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native polyacrylamide gel  (Bio-Rad)


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    Bio-Rad native polyacrylamide gel
    Native Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/native+page+gels/Native+PAGE+Sample+Buffer/pmc13128918-379-43-59
    Average 95 stars, based on 148 article reviews
    native polyacrylamide gel - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Clear Native PAGE:

    Article Title: JA-responsive R2R3-type MYB transcription factor OsMYB4P confers broad-spectrum antiviral immunity in rice.
    Article Snippet: For EMSA, probes were incubated with recombinant proteins in the indicated combinations (as shown in Figures 4c and 7a), at 25 °C in a reaction system prepared according to the manufacturer’s instructions for the LightShiftTM EMSA Optimization & Control Kit (Thermo Fisher Scientific). .. The free and bound probes were separated on 4–6% native PAGE gels (59 TBE, 30% Acr-Bis, 40% glycerinum, 10% APS, TEMED and ddH2O), and the shifted signals were visualized using the Chemiluminescent Nucleic Acid Detection Module with the BIORAD ChemiDoc MP Imaging System. ..

    Article Title: Novel palm shortening substitute using a combination of rapeseed oil, linseed meal and beta-glucan.
    Article Snippet: Twenty μl of sample or 30 μl of the Precision Plus protein standard (Bio-Rad Laboratories Ltd., UK) were loaded onto the wells. .. The gels were run for 1 h at 100 V in Tris/Glycine/SDS buffer for the SDS-PAGE gels (Bio-Rad Laboratories Ltd., UK) and in native buffer for the Native PAGE gels (Bio-Rad Laboratories Ltd., UK). ..

    Article Title: Ferritin microheterogeneity, subunit composition, functional, and physiological implications.
    Article Snippet: .. Native PAGE gels (7% acrylamide) stained with Coomassie blue (Sigma-Aldrich) and SDS − PAGE gels (12% acrylamide) with densitometry analysis (Bio-Rad system) were used to examine the integrity of the ferritin samples and to estimate the ferritin subunit composition from band intensities, respectively. ..

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Extraction of an emulsifying agent from the cellulose-based filtration aid of cold-pressed rapeseed oil
    Article Snippet: Twenty μl of sample or 30 μl of Precision Plus protein standard (Bio-Rad Laboratories, UK) were loaded onto the wells. .. The SDS-PAGE and Native PAGE gels were run for 1 h at 100 V in Tris-glycine-SDS buffer (Bio-Rad Laboratories, UK) and native buffer (Bio-Rad Laboratories, UK), respectively. ..

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.

    Article Title: JA ‐responsive R2R3 ‐type MYB transcription factor OsMYB4P confers broad‐spectrum antiviral immunity in rice
    Article Snippet: For EMSA, probes were incubated with recombinant proteins in the indicated combinations (as shown in Figures and ), at 25 °C in a reaction system prepared according to the manufacturer's instructions for the LightShiftTM EMSA Optimization & Control Kit (Thermo Fisher Scientific). .. The free and bound probes were separated on 4–6% native PAGE gels (5× TBE, 30% Acr‐Bis, 40% glycerinum, 10% APS, TEMED and ddH 2 O), and the shifted signals were visualized using the Chemiluminescent Nucleic Acid Detection Module with the BIO‐RAD ChemiDoc MP Imaging System. ..

    Article Title: A whole-animal phenotypic drug screen identifies suppressors of atherogenic lipoproteins
    Article Snippet: .. Native-PAGE gels (3% acrylamide:bis-solution 19:1 (BioRad, 1610144)/0.08% ammonium persulfate/0.006% tetramethylethylenediamine (TEMED)/1X TBE) were prepared for individual experiments. .. Gels were assembled into mini-protean electrophoresis rigs (BioRad) at 4 °C, filled with pre-chilled 1X TBE, and pre-run at 50V for 30 minutes to equilibrate the gel before sample addition.

    Imaging:

    Article Title: JA-responsive R2R3-type MYB transcription factor OsMYB4P confers broad-spectrum antiviral immunity in rice.
    Article Snippet: For EMSA, probes were incubated with recombinant proteins in the indicated combinations (as shown in Figures 4c and 7a), at 25 °C in a reaction system prepared according to the manufacturer’s instructions for the LightShiftTM EMSA Optimization & Control Kit (Thermo Fisher Scientific). .. The free and bound probes were separated on 4–6% native PAGE gels (59 TBE, 30% Acr-Bis, 40% glycerinum, 10% APS, TEMED and ddH2O), and the shifted signals were visualized using the Chemiluminescent Nucleic Acid Detection Module with the BIORAD ChemiDoc MP Imaging System. ..

    Article Title: JA ‐responsive R2R3 ‐type MYB transcription factor OsMYB4P confers broad‐spectrum antiviral immunity in rice
    Article Snippet: For EMSA, probes were incubated with recombinant proteins in the indicated combinations (as shown in Figures and ), at 25 °C in a reaction system prepared according to the manufacturer's instructions for the LightShiftTM EMSA Optimization & Control Kit (Thermo Fisher Scientific). .. The free and bound probes were separated on 4–6% native PAGE gels (5× TBE, 30% Acr‐Bis, 40% glycerinum, 10% APS, TEMED and ddH 2 O), and the shifted signals were visualized using the Chemiluminescent Nucleic Acid Detection Module with the BIO‐RAD ChemiDoc MP Imaging System. ..

    SDS Page:

    Article Title: Novel palm shortening substitute using a combination of rapeseed oil, linseed meal and beta-glucan.
    Article Snippet: Twenty μl of sample or 30 μl of the Precision Plus protein standard (Bio-Rad Laboratories Ltd., UK) were loaded onto the wells. .. The gels were run for 1 h at 100 V in Tris/Glycine/SDS buffer for the SDS-PAGE gels (Bio-Rad Laboratories Ltd., UK) and in native buffer for the Native PAGE gels (Bio-Rad Laboratories Ltd., UK). ..

    Article Title: Ferritin microheterogeneity, subunit composition, functional, and physiological implications.
    Article Snippet: .. Native PAGE gels (7% acrylamide) stained with Coomassie blue (Sigma-Aldrich) and SDS − PAGE gels (12% acrylamide) with densitometry analysis (Bio-Rad system) were used to examine the integrity of the ferritin samples and to estimate the ferritin subunit composition from band intensities, respectively. ..

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Extraction of an emulsifying agent from the cellulose-based filtration aid of cold-pressed rapeseed oil
    Article Snippet: Twenty μl of sample or 30 μl of Precision Plus protein standard (Bio-Rad Laboratories, UK) were loaded onto the wells. .. The SDS-PAGE and Native PAGE gels were run for 1 h at 100 V in Tris-glycine-SDS buffer (Bio-Rad Laboratories, UK) and native buffer (Bio-Rad Laboratories, UK), respectively. ..

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.

    Staining:

    Article Title: Ferritin microheterogeneity, subunit composition, functional, and physiological implications.
    Article Snippet: .. Native PAGE gels (7% acrylamide) stained with Coomassie blue (Sigma-Aldrich) and SDS − PAGE gels (12% acrylamide) with densitometry analysis (Bio-Rad system) were used to examine the integrity of the ferritin samples and to estimate the ferritin subunit composition from band intensities, respectively. ..

    Purification:

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.

    Blue Native PAGE:

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.

    Sample Prep:

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.

    Membrane:

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.

    Western Blot:

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit
    Article Snippet: .. The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad)., For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used., Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences)., , .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 10 6 ) into each well of a 12-well culture plate.

    Article Title: Pentameric M2e influenza vaccine candidate generates strong immunity with limited survival benefit.
    Article Snippet: .. SDS-PAGE, Blue native PAGE, and Western blotting The purity and oligomeric properties of the purified proteins were confirmed on SDS-PAGE and Blue native-PAGE (Mini-PROTEAN TGXTM, Bio-Rad).14,15 For Blue native-PAGE analysis using 4% to 15% Native-PAGE gels (Mini-PROTEAN TGXTM, Bio-Rad, Hercules, CA, USA), Native-PAGE sample preparation buffer (Invitrogen, Waltham, MA, USA), and Bis-Tris running buffer were used.14,15 Proteins were transferred from SDS-PAGE to a PVDF membrane for western blotting with primary antibodies (mouse polyclonal sera, 1:500; anti-M2e 14C2, 1:1000, Abcam), and HRP-conjugated anti-mouse secondary antibody (1:2000, Jackson) was used for detection with chemiluminescence reagents (luminol and peroxidases, G Biosciences).14,15,17 .. For the IFA, MDCK-L cells (FR-58 obtained from the American Type Culture Collection (ATCC), Manassas, VA, USA) were seeded (0.2 × 106) into each well of a 12-well culture plate.



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    Feasibility validation and condition optimization utilizing fluorescence. (A) Native-PAGE gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).

    Journal: ACS Omega

    Article Title: Instrument-Free, Low-Cost and Dual-System Detection for Norovirus Based on Self-Assembling DNA Circuitry

    doi: 10.1021/acsomega.6c00618

    Figure Lengend Snippet: Feasibility validation and condition optimization utilizing fluorescence. (A) Native-PAGE gel image for the CHA reaction steps. Lane 1–7: H1, H2, T, H1 + T, H2 + T, H1 + H2, H1 + H2 + T. (B) Fluorescence monitoring of the CHA reaction progress was performed at 37 °C with 60 cycles and 30 s per cycle. (C) S/N ratio and fluorescence value of experimental and control groups at various temperatures after 30 min of reaction. (D) S/N ratio and fluorescence value of experimental and control groups at various H1:H2 ratios after 30 min of reaction at 35 °C. Data with error bars are represented as means ± standard deviation (n = 3).

    Article Snippet: Native PAGE gel, DNA marker, and loading buffer were ordered from Sangon Biotech Co., Ltd. (Shanghai, China).

    Techniques: Biomarker Discovery, Fluorescence, Clear Native PAGE, Control, Standard Deviation