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recombinant mouse rankl  (R&D Systems)


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    Structured Review

    R&D Systems recombinant mouse rankl
    Recombinant Mouse Rankl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+rankl/Recombinant+Mouse+TRANCE%2FRANK+L%2FTNFSF11+(E%2E+coli-expressed)/pmc13620161-289-6-9
    Average 96 stars, based on 303 article reviews
    recombinant mouse rankl - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis.
    Article Snippet: Before sorting, the cells were blocked with anti-mouse CD16/ CD32 antibodies (TruStain FcX, 101319, Biolegend, USA) diluted 1:200 for 30min on ice and stained with anti-mouse CD45 antibody (BD Horizon BV 421 Rat Anti-Mouse CD45 Clone 30-F11 (RUO), 563890, BD Biosciences, USA) diluted 1:100 for 1 h on ice to protect the tubes from light.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Functional and analytical recapitulation of osteoclast biology on demineralized bone paper
    Article Snippet: .. Osteoblasts conditioned medium were collected and analyzed OPG and RANKL secretion using an enzyme-linked immunosorbent assay (ELISA) kits for mouse OPG (DY459, R&D systems) and mouse RANKL (DY462, R&D systems). ..

    Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis
    Article Snippet: .. Levels of OPG and RANKL in conditioned media were measured via ELISA performed using an ELISA kit for mouse OPG (DY459, R&D systems, USA) and mouse RANKL (DY462, R&D systems, USA) according to the manufacturer’s instructions. ..

    Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis
    Article Snippet: .. Serum levels of P1NP, CTx1, TRAcP, OPG, RANKL, and TGF-β1 were measured via an enzyme-linked immunosorbent assay (ELISA) performed using ELISA kit for mouse P1NP (AC-33F1, Immunodiagnostic Systems, UK), mouse CTx1 (AC-06F1, Immunodiagnostic Systems, UK), mouse TRAcP 5b (SB-TR103, Immunodiagnostic Systems, UK), mouse OPG (MOP00, R&D systems, USA), mouse RANKL (MTR00, R&D systems, USA), and mouse TGF-β1 (DY1679-05 and DY007B, R&D systems, USA) according to the manufacturer’s instruction. ..

    Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis.
    Article Snippet: The crushed bone chips were washed four times with ice-cold DPBS and incubated with 5 mL of RBC lysis buffer (00- 4333-57, eBioscience, USA) for 5 min. Then, the crushed bone chips were subjected to three serial digestions with 5 mL of digestion medium (2 mg/mL of collagenase type I (C0130, SigmaAldrich, USA) and 2mg/mL of Dispase II (17105-041, Gibco, USA) in Hank’s balanced salt solution (14170112, Gibco, USA) at 37 °C 300 rcf for 20min each. .. Serum levels of P1NP, CTx1, TRAcP, OPG, RANKL, and TGF-β1 were measured via an enzyme-linked immunosorbent assay (ELISA) performed using ELISA kit for mouse P1NP (AC-33F1, Immunodiagnostic Systems, UK), mouse CTx1 (AC-06F1, Immunodiagnostic Systems, UK), mouse TRAcP 5b (SB-TR103, Immunodiagnostic Systems, UK), mouse OPG (MOP00, R&D systems, USA), mouse RANKL (MTR00, R&D systems, USA), and mouse TGF-β1 (DY167905 and DY007B, R&D systems, USA) according to the manufacturer’s instruction. ..

    Recombinant:

    Article Title: USP7 Inhibits Osteoclastogenesis via Dual Effects of Attenuating TRAF6/TAK1 Axis and Stimulating STING Signaling
    Article Snippet: CCK-8 (Cell Counting Kit-8) was obtained from Dojindo Molecular Technology (Kumamoto, Japan). .. Recombinant soluble mouse M-CSF and mouse RANKL were purchased from R&D Systems (Minneapolis, MN, USA). .. Specific antibodies anti-phosphor-p38, anti-total-p38, anti-phosphor-ERK, anti-total-ERK, anti-phosphor-JNK, anti-total-JNK, anti-phosphor-p65, anti-total-p65, anti-phosphor-TAK1, anti-total-TAK1, anti-IκBα, anti-TRAF6, anti-USP7, anti-Ubiquitin, anti-c-Fos, anti-NFATc1, anti-TRAP, anti-Flag, anti-HA, anti-Histone, anti-Myc, anti-β-actin and anti-β-tubulin were obtained from Cell Signal Technology (Shanghai, China) and Abcam (Shanghai, China), the detailed information of antibodies was shown in .

    Article Title: Inhibitory effect of hydroxychloroquine on glucocorticoid‐induced osteoporosis in lupus therapy
    Article Snippet: The following antibodies were used: PE‐conjugated anti‐CD64 (X54‐5/7.1, Cat. 139303; Biolegend, California, USA), FITC‐conjugated anti‐F4/80 (CI: A3‐1, Cat. E‐AB‐F0995C; Elabscience, Wuhan, China), anti‐phospho‐NF‐κB p65 (Ser536) (Cat. 3033; Cell Signaling Technology, Boston, USA), anti‐NF‐κB p65 (10745‐1‐AP; Proteintech, Wuhan, China), anti‐human IgG (Abcam, Cambridge, UK), anti‐NRF2 (Cat. A21508; ABclonal, Wuhan, China) and anti‐GAPDH (Cat. AC033; ABclonal, Wuhan, China). .. Recombinant mouse M‐CSF (416‐ML; R&D, California, USA) and mouse RANKL (462‐TEC; R&D, California, USA) proteins were used. .. MEM α (Gibco, California, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, California, USA) and H2DCFDA dye (Cat. HY‐D0940; MCE, New Jersey, USA) was used.

    Article Title: Metal-polyDNA nanoparticles reconstruct osteoporotic microenvironment for enhanced osteoporosis treatment.
    Article Snippet: MATERIALS AND METHODS Materials, cell, and animal Calcium chloride [CaCl2; Analytical reagent (AR), >99%], ammonium sulfate [(NH4)2SO4; AR, 99%], 1,4-dithio-DL-threitol (DTT; biotechnology grade, 99.5%), CCK-8, Hoechst 33342, Dulbecco’s modified Eagle’s medium (DMEM), α-minimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin, streptomycin, trypsin, and chemicals for TRAP stain were obtained from Sigma-Aldrich (St. Louis, USA). .. Recombinant mouse M-CSF and mouse RANKL were obtained from R&D Systems (Minneapolis, MN, USA). .. T4 DNA ligase (20,000 U) and phi29 DNA polymerase (1250 U) were purchased from Beyotime Institute of Biotechnology (Shanghai, China).



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    R&D Systems mouse rankl
    Dual inhibition of TGF-β and sclerostin increases bone mass in a hindlimb unloading model. a Experimental timeline of hindlimb unloading using C57B1/6 J mice and treatment with TGFβ-Ab, Scl-Ab, or both. Mice euthanized at week 12. b Representative μCT images of femoral diaphysis. c Representative confocal images of trabecular bone showing calcein (green), alizarin (red) signals, and DAPI (blue). Scale bar = 100 μm. d Representative images of TRAP-stained femur sections; violet = TRAP-positive, green = counterstain. Scale bar = 200 μm. e – h Bar plot of trabecular bone volume/total volume (Tra. BV/TV, e ), trabecular thickness (Tb. Th, f ), trabecular separation (Tb. Sp, g ), and trabecular number (Tb. N, h ) from μCT. Data = mean ± standard deviation. i , j Bar plot of mineral apposition rate (Tra. MAR, i ) and trabecular bone formation rate per bone surface (Tra. BFR/BS, j ) from labeling images. Data = mean ± standard deviation k Box plot of TRAP+ osteoclasts per bone surface (OC.N/BS), measured through ImageJ from TRAP-stained slides. l – n Box plot of serum P1NP ( l ) and TRAP ( m ) levels, <t>and</t> <t>RANKL/OPG</t> ratio ( n ). Group sizes: Control ( n = 5), Un_con ( n = 5), Scl-Ab ( n = 6), TGFβ-Ab ( n = 6), and Scl-Ab+TGFβ-Ab ( n = 7). Each point represents an individual mouse. ANOVA was used for statistics; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; ns not significant
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    Dual inhibition of TGF-β and sclerostin increases bone mass in a hindlimb unloading model. a Experimental timeline of hindlimb unloading using C57B1/6 J mice and treatment with TGFβ-Ab, Scl-Ab, or both. Mice euthanized at week 12. b Representative μCT images of femoral diaphysis. c Representative confocal images of trabecular bone showing calcein (green), alizarin (red) signals, and DAPI (blue). Scale bar = 100 μm. d Representative images of TRAP-stained femur sections; violet = TRAP-positive, green = counterstain. Scale bar = 200 μm. e – h Bar plot of trabecular bone volume/total volume (Tra. BV/TV, e ), trabecular thickness (Tb. Th, f ), trabecular separation (Tb. Sp, g ), and trabecular number (Tb. N, h ) from μCT. Data = mean ± standard deviation. i , j Bar plot of mineral apposition rate (Tra. MAR, i ) and trabecular bone formation rate per bone surface (Tra. BFR/BS, j ) from labeling images. Data = mean ± standard deviation k Box plot of TRAP+ osteoclasts per bone surface (OC.N/BS), measured through ImageJ from TRAP-stained slides. l – n Box plot of serum P1NP ( l ) and TRAP ( m ) levels, <t>and</t> <t>RANKL/OPG</t> ratio ( n ). Group sizes: Control ( n = 5), Un_con ( n = 5), Scl-Ab ( n = 6), TGFβ-Ab ( n = 6), and Scl-Ab+TGFβ-Ab ( n = 7). Each point represents an individual mouse. ANOVA was used for statistics; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; ns not significant
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    Image Search Results


    Dual inhibition of TGF-β and sclerostin increases bone mass in a hindlimb unloading model. a Experimental timeline of hindlimb unloading using C57B1/6 J mice and treatment with TGFβ-Ab, Scl-Ab, or both. Mice euthanized at week 12. b Representative μCT images of femoral diaphysis. c Representative confocal images of trabecular bone showing calcein (green), alizarin (red) signals, and DAPI (blue). Scale bar = 100 μm. d Representative images of TRAP-stained femur sections; violet = TRAP-positive, green = counterstain. Scale bar = 200 μm. e – h Bar plot of trabecular bone volume/total volume (Tra. BV/TV, e ), trabecular thickness (Tb. Th, f ), trabecular separation (Tb. Sp, g ), and trabecular number (Tb. N, h ) from μCT. Data = mean ± standard deviation. i , j Bar plot of mineral apposition rate (Tra. MAR, i ) and trabecular bone formation rate per bone surface (Tra. BFR/BS, j ) from labeling images. Data = mean ± standard deviation k Box plot of TRAP+ osteoclasts per bone surface (OC.N/BS), measured through ImageJ from TRAP-stained slides. l – n Box plot of serum P1NP ( l ) and TRAP ( m ) levels, and RANKL/OPG ratio ( n ). Group sizes: Control ( n = 5), Un_con ( n = 5), Scl-Ab ( n = 6), TGFβ-Ab ( n = 6), and Scl-Ab+TGFβ-Ab ( n = 7). Each point represents an individual mouse. ANOVA was used for statistics; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; ns not significant

    Journal: Bone Research

    Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis

    doi: 10.1038/s41413-026-00521-9

    Figure Lengend Snippet: Dual inhibition of TGF-β and sclerostin increases bone mass in a hindlimb unloading model. a Experimental timeline of hindlimb unloading using C57B1/6 J mice and treatment with TGFβ-Ab, Scl-Ab, or both. Mice euthanized at week 12. b Representative μCT images of femoral diaphysis. c Representative confocal images of trabecular bone showing calcein (green), alizarin (red) signals, and DAPI (blue). Scale bar = 100 μm. d Representative images of TRAP-stained femur sections; violet = TRAP-positive, green = counterstain. Scale bar = 200 μm. e – h Bar plot of trabecular bone volume/total volume (Tra. BV/TV, e ), trabecular thickness (Tb. Th, f ), trabecular separation (Tb. Sp, g ), and trabecular number (Tb. N, h ) from μCT. Data = mean ± standard deviation. i , j Bar plot of mineral apposition rate (Tra. MAR, i ) and trabecular bone formation rate per bone surface (Tra. BFR/BS, j ) from labeling images. Data = mean ± standard deviation k Box plot of TRAP+ osteoclasts per bone surface (OC.N/BS), measured through ImageJ from TRAP-stained slides. l – n Box plot of serum P1NP ( l ) and TRAP ( m ) levels, and RANKL/OPG ratio ( n ). Group sizes: Control ( n = 5), Un_con ( n = 5), Scl-Ab ( n = 6), TGFβ-Ab ( n = 6), and Scl-Ab+TGFβ-Ab ( n = 7). Each point represents an individual mouse. ANOVA was used for statistics; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.000 1; ns not significant

    Article Snippet: Serum levels of P1NP, CTx1, TRAcP, OPG, RANKL, and TGF-β1 were measured via an enzyme-linked immunosorbent assay (ELISA) performed using ELISA kit for mouse P1NP (AC-33F1, Immunodiagnostic Systems, UK), mouse CTx1 (AC-06F1, Immunodiagnostic Systems, UK), mouse TRAcP 5b (SB-TR103, Immunodiagnostic Systems, UK), mouse OPG (MOP00, R&D systems, USA), mouse RANKL (MTR00, R&D systems, USA), and mouse TGF-β1 (DY1679-05 and DY007B, R&D systems, USA) according to the manufacturer’s instruction.

    Techniques: Inhibition, Staining, Standard Deviation, Labeling, Control