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nbp2 44607  (Novus Biologicals)


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    Novus Biologicals nbp2 44607
    Nbp2 44607, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nbp2+44607/WT1+Antibody+(WT1%2F857+%2B+6F-H2)/pmc11814332__41467_2025_56891_MOESM1_ESM-118-247-245
    Average 93 stars, based on 8 article reviews
    nbp2 44607 - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism
    Article Snippet: .. Slides were blocked using 5% bovine serum albumin for 30 minutes and then incubated overnight at 4 °C with both FGF1 antibody (Abcam, ab207321, 1:200) and an antibody of a specific marker (anti-smooth muscle actin, Abcam, ab7817, 1:200; anti-Wilms tumor 1 [Novus Biologicals, Littleton, CO], NBP2-44607, 1:200); and anti-aquaporin 1 [Santa Cruz Biotechnology, Inc., Dallas, TX, sc-32737, 1:200; anti-CD68 from Abcam, ab955, 1:200), respectively. .. Slides were then correspondingly incubated with 2 kinds of secondary antibody (TRITC-labeled antibody from Abcam, ab6786, 1:500; Alexa Fluor–labeled antibody from Abcam, ab150075, 1:500) at 37 °C for 1 hour, and washed by PBS for 4 times.

    Marker:

    Article Title: Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism
    Article Snippet: .. Slides were blocked using 5% bovine serum albumin for 30 minutes and then incubated overnight at 4 °C with both FGF1 antibody (Abcam, ab207321, 1:200) and an antibody of a specific marker (anti-smooth muscle actin, Abcam, ab7817, 1:200; anti-Wilms tumor 1 [Novus Biologicals, Littleton, CO], NBP2-44607, 1:200); and anti-aquaporin 1 [Santa Cruz Biotechnology, Inc., Dallas, TX, sc-32737, 1:200; anti-CD68 from Abcam, ab955, 1:200), respectively. .. Slides were then correspondingly incubated with 2 kinds of secondary antibody (TRITC-labeled antibody from Abcam, ab6786, 1:500; Alexa Fluor–labeled antibody from Abcam, ab150075, 1:500) at 37 °C for 1 hour, and washed by PBS for 4 times.

    other:

    Article Title: CerS6 links ceramide metabolism to innate immune responses in diabetic kidney disease
    Article Snippet: Antibody Source Catalog number Application Rabbit anti- CerS6 Gentex Cat# GTX17028 dilution 1:1000 for WB (mouse kidney tissue) Rabbit anti- CerS6 Novus Cat#76965 dilution 1:500 for IHC (mouse kidney section), 1:500 for IF (mouse kidney section) Rabbit anti- CerS6 Sigma Cat# HPA063527 dilution 1:1000 for IF (human kidney section), 1:1000 for WB (HPC) Rabbit anti- STING CST Cat# 13647S dilution 1:1000 for WB, 1:200 for IF Rabbit anti- cGAS CST Cat# 15102T dilution 1:1000 for WB (HPC) Rabbit anti- cGAS Proteintech Cat#68640-1-Ig dilution 1:2000 for WB (mouse kidney tissue), 1:500 for IF (mouse kidney section) Rabbit anti- cGAS Abcam Cat# ab302617 dilution 1:50 for IF (HPC coverslip) Rabbit anti-p- TBK1 CST Cat# 5483S dilution 1:1000 for WB Rabbit anti- TBK1 Abcam Cat# ab40676 dilution 1:5000 for WB Rabbit anti-p- NF-κB (p-p65) CST Cat# 3033S dilution 1:1000 for WB Rabbit anti-NF- κB (p65) Santa Cruz Cat# sc-8008 dilution 1:500 for WB Rabbit anti- VDAC1 Abcam Cat# ab306581 dilution 1:1000 for WB, 1:1000 Mouse anti- BAX Proteintech Cat# 60267-1-Ig dilution 1:10000 for WB Mouse anti- dsDNA Abcam Cat# ab27156 dilution 1:500 for IF Mouse anti- Tomm20 Novus Cat# H00009804 dilution 1:200 for IF Rabbit anti- CD68 Abcam Cat# ab303565 dilution 1:100 for IF Rabbit anti- Nephrin Novus Cat# NBP1-30130 dilution 1:200 for IF Rabbit anti- Nephrin Proteintech Cat# 22912-1-AP dilution 1:2000 for WB Mouse anti- Synaptopodin Progen Cat# 65194 dilution 1:100 for IF Mouse anti- Synaptopodin Santa Cruz Cat# sc-515842 dilution 1:500 for WB Mouse anti-WT- 1 Novus Cat# NBP2-44607 dilution 1:200 for IF Rabbit anti-WT- 1 BOSTER Cat# BM4216 dilution 1:1000 for WB Mouse anti-α- tubulin Proteintech Cat# 66031-1-Ig dilution 1:20000 for WB Mouse anti-β- actin Proteintech Cat# 66009-1-Ig dilution 1:20000 for WB Mouse anti- GAPDH Proteintech Cat# 60004-1-Ig dilution 1:50000 for WB



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    Fig. 2. Diosgenin regulated SIRT6 and reduced lipid accumulation in glomerulus. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus (B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and <t>WT1</t> (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus(D, n = 6 for each group); (E) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).
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    Antibodies and assay kits

    Journal: Kidney360

    Article Title: AMPK-SP1–Guided Dynein Expression Represents a New Energy-Responsive Mechanism and Therapeutic Target for Diabetic Nephropathy

    doi: 10.34067/KID.0000000000000392

    Figure Lengend Snippet: Antibodies and assay kits

    Article Snippet: Mouse anti-WT1 , Novus Biologicals , NBP2-44607.

    Techniques:

    Fig. 2. Diosgenin regulated SIRT6 and reduced lipid accumulation in glomerulus. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus (B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus(D, n = 6 for each group); (E) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6.

    doi: 10.1016/j.phymed.2022.154276

    Figure Lengend Snippet: Fig. 2. Diosgenin regulated SIRT6 and reduced lipid accumulation in glomerulus. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus (B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus(D, n = 6 for each group); (E) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Article Snippet: The primary antibody includes SIRT6 (ab191385, abcam), WT1 (NBP2-44607,NOVUS), NEPHRIN Z. Wang et al. Phytomedicine 104 (2022) 154276 (ab216341, abcam), PODOCIN (ab181143, abcam), DESMIN (A3736, ABclonal).

    Techniques: Staining, Immunohistochemistry, Labeling, Immunofluorescence, Marker, Fluorescence, Quantitative RT-PCR

    Fig. 4. The effect of diosgenin in the regulation of SIRT6 and reduction of lipid accumulation in glomerulus was blocked by by OSS_128167 and MDL800 had the same effect as diosgenin. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus(B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus (D, n = 6 for each group); (E) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6.

    doi: 10.1016/j.phymed.2022.154276

    Figure Lengend Snippet: Fig. 4. The effect of diosgenin in the regulation of SIRT6 and reduction of lipid accumulation in glomerulus was blocked by by OSS_128167 and MDL800 had the same effect as diosgenin. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus(B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus (D, n = 6 for each group); (E) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Article Snippet: The primary antibody includes SIRT6 (ab191385, abcam), WT1 (NBP2-44607,NOVUS), NEPHRIN Z. Wang et al. Phytomedicine 104 (2022) 154276 (ab216341, abcam), PODOCIN (ab181143, abcam), DESMIN (A3736, ABclonal).

    Techniques: Staining, Immunohistochemistry, Labeling, Immunofluorescence, Marker, Fluorescence, Quantitative RT-PCR

    Fig. 5. Diosgenin protected against MPC5 injury by regulating SIRT6. (A-E) Protein including NEPHRIN and PODOCIN measured by western blot (A) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (B) and NEPHRIN quantification (C, n = 6 for each group), PODOCIN quantification (D,n = 6 for each group) and DESMIN quantification (E,n = 6 for each group) by Image Pro Plus; (F-J) Protein including NEPHRIN and PODOCIN measured by western blot (F) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (G) and NEPHRIN quantification (H, n = 6 for each group),PODOCIN quantification (I, n = 6 for each group) and DESMIN quantification (J, n = 6 for each group) by Image Pro Plus; (K and L) Double labeling of immunofluorescence about SIRT6 and WT1 by confocal,scale bar, 50 μm (K) and SIRT6 quantification by Image Pro Plus (L, n = 6 for each group).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6.

    doi: 10.1016/j.phymed.2022.154276

    Figure Lengend Snippet: Fig. 5. Diosgenin protected against MPC5 injury by regulating SIRT6. (A-E) Protein including NEPHRIN and PODOCIN measured by western blot (A) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (B) and NEPHRIN quantification (C, n = 6 for each group), PODOCIN quantification (D,n = 6 for each group) and DESMIN quantification (E,n = 6 for each group) by Image Pro Plus; (F-J) Protein including NEPHRIN and PODOCIN measured by western blot (F) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (G) and NEPHRIN quantification (H, n = 6 for each group),PODOCIN quantification (I, n = 6 for each group) and DESMIN quantification (J, n = 6 for each group) by Image Pro Plus; (K and L) Double labeling of immunofluorescence about SIRT6 and WT1 by confocal,scale bar, 50 μm (K) and SIRT6 quantification by Image Pro Plus (L, n = 6 for each group).

    Article Snippet: The primary antibody includes SIRT6 (ab191385, abcam), WT1 (NBP2-44607,NOVUS), NEPHRIN Z. Wang et al. Phytomedicine 104 (2022) 154276 (ab216341, abcam), PODOCIN (ab181143, abcam), DESMIN (A3736, ABclonal).

    Techniques: Western Blot, Immunofluorescence, Labeling