Journal: bioRxiv

Article Title: LSD1 inhibitors induce neuronal differentiation of Merkel cell carcinoma by disrupting the LSD1-CoREST complex and activating TGFβ signaling

doi: 10.1101/2020.04.14.041657

Figure Lengend Snippet: a , Bulk mouse skin labeled with IgM-PE mouse isotype control. b , Bulk mouse skin labeled with NCAM-PE. DAPI - cells were sorted. c , Merkel cells labeled with IgM-PE mouse isotype control. d , Merkel cells labeled with NCAM-PE. DAPI - /NCAM + cells were sorted. Each plot shows the subpopulation gated for in the preceding plot to the left.

Article Snippet: Merkel cells were stained with anti-PSA-NCAM-PE antibody (Miltenyi Biotec, Cat. 130-117-394) or with mouse IgM-PE isotype control (Miltenyi Biotec, Cat. 130-120-070) according to manufacturer’s instructions, for 10 min at 4°C.

Techniques: Labeling, Control

Journal: Cell Death & Disease

Article Title: The histone methyltransferase DOT1L inhibits osteoclastogenesis and protects against osteoporosis

doi: 10.1038/s41419-017-0040-5

Figure Lengend Snippet: Proteins previously reported to be associated with OC differentiation (indicated by the large circle) and their first connected nodes (indicated by the small circle) were extracted from Fig. . The width of the lines connecting the proteins indicates the connection score obtained from the database. Up- or downregulation is indicated by the color of nodes (red, upregulated; green, downregulated). a–c Network of OC-associated DEPs identified in 40-h pre-OCs, 60-h pre-OCs, and 60-h OCs. Function groups were classified on the basis of UniProt function and published reports. Panels a – c share one color ruler. d Verification of upregulation of protein expression by western blotting. Upregulation of CD9 in 60-h pre-OCs and MMP9 in 60-h OCs after DOT1L inhibition was detected in proteomic data and verified by western blotting using specific antibodies. DEPs: differentially expressed proteins

Article Snippet: Western blot analysis was performed using the following Abs: DOT1L (ab157199), TRAP (ab191406), CTSK (ab19027), MMP9 (ab137867), CD9 (ab92726), GAPDH (M171-3), H3K79me2 (ab3594), H3K79me1 (ab2886), H3K9me3 (ab8898), H3K9me2 (ab1220), asymmetric H3R17me2 (ab8284), Pan H3 (ab1791), H4K20me3 (ab9053), H4K20me2 (ab9052), symmetric H4R3me2 (ab5823), and Pan H4 (ab10158) (all purchased from Abcam (Cambridgeshire, UK). p-p65 (3033), p65 (8242), H3K36me3 (9763), H3K36me2 (2901), H3K27me3 (9733), H3K27me2 (9728), and H3K4me3 (9751) were purchased from Cell Signaling Technology (MA, USA).

Techniques: Expressing, Western Blot, Inhibition

Journal: iScience

Article Title: The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis.

doi: 10.1016/j.isci.2025.112608

Figure Lengend Snippet: Figure 8. Expressions of NCAM1 and SEMA5A in CS (A) Immunohistochemistry (IHC) staining of NCAM1 and SEMA5A in the intervertebral disc (NCAM1, nucleus pulposus; SEMA5A, cartilage endplate) of CS, LS, and NC. (B) IHC score (IOD/area) of NCAM1 and SEMA5A in 24 CS, 7 LS, and 5 NC patients. (C) Immunofluorescence of NCAM1 and notochord markers in nucleus pulposus, and SEMA5A and pericyte markers in cartilage endplate of CS. The scale bars in panel A indicated 500 μm, and the scale bars in panel C indicated 50 μm. Kruskal-Wallis test was used to compare the data in CS, LS, and NC, followed by Dunn’s multiple comparison test in every two groups for post-hoc analysis. ns, not significant; **p < 0.01; ****p < 0.0001. Abbreviations: CS, congenital scolosis; LS, lumbar spondylolysis; NC, normal control.

Article Snippet: After antigen blocking, the sections were incubated overnight at 4◦C with the following primary antibodies: CCN1 (Cell Signaling Technology, 39382), CCN2 (Abcam, ab6992), ECRG4 (Abcam, ab224077), DBP (Thermo Fisher Scientific, PA540501), DLK1 (Proteintech, 10636-1-AP), EPYC (Abcam, ab122449), HOXC6 (Abcam, ab41587), NCAM1 (Cell Signaling Technology, 3576S), SEMA5A (R&D, AF5896).

Techniques: Immunohistochemistry, Immunofluorescence, Comparison, Control