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cell lines nccit  (ATCC)


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    ATCC cell lines nccit
    Cell Lines Nccit, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nccit+cell+lines/NCCIT/pmc12677466-268-0-3
    Average 95 stars, based on 262 article reviews
    cell lines nccit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Modification:

    Article Title: KPT6566 induces apoptotic cell death and suppresses the tumorigenicity of testicular germ cell tumors
    Article Snippet: .. P19 and NCCIT cell lines were procured from the American Type Culture Collection (ATCC) and cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM; ThermoFisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Sigma-Aldrich), GlutaMAX (ThermoFisher Scientific), and 1% Penicillin-Streptomycin (ThermoFisher Scientific). .. Cells were maintained in a humidified 37°C incubator (ThermoFisher Scientific) with 5% CO 2 .

    Multiple Displacement Amplification:

    Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer
    Article Snippet: All cultures were routinely screened for the absence of mycoplasma contamination using the ATCC Universal Mycoplasma Detection Kit (Manassas, VA). .. PC-3, VCAP, and NCCIT cell lines were purchased from ATCC, and the Du145, LNCaP, LAPC-4, C4-2, CWR22Rv1, CWR-R1, MDA-PCa2B, and E006AA cell lines were generously provided by Dr. John Isaacs at The Johns Hopkins University and have been previously characterized . .. The human embryonic stem cell line WA01(H1) was acquired from WiCell (Madison, WI) and cultured using the feeder-independent protocol using mTeSR1 media (Stem Cell Technologies; Vancouver, B.C.).



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    a The relationship between stemness-associated genes and pseudotime. b Microscopic images of <t>NCCIT</t> spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images <t>of</t> <t>Tcam-2</t> spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.
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    a The relationship between stemness-associated genes and pseudotime. b Microscopic images of <t>NCCIT</t> spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images <t>of</t> <t>Tcam-2</t> spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.
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    a The relationship between stemness-associated genes and pseudotime. b Microscopic images of <t>NCCIT</t> spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images <t>of</t> <t>Tcam-2</t> spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.
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    Image Search Results


    a The relationship between stemness-associated genes and pseudotime. b Microscopic images of NCCIT spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images of Tcam-2 spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.

    Journal: Cell Death Discovery

    Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

    doi: 10.1038/s41420-025-02802-4

    Figure Lengend Snippet: a The relationship between stemness-associated genes and pseudotime. b Microscopic images of NCCIT spheres after DPPA4 knockdown (scale bars: 100 µm (left); 50 µm (right)). Violin plot showing reduced sphere size and bar plot indicating decreased expression of stemness-related genes following DPPA4 knockdown. c Microscopic images of Tcam-2 spheres after DPPA4 knockdown. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after DPPA4 knockdown. d Microscopic images of NCCIT spheres after PSMA7 knockdown. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PSMA7 knockdown. e Microscopic images of Tcam-2 spheres after PSMA7 knockdown. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes after PSMA7 knockdown. f Microscopic images of NCCIT spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot indicating reduced stemness-related gene expression following PAGE5 overexpression. g Microscopic images of Tcam-2 spheres after PAGE5 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after PAGE5 overexpression. h Microscopic images of NCCIT spheres after SAT1 overexpression. Violin plot showing reduced sphere size and bar plot showing decreased expression of stemness-related genes following SAT1 overexpression. i Microscopic images of Tcam-2 spheres after SAT1 overexpression. Violin plot showing smaller spheres and bar plot demonstrating reduced stemness-related gene expression after SAT1 overexpression.

    Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

    Techniques: Knockdown, Expressing, Gene Expression, Over Expression

    a Confluence rate of Tcam-2 and NCCIT cells in wound healing assays (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.05, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.05; NCCIT: Ctrl vs DPPA4 -KD: P < 0.001, Ctrl vs PSMA7 -KD: P < 0.001, Ctrl vs PAGE5 -OE: P < 0.001, Ctrl vs SAT1 -OE: P < 0.05). b Box plot showing proliferation scores across different cell types. c GSEA plot showing enrichment of MYC target v1 gene signature in embryonal carcinoma cells compared to seminoma cells. d GSEA plot showing enrichment of MYC target v2 gene signature in embryonal carcinoma cells compared to seminoma cells. e GSEA plot showing enrichment of mTORC1 signaling gene signature in embryonal carcinoma cells compared to seminoma cells. f GSEA plot showing enrichment of MYC target v1 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. g GSEA plot showing enrichment of MYC target v2 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. h GSEA plot showing enrichment of mTORC1 signaling gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. i Growth curves of Tcam-2 and NCCIT cells (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.0001, Ctrl vs SAT1 -OE: P < 0.0001; NCCIT: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.01).

    Journal: Cell Death Discovery

    Article Title: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis

    doi: 10.1038/s41420-025-02802-4

    Figure Lengend Snippet: a Confluence rate of Tcam-2 and NCCIT cells in wound healing assays (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.05, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.05; NCCIT: Ctrl vs DPPA4 -KD: P < 0.001, Ctrl vs PSMA7 -KD: P < 0.001, Ctrl vs PAGE5 -OE: P < 0.001, Ctrl vs SAT1 -OE: P < 0.05). b Box plot showing proliferation scores across different cell types. c GSEA plot showing enrichment of MYC target v1 gene signature in embryonal carcinoma cells compared to seminoma cells. d GSEA plot showing enrichment of MYC target v2 gene signature in embryonal carcinoma cells compared to seminoma cells. e GSEA plot showing enrichment of mTORC1 signaling gene signature in embryonal carcinoma cells compared to seminoma cells. f GSEA plot showing enrichment of MYC target v1 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. g GSEA plot showing enrichment of MYC target v2 gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. h GSEA plot showing enrichment of mTORC1 signaling gene signature in metastatic seminoma cells compared to non-metastatic seminoma cells. i Growth curves of Tcam-2 and NCCIT cells (Tcam-2: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.0001, Ctrl vs SAT1 -OE: P < 0.0001; NCCIT: Ctrl vs DPPA4 -KD: P < 0.01, Ctrl vs PSMA7 -KD: P < 0.01, Ctrl vs PAGE5 -OE: P < 0.01, Ctrl vs SAT1 -OE: P < 0.01).

    Article Snippet: The human embryonal carcinoma cell line NCCIT (ATCC, CRL-2073) and the seminoma cell line Tcam-2 (Otwo, Wuhan, China) were used in this study.

    Techniques: