mruby2 (Addgene inc)
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Structured Review
Addgene inc
mruby2
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+nt+c1/mRuby2-NT-C1+(Plasmid+%2354561)/pmc10997609-320-4-12
Average 92 stars, based on 12 article reviews
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+nt+c1/mRuby2-NT-C1+(Plasmid+%2354561)/pmc10997609-320-4-12
Average 92 stars, based on 12 article reviews
mruby2 - by Bioz Stars,
2026-09
92/100 stars
Images
1) Product Images from "Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells"
Article Title: Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells
Journal: Nature Communications
doi: 10.1038/s41467-024-47314-4
Figure Legend Snippet: A Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with mAID/mRuby2. B Order of events for the generation of the different cell lines. C Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. Experiments in each panel were performed at least three times, and the representative results are shown. D Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. E Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. The p value is calculated with one-way ANOVA and Tukey’s HSD test (* p < 0.05). Data are presented as mean values +/− SEM from biological triplicates. Source data are provided as a Source Data file.
Techniques Used: CRISPR, Western Blot, Biomarker Discovery, Fluorescence, DNA Methylation Assay, Liquid Chromatography with Mass Spectroscopy
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