napi iib (ABclonal Biotechnology)
Structured Review
Napi Iib, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/napi+iib/SLC34A2+Rabbit+pAb/pm42705192-87-13-17
Average 94 stars, based on 5 article reviews
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Membrane:Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 . Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 (TBST) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1- Ig, Proteintech, Wuhan, China), Saline:Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 . Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 (TBST) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1- Ig, Proteintech, Wuhan, China), Incubation:Article Title: MAPK signaling pathway participates in the regulation of intestinal phosphorus and calcium absorption in broiler chickens via 1,25-dihydroxyvitamin D 3 . Article Snippet: .. The PVDF membrane was then blocked with 5% nonfat powdered milk in Tris-buffered saline supplemented with tween-20 (TBST) at 37°C for 1 h. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies against GAPDH (1:10,000, 60004-1- Ig, Proteintech, Wuhan, China), Article Title: 1,25-dihydroxyvitamin D 3 enhances the expression of phosphorus transporters via vitamin D receptor in ligated duodenal loops of Arbor Acres male broilers. Article Snippet: .. The membranes were washed with TBST, and then incubated with primary antibodies against VDR (Cat. No. ab109234, Abcam, 1:5,000), Article Title: Vitamin D receptor silencing impairs the 1,25-dihydroxyvitamin D 3 -stimulated phosphorus absorption by suppressing NaPi-IIb gene expression in primary duodenal epithelial cells of broilers. Article Snippet: Total protein (20 μg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a PVDF membrane (catalog number IPVH00001; Merck Millipore, USA) and blocked with 5% (w/v) bovine serum albumin. .. Membranes were then incubated overnight with VDR (catalog number ab109234; abcam, UK; 1:5,000), |

