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neuron isolation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec neuron isolation kit
    Neuron Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neuron+isolation+kit/Neuron+Isolation+Kit%2C+mouse/pmc13673058-339-12-16
    Average 96 stars, based on 164 article reviews
    neuron isolation kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Selection:

    Article Title: Neuron‐Derived Vesicular circMagi1 Restrains Vascular Repair After Ischemic Stroke by Promoting TRIM65‐Mediated ANXA2 Degradation
    Article Snippet: Single‐cell suspensions were prepared using the Neural Tissue Dissociation Kit (130‐093‐231, Miltenyi Biotec) for embryos and the Adult Brain Dissociation Kit (130‐107‐677, Miltenyi Biotec) for adult mice according to the manufacturer's instructions. .. A negative selection strategy was employed to deplete non‐neuronal lineages using the Neuron Isolation Kit (130‐115‐389, Miltenyi Biotec). ..

    Article Title: High glucose impairs cognitive function through Creb3 O-GlcNAcylation and increased lactate production.
    Article Snippet: The high glucose levels characteristic of diabetes can lead to increases in glucose metabolism through the process of glycolysis, resulting in greater production of lactate and in a monosaccharidebased posttranslational modification called OGlcNAcylation.. Here, we identified OGlcNAcylation and lactate production as the molecular mechanisms underlying high glucose–induced cognitive impairment, a prevalent complication of diabetes.. A prospective observational study revealed that elevated plasma concentrations of lactate were an independent risk factor for predicting mild cognitive impairment in patients with diabetes.

    Isolation:

    Article Title: Neuron‐Derived Vesicular circMagi1 Restrains Vascular Repair After Ischemic Stroke by Promoting TRIM65‐Mediated ANXA2 Degradation
    Article Snippet: Single‐cell suspensions were prepared using the Neural Tissue Dissociation Kit (130‐093‐231, Miltenyi Biotec) for embryos and the Adult Brain Dissociation Kit (130‐107‐677, Miltenyi Biotec) for adult mice according to the manufacturer's instructions. .. A negative selection strategy was employed to deplete non‐neuronal lineages using the Neuron Isolation Kit (130‐115‐389, Miltenyi Biotec). ..

    Article Title: High glucose impairs cognitive function through Creb3 O-GlcNAcylation and increased lactate production.
    Article Snippet: The high glucose levels characteristic of diabetes can lead to increases in glucose metabolism through the process of glycolysis, resulting in greater production of lactate and in a monosaccharidebased posttranslational modification called OGlcNAcylation.. Here, we identified OGlcNAcylation and lactate production as the molecular mechanisms underlying high glucose–induced cognitive impairment, a prevalent complication of diabetes.. A prospective observational study revealed that elevated plasma concentrations of lactate were an independent risk factor for predicting mild cognitive impairment in patients with diabetes.

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Article Title: Targeting TLR9 reduces chronic stress-associated neuroinflammation and improves social behavior in a mouse model
    Article Snippet: .. The flow-through containing unlabeled cells were subsequently used for neuronal isolation using the Neuron Isolation Kit (Cat. No. 130-115-389, Miltenyi Biotec), following the manufacturer’s protocol. ..

    Article Title: HMGCS2-dependent β-OHB/H3K9bhb ameliorates synaptic plasticity and cognition in Alzheimer's disease.
    Article Snippet: .. According to the manufacturer’s instructions, mouse neurons were isolated using the adult neuron isolation kit (130-126-603, Miltenyi Biotec)23. ..

    Article Title: Aberrant expression of the MID1 protein in neurons of Huntington’s disease brain
    Article Snippet: .. The Anti-ACSA-2 MicroBead Kit (130-097-678), the CD11b MicroBeads Kit (130-093-634), and the Neuron Isolation Kit (130-097-678) from Miltenyi Biotech were used for MACS. ..

    Article Title: Microenvironment T-Type calcium channels regulate neuronal and glial processes in tumor cells to promote glioblastoma growth.
    Article Snippet: Microenvironment T-Type calcium channels regulate neuronal and glial processes in tumor 1 cells to promote glioblastoma growth 2 3 Collin J. Dube, Ying Zhang, Shekhar Saha, Tyler C J Deutsch, Matthew Yorek, Michelle Lai, 4 Myron K. Gibert, Miguel Escalante, Kadie Hudson, Doris Wong, Pawel Marcinkiewicz, Ulas 5 Yener, Yunan Sun, Esther Xu, Aditya Sorot, Elizabeth Mulcahy, Benjamin Kefas, Farina 6 Hanif, Fadilla Guessous, Ashley Vernon, David Schiff, Hui Zong, Benjamin Purow, 7 Eric Holland, Swapnil Sonkusare, Harald Sontheimer, Manoj K. Patel, Roger 8 Abounader 9 10 Department of Microbiology, Immunology & Cancer Biology, University of Virginia, Charlottesville, 11

    Article Title: Senolytic treatment with dasatinib and quercetin selectively improves cardiac autonomic balance in obesity.
    Article Snippet: .. Neuron and glia isolation Mouse neurons and glial cells (non-neuronal cells) were isolated from whole brainstem suspensions using the Neuron Isolation Kit (130-098-754; Miltenyi Biotec, San Diego, CA, USA) per the manufacturer’s instructions. ..

    Single Cell:

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Suspension:

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Incubation:

    Article Title: DNMT3A drives mitochondrial dysfunction in intracerebral hemorrhage via PGC-1α promoter hypermethylation.
    Article Snippet: .. The single-cell suspension was incubated with 10 μL of biotinconjugated anti-neuron-specific antibodies (Neuron Isolation Kit, Miltenyi Biotec, 130- 115-390) for 15 min at 4°C, followed by 20 μL of anti-biotin magnetic microbeads for an additional 15 min at 4°C. .. Cells were then loaded onto LS columns (Miltenyi Biotec) placed in a magnetic field.

    Magnetic Cell Separation:

    Article Title: Aberrant expression of the MID1 protein in neurons of Huntington’s disease brain
    Article Snippet: .. The Anti-ACSA-2 MicroBead Kit (130-097-678), the CD11b MicroBeads Kit (130-093-634), and the Neuron Isolation Kit (130-097-678) from Miltenyi Biotech were used for MACS. ..

    Magnetic Resonance Imaging:

    Article Title: Microenvironment T-Type calcium channels regulate neuronal and glial processes in tumor cells to promote glioblastoma growth.
    Article Snippet: Microenvironment T-Type calcium channels regulate neuronal and glial processes in tumor 1 cells to promote glioblastoma growth 2 3 Collin J. Dube, Ying Zhang, Shekhar Saha, Tyler C J Deutsch, Matthew Yorek, Michelle Lai, 4 Myron K. Gibert, Miguel Escalante, Kadie Hudson, Doris Wong, Pawel Marcinkiewicz, Ulas 5 Yener, Yunan Sun, Esther Xu, Aditya Sorot, Elizabeth Mulcahy, Benjamin Kefas, Farina 6 Hanif, Fadilla Guessous, Ashley Vernon, David Schiff, Hui Zong, Benjamin Purow, 7 Eric Holland, Swapnil Sonkusare, Harald Sontheimer, Manoj K. Patel, Roger 8 Abounader 9 10 Department of Microbiology, Immunology & Cancer Biology, University of Virginia, Charlottesville, 11

    Immunohistochemistry:

    Article Title: Microenvironment T-Type calcium channels regulate neuronal and glial processes in tumor cells to promote glioblastoma growth.
    Article Snippet: Microenvironment T-Type calcium channels regulate neuronal and glial processes in tumor 1 cells to promote glioblastoma growth 2 3 Collin J. Dube, Ying Zhang, Shekhar Saha, Tyler C J Deutsch, Matthew Yorek, Michelle Lai, 4 Myron K. Gibert, Miguel Escalante, Kadie Hudson, Doris Wong, Pawel Marcinkiewicz, Ulas 5 Yener, Yunan Sun, Esther Xu, Aditya Sorot, Elizabeth Mulcahy, Benjamin Kefas, Farina 6 Hanif, Fadilla Guessous, Ashley Vernon, David Schiff, Hui Zong, Benjamin Purow, 7 Eric Holland, Swapnil Sonkusare, Harald Sontheimer, Manoj K. Patel, Roger 8 Abounader 9 10 Department of Microbiology, Immunology & Cancer Biology, University of Virginia, Charlottesville, 11



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