recombinant mouse rankl (R&D Systems)
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Recombinant Mouse Rankl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+rankl/Recombinant+Mouse+TRANCE%2FRANK+L%2FTNFSF11+(E%2E+coli-expressed)/pmc13620161-289-6-9
Average 96 stars, based on 303 article reviews
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other:Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis. Article Snippet: Before sorting, the cells were blocked with anti-mouse CD16/ CD32 antibodies (TruStain FcX, 101319, Biolegend, USA) diluted 1:200 for 30min on ice and stained with anti-mouse CD45 antibody (BD Horizon BV 421 Rat Anti-Mouse CD45 Clone 30-F11 (RUO), 563890, BD Biosciences, USA) diluted 1:100 for 1 h on ice to protect the tubes from light. Enzyme-linked Immunosorbent Assay:Article Title: Functional and analytical recapitulation of osteoclast biology on demineralized bone paper Article Snippet: .. Osteoblasts conditioned medium were collected and analyzed OPG and RANKL secretion using an enzyme-linked immunosorbent assay (ELISA) kits for mouse OPG (DY459, R&D systems) and Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis Article Snippet: .. Levels of OPG and RANKL in conditioned media were measured via ELISA performed using an ELISA kit for mouse OPG (DY459, R&D systems, USA) and Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis Article Snippet: .. Serum levels of P1NP, CTx1, TRAcP, OPG, RANKL, and TGF-β1 were measured via an enzyme-linked immunosorbent assay (ELISA) performed using ELISA kit for mouse P1NP (AC-33F1, Immunodiagnostic Systems, UK), mouse CTx1 (AC-06F1, Immunodiagnostic Systems, UK), mouse TRAcP 5b (SB-TR103, Immunodiagnostic Systems, UK), mouse OPG (MOP00, R&D systems, USA), Article Title: Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosis. Article Snippet: The crushed bone chips were washed four times with ice-cold DPBS and incubated with 5 mL of RBC lysis buffer (00- 4333-57, eBioscience, USA) for 5 min. Then, the crushed bone chips were subjected to three serial digestions with 5 mL of digestion medium (2 mg/mL of collagenase type I (C0130, SigmaAldrich, USA) and 2mg/mL of Dispase II (17105-041, Gibco, USA) in Hank’s balanced salt solution (14170112, Gibco, USA) at 37 °C 300 rcf for 20min each. .. Serum levels of P1NP, CTx1, TRAcP, OPG, RANKL, and TGF-β1 were measured via an enzyme-linked immunosorbent assay (ELISA) performed using ELISA kit for mouse P1NP (AC-33F1, Immunodiagnostic Systems, UK), mouse CTx1 (AC-06F1, Immunodiagnostic Systems, UK), mouse TRAcP 5b (SB-TR103, Immunodiagnostic Systems, UK), mouse OPG (MOP00, R&D systems, USA), Recombinant:Article Title: USP7 Inhibits Osteoclastogenesis via Dual Effects of Attenuating TRAF6/TAK1 Axis and Stimulating STING Signaling Article Snippet: CCK-8 (Cell Counting Kit-8) was obtained from Dojindo Molecular Technology (Kumamoto, Japan). .. Recombinant soluble mouse M-CSF and Article Title: Inhibitory effect of hydroxychloroquine on glucocorticoid‐induced osteoporosis in lupus therapy Article Snippet: The following antibodies were used: PE‐conjugated anti‐CD64 (X54‐5/7.1, Cat. 139303; Biolegend, California, USA), FITC‐conjugated anti‐F4/80 (CI: A3‐1, Cat. E‐AB‐F0995C; Elabscience, Wuhan, China), anti‐phospho‐NF‐κB p65 (Ser536) (Cat. 3033; Cell Signaling Technology, Boston, USA), anti‐NF‐κB p65 (10745‐1‐AP; Proteintech, Wuhan, China), anti‐human IgG (Abcam, Cambridge, UK), anti‐NRF2 (Cat. A21508; ABclonal, Wuhan, China) and anti‐GAPDH (Cat. AC033; ABclonal, Wuhan, China). .. Recombinant mouse M‐CSF (416‐ML; R&D, California, USA) and Article Title: Metal-polyDNA nanoparticles reconstruct osteoporotic microenvironment for enhanced osteoporosis treatment. Article Snippet: MATERIALS AND METHODS Materials, cell, and animal Calcium chloride [CaCl2; Analytical reagent (AR), >99%], ammonium sulfate [(NH4)2SO4; AR, 99%], 1,4-dithio-DL-threitol (DTT; biotechnology grade, 99.5%), CCK-8, Hoechst 33342, Dulbecco’s modified Eagle’s medium (DMEM), α-minimum essential medium (α-MEM), fetal bovine serum (FBS), penicillin, streptomycin, trypsin, and chemicals for TRAP stain were obtained from Sigma-Aldrich (St. Louis, USA). .. Recombinant mouse M-CSF and |
