Review



mpeg1 genes  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Addgene inc mpeg1 genes
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Mpeg1 Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/Tol2-mpeg1-dendra2+(Plasmid+%2351462)/pmc12771350-48-3-13
    Average 94 stars, based on 11 article reviews
    mpeg1 genes - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish ☆ "

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish ☆

    Journal: Current Research in Toxicology

    doi: 10.1016/j.crtox.2025.100276

    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Imaging, Transgenic Assay, Microscopy, Plasmid Preparation

    Related Articles

    Amplification:

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish
    Article Snippet: .. The Tol2 and mpeg1 genes were amplified from the Tol2-mpeg1-dendra2 plasmid ( ) (Addgene, Watertown, MA, USA) by inverse PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) and the primers mpeg1_infu_F1: 5′-GCG GCC GCG ACT CTA GAT-3′ and mpeg1_infu_R1: 5′-GGT GGC GGA TCC TTT TGC TG-3′. .. The coding region of mVenus was amplified from the mVenus N1 plasmid ( ) (Addgene) using PrimeSTAR Max DNA Polymerase and the mVenus_infu_F1: 5′-AAA GGA TCC GCC ACC ATG GTG AGC AAG GGC GAGG-3′ and mVenus_infu_R1: 5′-TAG AGT CGC GGC CGC TTA CTT GTA CAG CTC GTC CAT GCC-3′ primers.

    Plasmid Preparation:

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish
    Article Snippet: .. The Tol2 and mpeg1 genes were amplified from the Tol2-mpeg1-dendra2 plasmid ( ) (Addgene, Watertown, MA, USA) by inverse PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) and the primers mpeg1_infu_F1: 5′-GCG GCC GCG ACT CTA GAT-3′ and mpeg1_infu_R1: 5′-GGT GGC GGA TCC TTT TGC TG-3′. .. The coding region of mVenus was amplified from the mVenus N1 plasmid ( ) (Addgene) using PrimeSTAR Max DNA Polymerase and the mVenus_infu_F1: 5′-AAA GGA TCC GCC ACC ATG GTG AGC AAG GGC GAGG-3′ and mVenus_infu_R1: 5′-TAG AGT CGC GGC CGC TTA CTT GTA CAG CTC GTC CAT GCC-3′ primers.

    Inverse PCR:

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish
    Article Snippet: .. The Tol2 and mpeg1 genes were amplified from the Tol2-mpeg1-dendra2 plasmid ( ) (Addgene, Watertown, MA, USA) by inverse PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) and the primers mpeg1_infu_F1: 5′-GCG GCC GCG ACT CTA GAT-3′ and mpeg1_infu_R1: 5′-GGT GGC GGA TCC TTT TGC TG-3′. .. The coding region of mVenus was amplified from the mVenus N1 plasmid ( ) (Addgene) using PrimeSTAR Max DNA Polymerase and the mVenus_infu_F1: 5′-AAA GGA TCC GCC ACC ATG GTG AGC AAG GGC GAGG-3′ and mVenus_infu_R1: 5′-TAG AGT CGC GGC CGC TTA CTT GTA CAG CTC GTC CAT GCC-3′ primers.

    Activated Clotting Time Assay:

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish
    Article Snippet: .. The Tol2 and mpeg1 genes were amplified from the Tol2-mpeg1-dendra2 plasmid ( ) (Addgene, Watertown, MA, USA) by inverse PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) and the primers mpeg1_infu_F1: 5′-GCG GCC GCG ACT CTA GAT-3′ and mpeg1_infu_R1: 5′-GGT GGC GGA TCC TTT TGC TG-3′. .. The coding region of mVenus was amplified from the mVenus N1 plasmid ( ) (Addgene) using PrimeSTAR Max DNA Polymerase and the mVenus_infu_F1: 5′-AAA GGA TCC GCC ACC ATG GTG AGC AAG GGC GAGG-3′ and mVenus_infu_R1: 5′-TAG AGT CGC GGC CGC TTA CTT GTA CAG CTC GTC CAT GCC-3′ primers.



    Similar Products

    human mpeg1 expression plasmid  (Sino Biological)
    94
    Sino Biological human mpeg1 expression plasmid
    Human Mpeg1 Expression Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/Human+MPEG1+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-Flag+tag/pm41961488-272-1-10
    Average 94 stars, based on 1 article reviews
    human mpeg1 expression plasmid - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    mpeg1 genes  (Addgene inc)
    94
    Addgene inc mpeg1 genes
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Mpeg1 Genes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/Tol2-mpeg1-dendra2+(Plasmid+%2351462)/pmc12771350-48-3-13
    Average 94 stars, based on 1 article reviews
    mpeg1 genes - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    gene exp mpeg1 hs00909102 s1  (Thermo Fisher)
    94
    Thermo Fisher gene exp mpeg1 hs00909102 s1
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Gene Exp Mpeg1 Hs00909102 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/Gene+Exp%2E+MPEG1%2C+Hs00909102_s1/pm39915303-63-10-14
    Average 94 stars, based on 1 article reviews
    gene exp mpeg1 hs00909102 s1 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    gene exp mpeg1 mm01222137 g1  (Thermo Fisher)
    85
    Thermo Fisher gene exp mpeg1 mm01222137 g1
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Gene Exp Mpeg1 Mm01222137 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/Gene+Exp%2E+Mpeg1%2C+Mm01222137_g1/pm38528525-85-43-10
    Average 85 stars, based on 1 article reviews
    gene exp mpeg1 mm01222137 g1 - by Bioz Stars, 2026-11
    85/100 stars
    		  Buy from Supplier
    human mpeg1 gene for the soluble ectodomain (ncbi-gene id: 219972, nucleotide 49–1959)  (GenScript corporation)
    90
    GenScript corporation human mpeg1 gene for the soluble ectodomain (ncbi-gene id: 219972, nucleotide 49–1959)
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Human Mpeg1 Gene For The Soluble Ectodomain (Ncbi Gene Id: 219972, Nucleotide 49–1959), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/human+mpeg1+gene+for+the+soluble+ectodomain++ncbi+gene+id++219972++nucleotide+49+1959+/pmc06753088-131-7-15
    Average 90 stars, based on 1 article reviews
    human mpeg1 gene for the soluble ectodomain (ncbi-gene id: 219972, nucleotide 49–1959) - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    gene exp mpeg1 dr03439207 g1  (Thermo Fisher)
    86
    Thermo Fisher gene exp mpeg1 dr03439207 g1
    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg <t>(mpeg1:mVenus)</t> zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Gene Exp Mpeg1 Dr03439207 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mpeg1+genes/Gene+Exp%2E+mpeg1%2C+Dr03439207_g1/pmc04796261-137-46-68
    Average 86 stars, based on 1 article reviews
    gene exp mpeg1 dr03439207 g1 - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Current Research in Toxicology

    Article Title: Application to developmental toxicity testing of a novel method for whole-brain imaging of microglia in zebrafish ☆

    doi: 10.1016/j.crtox.2025.100276

    Figure Lengend Snippet: Whole-brain imaging of microglia in live transgenic zebrafish at 5–6 dpf by two-photon microscopy. (A) Left panel: Structure of the transposon vector used to generate Tg (mpeg1:mVenus) zebrafish. Center and right panels: Representative 2D image of a Z-plane (center) and 3D image of microglia (right) in whole Tg (mpeg1:mVenus) brain. (B) Representative Z-plane image of Tg (eno2:Cerulean) brain. (C) Representative Z-plane (left) and 3D image (right) of SHG of Tg (mpeg1:mVenus) brain used for voxelation. (D) Schematic of the voxelation approach to regional mapping of microglia in the telencephalon (yellow), mesencephalon (cyan), diencephalon (magenta), and metencephalon (orange) in Tg (mpeg1:mVenus) (left panel) and the microglial morphology with the same color-code (right panel). (E) Representative images of microglial morphology (color-coded as in C) in the whole brain of zebrafish exposed to EtOH (291 mM for 8 h) or VPA (2 mM for 24 h). Ro = rostral; Ca = caudal; Lt = left; Rt = righ.t. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The Tol2 and mpeg1 genes were amplified from the Tol2-mpeg1-dendra2 plasmid ( ) (Addgene, Watertown, MA, USA) by inverse PCR using PrimeSTAR Max DNA Polymerase (Takara Bio, Shiga, Japan) and the primers mpeg1_infu_F1: 5′-GCG GCC GCG ACT CTA GAT-3′ and mpeg1_infu_R1: 5′-GGT GGC GGA TCC TTT TGC TG-3′.

    Techniques: Imaging, Transgenic Assay, Microscopy, Plasmid Preparation