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mouse anti shank3  (NeuroMab)


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    Structured Review

    NeuroMab mouse anti shank3
    Mouse Anti Shank3, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+shank1/Anti-Shank1+And+Shank3+Antibody/pmc07769881-54-51-53
    Average 90 stars, based on 1 article reviews
    mouse anti shank3 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Loss of Protein Arginine Methyltransferase 8 Alters Synapse Composition and Function, Resulting in Behavioral Defects
    Article Snippet: .. Antibodies used included the following: mouse-PSD95 (NeuroMAB), mouse-HDAC2 (Abcam), rabbit-HA (Santa Cruz Biotechnology), mouse-Svp38 (Sigma), rabbit-Cacna1C (Novus), mouse-Syn1 (Synaptic Systems), rabbit-Nsf-1 (Thermo Fisher), rabbit-Syn2 (Abcam), rabbit-Syn3 (Synaptic Systems), rabbit-Syt7 (Abcam), mouse-Syt12 (NeuroMAB), rabbit-Cplx1 (Proteintech), mouse-β-actin (Sigma), rabbit-NR2A (Cell Signaling Technology), rabbit-NR2B (Cell Signaling Technology), mouse-NR1 (Millipore), mouse-GluA1 (Millipore), rabbit-GluA2 (Cell Signaling Technology), mouse-CaMKIIA (Millipore Bioscience Research Reagents), mouse-Shank1 (NeuroMAB), mouse-α-tubulin (Sigma), rabbit-Homer (GeneTex), rabbit-eIF4G1 (Cell Signaling Technology), rabbit-eIF4H (Cell Signaling Technology), rabbit-eIF4E (Cell Signaling Technology), and rabbit-FMRP (Cell Signaling Technology). qPCR. .. RNA was extracted from freshly dissected hippocampi using the QIAGEN RNeasy Plus Mini Kit. cDNA synthesis was performed with RNA to cDNA EcoDry Premix (Oligo dT) (Clontech). qPCR was performed with SsoFast EvaGreen Supermix (Bio-Rad) using a C1000 Thermal Cycler and a C96 Real-Time System (Bio-Rad).

    other:

    Article Title: Loss of Protein Arginine Methyltransferase 8 Alters Synapse Composition and Function, Resulting in Behavioral Defects
    Article Snippet: Antibodies used include: mouse-PSD95 159 (NeuroMAB), mouse-HDAC2 (Abcam), rabbit-HA (Santa Cruz), mouse-Svp38 160 8 (Sigma), rabbit-Cacna1C (Novus), mouse-Syn1 (Synaptic Systems), rabbit-Nsf-1 161 (Thermo Fisher), rabbit-Syn2 (Abcam), rabbit-Syn3 (Synaptic Systems), rabbit-Syt7 162 (Abcam), mouse-Syt12 (NeuroMAB), rabbit-Cplx1 (Proteintech), mouse-β-actin 163 (Sigma), rabbit-NR2A (Cell Signaling), rabbit-NR2B (Cell Signaling), mouse-NR1 164 (Millipore), mouse-GluA1 (Millipore), rabbit-GluA2 (Cell Signaling), mouse-CaMKIIA 165 (Chemicon), mouse-Shank1 (NeuroMAB), mouse-α-Tubulin (Sigma), rabbit-Homer 166 (GeneTex), rabbit-eIF4G1 (Cell Signaling), rabbit-eIF4H (Cell Signaling), rabbit-167 eIF4E (Cell Signaling), and rabbit-FMRP (Cell Signaling).



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    NeuroMab mouse monoclonal α-shank1
    Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of <t>SHANK1</t> ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM
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    NeuroMab mouse shank1
    Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of <t>SHANK1</t> ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM
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    Image Search Results


    Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of SHANK1 ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM

    Journal: Molecular Brain

    Article Title: Pre- and post-synaptic roles for DCC in memory consolidation in the adult mouse hippocampus

    doi: 10.1186/s13041-020-00597-2

    Figure Lengend Snippet: Alterations in dendritic spine morphology in CA1 pyramidal neurons from R4ag11-Cre/DCC fl/fl mice. a Representative camera lucida reconstructions of CA1 pyramidal neuron apical dendrites from control (left) and R4ag11-Cre/DCC fl/fl mice. Scale bar = 1 μm. b Group data show no significant difference in average spine density between control and R4ag11-Cre/DCC fl/fl mice (left), but significant reductions in average spine length and spine head width (right). c Dendritic spine type classification reveals a significant increase in number of stubby-type spines (R4ag11-Cre/DCC fl/fl : 55.8 ± 4.6%, Control: 29.6 ± 0.9%; t 5.4 = 5.59, p = 0.002) and significant decrease in the number of mushroom-type dendritic spines (R4ag11-Cre/DCC fl/fl : 40.6 ± 4.9%, Control: 68.2 ± 1.0%; t 5.47 = 5.40, p = 0.002), with no detectable differences in thin-type spines ( p = 0.41). d - i Group data (top) and representative Western blots from hippocampal homogenates of control and R4ag11-Cre/DCC fl/fl mice showing levels of SHANK1 ( d ), SHANK2 ( e ), SHANK3 ( f ), PSD-95 ( g ), p34-Arc ( h ), and phosphorylated S6 ribosomal protein ( i ). *: p < 0.05; **: p < 0.01. Data are presented as mean ± SEM

    Article Snippet: The primary antibodies used were: goat polyclonal anti-DCC A20 (1:1000; Santa Cruz Biotechnology, RRID: AB_2245770), rabbit α-GAPDH (1:1000; Santa Cruz Biotechnology, RRID: AB_10167668), mouse anti-β-tubulin III (1:5000; Promega, RRID: AB_430874), rabbit α-phospho-S6 ribosomal protein (1:1000, Cell Signaling, RRID: AB_331682), mouse monoclonal α-PSD-95 (1:200, NeuroMAB, RRID: AB_2292909), mouse monoclonal α-Shank1 (1:200, NeuroMAB, RRID: AB_10673108), mouse monoclonal α-Shank2 (1:200, NeuroMAB, RRID: AB_2254586), mouse monoclonal α-Shank3 (1:200, NeuroMAB, RRID: AB_2187730), and rabbit α-p34-Arc/ARPC2 (1:1000, Millipore, RRID: AB_310447).

    Techniques: Western Blot