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Arraystar inc mouse lncrna microarray v2.0
Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a <t>microarray</t> experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.
Mouse Lncrna Microarray V2.0, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice

Journal: Biomedicines

doi: 10.3390/biomedicines12122703

Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a microarray experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.
Figure Legend Snippet: Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a microarray experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.

Techniques Used: Sequencing, Control, Microarray, Expressing

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Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
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Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
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Derivative Assay:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
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Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
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Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Fluorescence:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
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Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
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Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Imaging:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Over Expression:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Plasmid Preparation:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Knock-Out:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Mouse Assay:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Isolation:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Gene Expression:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Quantitative Proteomics:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Comparison:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.

Real-time Polymerase Chain Reaction:

Article Title: LncRNA-AK012226 Is Involved in Fat Accumulation in db/db Mice Fatty Liver and Non-alcoholic Fatty Liver Disease Cell Model
Article Snippet: Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) was used to study the profiles of mouse lncRNAs and protein-coding transcripts in the liver.



Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: To identify lncRNA candidates deregulated in whole heart samples 6 weeks after TAC (transverse aortic constriction) or in cardiomyocytes from mouse hearts 13 weeks after TAC general lncRNA profiling provided by Arraystar was performed (Arraystar Mouse LncRNA microarray V2.0).

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: TABLE 8 H19 IncRNA derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice.

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice
Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Article Title: LncRNAs for therapy and diagnosis of cardiac hypertrophy
Article Snippet: The following Tables 3 and 4 and FIGS. 14 and 15 summarize candidate lncRNAs that have been validated from different Microarrays: TABLE 4 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing whole heart samples from 6 week sham and TAC mice. related Regu- Name Identifier Source Size relationship gene FC lation Gm16192 ENSMUST00000148357 Ensembl 937 bp antisense Mtus1 4, 20 Up Gm11641 ENSMUST00000130556 Ensembl 923 bp antisense Arhgap27 3, 85 Up Gm8459 ENSMUST00000162504 Ensembl 760 bp intergenic — −10, 09 Down AK083183 AK083183.1 Fantom3 2759 bp intergenic — −8, 86 Down Gm13316 ENSMUST00000140537 Ensembl 691 bp antisense Cacnb2 −2, 83 Down H19 ENSMUST00000136359 Ensembl 2286 bp intergenic — −2, 01 Down (NR_001592.1) ENSMUST00000152754 Ensembl 1853 bp intergenic — −2, 06 Down ENSMUST00000140716 Ensembl 817 bp Intergenic — −5, 30 Down TABLE 5 LncNA candidates derived from Arraystar Mouse LncRNA microarray V2.0 analyzing a cardiomyocyte-specific fraction of 12 week healthy vs. 13 week TAC mouse hearts. related Regula Name Identifier Source Size relationship gene FC tion AK013700 AK013700.1 predicted 725 bp intergenic — 10, 93 Up Gm15892- ENSMUST00000 Ensembl 439 bp intergenic — 9, 54 Up 002 152627 BCO23483 uc007dvi.1 UCSC 1726 bp intergenic — 6, 52 Up Gm12224- ENSMUST00000 Ensembl 567 bp antisense AcsI6 6, 20 Up 001 124047 AJ409495 AJ409495.1 NRED 292 bp intergenic — 5, 80 Up Gm16192 ENSMUST00000 Ensembl 937 bp antisense Mtus1 5, 50 Up 148357 Gm8822- ENSMUST00000 Ensembl 339 bp intergenic — −10, 12 Down 202 168371 H19 ENSMUST00000 Ensembl 817 bp intergenic — −4, 73 Down 140716 (NR_001592.1) ENSMUST00000 Ensem bl 2286 bp intergenic — −2, 48 Down 136359

Article Title: Gm9795 Promotes Inflammation in Non-Alcoholic Steatohepatitis via NF-κB/JNK Pathway by Endoplasmic Reticulum Stress
Article Snippet: 9 Results: Expression Profifiles of lncRNAs and mRNAs in Mice With NAFLD and NASH To determine the expression profifiles of lncRNAs and mRNAs in mice with NAFLD and NASH, the Mouse LncRNA Microarray V2.0 (Arraystar, Rockville, MD, United States) were used.



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<t>LncRNA</t> AK018453 is upregulated and regulates TRPA1 expression in activated astrocytes by IL‐17. (a) Comparative lncRNA array analysis indicated that differentially upregulated lncRNAs occurred in the primary astrocytes treated with IL‐17 (50 ng/ml) at 3 and 6 h (2.0‐fold higher than DMEM group). (b) Hot map showed some differentially‐expressed lncRNAs in astrocytes stimulated with IL‐17. Red box circled differentially co‐upregulated lncRNA AK018453. (c) Real‐time PCR assay was employed to verify lncRNA AK018453 expression in primary mouse astrocytes treated by IL‐17 and spinal cords from EAE mice. Results were represented as mean ± SEM. ** p < .01 and *** p < .001 versus 0 h group. # p < .05 and ## p < .01 versus NC group ( n = 3). (d and e) RIP assay was performed to investigate the interaction of AK018453 with NF‐κB p65 and CBP/P300 in astrocytes stimulated with IL‐17 for 6 h, or prior to infection by lentiviruses AK018453‐shRNA (AK‐shRNA) and ctrl‐shRNA for 72 h. The data were from three independent experiments. ** p < .01 and *** p < .001 versus DMEM. # p < .05 versus ctrl‐shRNA + IL‐17 group ( n = 3). (f and g) ChIP assay analysis of NF‐κB p65, CBP/P300, H3K27ac, and RNA pol II enrichment on the promoter of TRAP1 gene in astrocytes stimulation with IL‐17 for 6 h, or prior to infection by lentiviruses AK‐shRNA and ctrl‐shRNA for 72 h. The data were from three independent experiments and represented as mean ± SEM. * p < .05, ** p < .01, *** p < .001 versus DMEM; # p < .05 and ## p < .01 versus ctrl‐shRNA + IL‐17 group ( n = 3)
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Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a microarray experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.

Journal: Biomedicines

Article Title: Generation of Transcript Length Variants and Reprogramming of mRNA Splicing During Atherosclerosis Progression in ApoE-Deficient Mice

doi: 10.3390/biomedicines12122703

Figure Lengend Snippet: Pipeline of the experimental approach followed. Female ApoE -/- mice were treated twice weekly for 16 weeks (arrowheads) with an intraperitoneal administration of 50 μg of an αCD40 siRNA (treatment group, T) or 50 μg of a scrambled sequence siRNA (control group, C). Aortic tissue was extracted at weeks 8 (basal) and 10 and 24 for both experimental groups (C10, C24, T10 and T24). Total RNA was extracted and used for a microarray experiment in which expression data were normalized to the basal levels at week 8. Only downregulated transcripts of the C and T groups were used in this analysis of length dynamics (see text). In parenthesis is the number of transcripts from each group. Transcripts simultaneously expressed in two experimental conditions (C10/C24 and T10/T24) were identified and used for bioinformatic analysis of the mechanisms regulating transcript length variation.

Article Snippet: In this work, we used the “Arraystar Mouse LncRNA Microarray v2.0” from Arraystar Inc. (Rockville, MD, USA) on a custom basis.

Techniques: Sequencing, Control, Microarray, Expressing

LncRNA AK018453 is upregulated and regulates TRPA1 expression in activated astrocytes by IL‐17. (a) Comparative lncRNA array analysis indicated that differentially upregulated lncRNAs occurred in the primary astrocytes treated with IL‐17 (50 ng/ml) at 3 and 6 h (2.0‐fold higher than DMEM group). (b) Hot map showed some differentially‐expressed lncRNAs in astrocytes stimulated with IL‐17. Red box circled differentially co‐upregulated lncRNA AK018453. (c) Real‐time PCR assay was employed to verify lncRNA AK018453 expression in primary mouse astrocytes treated by IL‐17 and spinal cords from EAE mice. Results were represented as mean ± SEM. ** p < .01 and *** p < .001 versus 0 h group. # p < .05 and ## p < .01 versus NC group ( n = 3). (d and e) RIP assay was performed to investigate the interaction of AK018453 with NF‐κB p65 and CBP/P300 in astrocytes stimulated with IL‐17 for 6 h, or prior to infection by lentiviruses AK018453‐shRNA (AK‐shRNA) and ctrl‐shRNA for 72 h. The data were from three independent experiments. ** p < .01 and *** p < .001 versus DMEM. # p < .05 versus ctrl‐shRNA + IL‐17 group ( n = 3). (f and g) ChIP assay analysis of NF‐κB p65, CBP/P300, H3K27ac, and RNA pol II enrichment on the promoter of TRAP1 gene in astrocytes stimulation with IL‐17 for 6 h, or prior to infection by lentiviruses AK‐shRNA and ctrl‐shRNA for 72 h. The data were from three independent experiments and represented as mean ± SEM. * p < .05, ** p < .01, *** p < .001 versus DMEM; # p < .05 and ## p < .01 versus ctrl‐shRNA + IL‐17 group ( n = 3)

Journal: Glia

Article Title: The LncRNA AK018453 regulates TRAP1/Smad signaling in IL‐17‐activated astrocytes: A potential role in EAE pathogenesis

doi: 10.1002/glia.24239

Figure Lengend Snippet: LncRNA AK018453 is upregulated and regulates TRPA1 expression in activated astrocytes by IL‐17. (a) Comparative lncRNA array analysis indicated that differentially upregulated lncRNAs occurred in the primary astrocytes treated with IL‐17 (50 ng/ml) at 3 and 6 h (2.0‐fold higher than DMEM group). (b) Hot map showed some differentially‐expressed lncRNAs in astrocytes stimulated with IL‐17. Red box circled differentially co‐upregulated lncRNA AK018453. (c) Real‐time PCR assay was employed to verify lncRNA AK018453 expression in primary mouse astrocytes treated by IL‐17 and spinal cords from EAE mice. Results were represented as mean ± SEM. ** p < .01 and *** p < .001 versus 0 h group. # p < .05 and ## p < .01 versus NC group ( n = 3). (d and e) RIP assay was performed to investigate the interaction of AK018453 with NF‐κB p65 and CBP/P300 in astrocytes stimulated with IL‐17 for 6 h, or prior to infection by lentiviruses AK018453‐shRNA (AK‐shRNA) and ctrl‐shRNA for 72 h. The data were from three independent experiments. ** p < .01 and *** p < .001 versus DMEM. # p < .05 versus ctrl‐shRNA + IL‐17 group ( n = 3). (f and g) ChIP assay analysis of NF‐κB p65, CBP/P300, H3K27ac, and RNA pol II enrichment on the promoter of TRAP1 gene in astrocytes stimulation with IL‐17 for 6 h, or prior to infection by lentiviruses AK‐shRNA and ctrl‐shRNA for 72 h. The data were from three independent experiments and represented as mean ± SEM. * p < .05, ** p < .01, *** p < .001 versus DMEM; # p < .05 and ## p < .01 versus ctrl‐shRNA + IL‐17 group ( n = 3)

Article Snippet: In brief, Mouse LncRNA Microarray v2.0 (8 × 60K, Arraystar) was performed by Kangchen Bio‐tech (Shanghai, China) to measure the global profiling of mouse LncRNAs in primary mouse astrocytes treated with or without IL‐17.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Infection, shRNA

AK018453 knockdown lessens the pathology of EAE mice. On day 7 after administration with recombinant lentivirus carrying GFAP promoter and AK‐shRNA or ctrl‐shRNA sequence, respectively, mice were immunized with or without MOG 35–55 for 21 days. (a) The level of lncRNA AK018453 was determined by real‐time PCR assay. *** p < .001 versus NC group; # p < .05 versus ctrl‐shRNA group ( n = 10 mice/group). (b) The clinical scores for EAE mice infected with AK‐shRNA or ctrl‐shRNA ( n = 10 mice per group). *** p < .001 versus NC group; ## p < .01 versus ctrl‐shRNA group. (c) Western blot assay was used to determine the protein levels of TRAP1, Smad4, p‐Smad2/3, Smad2/3, and GFAP in the spinal cords. (d and e) The production of TNF‐α, CXCL10, and MCP‐1 in the spinal cords and peripheral blood from the AK‐shRNA and ctrl‐shRNA mice was measured by real‐time PCR assay and ELISA assay, respectively. The data are represented as mean ± SEM. *** p < .001 versus NC group; # p < .05, ## p < .01, and ### p < .001 versus ctrl‐shRNA group ( n = 10 mice/group). (f) Hematoxylin and eosin (H&E) staining was designed to observe the infiltrations of inflammatory cells in spinal cords (scale bars, 50 μm). (g) Luxol fast blue (LFB) staining was utilized to test the medullary sheath damages from spinal cords (scale bars, 50 μm). Red box areas in the upper rows are presented enlarged underneath. (h) Electron microscope (EM) was employed to investigate the severe disruption or mild loosening of the medullary sheath in spinal cords. Red arrow shows the changes of the medullary sheath. Scale bars, 1 μm

Journal: Glia

Article Title: The LncRNA AK018453 regulates TRAP1/Smad signaling in IL‐17‐activated astrocytes: A potential role in EAE pathogenesis

doi: 10.1002/glia.24239

Figure Lengend Snippet: AK018453 knockdown lessens the pathology of EAE mice. On day 7 after administration with recombinant lentivirus carrying GFAP promoter and AK‐shRNA or ctrl‐shRNA sequence, respectively, mice were immunized with or without MOG 35–55 for 21 days. (a) The level of lncRNA AK018453 was determined by real‐time PCR assay. *** p < .001 versus NC group; # p < .05 versus ctrl‐shRNA group ( n = 10 mice/group). (b) The clinical scores for EAE mice infected with AK‐shRNA or ctrl‐shRNA ( n = 10 mice per group). *** p < .001 versus NC group; ## p < .01 versus ctrl‐shRNA group. (c) Western blot assay was used to determine the protein levels of TRAP1, Smad4, p‐Smad2/3, Smad2/3, and GFAP in the spinal cords. (d and e) The production of TNF‐α, CXCL10, and MCP‐1 in the spinal cords and peripheral blood from the AK‐shRNA and ctrl‐shRNA mice was measured by real‐time PCR assay and ELISA assay, respectively. The data are represented as mean ± SEM. *** p < .001 versus NC group; # p < .05, ## p < .01, and ### p < .001 versus ctrl‐shRNA group ( n = 10 mice/group). (f) Hematoxylin and eosin (H&E) staining was designed to observe the infiltrations of inflammatory cells in spinal cords (scale bars, 50 μm). (g) Luxol fast blue (LFB) staining was utilized to test the medullary sheath damages from spinal cords (scale bars, 50 μm). Red box areas in the upper rows are presented enlarged underneath. (h) Electron microscope (EM) was employed to investigate the severe disruption or mild loosening of the medullary sheath in spinal cords. Red arrow shows the changes of the medullary sheath. Scale bars, 1 μm

Article Snippet: In brief, Mouse LncRNA Microarray v2.0 (8 × 60K, Arraystar) was performed by Kangchen Bio‐tech (Shanghai, China) to measure the global profiling of mouse LncRNAs in primary mouse astrocytes treated with or without IL‐17.

Techniques: Knockdown, Recombinant, shRNA, Sequencing, Real-time Polymerase Chain Reaction, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, Microscopy, Disruption

Schematic representation of lncRNA AK018453 contributing to the pathology of EAE mice via regulating TRAP1/Smad pathway. Response to IL‐17 stimulation, AK018453 is upregulated in astrocytes, which regulates epigenetically the expression of TRAP1 through interacting with CBP/P300, in turn promotes the production of pro‐inflammatory cytokines such as TNF‐α, CXCL10, and MCP‐1, thus aggravating the EAE progression

Journal: Glia

Article Title: The LncRNA AK018453 regulates TRAP1/Smad signaling in IL‐17‐activated astrocytes: A potential role in EAE pathogenesis

doi: 10.1002/glia.24239

Figure Lengend Snippet: Schematic representation of lncRNA AK018453 contributing to the pathology of EAE mice via regulating TRAP1/Smad pathway. Response to IL‐17 stimulation, AK018453 is upregulated in astrocytes, which regulates epigenetically the expression of TRAP1 through interacting with CBP/P300, in turn promotes the production of pro‐inflammatory cytokines such as TNF‐α, CXCL10, and MCP‐1, thus aggravating the EAE progression

Article Snippet: In brief, Mouse LncRNA Microarray v2.0 (8 × 60K, Arraystar) was performed by Kangchen Bio‐tech (Shanghai, China) to measure the global profiling of mouse LncRNAs in primary mouse astrocytes treated with or without IL‐17.

Techniques: Expressing