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In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of <t>CD56</t> and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.
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In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of CD56 and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.

Journal: Journal of Advanced Research

Article Title: Ion interference induced by Ca-Mn nanoplatform enhances ferroptosis and promotes immune response for osteosarcoma treatment

doi: 10.1016/j.jare.2025.06.022

Figure Lengend Snippet: In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of CD56 and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.

Article Snippet: The primary antibodies used were CD16 (#DF7007, Affinity), CD56 (60238–1-Ig, Proteintech), and CD8 (#AF5126, Affinity).

Techniques: In Vivo, Staining, Fluorescence, Immunofluorescence, Immunohistochemical staining

In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of CD56 and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.

Journal: Journal of Advanced Research

Article Title: Ion interference induced by Ca-Mn nanoplatform enhances ferroptosis and promotes immune response for osteosarcoma treatment

doi: 10.1016/j.jare.2025.06.022

Figure Lengend Snippet: In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of CD56 and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.

Article Snippet: The primary antibodies used were CD16 (#DF7007, Affinity), CD56 (60238–1-Ig, Proteintech), and CD8 (#AF5126, Affinity).

Techniques: In Vivo, Staining, Fluorescence, Immunofluorescence, Immunohistochemical staining

In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of CD56 and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.

Journal: Journal of Advanced Research

Article Title: Ion interference induced by Ca-Mn nanoplatform enhances ferroptosis and promotes immune response for osteosarcoma treatment

doi: 10.1016/j.jare.2025.06.022

Figure Lengend Snippet: In vivo evaluation properties of HMnO 2 @CaO 2 in K7M2-tumor-bearing mice. (a) Schematic representation of a tumor metastasis model and corresponding treatment strategy. (b) Images of primary tumors from mice following a 21-day treatment period. (c) Tumor volume curves after different treatments. (d) Body weight changes after 21-day treatment period. (e) H&E and Ki67 (red) stained after different treatments, scale bar = 100 μm. (f) Corresponding fluorescence intensity of GRP78 and (g) GPX4. (h) Immunofluorescence staining results of GRP78 and GPX4, scale bar = 50 μm. (i) Immunofluorescence staining results of iNOS (red), and immunohistochemical staining results of Arg-1 (yellow), scale bar = 100 μm. (j) The image of CD8 + T cells in the tumor after various treatment, scale bar = 100 nm. (k) The level of CD8 + cells in different treatments. The cytokines secretion of (l) IFN-γ and (m) IL-6 in serum after different treatments. (n) The CLSM of CD56 and CD16, scale bar = 100 nm. (o) The level of NK cells after different treatments.

Article Snippet: The primary antibodies used were CD16 (#DF7007, Affinity), CD56 (60238–1-Ig, Proteintech), and CD8 (#AF5126, Affinity).

Techniques: In Vivo, Staining, Fluorescence, Immunofluorescence, Immunohistochemical staining