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negative control strain s aureus atcc 25904  (ATCC)


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    ATCC negative control strain s aureus atcc 25904
    Negative Control Strain S Aureus Atcc 25904, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/negative+control+strains/Staphylococcus+aureus+subsp%2E+aureus+Rosenbach/pmc12464783__oc5c00562_si_001-56-24-29
    Average 99 stars, based on 1224 article reviews
    negative control strain s aureus atcc 25904 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Control:

    Article Title: Epidemiology and Antibiotic Resistance Patterns of Klebsiella pneumoniae Infections Among Female Patients of a Long-Term Care Hospital in Saudi Arabia
    Article Snippet: .. To ensure test reliability, quality control measures included the use of positive and negative control strains ( K. pneumoniae ATCC 700603 for ESBL, ATCC BAA-1705 for CRE, and E. coli ATCC 25922 as a negative control). ..

    Negative Control:

    Article Title: Epidemiology and Antibiotic Resistance Patterns of Klebsiella pneumoniae Infections Among Female Patients of a Long-Term Care Hospital in Saudi Arabia
    Article Snippet: .. To ensure test reliability, quality control measures included the use of positive and negative control strains ( K. pneumoniae ATCC 700603 for ESBL, ATCC BAA-1705 for CRE, and E. coli ATCC 25922 as a negative control). ..

    Article Title: Risk factors associated with community colonization of extended-spectrum cephalosporin-resistant Enterobacterales from an antibiotic resistance in communities and hospitals (ARCH) study, Guatemala.
    Article Snippet: Stool samples were collected in stool cups, transported to a local laboratory, and streaked onto CHROMagarTM ESBL as well as MacConkey agar (Hardy Diagnostics, CA) as a positive control for gram-negative bacteria, followed by overnight incubation at 37 °C. .. Every batch of CHROMagarTM media was tested with positive control strains (ATCC BAA-2469 and ATCC 700603) and negative control strains (ATCC 29212 and ATCC 25922). ..

    Article Title: Prevalence and antifungal susceptibility of gastrointestinal candidiasis among diabetic patients: A cross-sectional study
    Article Snippet: Fasting blood glucose levels were tested using a point-of-care Accu-chek glucose meter (Roche Diabetes Care, Inc., Ängelholm, Sweden) and glycated hemoglobin levels were determined using an automated glycated hemoglobin 501 analyser (HemoCue AB, Ängelholm, Sweden). .. Growth testing for all culture media was performed using reference Candida strains ( Candida albicans ATCC 10231, Candida glabrata ATCC 15126, Candida krusei ATCC 24408, Candida tropicalis ATCC 750) and negative control strains ( Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923) as recommended by the manufacturers. ..

    Article Title: Comparison of four β-glucuronidase and β-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms in water.
    Article Snippet: The first filter was incubated on MI agar (MI; BD, Franklin Lakes, NJ, USA), the secondfilter was incubated onChromocult coliform® agar (Chromocult coliform®, Merk KGaA, Darmstadt, Germany), and the third filter was incubated on DC with BCIG agar (DCþBCIG; Neogen Corporation, Lansing, MI, USA) for 24± 2 h at 35.0± 0.5 WC, before determining colony counts and colour. .. Each preparation of MI, Chromocult coliform®, and DCþBCIG plates was tested for performance using positive and negative control strains (Enterobacter aerogenes ATCC 13048, E. coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853), as recommended by the manufacturer’s labeled instructions and the USEPA microbiology methods manual. ..

    Article Title: Risk factors associated with community colonization of extended-spectrum cephalosporin-resistant Enterobacterales from an antibiotic resistance in communities and hospitals (ARCH) study, Guatemala
    Article Snippet: Stool samples were collected in stool cups, transported to a local laboratory, and streaked onto CHROMagarTM ESBL as well as MacConkey agar (Hardy Diagnostics, CA) as a positive control for gram-negative bacteria, followed by overnight incubation at 37 °C. .. Every batch of CHROMagarTM media was tested with positive control strains (ATCC BAA-2469 and ATCC 700603) and negative control strains (ATCC 29212 and ATCC 25922). ..

    Article Title: Molecular Detection of bla OXA-48 Carbapenemase in Uropathogenic Klebsiella pneumoniae Strains from Suez Canal University Hospital
    Article Snippet: .. Positive and
    negative control strains (K. pneumoniae ATCC BAA-1705 and K. pneumoniae ATCC BAA-1706 respectively) were inoculated in the same way on each plate. ..

    Article Title: Lytic Bacteriophage Screening Strategies for Multidrug-Resistant Bloodstream Infections in a Burn Intensive Care Unit
    Article Snippet: .. One or two Klebsiella. pneumoniae colonies and negative control strains (ATCC 25922) which had been grown in the agar plate overnight were selected using a 10 μg inoculating loup and spread from the area surrounding the strip to the edge of the plate (each line was equal to or longer than 20 mm). ..

    Article Title: Magnetic relaxation switch immunosensor for the rapid detection of the foodborne pathogen Salmonella enterica in milk samples
    Article Snippet: .. Specificity of the MRS immunosensor The target strain S. enterica (ATCC 14028) and the other negative control strains (ATCC 12022, ATCC 13312, ATCC 25922, and ATCC 27217) were separately tested by using the proposed immunosensor. ..

    Positive Control:

    Article Title: Risk factors associated with community colonization of extended-spectrum cephalosporin-resistant Enterobacterales from an antibiotic resistance in communities and hospitals (ARCH) study, Guatemala.
    Article Snippet: Stool samples were collected in stool cups, transported to a local laboratory, and streaked onto CHROMagarTM ESBL as well as MacConkey agar (Hardy Diagnostics, CA) as a positive control for gram-negative bacteria, followed by overnight incubation at 37 °C. .. Every batch of CHROMagarTM media was tested with positive control strains (ATCC BAA-2469 and ATCC 700603) and negative control strains (ATCC 29212 and ATCC 25922). ..

    Article Title: Risk factors associated with community colonization of extended-spectrum cephalosporin-resistant Enterobacterales from an antibiotic resistance in communities and hospitals (ARCH) study, Guatemala
    Article Snippet: Stool samples were collected in stool cups, transported to a local laboratory, and streaked onto CHROMagarTM ESBL as well as MacConkey agar (Hardy Diagnostics, CA) as a positive control for gram-negative bacteria, followed by overnight incubation at 37 °C. .. Every batch of CHROMagarTM media was tested with positive control strains (ATCC BAA-2469 and ATCC 700603) and negative control strains (ATCC 29212 and ATCC 25922). ..

    Labeling:

    Article Title: Comparison of four β-glucuronidase and β-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms in water.
    Article Snippet: The first filter was incubated on MI agar (MI; BD, Franklin Lakes, NJ, USA), the secondfilter was incubated onChromocult coliform® agar (Chromocult coliform®, Merk KGaA, Darmstadt, Germany), and the third filter was incubated on DC with BCIG agar (DCþBCIG; Neogen Corporation, Lansing, MI, USA) for 24± 2 h at 35.0± 0.5 WC, before determining colony counts and colour. .. Each preparation of MI, Chromocult coliform®, and DCþBCIG plates was tested for performance using positive and negative control strains (Enterobacter aerogenes ATCC 13048, E. coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853), as recommended by the manufacturer’s labeled instructions and the USEPA microbiology methods manual. ..

    Stripping Membranes:

    Article Title: Lytic Bacteriophage Screening Strategies for Multidrug-Resistant Bloodstream Infections in a Burn Intensive Care Unit
    Article Snippet: .. One or two Klebsiella. pneumoniae colonies and negative control strains (ATCC 25922) which had been grown in the agar plate overnight were selected using a 10 μg inoculating loup and spread from the area surrounding the strip to the edge of the plate (each line was equal to or longer than 20 mm). ..



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    Image Search Results


    . Top panel: schematic representation of a 48 hpf embryo (reproduced with modifications from [16]); red line = aorta (from which hematopoietic stem cell precursors emerge); blue line = vein (showing particularly the vein plexus, constituting the CHT). The embryo used expresses eGFP under the control of the vascular kdrl promotor [ Tg(kdrl: eGFP) fish line]. Bottom panels: spinning disk confocal images obtained either in the trunk region (left: AGM) or in the tail region (right: CHT). Images shown were obtained either from maximal z-projections of z-stacks or from single z-sections with 3× magnification of regions in white boxes. Green channel: vascular cells expressing soluble eGFP [aortic and vein endothelial cells, including cells of the hemogenic endothelium (HE; white arrows) in the trunk and tail regions] as well as newly born HSPCs (white asterisks). Magenta: RNAscope spots indicating expression of cmyb mRNAs in the HE and in HSPCs. Note the presence of RNAscope spots in the trunk (top left image) and in the gut region (magenta arrows) that indicate the potential expression of cmyb in more differentiated patrolling immune cells. AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: . Top panel: schematic representation of a 48 hpf embryo (reproduced with modifications from [16]); red line = aorta (from which hematopoietic stem cell precursors emerge); blue line = vein (showing particularly the vein plexus, constituting the CHT). The embryo used expresses eGFP under the control of the vascular kdrl promotor [ Tg(kdrl: eGFP) fish line]. Bottom panels: spinning disk confocal images obtained either in the trunk region (left: AGM) or in the tail region (right: CHT). Images shown were obtained either from maximal z-projections of z-stacks or from single z-sections with 3× magnification of regions in white boxes. Green channel: vascular cells expressing soluble eGFP [aortic and vein endothelial cells, including cells of the hemogenic endothelium (HE; white arrows) in the trunk and tail regions] as well as newly born HSPCs (white asterisks). Magenta: RNAscope spots indicating expression of cmyb mRNAs in the HE and in HSPCs. Note the presence of RNAscope spots in the trunk (top left image) and in the gut region (magenta arrows) that indicate the potential expression of cmyb in more differentiated patrolling immune cells. AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Control, Expressing, RNAscope

    Spinning disk confocal images obtained with a 5 dpf larva [ Tg(kdrl:eGFP ) fish line] expressing eGFP in endothelial cells under the control of the vascular kdrl promotor. Images show the pronephros region with a maximal z-projection (left) of a z-stack encompassing a total of 240 sections, each interspaced by 0.5 μm, and z-sections (right: images of sections 52/240, 86/240, 186/240, and 222/240 from top to bottom, respectively). The cmyb RNAscope signals (magenta) correspond to hematopoietic stem and progenitor cells (HSPCs) accumulated in the peri-glomerular region (white arrow), surrounded by vessels. Note the more distant localization of cmyb signals (magenta arrows, hypothetically in HSPCs) along vessels (green arrows). This unveils the potential complexity of the pronephros niche. ISV = intersegmental vessel. This z-stack is also visualized in and reconstituted in 3D using Imaris in . Note also the nonspecific capture of the dye in the notochord, which facilitated the localization of the glomerulus. Scale bars = 20 μm.

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: Spinning disk confocal images obtained with a 5 dpf larva [ Tg(kdrl:eGFP ) fish line] expressing eGFP in endothelial cells under the control of the vascular kdrl promotor. Images show the pronephros region with a maximal z-projection (left) of a z-stack encompassing a total of 240 sections, each interspaced by 0.5 μm, and z-sections (right: images of sections 52/240, 86/240, 186/240, and 222/240 from top to bottom, respectively). The cmyb RNAscope signals (magenta) correspond to hematopoietic stem and progenitor cells (HSPCs) accumulated in the peri-glomerular region (white arrow), surrounded by vessels. Note the more distant localization of cmyb signals (magenta arrows, hypothetically in HSPCs) along vessels (green arrows). This unveils the potential complexity of the pronephros niche. ISV = intersegmental vessel. This z-stack is also visualized in and reconstituted in 3D using Imaris in . Note also the nonspecific capture of the dye in the notochord, which facilitated the localization of the glomerulus. Scale bars = 20 μm.

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Expressing, Control, RNAscope

    Spinning disk confocal images obtained with a 5 dpf larva [ Tg(runx1+23:eGFP ) fish line] expressing eGFP in HSPCs under the control of the runx1+23 enhancer. Panels are constituted from images obtained either in the trunk region (left: AGM) or in the tail (right: CHT), either from maximum z-projections of z-stacks or from single z-sections with 2× magnification of regions in white boxes, highlighting hematopoietic clusters (dashed lines). RNAscope signals (magenta) colocalize in the majority with HSPCs (see also Figure 7 and for 3D reconstitution). AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: Spinning disk confocal images obtained with a 5 dpf larva [ Tg(runx1+23:eGFP ) fish line] expressing eGFP in HSPCs under the control of the runx1+23 enhancer. Panels are constituted from images obtained either in the trunk region (left: AGM) or in the tail (right: CHT), either from maximum z-projections of z-stacks or from single z-sections with 2× magnification of regions in white boxes, highlighting hematopoietic clusters (dashed lines). RNAscope signals (magenta) colocalize in the majority with HSPCs (see also Figure 7 and for 3D reconstitution). AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Expressing, Control, RNAscope

    All data shown are extracted from a single caudal hematopoietic tissue (CHT) z-stack showing the expression of cmyb (RNAscope, spots in magenta) in hematopoietic cells expressing eGFP under the control of the runx1+23 enhancer (the same 5 dpf larva and images as in Figure 6). (A) File conversion with Imaris File Converter. (B) Imaris 3D visualization in surpass mode. (C) Step-by-step workflow of cell segmentation, as detailed in Data Analysis, Section B. 3D cells and RNAscope signal segmentation with Imaris – Cell Biologist package .

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: All data shown are extracted from a single caudal hematopoietic tissue (CHT) z-stack showing the expression of cmyb (RNAscope, spots in magenta) in hematopoietic cells expressing eGFP under the control of the runx1+23 enhancer (the same 5 dpf larva and images as in Figure 6). (A) File conversion with Imaris File Converter. (B) Imaris 3D visualization in surpass mode. (C) Step-by-step workflow of cell segmentation, as detailed in Data Analysis, Section B. 3D cells and RNAscope signal segmentation with Imaris – Cell Biologist package .

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Expressing, RNAscope, Control

    . Top panel: schematic representation of a 5 dpf larva (reproduced with modifications from [16]); red line = aorta; blue line = vein. The larva used expresses eGFP under the control of the vascular kdrl promotor [ Tg(kdrl: eGFP) fish line]. Bottom panels: spinning disk confocal images obtained either in the trunk region (left: AGM) or in the tail region (right: CHT). Images shown were obtained either from maximal z-projections of z-stacks or from single z-sections with 1.5× magnification of regions in white boxes. Green channel: vascular cells expressing soluble eGFP (aortic and veinous endothelial cells in the trunk and tail regions). Magenta: RNAscope spots indicating expression of cmyb mRNAs in HSPCs. Note that RNAscope signals cannot be superposed to individual cells because HSPCs are no longer expressing eGFP driven by the vascular kdrl promoter, as is the case in 48 hpf embryos (owing to distant timing from their emergence, half-life of eGFP, and division cycles diluting the fluorescent protein). Note the structural evolution of the vein niche, notably in the CHT, in comparison with the 48 hpf embryo shown in Figure 3, and the relatively regular positioning of hematopoietic clusters (delimited by dashed lines in the bottom right image). AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: . Top panel: schematic representation of a 5 dpf larva (reproduced with modifications from [16]); red line = aorta; blue line = vein. The larva used expresses eGFP under the control of the vascular kdrl promotor [ Tg(kdrl: eGFP) fish line]. Bottom panels: spinning disk confocal images obtained either in the trunk region (left: AGM) or in the tail region (right: CHT). Images shown were obtained either from maximal z-projections of z-stacks or from single z-sections with 1.5× magnification of regions in white boxes. Green channel: vascular cells expressing soluble eGFP (aortic and veinous endothelial cells in the trunk and tail regions). Magenta: RNAscope spots indicating expression of cmyb mRNAs in HSPCs. Note that RNAscope signals cannot be superposed to individual cells because HSPCs are no longer expressing eGFP driven by the vascular kdrl promoter, as is the case in 48 hpf embryos (owing to distant timing from their emergence, half-life of eGFP, and division cycles diluting the fluorescent protein). Note the structural evolution of the vein niche, notably in the CHT, in comparison with the 48 hpf embryo shown in Figure 3, and the relatively regular positioning of hematopoietic clusters (delimited by dashed lines in the bottom right image). AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Control, Expressing, RNAscope, Comparison

    Top images show Ibidi dishes, with the recommended dissection area on the top and the agarose mounting area at the bottom (white rectangles). 48 hpf embryos (a, b) and 5 dpf larvae (a’, b’) were either (a, a’) or not (b, b’) treated with proteinase K and all of them with RNAscope reagents. Images show that the whole procedure leads to virtually total transparency of the specimen, which reaches a maximum for embryos [becoming barely visible as in (a)]. This transparency requires controlling all steps of reagent/buffer removal under the binoculars. Yolks were dissected, as well as the eyes facing the glass bottom of the dish to ensure flatness.

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: Top images show Ibidi dishes, with the recommended dissection area on the top and the agarose mounting area at the bottom (white rectangles). 48 hpf embryos (a, b) and 5 dpf larvae (a’, b’) were either (a, a’) or not (b, b’) treated with proteinase K and all of them with RNAscope reagents. Images show that the whole procedure leads to virtually total transparency of the specimen, which reaches a maximum for embryos [becoming barely visible as in (a)]. This transparency requires controlling all steps of reagent/buffer removal under the binoculars. Yolks were dissected, as well as the eyes facing the glass bottom of the dish to ensure flatness.

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Dissection, RNAscope

    All data plotted are extracted from a single caudal hematopoietic tissue (CHT) z-stack showing the expression of cmyb (RNAscope spots) in hematopoietic cells expressing eGFP under the control of the runx1+23 enhancer. (A) Imaris Vantage 2D plotting, cell number of RNAscope spots relative to the cell area (µm). (B) Imaris vantage cumulative distribution function (CDF) plotting, cumulative number of spots percentage relative to the shortest distance to cell surface (µm). Solid pink line: our data; dashed pink line and light pink interval: confidence interval’s random distribution; red vertical line: calculated attraction distance. (C) Data extraction from Imaris and plotting in R studio using the ggstatsplot package. Top plot shows the correlation between cell size (µm) and number of spots per cell. Bottom plot shows the number of spots per cell depending on cell size [large cell > 11,490 voxels (corresponding to the median cell size), small cells < 11,490 voxels]. The code to generate the plots is given in Data analysis, section C.

    Journal: Bio-protocol

    Article Title: Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

    doi: 10.21769/BioProtoc.5269

    Figure Lengend Snippet: All data plotted are extracted from a single caudal hematopoietic tissue (CHT) z-stack showing the expression of cmyb (RNAscope spots) in hematopoietic cells expressing eGFP under the control of the runx1+23 enhancer. (A) Imaris Vantage 2D plotting, cell number of RNAscope spots relative to the cell area (µm). (B) Imaris vantage cumulative distribution function (CDF) plotting, cumulative number of spots percentage relative to the shortest distance to cell surface (µm). Solid pink line: our data; dashed pink line and light pink interval: confidence interval’s random distribution; red vertical line: calculated attraction distance. (C) Data extraction from Imaris and plotting in R studio using the ggstatsplot package. Top plot shows the correlation between cell size (µm) and number of spots per cell. Bottom plot shows the number of spots per cell depending on cell size [large cell > 11,490 voxels (corresponding to the median cell size), small cells < 11,490 voxels]. The code to generate the plots is given in Data analysis, section C.

    Article Snippet: RNAscope TM negative control probe DapB (of Bacillus subtilis strain) (ACD BioTechne, catalog numbers: 310043, 310043-C2, 310043-C3 for the three channels C1, C2, and C3, respectively) 13.

    Techniques: Expressing, RNAscope, Control, Extraction