, based on 12205 article reviews
    cell lines hek293t cells atcc crl 11268 primary neuronal cultures - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Time-Dependent Addition of Neuronal and Schwann Cells Increase Myotube Viability and Length in an In Vitro Tri-culture Model of the Neuromuscular Junction
    Article Snippet: The neuromuscular junction (NMJ) is a specialized chemical synapse between motor neurons and muscle fibers that enables voluntary movement of somatic muscle.. Study of NMJ physiology and pathology has used various animal models and cell lines to recapitulate critical features of neuromuscular disease and increase our understanding of NMJ disruption.. In vitro co-culture platforms have also been used to evaluate NMJ development using different combinations of motor neurons (MNs), glial Schwann Cells (SCs), skeletal muscle (SKM) cells, and biomaterials to advance translation of regenerative therapies.

    Article Title: Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments.
    Article Snippet: .. 50 000 cells of a neuronal cell line (Neuro2A, ATCC) were then seeded on the scaffolds with 2 mL of cell culture medium and incubated for 3 days at 37 °C under a humidified atmosphere containing 5% CO2. ..

    Article Title: Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth.
    Article Snippet: .. The neuronal cell line (Neuro2A, ATCC) was cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin at 37 °C in a humidified atmosphere containing 5% CO2. .. For the cell viability assay, 300 μL of each gel were prepared in an 8- well chamber slide (Lab-Tek, Nunc) as described above.

    Article Title: Implantable Multi-Cross-Linked Membrane-Ionogel Assembly for Reversible Non-Faradaic Neurostimulation.
    Article Snippet: Neural interfaces play a major role in modulating neural signals for therapeutic purposes.. To meet the demand of conformable neural interfaces for developing bioelectronic medicine, recent studies have focused on the performance of electrical neurostimulators employing soft conductors such as conducting polymers and electronic or ionic conductive hydrogels.. However, faradaic charge injection at the interface of the electrode and nerve tissue causes irreversible gas evolution, oxidation of electrodes, and reduction of biological ions, thus causing undesired tissue damage and electrode degradation.

    Incubation:

    Article Title: Wet spinning and radial self-assembly of a carbohydrate low molecular weight gelator into well organized hydrogel filaments.
    Article Snippet: .. 50 000 cells of a neuronal cell line (Neuro2A, ATCC) were then seeded on the scaffolds with 2 mL of cell culture medium and incubated for 3 days at 37 °C under a humidified atmosphere containing 5% CO2. ..

    Modification:

    Article Title: Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth.
    Article Snippet: .. The neuronal cell line (Neuro2A, ATCC) was cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin at 37 °C in a humidified atmosphere containing 5% CO2. .. For the cell viability assay, 300 μL of each gel were prepared in an 8- well chamber slide (Lab-Tek, Nunc) as described above.

    other:

    Article Title: Hexavalent Chromium Induces Apoptosis and Autophagy in Human Neurons and Astrocytes via MAPK Pathway Activation.
    Article Snippet: A human neuronal cell line (SK-N-SH) and a human astrocyte cell line (U-87 MG) were obtained from the American Type Culture Collection (ATCC, USA).



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    PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated <t>NSC34</t> neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820<x <134.3874] and 7 days [t(2) = −3.390, p = 0.07708, 95 % CI -66.21<x<104.0] post-transfection. (C – F) Transfection of PTEN -siRNA nanoparticles does not affect neuronal morphology. Control and PTEN -siRNA nanoparticle treated neurons have a similar distribution, density, and morphology compared to control neurons (C, D). Higher magnification images showed that all neurons (control and treated) possessed a typical neuronal morphology with several neurites extending from each cell body (E, F). All cells were stained for F-actin (green = phalloidin) and nuclei (blue = DAPI). Scale Bar in (C, D): 100 μm; Scale Bar in (E, F): 50 μm. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.
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    Effects of donepezil control solution, donepezil-loaded polymeric NPs, and donepezil-loaded DPPC-based liposomes treatment on ECV-304 <t>and</t> <t>PC-12</t> cells. Cells were treated with increasing concentrations (3.125–50 µg mL −1 ) of each formulation in a time-dependent manner. All formulations maintained cell viability above 80% at concentrations up to 50 µg mL −1 in both cell lines, indicating acceptable biocompatibility. A Two-way ANOVA followed by Tukey's test was applied at each time point independently to compare the effect of different formulations across different concentrations within each cell line. **Significant with respect to the control ( p < 0.01), as shown by two-way ANOVA. The data is reported as mean ± SEM ( n = 3).
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    Image Search Results


    PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated NSC34 neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820<x <134.3874] and 7 days [t(2) = −3.390, p = 0.07708, 95 % CI -66.21<x<104.0] post-transfection. (C – F) Transfection of PTEN -siRNA nanoparticles does not affect neuronal morphology. Control and PTEN -siRNA nanoparticle treated neurons have a similar distribution, density, and morphology compared to control neurons (C, D). Higher magnification images showed that all neurons (control and treated) possessed a typical neuronal morphology with several neurites extending from each cell body (E, F). All cells were stained for F-actin (green = phalloidin) and nuclei (blue = DAPI). Scale Bar in (C, D): 100 μm; Scale Bar in (E, F): 50 μm. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.

    Journal: Bioactive Materials

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury

    doi: 10.1016/j.bioactmat.2026.01.022

    Figure Lengend Snippet: PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated NSC34 neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.

    Article Snippet: NSC34 neuronal culture and transfections: NSC34 neurons (Cedarlane Cat No. CLU140), a hybrid cell line produced by the fusion of motor neuron enriched embryonic mouse spinal cord cells with mouse neuroblastoma cells, were cultured in growth medium Dulbecco's Modified Eagle Medium (DMEM; Sigma-Aldrich, Ireland) supplemented with 10 % fetal bovine serum (FBS; Labtech UK), 1 % (v/v) L-Glutamine (Sigma-Aldrich, Ireland), and 1 % (v/v) Penicillin- Streptomycin Solution (Sigma-Aldrich, Ireland) in a T-175 cell culture flask (37 °C, 5 % CO 2 ).

    Techniques: Transfection, Knockdown, One-tailed Test, Expressing, Activity Assay, Control, Staining

    Scaffold-mediated siRNA delivery does not affect the viability of neurons, results in prolonged downregulation of PTEN, and stimulates increased expression of BCL2 and GAP43 mRNA . (A) The metabolic activity of NSC34 neurons cultured on PTEN -siRNA activated scaffolds peaked by day 3 post-transfection [t(2) = 4.0599, p = 0.05565, 95 % CI 93.954<x<308.3], decreased by day 7 [t(2) = −15.068, p = 0.004375, 95 % CI 49.7157<x<72.05] and was similar to control levels by day 21 [t(2) = −0.1042, p = 0.9265, 95 % CI 83.23<x<116.0]. Metabolic activity normalization was performed relative to the untreated cells. (B) LDH assay revealed a consistent but small increase in cell stress over a span of 21 days. (C – D) Analysis of PTEN gene expression in scaffold transfected neurons over 21 days showed a significant decrease in expression levels [t(4) = −3.2927, p = 0.01507; one-tailed] 3 days after transfection that was then followed by a gradual return toward untreated control levels denoted by the blue regression line which approaches the red untreated control line after 21 days. (E – F) The change in BCL2 expression 3-, 7-, and 21-days post-transfection showed a significant initial elevation [t(4) = 2.092, p = 0.05227, one-tailed], followed by a gradual reduction converging toward untreated control levels. (G – H). Similarly, GAP43 expression was characterised by of an initial significant rise [t(4) = 2.1748, p = 0.04765, one-tailed] in levels that was followed by a decrease over the 21-day culture period. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.

    Journal: Bioactive Materials

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury

    doi: 10.1016/j.bioactmat.2026.01.022

    Figure Lengend Snippet: Scaffold-mediated siRNA delivery does not affect the viability of neurons, results in prolonged downregulation of PTEN, and stimulates increased expression of BCL2 and GAP43 mRNA . (A) The metabolic activity of NSC34 neurons cultured on PTEN -siRNA activated scaffolds peaked by day 3 post-transfection [t(2) = 4.0599, p = 0.05565, 95 % CI 93.954 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.

    Article Snippet: NSC34 neuronal culture and transfections: NSC34 neurons (Cedarlane Cat No. CLU140), a hybrid cell line produced by the fusion of motor neuron enriched embryonic mouse spinal cord cells with mouse neuroblastoma cells, were cultured in growth medium Dulbecco's Modified Eagle Medium (DMEM; Sigma-Aldrich, Ireland) supplemented with 10 % fetal bovine serum (FBS; Labtech UK), 1 % (v/v) L-Glutamine (Sigma-Aldrich, Ireland), and 1 % (v/v) Penicillin- Streptomycin Solution (Sigma-Aldrich, Ireland) in a T-175 cell culture flask (37 °C, 5 % CO 2 ).

    Techniques: Expressing, Activity Assay, Cell Culture, Transfection, Control, Lactate Dehydrogenase Assay, Gene Expression, One-tailed Test

    Effects of donepezil control solution, donepezil-loaded polymeric NPs, and donepezil-loaded DPPC-based liposomes treatment on ECV-304 and PC-12 cells. Cells were treated with increasing concentrations (3.125–50 µg mL −1 ) of each formulation in a time-dependent manner. All formulations maintained cell viability above 80% at concentrations up to 50 µg mL −1 in both cell lines, indicating acceptable biocompatibility. A Two-way ANOVA followed by Tukey's test was applied at each time point independently to compare the effect of different formulations across different concentrations within each cell line. **Significant with respect to the control ( p < 0.01), as shown by two-way ANOVA. The data is reported as mean ± SEM ( n = 3).

    Journal: RSC Advances

    Article Title: Comparative evaluation of donepezil-loaded polymeric and liposomal nanoparticles for Alzheimer's disease: biocompatibility, drug release kinetics, and cellular uptake study

    doi: 10.1039/d6ra00929h

    Figure Lengend Snippet: Effects of donepezil control solution, donepezil-loaded polymeric NPs, and donepezil-loaded DPPC-based liposomes treatment on ECV-304 and PC-12 cells. Cells were treated with increasing concentrations (3.125–50 µg mL −1 ) of each formulation in a time-dependent manner. All formulations maintained cell viability above 80% at concentrations up to 50 µg mL −1 in both cell lines, indicating acceptable biocompatibility. A Two-way ANOVA followed by Tukey's test was applied at each time point independently to compare the effect of different formulations across different concentrations within each cell line. **Significant with respect to the control ( p < 0.01), as shown by two-way ANOVA. The data is reported as mean ± SEM ( n = 3).

    Article Snippet: The human endothelial-like cell line ECV-304 and the immortalized rat-derived neuronal-like cell line PC-12 were purchased from ATCC.

    Techniques: Control, Liposomes, Formulation

    In vitro cellular uptake of Rhodamine labelled DPPC-based liposomes (red) and FITC-labelled PCL/PVA nanoparticles (green) by ECV-304 and PC-12 cells after 24 hours of incubation period. Co-localization of the red fluorescence (liposomes) or green fluorescence (PCL/PVA NPs) in proximity to the blue DAPI-stained nuclei confirms the association of nanoparticles with ECV-304 and PC-12 cells, demonstrating either internalization or surface binding. Fluorescence microscopy was performed at 50× magnification; scale bar = 25 µm (consistent across all images).

    Journal: RSC Advances

    Article Title: Comparative evaluation of donepezil-loaded polymeric and liposomal nanoparticles for Alzheimer's disease: biocompatibility, drug release kinetics, and cellular uptake study

    doi: 10.1039/d6ra00929h

    Figure Lengend Snippet: In vitro cellular uptake of Rhodamine labelled DPPC-based liposomes (red) and FITC-labelled PCL/PVA nanoparticles (green) by ECV-304 and PC-12 cells after 24 hours of incubation period. Co-localization of the red fluorescence (liposomes) or green fluorescence (PCL/PVA NPs) in proximity to the blue DAPI-stained nuclei confirms the association of nanoparticles with ECV-304 and PC-12 cells, demonstrating either internalization or surface binding. Fluorescence microscopy was performed at 50× magnification; scale bar = 25 µm (consistent across all images).

    Article Snippet: The human endothelial-like cell line ECV-304 and the immortalized rat-derived neuronal-like cell line PC-12 were purchased from ATCC.

    Techniques: In Vitro, Liposomes, Incubation, Fluorescence, Staining, Binding Assay, Microscopy

    Quantitative analysis of cellular uptake of rhodamine-labelled DPPC-based liposomes and FITC-labelled PCL/PVA NPs along with their respective free dye controls by ECV-304 and PC-12 cell lines. Both formulations showed significantly higher uptake than their respective free dye controls. PCL/PVA nanoparticles exhibited significantly higher uptake than liposomes in ECV-304 cells. Results are expressed as mean ± SD. Statistical significance was determined using a one-way ANOVA followed by Tukey's test to compare liposomes and nanoparticles uptake against their respective controls and denoted as follows: P < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).

    Journal: RSC Advances

    Article Title: Comparative evaluation of donepezil-loaded polymeric and liposomal nanoparticles for Alzheimer's disease: biocompatibility, drug release kinetics, and cellular uptake study

    doi: 10.1039/d6ra00929h

    Figure Lengend Snippet: Quantitative analysis of cellular uptake of rhodamine-labelled DPPC-based liposomes and FITC-labelled PCL/PVA NPs along with their respective free dye controls by ECV-304 and PC-12 cell lines. Both formulations showed significantly higher uptake than their respective free dye controls. PCL/PVA nanoparticles exhibited significantly higher uptake than liposomes in ECV-304 cells. Results are expressed as mean ± SD. Statistical significance was determined using a one-way ANOVA followed by Tukey's test to compare liposomes and nanoparticles uptake against their respective controls and denoted as follows: P < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).

    Article Snippet: The human endothelial-like cell line ECV-304 and the immortalized rat-derived neuronal-like cell line PC-12 were purchased from ATCC.

    Techniques: Liposomes