Review



pt3 myr akt  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc pt3 myr akt
    Pt3 Myr Akt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pT3-myr-AKT-HA+(Plasmid+%2331789)/pm41928626-184-4-5
    Average 94 stars, based on 68 article reviews
    pt3 myr akt - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Localized heterochrony integrates overgrowth potential of oncogenic clones
    Article Snippet: Plasmid pminiTol2 ( Addgene #31829 ) was used as backbone vector and transgene cassettes ubi:eGFP , ubi:akt1 E18K , ubi:myr-p110 and hsp70:akt1 E18K were inserted into the multiple cloning site. .. Transgene cassettes were generated by inserting eGFP from pME-eGFP ( Tol2kit #383 ), murine myr-p110 from Myr-(iSH2-p85)-p110alpha-Myc ( Addgene #1410 ) or zebrafish akt1 E18K together with the SV40 late polyadenylation signal (SV40pA) downstream of the ubi or hsp70 promoter into pENTR5'_ubi ( Addgene #27320 ) or p5E-hsp70I ( Tol2kit #222 ), respectively. .. The full-length coding sequence of zebrafish akt1 was amplified from 24 h post fertilization cDNA and the E18K mutation was introduced using QuickChange Site-Directed mutagenesis (Agilent).

    Article Title: Localized heterochrony integrates overgrowth potential of oncogenic clones
    Article Snippet: The following plasmids were constructed for injection: pmTol2-ubi:eGFP (control construct), pmTol2-ubi:akt 1E18K -ubi:eGPF, pmTol2-ubi:myr-p110-ubi:eGPF. pminiTol2 (Addgene #31829) was used as backbone vector and transgene cassettes ( ubi:eGFP, ubi:akt1 E18K and ubi:myr-p110 ) were inserted into the multiple cloning site. .. Transgene cassettes were generated by inserting eGFP from pME-eGFP (Tol2kit #383), murine myr-p110 from Myr-(iSH2-p85)-p110alpha-Myc (Addgene #1410) or zebrafish akt1 E18K together with the SV40 late polyadenylation signal (SV40pA) downstream of the ubi promoter into pENTR5’ubi (Addgene #27320). .. The full length coding sequence of zebrafish akt1 was amplified from 24 hours post fertilization cDNA and the E18K mutation was introduced using QuickChange Site-Directed mutagenesis (Agilent).

    Article Title: Synapses in oligodendrocyte precursor cells are dynamic and contribute to Ca 2+ activity
    Article Snippet: .. We generated pME_ATG-PSD-95-GFP-TAA and p3E_p2A-PSD-95-GFP-TGA-polyA. pME_myrGFP and pME_myrTagRFP-T were generated using PCR and BP reaction, using Fyn myristoylation and palmitoylation domain. pGP-CMV-GCaMP6s (Addgene 40753) was used as a template and 3 primers were used in a Q5 PCR (15 cycles at 71° C and 20 cycles at 72° C) to amplify GCaMP6s and add Kozak sequence, myr (Fyn myristoylation and palmitoylation domain), attB1, and attB2 sequences. ..

    Isolation:

    Article Title: Color Processing in the Early Visual System of Drosophila.
    Article Snippet: .. For LexAop-twich-2c a NotI/XbaI fragment including Twitch-2C DNA was isolated from pUAST-Twich-2c (see above) and cloned into pJFRC19-13XLexAop2-IVS-myr::GFP (kindly provided by Gerald Rubin, Addgene plasmid #26224) by exchanging myr::GFP. rh3, rh4, promoter regions were amplified by standard PCR from pCaST-rh3/4-norpA plasmids (kindly provided by Chi-Hon Lee) adding 5‘-end AatII- and 3‘-end NgoMIV-restriction sites. .. The resulting AatII/NgoMIV fragment was cloned into pBPnLsLexA::GADfluw (kindly provided by Gerald Rubin, Addgene plasmid #26232).

    Clone Assay:

    Article Title: Color Processing in the Early Visual System of Drosophila.
    Article Snippet: .. For LexAop-twich-2c a NotI/XbaI fragment including Twitch-2C DNA was isolated from pUAST-Twich-2c (see above) and cloned into pJFRC19-13XLexAop2-IVS-myr::GFP (kindly provided by Gerald Rubin, Addgene plasmid #26224) by exchanging myr::GFP. rh3, rh4, promoter regions were amplified by standard PCR from pCaST-rh3/4-norpA plasmids (kindly provided by Chi-Hon Lee) adding 5‘-end AatII- and 3‘-end NgoMIV-restriction sites. .. The resulting AatII/NgoMIV fragment was cloned into pBPnLsLexA::GADfluw (kindly provided by Gerald Rubin, Addgene plasmid #26232).

    Plasmid Preparation:

    Article Title: Color Processing in the Early Visual System of Drosophila.
    Article Snippet: .. For LexAop-twich-2c a NotI/XbaI fragment including Twitch-2C DNA was isolated from pUAST-Twich-2c (see above) and cloned into pJFRC19-13XLexAop2-IVS-myr::GFP (kindly provided by Gerald Rubin, Addgene plasmid #26224) by exchanging myr::GFP. rh3, rh4, promoter regions were amplified by standard PCR from pCaST-rh3/4-norpA plasmids (kindly provided by Chi-Hon Lee) adding 5‘-end AatII- and 3‘-end NgoMIV-restriction sites. .. The resulting AatII/NgoMIV fragment was cloned into pBPnLsLexA::GADfluw (kindly provided by Gerald Rubin, Addgene plasmid #26232).

    Article Title: VGLUT modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration
    Article Snippet: This construct was introduced into the attP40 site on chromosome II by phiC31 injection (Genetivision, Houston, TX). .. The UAS>B3RT-STOP-B3RT-Luciferase strain was created by replacement of the myr::RFP sequence in pJFRC160 (Addgene plasmid #32139) with firefly luciferase. .. This modified construct was introduced into the attP2 site on chromosome III by phiC31 injection (Genetivision).

    Amplification:

    Article Title: Color Processing in the Early Visual System of Drosophila.
    Article Snippet: .. For LexAop-twich-2c a NotI/XbaI fragment including Twitch-2C DNA was isolated from pUAST-Twich-2c (see above) and cloned into pJFRC19-13XLexAop2-IVS-myr::GFP (kindly provided by Gerald Rubin, Addgene plasmid #26224) by exchanging myr::GFP. rh3, rh4, promoter regions were amplified by standard PCR from pCaST-rh3/4-norpA plasmids (kindly provided by Chi-Hon Lee) adding 5‘-end AatII- and 3‘-end NgoMIV-restriction sites. .. The resulting AatII/NgoMIV fragment was cloned into pBPnLsLexA::GADfluw (kindly provided by Gerald Rubin, Addgene plasmid #26232).

    Polymerase Chain Reaction:

    Article Title: Color Processing in the Early Visual System of Drosophila.
    Article Snippet: .. For LexAop-twich-2c a NotI/XbaI fragment including Twitch-2C DNA was isolated from pUAST-Twich-2c (see above) and cloned into pJFRC19-13XLexAop2-IVS-myr::GFP (kindly provided by Gerald Rubin, Addgene plasmid #26224) by exchanging myr::GFP. rh3, rh4, promoter regions were amplified by standard PCR from pCaST-rh3/4-norpA plasmids (kindly provided by Chi-Hon Lee) adding 5‘-end AatII- and 3‘-end NgoMIV-restriction sites. .. The resulting AatII/NgoMIV fragment was cloned into pBPnLsLexA::GADfluw (kindly provided by Gerald Rubin, Addgene plasmid #26232).

    Article Title: Synapses in oligodendrocyte precursor cells are dynamic and contribute to Ca 2+ activity
    Article Snippet: .. We generated pME_ATG-PSD-95-GFP-TAA and p3E_p2A-PSD-95-GFP-TGA-polyA. pME_myrGFP and pME_myrTagRFP-T were generated using PCR and BP reaction, using Fyn myristoylation and palmitoylation domain. pGP-CMV-GCaMP6s (Addgene 40753) was used as a template and 3 primers were used in a Q5 PCR (15 cycles at 71° C and 20 cycles at 72° C) to amplify GCaMP6s and add Kozak sequence, myr (Fyn myristoylation and palmitoylation domain), attB1, and attB2 sequences. ..

    other:

    Article Title: Color Processing in the Early Visual System of Drosophila.
    Article Snippet: The resulting AatII/NgoMIV fragment was cloned into pBPnLsLexA::GADfluw (kindly provided by Gerald Rubin, Addgene plasmid #26232).

    Sequencing:

    Article Title: VGLUT modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration
    Article Snippet: This construct was introduced into the attP40 site on chromosome II by phiC31 injection (Genetivision, Houston, TX). .. The UAS>B3RT-STOP-B3RT-Luciferase strain was created by replacement of the myr::RFP sequence in pJFRC160 (Addgene plasmid #32139) with firefly luciferase. .. This modified construct was introduced into the attP2 site on chromosome III by phiC31 injection (Genetivision).

    Article Title: Synapses in oligodendrocyte precursor cells are dynamic and contribute to Ca 2+ activity
    Article Snippet: .. We generated pME_ATG-PSD-95-GFP-TAA and p3E_p2A-PSD-95-GFP-TGA-polyA. pME_myrGFP and pME_myrTagRFP-T were generated using PCR and BP reaction, using Fyn myristoylation and palmitoylation domain. pGP-CMV-GCaMP6s (Addgene 40753) was used as a template and 3 primers were used in a Q5 PCR (15 cycles at 71° C and 20 cycles at 72° C) to amplify GCaMP6s and add Kozak sequence, myr (Fyn myristoylation and palmitoylation domain), attB1, and attB2 sequences. ..

    Luciferase:

    Article Title: VGLUT modulates sex differences in dopamine neuron vulnerability to age-related neurodegeneration
    Article Snippet: This construct was introduced into the attP40 site on chromosome II by phiC31 injection (Genetivision, Houston, TX). .. The UAS>B3RT-STOP-B3RT-Luciferase strain was created by replacement of the myr::RFP sequence in pJFRC160 (Addgene plasmid #32139) with firefly luciferase. .. This modified construct was introduced into the attP2 site on chromosome III by phiC31 injection (Genetivision).



    Similar Products

    active myr akt plasmid  (MedChemExpress)
    95
    MedChemExpress active myr akt plasmid
    Active Myr Akt Plasmid, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/Pictilisib/pm42458519-146-32-26
    Average 95 stars, based on 1 article reviews
    active myr akt plasmid - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    pt3 myr akt  (Addgene inc)
    94
    Addgene inc pt3 myr akt
    Pt3 Myr Akt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pT3-myr-AKT-HA+(Plasmid+%2331789)/pm41928626-184-4-5
    Average 94 stars, based on 1 article reviews
    pt3 myr akt - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    pt3 myr akt addgene  (Addgene inc)
    91
    Addgene inc pt3 myr akt addgene
    Pt3 Myr Akt Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pcDNA3%2E1%2FHisC-mTAZ+(Plasmid+%2331793)/pm41928626-296-167-168
    Average 91 stars, based on 1 article reviews
    pt3 myr akt addgene - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier
    pt3 ef1α hamyr akt  (Addgene inc)
    94
    Addgene inc pt3 ef1α hamyr akt
    Pt3 Ef1α Hamyr Akt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pT3-myr-AKT-HA+(Plasmid+%2331789)/pm41916296-289-18-20
    Average 94 stars, based on 1 article reviews
    pt3 ef1α hamyr akt - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    addgene pjfrc12 10xuas ivsmyr  (Addgene inc)
    93
    Addgene inc addgene pjfrc12 10xuas ivsmyr
    Addgene Pjfrc12 10xuas Ivsmyr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pJFRC12-10XUAS-IVS-myr%3A%3AGFP+(Plasmid+%2326222)/pm41803431-241-36-36
    Average 93 stars, based on 1 article reviews
    addgene pjfrc12 10xuas ivsmyr - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    myr aip  (Tocris)
    94
    Tocris myr aip
    ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 <t>or</t> <t>myr-AIP</t> for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.
    Myr Aip, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/Autocamtide-2-related+inhibitory+peptide%2C+myristoylated/bio_rxiv__64898__2026__02__27__708433-506-16-17
    Average 94 stars, based on 1 article reviews
    myr aip - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    myr ha akt1  (Addgene inc)
    93
    Addgene inc myr ha akt1
    ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 <t>or</t> <t>myr-AIP</t> for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.
    Myr Ha Akt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/1036+pcDNA3+Myr+HA+Akt1+(Plasmid+%239008)/pmc13010091-71-0-6
    Average 93 stars, based on 1 article reviews
    myr ha akt1 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    plasmid pjfrc19 13xlexaop2 ivs myr  (Addgene inc)
    95
    Addgene inc plasmid pjfrc19 13xlexaop2 ivs myr
    ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 <t>or</t> <t>myr-AIP</t> for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.
    Plasmid Pjfrc19 13xlexaop2 Ivs Myr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pJFRC19-13XLexAop2-IVS-myr%3A%3AGFP+(Plasmid+%2326224)/bio_rxiv__64898__2026__02__20__707075-163-10-12
    Average 95 stars, based on 1 article reviews
    plasmid pjfrc19 13xlexaop2 ivs myr - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    helper plasmid  (Addgene inc)
    93
    Addgene inc helper plasmid
    ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 <t>or</t> <t>myr-AIP</t> for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.
    Helper Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myr/pWZL+Neo+Myr+Flag+ACVRL1+(Plasmid+%2320413)/bio_rxiv__64898__2026__02__12__705519-201-12-14
    Average 93 stars, based on 1 article reviews
    helper plasmid - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 or myr-AIP for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.

    Journal: bioRxiv

    Article Title: Direct interaction of ribosomes with postsynaptic proteins gives rise to a privileged local synaptic translatome

    doi: 10.64898/2026.02.27.708433

    Figure Lengend Snippet: ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 or myr-AIP for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.

    Article Snippet: DIV 18-19 hippocampal neurons were treated with either 10 μM KN-93 (Tocris, #5215) or 10 μM myr-AIP (Tocris, #5959) for 30 min at 37°C, or left untreated.

    Techniques: Structural Proteomics, Binding Assay, Synthesized, Control, Labeling