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Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The <t>transfection</t> efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.
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Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The transfection efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway

doi:

Figure Lengend Snippet: Effects of ELF1 on the proliferation and apoptosis of PC cells. A. The transfection efficiency of ELF1 overexpression plasmid and si-ELF1 was detected by Western blot. B. The effects of ELF1 overexpression or knockdown on the viability of Capan-2 and PANC-1 cells were detected by CCK-8 assay. C. The effects of ELF1 overexpression or knockdown on the proliferation of Capan-2 and PANC-1 cells were detected by EdU assay (scale bars =50 μm). D. The effects of ELF1 overexpression or knockdown on the apoptosis of Capan-2 and PANC-1 cells were detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China).

Techniques: Transfection, Over Expression, Plasmid Preparation, Western Blot, Knockdown, CCK-8 Assay, EdU Assay, Flow Cytometry

ELF1 interferes with cell proliferation and apoptosis by targeting DCLK1. The ELF1 overexpression plasmid and si-DCLK1 were co-transfected into Capan-2 cells, and the si-ELF1 and DCLC1 overexpression plasmids were co-transfected into PANC-1 cells. A. DCLK1 mRNA expression was probed by qRT-PCR after transfection. B, C. The viability of Capan-2 and PANC-1 cells was detected by CCK-8 and EdU assays. D. The apoptosis of Capan-2 and PANC-1 cells was detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Journal: American Journal of Cancer Research

Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway

doi:

Figure Lengend Snippet: ELF1 interferes with cell proliferation and apoptosis by targeting DCLK1. The ELF1 overexpression plasmid and si-DCLK1 were co-transfected into Capan-2 cells, and the si-ELF1 and DCLC1 overexpression plasmids were co-transfected into PANC-1 cells. A. DCLK1 mRNA expression was probed by qRT-PCR after transfection. B, C. The viability of Capan-2 and PANC-1 cells was detected by CCK-8 and EdU assays. D. The apoptosis of Capan-2 and PANC-1 cells was detected by flow cytometry. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China).

Techniques: Over Expression, Plasmid Preparation, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry