negative control shrna nc shrna  (Genechem)

Journal: Bioscience Reports

Article Title: G3BP1 is a SWI/SNF-bound regulator of transcription that modulates activation of STATs

doi: 10.1042/BSR20250290

Figure Lengend Snippet: ( A, B ) G3BP1 was transiently silenced with siRNA, or not; cells were then serum-starved and stimulated with PDGF-BB, as indicated. Activation of PDGFR, STAT1, ERK1/2, AKT, PLCγ, and STAT3 signaling pathways was determined by immunoblotting for phosphorylated and total amounts of the signaling proteins. The experiments were repeated four times.

Article Snippet: AG01523 cells were transiently transfected with 20 nM siRNA of Trilencer-27 G3BP siRNA (#SR, OriGene Technologies, U.S.A.) or 20 nM scrambled negative control siRNA (#SR30004, OriGene Technologies, U.S.A.), using SilentFect reagent (Bio-Rad), and incubated for 72 to 96 h at 37°C in a CO 2 incubator.

Techniques: Activation Assay, Protein-Protein interactions, Western Blot

Journal: Bioscience Reports

Article Title: G3BP1 is a SWI/SNF-bound regulator of transcription that modulates activation of STATs

doi: 10.1042/BSR20250290

Figure Lengend Snippet: (A–F) After transient silencing of G3BP1 with siRNA, or not, and serum starvation overnight, AG01523 cells were stimulated with PDGF-BB for 1 h (black bars) or left unstimulated (0 h, gray bars). mRNA expression of G3BP1 ( A ), STAT3 ( B ), STAT1 ( C ), FOS ( D ), MYC ( E ), and CCND1 (cyclin D1) ( F ) is presented relative to the expression of control gene HPRT . Statistical analysis was performed on four independent repeats using Student’s t -test. *, P- value <0.05; **, P <0.01. The experiments were repeated six times.

Article Snippet: AG01523 cells were transiently transfected with 20 nM siRNA of Trilencer-27 G3BP siRNA (#SR, OriGene Technologies, U.S.A.) or 20 nM scrambled negative control siRNA (#SR30004, OriGene Technologies, U.S.A.), using SilentFect reagent (Bio-Rad), and incubated for 72 to 96 h at 37°C in a CO 2 incubator.

Techniques: Expressing, Control

Journal: Bioscience Reports

Article Title: G3BP1 is a SWI/SNF-bound regulator of transcription that modulates activation of STATs

doi: 10.1042/BSR20250290

Figure Lengend Snippet: ( A ) G3BP1 was knocked down by siRNA, or not, in AG01523 fibroblasts. Cells were then grown in medium containing 1% FBS and increasing concentrations of PDGF-BB. The amount of DNA and, therefore, the proliferation rate was determined by measuring absorption of fluorescently labeled DNA-intercalating dye using the CyQuant assay. The experiments were repeated three times. ( B ) Schematic illustration of the findings of the study. G3BP1 acts as a co-activator of PDGF-BB-induced activation of STAT3 and as a co-repressor of the transcription of cyclin D1 and STAT1 due to its association with the SWI/SNF chromatin remodeling complex.

Article Snippet: AG01523 cells were transiently transfected with 20 nM siRNA of Trilencer-27 G3BP siRNA (#SR, OriGene Technologies, U.S.A.) or 20 nM scrambled negative control siRNA (#SR30004, OriGene Technologies, U.S.A.), using SilentFect reagent (Bio-Rad), and incubated for 72 to 96 h at 37°C in a CO 2 incubator.

Techniques: Labeling, CyQUANT Assay, Activation Assay