human cxcl7 nap 2 duoset elisa kit  (R&D Systems)

Journal: bioRxiv

Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability

doi: 10.64898/2026.04.12.718002

Figure Lengend Snippet: Overview of the NaP-TRAP workflow and examples of use cases. ( A ) NaP-TRAP can be performed using either complex reporter libraries or individual reporters, delivered in vivo by zebrafish embryo microinjection or in vitro by mammalian cell transfection, followed by pulldown, RNA purification, and either sequencing- or qPCR-based readout. ( B ) Principle of NaP-TRAP: FLAG-tagged nascent peptides on actively translating ribosomes are immunocaptured to enrich ribosome-associated reporter mRNAs (Pulldown). Reporter abundance is measured in the Input, and translation output is quantified as Pulldown/Input (NaP-TRAP TE). ( C ) Robust delivery and readout across a 100-fold range of injected reporter amounts in zebrafish embryos, with consistent NaP-TRAP TE across all doses. ( D ) Comparable reporter trends across mammalian cell types illustrated by differential translation of control versus oORF-containing reporters in HEK293T, H9, and MCF7 cells. ( E ) NaP-TRAP supports interrogation of diverse regulatory features across the reporter mRNA, including mRNA cap types, 5′-UTR elements (e.g., uORFs/oORFs), coding sequence codon optimality, 3′-UTR elements (e.g., miR-430 sites), and poly(A) tail length in zebrafish embryos. Cartoon diagrams were created individually in BioRender (Smith, J. (2025). BioRender.com/c248457 ).

Article Snippet: Cultured cell lines We have used NaP-TRAP on HEK293T (RRID: CVCL_0063, ATCC Cat. No. CRL-3216) but successfully performed it in various other cell lines including MCF7 (RRID: CVCL_0031, ATCC Cat. No. HTB-22) and H9 (RRID:CVCL_1240, ATCC Cat. No. HTB-176) .

Techniques: In Vivo, Microinjection, In Vitro, Transfection, Purification, Sequencing, Injection, Control

Journal: bioRxiv

Article Title: NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability

doi: 10.64898/2026.04.12.718002

Figure Lengend Snippet: Expected results from NaP-TRAP MPRA analysis pipeline. ( A ) Example histogram of per-insert read counts for one Input replicate with 7,839,828 reads. ( B ) Representative replicate-to-replicate correlations of NaP-TRAP translation efficiency (TE; Pulldown/Input) at 2 hpf and 6 hpf ( R values are Pearson correlations). ( C ) Hierarchical clustering heatmap of Pearson correlations showing TE similarity across replicates and conditions (zebrafish 2 hpf, zebrafish 6 hpf, and HEK293T). ( D ) Distribution of NaP-TRAP TE values at 2 hpf, with the top and bottom 10% of reporters highlighted as activated (blue) and repressed (orange), respectively. ( E ) Example k-mer enrichment analysis for activated and repressed reporter sets at 2 hpf. ( F ) Scatter plot comparing reporter NaP-TRAP TE at 2 versus 6 hpf, highlighting four reporter sets: reporters activated (blue) and repressed (orange) at both stages, reporters with higher TE at 2 hpf than at 6 hpf (2 hpf activated; green), and reporters with high TE at 6 hpf than at 2 hpf (6 hpf activated; pink). ( G ) k-mer enrichment analyses for each of the four reporter sets defined in (F): 2 hpf activated (green), 6 hpf activated (pink), globally repressed (orange) and globally activated (blue).

Article Snippet: Cultured cell lines We have used NaP-TRAP on HEK293T (RRID: CVCL_0063, ATCC Cat. No. CRL-3216) but successfully performed it in various other cell lines including MCF7 (RRID: CVCL_0031, ATCC Cat. No. HTB-22) and H9 (RRID:CVCL_1240, ATCC Cat. No. HTB-176) .

Techniques:

Journal: Journal of Neuroinflammation

Article Title: CXCL7/CXCR2 in the paraventricular thalamus mediates obesity-related pain

doi: 10.1186/s12974-025-03679-x

Figure Lengend Snippet: Increased inflammatory and oxidative stress levels in the PVT of DIO mice. A Quantification of CXCL7 in plasma from control or DIO mice. n = 3 mice per group, P = 0.0003. B and C Representative images ( B ) and summarized data ( C ) of the expression of CXCL7 in PVT Glu neurons from control and DIO mice. n = 5 mice per group, with one slice from each mouse. P < 0.0001. Scale bars, 10 μm. D - H Representative images ( D ) and quantification of IBA1 + cell numbers ( E ), intensity ( F ) and Imaris-based semiautomatic quantification of cell morphometry, including total branch length ( G ) and points ( H ) of IBA1 + -microglia from control and DIO mice. n = 5 mice per group for quantification of cell number and intensity, with one slice from each mouse. P (number) = 0.0081, P (intensity) = 0.0122. n= 6 mice for the control group and n = 7 mice for the DIO group in the quantification of branch length and points, with one slice from each mouse. P (length) = 0.014, P (intensity) = 0.0001. Scale bars, 10 μm (overview) and 3 μm (inset and rendering). I and J Representative images ( I ) and summarized data ( J ) of GPX4 in PVT Glu neurons from control and DIO mice. n = 6 mice per group, with one slice from each mouse. P = 0.0012. Scale bars, 10 μm. Statistical analysis was performed using Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Seven days later, a volume of 50 ng/μl CXCL7 protein (HY- P75698 , MCE, USA) dissolved in artificial cerebrospinal fluid (ACSF) was infused into the lateral ventricles (1 μl), or PVT (300 nl) was given once a day for three days in a row.

Techniques: Clinical Proteomics, Control, Expressing

Journal: Journal of Neuroinflammation

Article Title: CXCL7/CXCR2 in the paraventricular thalamus mediates obesity-related pain

doi: 10.1186/s12974-025-03679-x

Figure Lengend Snippet: Infusion of CXCL7 improved PVT Glu neuronal activity and induced pain hypersensitivity in control mice. A Schematic of the experimental procedure. B Image showing the site of the cannula implanted in the lateral ventricle of control mice. Scale bar, 500 μm. C and D Effects of infusion of CXCL7 in the lateral ventricle on the pain threshold in control mice treated with ACSF or CXCL7, using von Frey test ( C ) and Hargreaves tests ( D ). n = 5 mice per group, P = 0.0079 ( C ), P = 0.0007 ( D ). E Representative heatmaps of travel trajectory of control mice treated with ACSF or CXCL7 in the CPA test. F and G Summarized data of aversion index ( F ) and preference index ( G ) for ( E ), n = 7 mice per group. P < 0.0001 ( F and G ). ( H ) Image showing the site of the cannula implanted in the PVT of control mice. Scale bar, 500 μm. I and J Representative images ( I ) and summarized data ( J ) of CXCL7 on PVT Glu neurons in control mice treated with ACSF or CXCL7. n = 6 mice, with one slice from each mouse, P < 0.0001. Scale bar, 10 μm. K and L Representative images ( K ) and summarized data ( L ) of c-Fos in PVT from control mice treated with ACSF or CXCL7. n = 5 mice, with one slice from each mouse. P = 0.0061. Scale bar, 200 μm. M and N Sample traces ( M ) and summarized number of spikes ( N ) in PVT Glu neurons recorded from control mice treated with ACSF or CXCL7. n = 14 cells from 4 mice per group. P (time × column factor) = 0.0019, P (time) < 0.0001, P (column factor) = 0.001, F (1, 26) = 13.79. O and P Effects of the infusion of CXCL7 in PVT Glu neurons on the pain threshold using the von Frey test ( O ) and Hargreaves tests ( P ) of control mice treated with ACSF or CXCL7. n = 7 mice per group, P = 0.0002 ( O ), P = 0.0015 ( P ). Q Representative heatmaps of travel trajectory of control mice treated with ACSF or CXCL7 within PVT in the CPA test. ( R and S ) Summarized data of aversion index ( R ) and preference index ( S ) for ( Q ), n = 7 mice per group. P = 0.0003 ( R and S ). Statistical analysis was performed using Student’s t test in ( C ), ( D) , ( F) , ( G) , ( J) , ( L) , ( O) , ( P) , ( R) and ( S ), and two-way ANOVA followed by Bonferroni’s post hoc correction in ( N ). ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Seven days later, a volume of 50 ng/μl CXCL7 protein (HY- P75698 , MCE, USA) dissolved in artificial cerebrospinal fluid (ACSF) was infused into the lateral ventricles (1 μl), or PVT (300 nl) was given once a day for three days in a row.

Techniques: Activity Assay, Control

Journal: Journal of Neuroinflammation

Article Title: CXCL7/CXCR2 in the paraventricular thalamus mediates obesity-related pain

doi: 10.1186/s12974-025-03679-x

Figure Lengend Snippet: Mechanism of CXCL7-CXCR2 interaction in PVT Glu →BLA Glu neuronal circuit promotes the onset of obesity-related pain. In obese mice, elevated expression of the chemokine, CXCL7, results in the binding of CXCR2 receptors of PVT Glu neurons, thereby leading to increased activity in PVT Glu →BLA Glu neuronal circuit, which mediate obesity-related pain

Article Snippet: Seven days later, a volume of 50 ng/μl CXCL7 protein (HY- P75698 , MCE, USA) dissolved in artificial cerebrospinal fluid (ACSF) was infused into the lateral ventricles (1 μl), or PVT (300 nl) was given once a day for three days in a row.

Techniques: Expressing, Binding Assay, Activity Assay